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Q8BMJ8

- SP8_MOUSE

UniProt

Q8BMJ8 - SP8_MOUSE

Protein

Transcription factor Sp8

Gene

Sp8

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Transcription factor which plays a key role in limb development. Positively regulates FGF8 expression in the apical ectodermal ridge (AER) and contributes to limb outgrowth in embryos.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri356 – 38025C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri386 – 41025C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri416 – 43823C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: MGI
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. dorsal/ventral pattern formation Source: MGI
    2. embryonic limb morphogenesis Source: UniProtKB
    3. proximal/distal pattern formation Source: MGI
    4. regulation of transcription, DNA-templated Source: UniProtKB-KW
    5. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription factor Sp8
    Gene namesi
    Name:Sp8
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 12

    Organism-specific databases

    MGIiMGI:2443471. Sp8.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 486486Transcription factor Sp8PRO_0000047153Add
    BLAST

    Proteomic databases

    PRIDEiQ8BMJ8.

    PTM databases

    PhosphoSiteiQ8BMJ8.

    Expressioni

    Developmental stagei

    Expression is detected in the forebrain, midbrain/hindbrain boundary and neural tube. At the stage of limb bud outgrowth, expressed in a scattered manner in the ventral ectoderm, and is later confined to the apical ectodermal ridge (AER).1 Publication

    Gene expression databases

    BgeeiQ8BMJ8.
    CleanExiMM_SP8.
    GenevestigatoriQ8BMJ8.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BMJ8.
    SMRiQ8BMJ8. Positions 324-472.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi11 – 10494Ser-richAdd
    BLAST
    Compositional biasi68 – 11649Ala-richAdd
    BLAST
    Compositional biasi132 – 14918Gly-richAdd
    BLAST

    Sequence similaritiesi

    Contains 3 C2H2-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri356 – 38025C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri386 – 41025C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri416 – 43823C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5048.
    GeneTreeiENSGT00750000117314.
    HOGENOMiHOG000231067.
    HOVERGENiHBG036899.
    InParanoidiQ8BMJ8.
    KOiK09198.
    OMAiXHSCHIP.
    OrthoDBiEOG7JQBMX.
    PhylomeDBiQ8BMJ8.
    TreeFamiTF350150.

    Family and domain databases

    Gene3Di3.30.160.60. 3 hits.
    InterProiIPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view]
    SMARTiSM00355. ZnF_C2H2. 3 hits.
    [Graphical view]
    PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
    PS50157. ZINC_FINGER_C2H2_2. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8BMJ8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLAATCNKIG SPSPSPSSLS DSSSSFGKGF HPWKRSSSSS SGSCNVVGSS    50
    LSSFGVSGAS RNGGSSSAAA AAAAAAAAAA ALVSDSFSCG GSPGSSAFSL 100
    TSSSAAAAAA AAAAAASSSP FANDYSVFQA PGVSGGSGGG GGGGGGGSGA 150
    HSQDSSHQPV FISKVHTSVD GLQGIYPRVG MAHPYESWFK PSHPGLGAAA 200
    DVGSAGASSW WDVGAGWIDV QNPNGAAALP GSLHPAAGGL QTSLHSPLGG 250
    YNSDYSGLSH SAFSSGASSH LLSPAGQHLM DGFKPVLPGS YPDSAPSPLA 300
    GAGSSMLSAG PAAQLGGSPR SSARRYSGRA TCDCPNCQEA ERLGPAGASL 350
    RRKGLHSCHI PGCGKVYGKT SHLKAHLRWH TGERPFVCNW LFCGKRFTRS 400
    DELQRHLRTH TGEKRFACPV CNKRFMRSDH LSKHVKTHSG GGGSAGSGGG 450
    KKGSDTDSEH SAAGSPPCHS PELLQPPEPG HRNGLE 486
    Length:486
    Mass (Da):48,417
    Last modified:March 1, 2003 - v1
    Checksum:i336C36B548646883
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK030745 mRNA. Translation: BAC27113.1.
    AK053093 mRNA. Translation: BAC35264.1.
    CCDSiCCDS26213.1.
    RefSeqiNP_796056.2. NM_177082.4.
    XP_006516046.1. XM_006515983.1.
    XP_006516047.1. XM_006515984.1.
    XP_006516048.1. XM_006515985.1.
    XP_006516049.1. XM_006515986.1.
    XP_006516050.1. XM_006515987.1.
    XP_006516051.1. XM_006515988.1.
    UniGeneiMm.51682.

    Genome annotation databases

    EnsembliENSMUST00000063918; ENSMUSP00000065746; ENSMUSG00000048562.
    GeneIDi320145.
    KEGGimmu:320145.
    UCSCiuc007pig.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK030745 mRNA. Translation: BAC27113.1 .
    AK053093 mRNA. Translation: BAC35264.1 .
    CCDSi CCDS26213.1.
    RefSeqi NP_796056.2. NM_177082.4.
    XP_006516046.1. XM_006515983.1.
    XP_006516047.1. XM_006515984.1.
    XP_006516048.1. XM_006515985.1.
    XP_006516049.1. XM_006515986.1.
    XP_006516050.1. XM_006515987.1.
    XP_006516051.1. XM_006515988.1.
    UniGenei Mm.51682.

    3D structure databases

    ProteinModelPortali Q8BMJ8.
    SMRi Q8BMJ8. Positions 324-472.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q8BMJ8.

    Proteomic databases

    PRIDEi Q8BMJ8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000063918 ; ENSMUSP00000065746 ; ENSMUSG00000048562 .
    GeneIDi 320145.
    KEGGi mmu:320145.
    UCSCi uc007pig.2. mouse.

    Organism-specific databases

    CTDi 221833.
    MGIi MGI:2443471. Sp8.

    Phylogenomic databases

    eggNOGi COG5048.
    GeneTreei ENSGT00750000117314.
    HOGENOMi HOG000231067.
    HOVERGENi HBG036899.
    InParanoidi Q8BMJ8.
    KOi K09198.
    OMAi XHSCHIP.
    OrthoDBi EOG7JQBMX.
    PhylomeDBi Q8BMJ8.
    TreeFami TF350150.

    Miscellaneous databases

    NextBioi 396123.
    PROi Q8BMJ8.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8BMJ8.
    CleanExi MM_SP8.
    Genevestigatori Q8BMJ8.

    Family and domain databases

    Gene3Di 3.30.160.60. 3 hits.
    InterProi IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view ]
    SMARTi SM00355. ZnF_C2H2. 3 hits.
    [Graphical view ]
    PROSITEi PS00028. ZINC_FINGER_C2H2_1. 3 hits.
    PS50157. ZINC_FINGER_C2H2_2. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Head.
    2. "Sp8 and Sp9, two closely related buttonhead-like transcription factors, regulate Fgf8 expression and limb outgrowth in vertebrate embryos."
      Kawakami Y., Rodriguez Esteban C., Matsui T., Rodriguez-Leon J., Kato S., Izpisua Belmonte J.C.
      Development 131:4763-4774(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiSP8_MOUSE
    AccessioniPrimary (citable) accession number: Q8BMJ8
    Secondary accession number(s): Q8BWA3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 16, 2003
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3