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Q8BMN3 (ACHB3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neuronal acetylcholine receptor subunit beta-3
Gene names
Name:Chrnb3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length464 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane By similarity.

Subunit structure

Neuronal AChR seems to be composed of two different types of subunits: alpha and beta By similarity.

Subcellular location

Cell junctionsynapsepostsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta-3/CHRNB3 sub-subfamily. [View classification]

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8BMN3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8BMN3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     75-89: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3030 Potential
Chain31 – 464434Neuronal acetylcholine receptor subunit beta-3
PRO_0000000384

Regions

Topological domain31 – 238208Extracellular Potential
Transmembrane239 – 26325Helical; Potential
Transmembrane271 – 28818Helical; Potential
Transmembrane305 – 32622Helical; Potential
Topological domain327 – 434108Cytoplasmic Potential
Transmembrane435 – 45319Helical; Potential

Amino acid modifications

Glycosylation551N-linked (GlcNAc...) Potential
Glycosylation1721N-linked (GlcNAc...) Potential
Disulfide bond159 ↔ 173 By similarity

Natural variations

Alternative sequence75 – 8915Missing in isoform 2.
VSP_013775

Experimental info

Sequence conflict1701K → R in AAL75573. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 2ECEA3E0DAF2D5EB

FASTA46453,112
        10         20         30         40         50         60 
MTGFLRVFLA LSATLSGSWV TLTATAGLSS VAEHEDALLR HLFQGYQKCV RPVLNSSDII 

        70         80         90        100        110        120 
KVYFGLKISQ LVDVDEKNQL MTTNVWLKQE WTDQKLRWNP EDYGGINSIK VPSESLWLPD 

       130        140        150        160        170        180 
IVLFENADGR FEGSLMTKAI VKSSGTVSWT PPASYKSSCT MDVTFFPFDK QNCSMKFGSW 

       190        200        210        220        230        240 
TYDGTMVDLI LINENVDRKD FFDNGEWEIL NAKGMKGNRR EGFYSYPFVT YSFVLRRLPL 

       250        260        270        280        290        300 
FYTLFLIIPC LGLSFLTVLV FYLPSDEGEK LSLSTSVLVS LTVFLLVIEE IIPSSSKVIP 

       310        320        330        340        350        360 
LIGEYLLFIM IFVTLSIIVT VFVINVHHRS SSTYHPMAPW VKRLFLEKLP RWLCMKDPRD 

       370        380        390        400        410        420 
RFSFPDGTES KGTVRGKFPG KKKQTPTSDG ERVLVAFLEK ASESIRYISR HVKKEHFISQ 

       430        440        450        460 
VVQDWKFVAQ VLDRIFLWLF LTASVLGSVL IFIPALKMWI HRFH 

« Hide

Isoform 2 [UniParc].

Checksum: FF54824C5DAAC8E7
Show »

FASTA44951,282

References

« Hide 'large scale' references
[1]Stitzel J.A., Lautner M.A., Jimenez M., Bhandarkar S.J., Curtis C.D., Remias J.
Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]Groot-Kormelink P.J.
Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: BALB/c.
Tissue: Brain.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J.
Tissue: Pituitary.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Eye.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF467896 mRNA. Translation: AAL75573.1.
AY574268 mRNA. Translation: AAS90364.1.
AK017571 mRNA. Translation: BAB30812.1.
AK030464 mRNA. Translation: BAC26973.1.
BC058193 mRNA. Translation: AAH58193.1.
CCDSCCDS22216.1. [Q8BMN3-2]
CCDS22217.1. [Q8BMN3-1]
RefSeqNP_081730.1. NM_027454.4. [Q8BMN3-2]
NP_775304.1. NM_173212.4. [Q8BMN3-1]
UniGeneMm.110444.

3D structure databases

ProteinModelPortalQ8BMN3.
SMRQ8BMN3. Positions 32-346, 401-452.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBQ8BMN3.
GuidetoPHARMACOLOGY473.

PTM databases

PhosphoSiteQ8BMN3.

Proteomic databases

PaxDbQ8BMN3.
PRIDEQ8BMN3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000060943; ENSMUSP00000052297; ENSMUSG00000031492. [Q8BMN3-1]
ENSMUST00000079463; ENSMUSP00000078428; ENSMUSG00000031492. [Q8BMN3-2]
GeneID108043.
KEGGmmu:108043.
UCSCuc009lit.2. mouse. [Q8BMN3-1]
uc009liu.2. mouse. [Q8BMN3-2]

Organism-specific databases

CTD1142.
MGIMGI:106212. Chrnb3.

Phylogenomic databases

eggNOGNOG252943.
GeneTreeENSGT00750000117712.
HOGENOMHOG000006756.
HOVERGENHBG003756.
InParanoidQ8BMN3.
KOK04814.
OMADRYSFPD.
OrthoDBEOG72JWGV.
PhylomeDBQ8BMN3.
TreeFamTF315605.

Gene expression databases

BgeeQ8BMN3.
GenevestigatorQ8BMN3.

Family and domain databases

Gene3D1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERPTHR18945. PTHR18945. 1 hit.
PfamPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 2 hits.
[Graphical view]
PRINTSPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsTIGR00860. LIC. 1 hit.
PROSITEPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCHRNB3. mouse.
NextBio359931.
PROQ8BMN3.
SOURCESearch...

Entry information

Entry nameACHB3_MOUSE
AccessionPrimary (citable) accession number: Q8BMN3
Secondary accession number(s): Q8R5H3, Q9CYK8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 24, 2005
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot