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Protein

Protein-methionine sulfoxide oxidase MICAL2

Gene

Mical2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Nuclear monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin and regulates the SRF signaling. Acts by modifying nuclear actin subunits through the addition of oxygen to form methionine-sulfoxide, leading to promote actin filament severing and prevent repolymerization. Acts as a key regulator of the SRF signaling pathway elicited by nerve growth factor and serum: mediates oxidation and subsequent depolymerization of nuclear actin, leading to increase MKL1/MRTF-A presence in the nucleus and promote SRF:MKL1/MRTF-A-dependent gene transcription. Does not activate SRF:MKL1/MRTF-A through RhoA.1 Publication

Catalytic activityi

[protein]-methionine + NADPH + O2 = [protein]-methionine-sulfoxide + NADP+ + H2O.1 Publication

Cofactori

FADBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei97 – 971FADBy similarity
Binding sitei116 – 1161FADBy similarity
Binding sitei118 – 1181FADBy similarity
Binding sitei123 – 1231FADBy similarity
Binding sitei125 – 1251FADBy similarity
Binding sitei398 – 3981FADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi97 – 12529FADBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Actin-binding, FAD, Flavoprotein, Metal-binding, NADP, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-methionine sulfoxide oxidase MICAL2 (EC:1.14.13.-)
Alternative name(s):
Molecule interacting with CasL protein 2
Short name:
MICAL-2
Short name:
mMical2
Gene namesi
Name:Mical2
Synonyms:Kiaa0750
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2444947. Mical2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 960960Protein-methionine sulfoxide oxidase MICAL2PRO_0000075845Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Isoform 2 (identifier: Q8BML1-2)
Modified residuei812 – 8121PhosphoserineCombined sources
Modified residuei1083 – 10831PhosphothreonineCombined sources
Modified residuei1087 – 10871PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8BML1.
PaxDbiQ8BML1.
PRIDEiQ8BML1.

PTM databases

iPTMnetiQ8BML1.
PhosphoSiteiQ8BML1.

Expressioni

Gene expression databases

BgeeiQ8BML1.
ExpressionAtlasiQ8BML1. baseline and differential.
GenevisibleiQ8BML1. MM.

Interactioni

Subunit structurei

Interacts with RAB1B (By similarity). Interacts with VIM and PLXNA4.By similarity1 Publication

GO - Molecular functioni

  • actin binding Source: UniProtKB

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000047639.

Structurei

3D structure databases

ProteinModelPortaliQ8BML1.
SMRiQ8BML1. Positions 16-488, 511-629, 749-807.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini516 – 619104CHPROSITE-ProRule annotationAdd
BLAST
Domaini751 – 81363LIM zinc-bindingPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 494493Monooxygenase domainBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi660 – 68122Nuclear localization signalBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the Mical family.Curated
Contains 1 CH (calponin-homology) domain.PROSITE-ProRule annotation
Contains 1 LIM zinc-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

LIM domain

Phylogenomic databases

eggNOGiKOG1700. Eukaryota.
COG5069. LUCA.
GeneTreeiENSGT00760000118856.
HOGENOMiHOG000047263.
HOVERGENiHBG052474.
InParanoidiQ8BML1.
KOiK19947.
OMAiDHRPKAQ.
OrthoDBiEOG769ZHM.
TreeFamiTF324129.

Family and domain databases

Gene3Di1.10.418.10. 1 hit.
2.10.110.10. 1 hit.
3.50.50.60. 1 hit.
InterProiIPR001715. CH-domain.
IPR002938. FAD-bd.
IPR023753. FAD/NAD-binding_dom.
IPR001781. Znf_LIM.
[Graphical view]
PfamiPF00307. CH. 1 hit.
PF01494. FAD_binding_3. 1 hit.
PF00412. LIM. 1 hit.
[Graphical view]
SMARTiSM00033. CH. 1 hit.
SM00132. LIM. 1 hit.
[Graphical view]
SUPFAMiSSF47576. SSF47576. 1 hit.
SSF51905. SSF51905. 2 hits.
PROSITEiPS50021. CH. 1 hit.
PS00478. LIM_DOMAIN_1. 1 hit.
PS50023. LIM_DOMAIN_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BML1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGENEDEKQA QASQVFENFV QATTCKGTLQ AFNILTCLLD LDPLDHRNFY
60 70 80 90 100
SQLKSKVNTW KAKALWHKLD KRGSHKEYKR GKACSNTKCL IVGGGPCGLR
110 120 130 140 150
TAIELAYLGA KVVVVEKRDT FSRNNVLHLW PFTIHDLRGL GAKKFYGKFC
160 170 180 190 200
AGSIDHISIR QLQLILFKVA LMLGVEVHVN VEFVRVLEPP EDQENQKVGW
210 220 230 240 250
RAEFLPADHA LSDFEFDVII GADGHRNTLE GFRRKEFRGK LAIAITANFI
260 270 280 290 300
NRNSTAEAKV EEISGVAFIF NQKFFQDLKE ETGIDLENIV YYKDSTHYFV
310 320 330 340 350
MTAKKQSLLD KGVILNDYID TEMLLCSENV NQDNLLSYAR EAADFATNYQ
360 370 380 390 400
LPSLDFAINH NGQPDVAMFD FTSMYASENA ALMRERQAHQ LLVALVGDSL
410 420 430 440 450
LEPFWPMGTG CARGFLAAFD TAWMVKSWDQ GTPPLEVLAE RESLYRLLPQ
460 470 480 490 500
TTPENINKNF EQYTLDPATR YPNLNLHCVR PHQVKHLYIT KEMDRFPLER
510 520 530 540 550
WGSVRRSVSL SRRESDIRPN KLLTWCQQQT KGYQHVRVTD LTTSWRSGLA
560 570 580 590 600
LCAIIHSFRP ELINFDSLNE DDAVENNQLA FDVAKREFGI LPVTTGKEMA
610 620 630 640 650
STQEPDKLSM VMYLSKFYEL FRGTPLRPMD SWRKNYGENA DFGLGKTFIQ
660 670 680 690 700
NNYLNLTLPR KRTPRVDTQT EENDMNKRRR QGFNHLEELP SFSSRSLGSS
710 720 730 740 750
QEYAKESGSQ NKVKHMANQL LAKFEENTRN PSVVKQESPR KAFPLSLGGR
760 770 780 790 800
DTCYFCKKRV YMIERLSAEG HFFHQECFRC SVCSATLRLA AYAFDCDEGK
810 820 830 840 850
FYCKPHFVHC KTSSKQRKRR AELNQQREEE GTWQEQEAPR RDVPTESSCA
860 870 880 890 900
VAAISTPEGS PPGTSTSFFR KALSWPLRLT RGLLNLPQSL LRWMQGLQEA
910 920 930 940 950
AGHHVRDNAH NYCFMFELLS LGLLLLWAFS KVLAAMYRES EESLENIRSW
960
LLRFIPVKLQ
Length:960
Mass (Da):110,072
Last modified:March 1, 2003 - v1
Checksum:i0D70BBA2144730E2
GO
Isoform 2 (identifier: Q8BML1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     736-736: Q → QDCRRVSGIG...PKTQATSPDL
     863-960: GTSTSFFRKA...LLRFIPVKLQ → VRFSLPVLHPLLG

Note: No experimental confirmation available.Combined sources
Show »
Length:1,102
Mass (Da):124,257
Checksum:iF87D341BF3BA9E46
GO
Isoform 3 (identifier: Q8BML1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     799-960: GKFYCKPHFV...LLRFIPVKLQ → GNPRGQESTSLWPWSQM

Note: No experimental confirmation available.
Show »
Length:815
Mass (Da):93,331
Checksum:iAD9FB09B5ED58458
GO

Sequence cautioni

The sequence BAD90416.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei736 – 7361Q → QDCRRVSGIGKPVLCSASRP PGTSCCPKLEESTPRLPPPL KRQFSSTVATGQVLRELNQV PASGECPSRPWRARAKSDLQ LGGVENLATLPRTCQGALAL SGVLRRLQQVEEKVLQKRAQ NLANREFHTKNIKEKAAHLA SMFGHGDLPQDKLLSKRVPH AHPPSPPSCLPSPHPAAASS PPAADSVSPARKLTVGKVSS GIGAAAEVLVNLYLNDHRPK TQATSPDL in isoform 2. 1 PublicationVSP_042597
Alternative sequencei799 – 960162GKFYC…PVKLQ → GNPRGQESTSLWPWSQM in isoform 3. 1 PublicationVSP_042598Add
BLAST
Alternative sequencei863 – 96098GTSTS…PVKLQ → VRFSLPVLHPLLG in isoform 2. 1 PublicationVSP_042599Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK220353 mRNA. Translation: BAD90416.1. Different initiation.
AK030608 mRNA. Translation: BAC27044.1.
AK138935 mRNA. Translation: BAE23824.1.
AK143191 mRNA. Translation: BAE25298.1.
AK163078 mRNA. Translation: BAE37182.1.
AC100153 Genomic DNA. No translation available.
AC132392 Genomic DNA. No translation available.
AC166834 Genomic DNA. No translation available.
BC111895 mRNA. Translation: AAI11896.1.
BC112415 mRNA. Translation: AAI12416.1.
CCDSiCCDS21753.1. [Q8BML1-1]
CCDS57581.1. [Q8BML1-2]
RefSeqiNP_001180234.1. NM_001193305.1. [Q8BML1-2]
NP_796256.1. NM_177282.5. [Q8BML1-1]
XP_006508017.1. XM_006507954.1. [Q8BML1-2]
UniGeneiMm.488195.
Mm.52312.

Genome annotation databases

EnsembliENSMUST00000037991; ENSMUSP00000047639; ENSMUSG00000038244. [Q8BML1-2]
ENSMUST00000050149; ENSMUSP00000051163; ENSMUSG00000038244. [Q8BML1-1]
ENSMUST00000106648; ENSMUSP00000102259; ENSMUSG00000038244. [Q8BML1-3]
GeneIDi320878.
KEGGimmu:320878.
UCSCiuc009jgm.2. mouse. [Q8BML1-3]
uc009jgn.2. mouse. [Q8BML1-1]
uc009jgo.2. mouse. [Q8BML1-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK220353 mRNA. Translation: BAD90416.1. Different initiation.
AK030608 mRNA. Translation: BAC27044.1.
AK138935 mRNA. Translation: BAE23824.1.
AK143191 mRNA. Translation: BAE25298.1.
AK163078 mRNA. Translation: BAE37182.1.
AC100153 Genomic DNA. No translation available.
AC132392 Genomic DNA. No translation available.
AC166834 Genomic DNA. No translation available.
BC111895 mRNA. Translation: AAI11896.1.
BC112415 mRNA. Translation: AAI12416.1.
CCDSiCCDS21753.1. [Q8BML1-1]
CCDS57581.1. [Q8BML1-2]
RefSeqiNP_001180234.1. NM_001193305.1. [Q8BML1-2]
NP_796256.1. NM_177282.5. [Q8BML1-1]
XP_006508017.1. XM_006507954.1. [Q8BML1-2]
UniGeneiMm.488195.
Mm.52312.

3D structure databases

ProteinModelPortaliQ8BML1.
SMRiQ8BML1. Positions 16-488, 511-629, 749-807.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000047639.

PTM databases

iPTMnetiQ8BML1.
PhosphoSiteiQ8BML1.

Proteomic databases

MaxQBiQ8BML1.
PaxDbiQ8BML1.
PRIDEiQ8BML1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000037991; ENSMUSP00000047639; ENSMUSG00000038244. [Q8BML1-2]
ENSMUST00000050149; ENSMUSP00000051163; ENSMUSG00000038244. [Q8BML1-1]
ENSMUST00000106648; ENSMUSP00000102259; ENSMUSG00000038244. [Q8BML1-3]
GeneIDi320878.
KEGGimmu:320878.
UCSCiuc009jgm.2. mouse. [Q8BML1-3]
uc009jgn.2. mouse. [Q8BML1-1]
uc009jgo.2. mouse. [Q8BML1-2]

Organism-specific databases

CTDi9645.
MGIiMGI:2444947. Mical2.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1700. Eukaryota.
COG5069. LUCA.
GeneTreeiENSGT00760000118856.
HOGENOMiHOG000047263.
HOVERGENiHBG052474.
InParanoidiQ8BML1.
KOiK19947.
OMAiDHRPKAQ.
OrthoDBiEOG769ZHM.
TreeFamiTF324129.

Miscellaneous databases

ChiTaRSiMical2. mouse.
NextBioi397627.
PROiQ8BML1.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BML1.
ExpressionAtlasiQ8BML1. baseline and differential.
GenevisibleiQ8BML1. MM.

Family and domain databases

Gene3Di1.10.418.10. 1 hit.
2.10.110.10. 1 hit.
3.50.50.60. 1 hit.
InterProiIPR001715. CH-domain.
IPR002938. FAD-bd.
IPR023753. FAD/NAD-binding_dom.
IPR001781. Znf_LIM.
[Graphical view]
PfamiPF00307. CH. 1 hit.
PF01494. FAD_binding_3. 1 hit.
PF00412. LIM. 1 hit.
[Graphical view]
SMARTiSM00033. CH. 1 hit.
SM00132. LIM. 1 hit.
[Graphical view]
SUPFAMiSSF47576. SSF47576. 1 hit.
SSF51905. SSF51905. 2 hits.
PROSITEiPS50021. CH. 1 hit.
PS00478. LIM_DOMAIN_1. 1 hit.
PS50023. LIM_DOMAIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
    Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
    Strain: C57BL/6J.
    Tissue: Aorta, Eye, Pituitary and Vein.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  5. "MICALs, a family of conserved flavoprotein oxidoreductases, function in plexin-mediated axonal repulsion."
    Terman J.R., Mao T., Pasterkamp R.J., Yu H.-H., Kolodkin A.L.
    Cell 109:887-900(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PLXNA4.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-812; THR-1083 AND SER-1087 (ISOFORM 2), IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Heart.
  7. "MsrB1 and MICALs regulate actin assembly and macrophage function via reversible stereoselective methionine oxidation."
    Lee B.C., Peterfi Z., Hoffmann F.W., Moore R.E., Kaya A., Avanesov A., Tarrago L., Zhou Y., Weerapana E., Fomenko D.E., Hoffmann P.R., Gladyshev V.N.
    Mol. Cell 51:397-404(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.

Entry informationi

Entry nameiMICA2_MOUSE
AccessioniPrimary (citable) accession number: Q8BML1
Secondary accession number(s): F6ZD54
, F8WJF1, Q14DP1, Q3TR48, Q3UPU6, Q5DU17
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: March 1, 2003
Last modified: May 11, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.