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Protein

Neuronal acetylcholine receptor subunit beta-3

Gene

Chrnb3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-629594. Highly calcium permeable postsynaptic nicotinic acetylcholine receptors.
R-MMU-629597. Highly calcium permeable nicotinic acetylcholine receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal acetylcholine receptor subunit beta-3
Gene namesi
Name:Chrnb3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:106212. Chrnb3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini31 – 238ExtracellularSequence analysisAdd BLAST208
Transmembranei239 – 263HelicalSequence analysisAdd BLAST25
Transmembranei271 – 288HelicalSequence analysisAdd BLAST18
Transmembranei305 – 326HelicalSequence analysisAdd BLAST22
Topological domaini327 – 434CytoplasmicSequence analysisAdd BLAST108
Transmembranei435 – 453HelicalSequence analysisAdd BLAST19

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3350222.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30Sequence analysisAdd BLAST30
ChainiPRO_000000038431 – 464Neuronal acetylcholine receptor subunit beta-3Add BLAST434

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi55N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi159 ↔ 173By similarity
Glycosylationi172N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8BMN3.
PRIDEiQ8BMN3.

PTM databases

iPTMnetiQ8BMN3.
PhosphoSitePlusiQ8BMN3.

Expressioni

Gene expression databases

BgeeiENSMUSG00000031492.
ExpressionAtlasiQ8BMN3. baseline and differential.
GenevisibleiQ8BMN3. MM.

Interactioni

Subunit structurei

Neuronal AChR seems to be composed of two different types of subunits: alpha and beta.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000052297.

Structurei

3D structure databases

ProteinModelPortaliQ8BMN3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3645. Eukaryota.
ENOG410XQGR. LUCA.
GeneTreeiENSGT00760000118930.
HOGENOMiHOG000006756.
HOVERGENiHBG003756.
InParanoidiQ8BMN3.
KOiK04814.
OMAiDHKLSWN.
OrthoDBiEOG091G0R20.
PhylomeDBiQ8BMN3.
TreeFamiTF315605.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BMN3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTGFLRVFLA LSATLSGSWV TLTATAGLSS VAEHEDALLR HLFQGYQKCV
60 70 80 90 100
RPVLNSSDII KVYFGLKISQ LVDVDEKNQL MTTNVWLKQE WTDQKLRWNP
110 120 130 140 150
EDYGGINSIK VPSESLWLPD IVLFENADGR FEGSLMTKAI VKSSGTVSWT
160 170 180 190 200
PPASYKSSCT MDVTFFPFDK QNCSMKFGSW TYDGTMVDLI LINENVDRKD
210 220 230 240 250
FFDNGEWEIL NAKGMKGNRR EGFYSYPFVT YSFVLRRLPL FYTLFLIIPC
260 270 280 290 300
LGLSFLTVLV FYLPSDEGEK LSLSTSVLVS LTVFLLVIEE IIPSSSKVIP
310 320 330 340 350
LIGEYLLFIM IFVTLSIIVT VFVINVHHRS SSTYHPMAPW VKRLFLEKLP
360 370 380 390 400
RWLCMKDPRD RFSFPDGTES KGTVRGKFPG KKKQTPTSDG ERVLVAFLEK
410 420 430 440 450
ASESIRYISR HVKKEHFISQ VVQDWKFVAQ VLDRIFLWLF LTASVLGSVL
460
IFIPALKMWI HRFH
Length:464
Mass (Da):53,112
Last modified:March 1, 2003 - v1
Checksum:i2ECEA3E0DAF2D5EB
GO
Isoform 2 (identifier: Q8BMN3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     75-89: Missing.

Note: No experimental confirmation available.
Show »
Length:449
Mass (Da):51,282
Checksum:iFF54824C5DAAC8E7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti170K → R in AAL75573 (Ref. 1) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01377575 – 89Missing in isoform 2. 1 PublicationAdd BLAST15

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF467896 mRNA. Translation: AAL75573.1.
AY574268 mRNA. Translation: AAS90364.1.
AK017571 mRNA. Translation: BAB30812.1.
AK030464 mRNA. Translation: BAC26973.1.
BC058193 mRNA. Translation: AAH58193.1.
CCDSiCCDS22216.1. [Q8BMN3-2]
CCDS22217.1. [Q8BMN3-1]
RefSeqiNP_081730.1. NM_027454.4. [Q8BMN3-2]
NP_775304.1. NM_173212.4. [Q8BMN3-1]
UniGeneiMm.110444.

Genome annotation databases

EnsembliENSMUST00000060943; ENSMUSP00000052297; ENSMUSG00000031492. [Q8BMN3-1]
ENSMUST00000079463; ENSMUSP00000078428; ENSMUSG00000031492. [Q8BMN3-2]
GeneIDi108043.
KEGGimmu:108043.
UCSCiuc009lit.2. mouse. [Q8BMN3-1]
uc009liu.2. mouse. [Q8BMN3-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF467896 mRNA. Translation: AAL75573.1.
AY574268 mRNA. Translation: AAS90364.1.
AK017571 mRNA. Translation: BAB30812.1.
AK030464 mRNA. Translation: BAC26973.1.
BC058193 mRNA. Translation: AAH58193.1.
CCDSiCCDS22216.1. [Q8BMN3-2]
CCDS22217.1. [Q8BMN3-1]
RefSeqiNP_081730.1. NM_027454.4. [Q8BMN3-2]
NP_775304.1. NM_173212.4. [Q8BMN3-1]
UniGeneiMm.110444.

3D structure databases

ProteinModelPortaliQ8BMN3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000052297.

Chemistry databases

ChEMBLiCHEMBL3350222.

PTM databases

iPTMnetiQ8BMN3.
PhosphoSitePlusiQ8BMN3.

Proteomic databases

PaxDbiQ8BMN3.
PRIDEiQ8BMN3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000060943; ENSMUSP00000052297; ENSMUSG00000031492. [Q8BMN3-1]
ENSMUST00000079463; ENSMUSP00000078428; ENSMUSG00000031492. [Q8BMN3-2]
GeneIDi108043.
KEGGimmu:108043.
UCSCiuc009lit.2. mouse. [Q8BMN3-1]
uc009liu.2. mouse. [Q8BMN3-2]

Organism-specific databases

CTDi1142.
MGIiMGI:106212. Chrnb3.

Phylogenomic databases

eggNOGiKOG3645. Eukaryota.
ENOG410XQGR. LUCA.
GeneTreeiENSGT00760000118930.
HOGENOMiHOG000006756.
HOVERGENiHBG003756.
InParanoidiQ8BMN3.
KOiK04814.
OMAiDHKLSWN.
OrthoDBiEOG091G0R20.
PhylomeDBiQ8BMN3.
TreeFamiTF315605.

Enzyme and pathway databases

ReactomeiR-MMU-629594. Highly calcium permeable postsynaptic nicotinic acetylcholine receptors.
R-MMU-629597. Highly calcium permeable nicotinic acetylcholine receptors.

Miscellaneous databases

PROiQ8BMN3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031492.
ExpressionAtlasiQ8BMN3. baseline and differential.
GenevisibleiQ8BMN3. MM.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACHB3_MOUSE
AccessioniPrimary (citable) accession number: Q8BMN3
Secondary accession number(s): Q8R5H3, Q9CYK8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2005
Last sequence update: March 1, 2003
Last modified: November 30, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.