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Q8BMJ8

- SP8_MOUSE

UniProt

Q8BMJ8 - SP8_MOUSE

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Protein

Transcription factor Sp8

Gene

Sp8

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Transcription factor which plays a key role in limb development. Positively regulates FGF8 expression in the apical ectodermal ridge (AER) and contributes to limb outgrowth in embryos.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri356 – 38025C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri386 – 41025C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri416 – 43823C2H2-type 3PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. dorsal/ventral pattern formation Source: MGI
  2. embryonic limb morphogenesis Source: UniProtKB
  3. proximal/distal pattern formation Source: MGI
  4. regulation of transcription, DNA-templated Source: UniProtKB-KW
  5. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor Sp8
Gene namesi
Name:Sp8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 12

Organism-specific databases

MGIiMGI:2443471. Sp8.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 486486Transcription factor Sp8PRO_0000047153Add
BLAST

Proteomic databases

PRIDEiQ8BMJ8.

PTM databases

PhosphoSiteiQ8BMJ8.

Expressioni

Developmental stagei

Expression is detected in the forebrain, midbrain/hindbrain boundary and neural tube. At the stage of limb bud outgrowth, expressed in a scattered manner in the ventral ectoderm, and is later confined to the apical ectodermal ridge (AER).1 Publication

Gene expression databases

BgeeiQ8BMJ8.
CleanExiMM_SP8.
ExpressionAtlasiQ8BMJ8. baseline and differential.
GenevestigatoriQ8BMJ8.

Structurei

3D structure databases

ProteinModelPortaliQ8BMJ8.
SMRiQ8BMJ8. Positions 324-472.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi11 – 10494Ser-richAdd
BLAST
Compositional biasi68 – 11649Ala-richAdd
BLAST
Compositional biasi132 – 14918Gly-richAdd
BLAST

Sequence similaritiesi

Contains 3 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri356 – 38025C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri386 – 41025C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri416 – 43823C2H2-type 3PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00760000118984.
HOGENOMiHOG000231067.
HOVERGENiHBG036899.
InParanoidiQ8BMJ8.
KOiK09198.
OMAiXHSCHIP.
OrthoDBiEOG7JQBMX.
PhylomeDBiQ8BMJ8.
TreeFamiTF350150.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BMJ8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLAATCNKIG SPSPSPSSLS DSSSSFGKGF HPWKRSSSSS SGSCNVVGSS
60 70 80 90 100
LSSFGVSGAS RNGGSSSAAA AAAAAAAAAA ALVSDSFSCG GSPGSSAFSL
110 120 130 140 150
TSSSAAAAAA AAAAAASSSP FANDYSVFQA PGVSGGSGGG GGGGGGGSGA
160 170 180 190 200
HSQDSSHQPV FISKVHTSVD GLQGIYPRVG MAHPYESWFK PSHPGLGAAA
210 220 230 240 250
DVGSAGASSW WDVGAGWIDV QNPNGAAALP GSLHPAAGGL QTSLHSPLGG
260 270 280 290 300
YNSDYSGLSH SAFSSGASSH LLSPAGQHLM DGFKPVLPGS YPDSAPSPLA
310 320 330 340 350
GAGSSMLSAG PAAQLGGSPR SSARRYSGRA TCDCPNCQEA ERLGPAGASL
360 370 380 390 400
RRKGLHSCHI PGCGKVYGKT SHLKAHLRWH TGERPFVCNW LFCGKRFTRS
410 420 430 440 450
DELQRHLRTH TGEKRFACPV CNKRFMRSDH LSKHVKTHSG GGGSAGSGGG
460 470 480
KKGSDTDSEH SAAGSPPCHS PELLQPPEPG HRNGLE
Length:486
Mass (Da):48,417
Last modified:March 1, 2003 - v1
Checksum:i336C36B548646883
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK030745 mRNA. Translation: BAC27113.1.
AK053093 mRNA. Translation: BAC35264.1.
CCDSiCCDS26213.1.
RefSeqiNP_796056.2. NM_177082.4.
XP_006516046.1. XM_006515983.1.
XP_006516047.1. XM_006515984.1.
XP_006516048.1. XM_006515985.1.
XP_006516049.1. XM_006515986.1.
XP_006516050.1. XM_006515987.1.
XP_006516051.1. XM_006515988.1.
UniGeneiMm.51682.

Genome annotation databases

EnsembliENSMUST00000063918; ENSMUSP00000065746; ENSMUSG00000048562.
GeneIDi320145.
KEGGimmu:320145.
UCSCiuc007pig.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK030745 mRNA. Translation: BAC27113.1 .
AK053093 mRNA. Translation: BAC35264.1 .
CCDSi CCDS26213.1.
RefSeqi NP_796056.2. NM_177082.4.
XP_006516046.1. XM_006515983.1.
XP_006516047.1. XM_006515984.1.
XP_006516048.1. XM_006515985.1.
XP_006516049.1. XM_006515986.1.
XP_006516050.1. XM_006515987.1.
XP_006516051.1. XM_006515988.1.
UniGenei Mm.51682.

3D structure databases

ProteinModelPortali Q8BMJ8.
SMRi Q8BMJ8. Positions 324-472.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q8BMJ8.

Proteomic databases

PRIDEi Q8BMJ8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000063918 ; ENSMUSP00000065746 ; ENSMUSG00000048562 .
GeneIDi 320145.
KEGGi mmu:320145.
UCSCi uc007pig.2. mouse.

Organism-specific databases

CTDi 221833.
MGIi MGI:2443471. Sp8.

Phylogenomic databases

eggNOGi COG5048.
GeneTreei ENSGT00760000118984.
HOGENOMi HOG000231067.
HOVERGENi HBG036899.
InParanoidi Q8BMJ8.
KOi K09198.
OMAi XHSCHIP.
OrthoDBi EOG7JQBMX.
PhylomeDBi Q8BMJ8.
TreeFami TF350150.

Miscellaneous databases

NextBioi 396123.
PROi Q8BMJ8.
SOURCEi Search...

Gene expression databases

Bgeei Q8BMJ8.
CleanExi MM_SP8.
ExpressionAtlasi Q8BMJ8. baseline and differential.
Genevestigatori Q8BMJ8.

Family and domain databases

Gene3Di 3.30.160.60. 3 hits.
InterProi IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view ]
SMARTi SM00355. ZnF_C2H2. 3 hits.
[Graphical view ]
PROSITEi PS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Head.
  2. "Sp8 and Sp9, two closely related buttonhead-like transcription factors, regulate Fgf8 expression and limb outgrowth in vertebrate embryos."
    Kawakami Y., Rodriguez Esteban C., Matsui T., Rodriguez-Leon J., Kato S., Izpisua Belmonte J.C.
    Development 131:4763-4774(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiSP8_MOUSE
AccessioniPrimary (citable) accession number: Q8BMJ8
Secondary accession number(s): Q8BWA3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: March 1, 2003
Last modified: October 29, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3