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Protein

Leucine--tRNA ligase, cytoplasmic

Gene

Lars

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei721 – 7211ATPBy similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. leucine-tRNA ligase activity Source: GO_Central

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligase, cytoplasmic (EC:6.1.1.4)
Alternative name(s):
Leucyl-tRNA synthetase
Short name:
LeuRS
Gene namesi
Name:Lars
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 18

Organism-specific databases

MGIiMGI:1913808. Lars.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. cytosol Source: GO_Central
  3. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11781178Leucine--tRNA ligase, cytoplasmicPRO_0000152151Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei972 – 9721N6-acetyllysine1 Publication
Modified residuei1049 – 10491N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ8BMJ2.
PaxDbiQ8BMJ2.
PRIDEiQ8BMJ2.

PTM databases

PhosphoSiteiQ8BMJ2.

Expressioni

Gene expression databases

BgeeiQ8BMJ2.
CleanExiMM_LARS.
ExpressionAtlasiQ8BMJ2. baseline and differential.
GenevestigatoriQ8BMJ2.

Interactioni

Protein-protein interaction databases

BioGridi223191. 3 interactions.
IntActiQ8BMJ2. 1 interaction.
MINTiMINT-1848936.

Structurei

3D structure databases

ProteinModelPortaliQ8BMJ2.
SMRiQ8BMJ2. Positions 13-930.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi55 – 6511"HIGH" regionAdd
BLAST
Motifi718 – 7225"KMSKS" region

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0495.
GeneTreeiENSGT00390000012163.
HOVERGENiHBG055325.
InParanoidiQ8BMJ2.
KOiK01869.
OMAiYEGVMLV.
OrthoDBiEOG7VB2DJ.
PhylomeDBiQ8BMJ2.
TreeFamiTF105718.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BMJ2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAGRKGTAKV DFLKEIEKEA QQKWEAEKVF EVSASRLEKQ KQSSKGKYFV
60 70 80 90 100
TFPYPYMNGR LHLGHTFSLS KCEFAVGYQR LKGKSCLFPF GLHCTGMPIK
110 120 130 140 150
ACADKLKREI ELYGCPPDFP EEEEEEEESS AKPGDIVVRD KAKGKKSKAA
160 170 180 190 200
AKAGSSKYQW DIMKSLGLSD DDIVKFSEAE HWLDYFPPLA VQDLKTIGLK
210 220 230 240 250
VDWRRSFITT DVNPYYDSFV RWQFLTLRER NKIKFGKRYT IYSPKDGQPC
260 270 280 290 300
MDHDRQTGEG VGPQEYTLVK LKVLEPYPSK LSGLKGKNIF LVAATLRPET
310 320 330 340 350
MFGQTNCWVR PDMKYIGFET ANGDIFICTQ RAARNMSYQG FTKHNGVVPV
360 370 380 390 400
VKELMGEEIL GASLSAPLTC YKVVYVLPML TIKEDKGTGV VTSVPSDSPD
410 420 430 440 450
DLAALRDLKK KQALRTKFGI RDDMVLPFEP VPVLEIPGIG NLPAVTVCDE
460 470 480 490 500
LKIQSQNDRE KLAEAKEKLY LRGFYDGVML VDGFKGQKIQ HVKKTIQKNM
510 520 530 540 550
IDAGDALIYM EPEKQVMSRS ADECVVALCD QWYLDYGDEN WKKQTFQCLK
560 570 580 590 600
NMETFCEESR KNFEASLDWL QEHACSRTYG LGTRLPWDEQ WLIESLSDST
610 620 630 640 650
IYMAFYTVAH LLQGGDLNGQ AESPLGIRPQ QMTKDVWDYV FFKDAPFPKT
660 670 680 690 700
QIPKEKLDQL KQEFEFWYPV DLRASGKDLI PNHLSYYIYN HVAMWPEQSD
710 720 730 740 750
KWPVSVRANG HLLLNSEKMS KSTGNFLTLS QAVDKFSADG MRLALADAGD
760 770 780 790 800
TVEDANFVEA MADAGILRLY TWVEWVKEML ASCSSLRSGP ADSFNDRVFA
810 820 830 840 850
SEMNAGIIKT DQNYEKMMFK EALKTGFFEF QAAKDKYREL ATEGMHRELV
860 870 880 890 900
FRFIEVQTIL LTPFCPHLCE HIWTLLGKPD SIMHASWPVA GPVDESLIRS
910 920 930 940 950
SQYLMEVAHD LRLRLKNYMM PAKGKKTDKQ PAQRPSHCTI YVAKNYPVWQ
960 970 980 990 1000
HITLTTLRSH FEANNGKLPD NKVIASELGS LPELKKYMKK VMPFVAMIKE
1010 1020 1030 1040 1050
NMEKKGPRVL DLELEFDEQA VLMENIVYLT NSLELEHIEV KFASEAEDKV
1060 1070 1080 1090 1100
REECCPGKPL NVFRTEPGVP VSLVNPQPSS GHFSTKIDIR QGDSCESIIR
1110 1120 1130 1140 1150
RLMKTDRGIK DLSKVKLMRF DDPLLGPRRV PVLGREHSEK TLISENAVFH
1160 1170
VDLVSKKVHL TENGLRTDIG DTMVYLVH
Length:1,178
Mass (Da):134,192
Last modified:June 7, 2004 - v2
Checksum:i1186583D5237FFC1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti218 – 2181S → P in BAC33766. (PubMed:16141072)Curated
Sequence conflicti869 – 8691C → S in BAC33766. (PubMed:16141072)Curated
Sequence conflicti954 – 9541L → Q in BAC27133. (PubMed:16141072)Curated
Sequence conflicti1127 – 11271P → H in BAC27133. (PubMed:16141072)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030778 mRNA. Translation: BAC27133.1.
AK049472 mRNA. Translation: BAC33766.1.
CCDSiCCDS37796.1.
RefSeqiNP_598898.2. NM_134137.2.
UniGeneiMm.312170.

Genome annotation databases

EnsembliENSMUST00000097590; ENSMUSP00000095197; ENSMUSG00000024493.
GeneIDi107045.
KEGGimmu:107045.
UCSCiuc008etp.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030778 mRNA. Translation: BAC27133.1.
AK049472 mRNA. Translation: BAC33766.1.
CCDSiCCDS37796.1.
RefSeqiNP_598898.2. NM_134137.2.
UniGeneiMm.312170.

3D structure databases

ProteinModelPortaliQ8BMJ2.
SMRiQ8BMJ2. Positions 13-930.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi223191. 3 interactions.
IntActiQ8BMJ2. 1 interaction.
MINTiMINT-1848936.

PTM databases

PhosphoSiteiQ8BMJ2.

Proteomic databases

MaxQBiQ8BMJ2.
PaxDbiQ8BMJ2.
PRIDEiQ8BMJ2.

Protocols and materials databases

DNASUi107045.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000097590; ENSMUSP00000095197; ENSMUSG00000024493.
GeneIDi107045.
KEGGimmu:107045.
UCSCiuc008etp.1. mouse.

Organism-specific databases

CTDi51520.
MGIiMGI:1913808. Lars.

Phylogenomic databases

eggNOGiCOG0495.
GeneTreeiENSGT00390000012163.
HOVERGENiHBG055325.
InParanoidiQ8BMJ2.
KOiK01869.
OMAiYEGVMLV.
OrthoDBiEOG7VB2DJ.
PhylomeDBiQ8BMJ2.
TreeFamiTF105718.

Miscellaneous databases

ChiTaRSiLars. mouse.
NextBioi358546.
PROiQ8BMJ2.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BMJ2.
CleanExiMM_LARS.
ExpressionAtlasiQ8BMJ2. baseline and differential.
GenevestigatoriQ8BMJ2.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
  2. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-972 AND LYS-1049, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiSYLC_MOUSE
AccessioniPrimary (citable) accession number: Q8BMJ2
Secondary accession number(s): Q8BKW9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: February 4, 2015
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.