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Q8BMJ2

- SYLC_MOUSE

UniProt

Q8BMJ2 - SYLC_MOUSE

Protein

Leucine--tRNA ligase, cytoplasmic

Gene

Lars

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 2 (07 Jun 2004)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei721 – 7211ATPBy similarity

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. leucine-tRNA ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. leucyl-tRNA aminoacylation Source: InterPro

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leucine--tRNA ligase, cytoplasmic (EC:6.1.1.4)
    Alternative name(s):
    Leucyl-tRNA synthetase
    Short name:
    LeuRS
    Gene namesi
    Name:Lars
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 18

    Organism-specific databases

    MGIiMGI:1913808. Lars.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11781178Leucine--tRNA ligase, cytoplasmicPRO_0000152151Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei972 – 9721N6-acetyllysine1 Publication
    Modified residuei1049 – 10491N6-acetyllysine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ8BMJ2.
    PaxDbiQ8BMJ2.
    PRIDEiQ8BMJ2.

    PTM databases

    PhosphoSiteiQ8BMJ2.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8BMJ2.
    BgeeiQ8BMJ2.
    CleanExiMM_LARS.
    GenevestigatoriQ8BMJ2.

    Interactioni

    Protein-protein interaction databases

    BioGridi223191. 2 interactions.
    IntActiQ8BMJ2. 1 interaction.
    MINTiMINT-1848936.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BMJ2.
    SMRiQ8BMJ2. Positions 13-930.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi55 – 6511"HIGH" regionAdd
    BLAST
    Motifi718 – 7225"KMSKS" region

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0495.
    GeneTreeiENSGT00390000012163.
    HOVERGENiHBG055325.
    InParanoidiQ8BMJ2.
    KOiK01869.
    OMAiYEGVMLV.
    OrthoDBiEOG7VB2DJ.
    PhylomeDBiQ8BMJ2.
    TreeFamiTF105718.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR004493. Leu-tRNA-synth_Ia_arc/euk.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 2 hits.
    [Graphical view]
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 2 hits.
    TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8BMJ2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGRKGTAKV DFLKEIEKEA QQKWEAEKVF EVSASRLEKQ KQSSKGKYFV     50
    TFPYPYMNGR LHLGHTFSLS KCEFAVGYQR LKGKSCLFPF GLHCTGMPIK 100
    ACADKLKREI ELYGCPPDFP EEEEEEEESS AKPGDIVVRD KAKGKKSKAA 150
    AKAGSSKYQW DIMKSLGLSD DDIVKFSEAE HWLDYFPPLA VQDLKTIGLK 200
    VDWRRSFITT DVNPYYDSFV RWQFLTLRER NKIKFGKRYT IYSPKDGQPC 250
    MDHDRQTGEG VGPQEYTLVK LKVLEPYPSK LSGLKGKNIF LVAATLRPET 300
    MFGQTNCWVR PDMKYIGFET ANGDIFICTQ RAARNMSYQG FTKHNGVVPV 350
    VKELMGEEIL GASLSAPLTC YKVVYVLPML TIKEDKGTGV VTSVPSDSPD 400
    DLAALRDLKK KQALRTKFGI RDDMVLPFEP VPVLEIPGIG NLPAVTVCDE 450
    LKIQSQNDRE KLAEAKEKLY LRGFYDGVML VDGFKGQKIQ HVKKTIQKNM 500
    IDAGDALIYM EPEKQVMSRS ADECVVALCD QWYLDYGDEN WKKQTFQCLK 550
    NMETFCEESR KNFEASLDWL QEHACSRTYG LGTRLPWDEQ WLIESLSDST 600
    IYMAFYTVAH LLQGGDLNGQ AESPLGIRPQ QMTKDVWDYV FFKDAPFPKT 650
    QIPKEKLDQL KQEFEFWYPV DLRASGKDLI PNHLSYYIYN HVAMWPEQSD 700
    KWPVSVRANG HLLLNSEKMS KSTGNFLTLS QAVDKFSADG MRLALADAGD 750
    TVEDANFVEA MADAGILRLY TWVEWVKEML ASCSSLRSGP ADSFNDRVFA 800
    SEMNAGIIKT DQNYEKMMFK EALKTGFFEF QAAKDKYREL ATEGMHRELV 850
    FRFIEVQTIL LTPFCPHLCE HIWTLLGKPD SIMHASWPVA GPVDESLIRS 900
    SQYLMEVAHD LRLRLKNYMM PAKGKKTDKQ PAQRPSHCTI YVAKNYPVWQ 950
    HITLTTLRSH FEANNGKLPD NKVIASELGS LPELKKYMKK VMPFVAMIKE 1000
    NMEKKGPRVL DLELEFDEQA VLMENIVYLT NSLELEHIEV KFASEAEDKV 1050
    REECCPGKPL NVFRTEPGVP VSLVNPQPSS GHFSTKIDIR QGDSCESIIR 1100
    RLMKTDRGIK DLSKVKLMRF DDPLLGPRRV PVLGREHSEK TLISENAVFH 1150
    VDLVSKKVHL TENGLRTDIG DTMVYLVH 1178
    Length:1,178
    Mass (Da):134,192
    Last modified:June 7, 2004 - v2
    Checksum:i1186583D5237FFC1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti218 – 2181S → P in BAC33766. (PubMed:16141072)Curated
    Sequence conflicti869 – 8691C → S in BAC33766. (PubMed:16141072)Curated
    Sequence conflicti954 – 9541L → Q in BAC27133. (PubMed:16141072)Curated
    Sequence conflicti1127 – 11271P → H in BAC27133. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK030778 mRNA. Translation: BAC27133.1.
    AK049472 mRNA. Translation: BAC33766.1.
    CCDSiCCDS37796.1.
    RefSeqiNP_598898.2. NM_134137.2.
    UniGeneiMm.312170.

    Genome annotation databases

    EnsembliENSMUST00000097590; ENSMUSP00000095197; ENSMUSG00000024493.
    GeneIDi107045.
    KEGGimmu:107045.
    UCSCiuc008etp.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK030778 mRNA. Translation: BAC27133.1 .
    AK049472 mRNA. Translation: BAC33766.1 .
    CCDSi CCDS37796.1.
    RefSeqi NP_598898.2. NM_134137.2.
    UniGenei Mm.312170.

    3D structure databases

    ProteinModelPortali Q8BMJ2.
    SMRi Q8BMJ2. Positions 13-930.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 223191. 2 interactions.
    IntActi Q8BMJ2. 1 interaction.
    MINTi MINT-1848936.

    PTM databases

    PhosphoSitei Q8BMJ2.

    Proteomic databases

    MaxQBi Q8BMJ2.
    PaxDbi Q8BMJ2.
    PRIDEi Q8BMJ2.

    Protocols and materials databases

    DNASUi 107045.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000097590 ; ENSMUSP00000095197 ; ENSMUSG00000024493 .
    GeneIDi 107045.
    KEGGi mmu:107045.
    UCSCi uc008etp.1. mouse.

    Organism-specific databases

    CTDi 51520.
    MGIi MGI:1913808. Lars.

    Phylogenomic databases

    eggNOGi COG0495.
    GeneTreei ENSGT00390000012163.
    HOVERGENi HBG055325.
    InParanoidi Q8BMJ2.
    KOi K01869.
    OMAi YEGVMLV.
    OrthoDBi EOG7VB2DJ.
    PhylomeDBi Q8BMJ2.
    TreeFami TF105718.

    Miscellaneous databases

    ChiTaRSi LARS. mouse.
    NextBioi 358546.
    PROi Q8BMJ2.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8BMJ2.
    Bgeei Q8BMJ2.
    CleanExi MM_LARS.
    Genevestigatori Q8BMJ2.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR004493. Leu-tRNA-synth_Ia_arc/euk.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 2 hits.
    [Graphical view ]
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 2 hits.
    TIGRFAMsi TIGR00395. leuS_arch. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
    2. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-972 AND LYS-1049, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiSYLC_MOUSE
    AccessioniPrimary (citable) accession number: Q8BMJ2
    Secondary accession number(s): Q8BKW9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 7, 2004
    Last sequence update: June 7, 2004
    Last modified: October 1, 2014
    This is version 96 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3