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Protein

Flap endonuclease GEN homolog 1

Gene

Gen1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Endonuclease which resolves Holliday junctions by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombination and DNA repair, and their resolution is necessary for proper chromosome segregation (By similarity).By similarity

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi30Magnesium 1By similarity1
Metal bindingi75Magnesium 1By similarity1
Metal bindingi134Magnesium 1By similarity1
Metal bindingi136Magnesium 1By similarity1
Metal bindingi155Magnesium 2By similarity1
Metal bindingi157Magnesium 2By similarity1
Metal bindingi208Magnesium 2By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processDNA damage, DNA repair
LigandMagnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-5693568 Resolution of D-loop Structures through Holliday Junction Intermediates

Names & Taxonomyi

Protein namesi
Recommended name:
Flap endonuclease GEN homolog 1 (EC:3.1.-.-)
Gene namesi
Name:Gen1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:2443149 Gen1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003141471 – 908Flap endonuclease GEN homolog 1Add BLAST908

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei794PhosphoserineCombined sources1
Modified residuei795PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8BMI4
PeptideAtlasiQ8BMI4
PRIDEiQ8BMI4

PTM databases

iPTMnetiQ8BMI4
PhosphoSitePlusiQ8BMI4

Expressioni

Tissue specificityi

Expressed in bone marrow and testis and to a lesser extent in thymus, spleen, brain and colon.1 Publication

Gene expression databases

BgeeiENSMUSG00000051235
CleanExiMM_GEN1
ExpressionAtlasiQ8BMI4 baseline and differential
GenevisibleiQ8BMI4 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000132098

Structurei

3D structure databases

ProteinModelPortaliQ8BMI4
SMRiQ8BMI4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 96N-domainAdd BLAST96
Regioni122 – 212I-domainAdd BLAST91

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2519 Eukaryota
COG0258 LUCA
GeneTreeiENSGT00640000091478
HOGENOMiHOG000060137
HOVERGENiHBG096402
InParanoidiQ8BMI4
KOiK15338
OMAiEKMEWPN
OrthoDBiEOG091G07ZH
PhylomeDBiQ8BMI4
TreeFamiTF323403

Family and domain databases

InterProiView protein in InterPro
IPR036279 5-3_exonuclease_C_sf
IPR008918 HhH2
IPR029060 PIN-like_dom_sf
IPR006086 XPG-I_dom
IPR006084 XPG/Rad2
IPR006085 XPG_DNA_repair_N
PANTHERiPTHR11081 PTHR11081, 1 hit
PfamiView protein in Pfam
PF00867 XPG_I, 1 hit
PF00752 XPG_N, 1 hit
PRINTSiPR00853 XPGRADSUPER
SMARTiView protein in SMART
SM00279 HhH2, 1 hit
SM00484 XPGI, 1 hit
SM00485 XPGN, 1 hit
SUPFAMiSSF47807 SSF47807, 2 hits
SSF88723 SSF88723, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BMI4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGVNDLWQIL EPVKQHIHLQ DLSGKTIAVD LSLWVCEAQT VKKMIGTVKK
60 70 80 90 100
PHLRNLFFRI SYLTQMNVKL VFVMEGEPPM LKADVISKRT QTRYGPSGKS
110 120 130 140 150
RSQKTGRSHF KSVLRECLEM LECLGMPWVQ AAGEAEAMCA YLNASGHVDG
160 170 180 190 200
CLTNDGDAFL YGAQTVYRNF TMNTKDPHVD CYTISSIKSK LGLDRDALVG
210 220 230 240 250
LAVLLGCDYL PKGVPGVGKE QALKLLQIFK GQSLLQRFNQ WIEDPCYSVP
260 270 280 290 300
QSAPKKVVHC SVCSHPGSPK DHERNGCILC KSDKYCEPHD YDYLCPCEWH
310 320 330 340 350
QTDHNRHLSE IENNIKKKAC SCEGFPFHEV IQEFLLNKNK MLKPITYQRP
360 370 380 390 400
DLLLFQRFTV QKMEWPSHYA CEKLLVLLTR YDMIERKHGR KTSNQLQPIR
410 420 430 440 450
IVKPRVRNGV HCLEIEWEKP EHYVVEDGDP GKLSLLTMEE ASLFEAAYPD
460 470 480 490 500
AVAVYQKQLS ETKGRKQKSM KNKPKGSHLP EADDVINSQS LMTLKPTSKA
510 520 530 540 550
FPKQNPKINL ENSPDPILAQ ESTSPSLNSF VSPENAPCLN LQEQLVPSPR
560 570 580 590 600
TLAIKQSKDV SHFLVSECSQ PSSSSHDISV ITDLQLSTID WAGTSFSNSP
610 620 630 640 650
AVQRNTFSQD LASESESSAI LPDFEQLSYE SEQGTSDSEG SGRDLQQSNP
660 670 680 690 700
EEQLLSGISA LHLHDLPLKE RIRIKSSCPQ YNVGADAGLE SLPLKLKGSC
710 720 730 740 750
IAYSSSDGSS NFSKDLTGVY LHKESRNSKV LDSRLQENCG ANTSLPYSFS
760 770 780 790 800
DKAVKTSSLQ VGLPTAAIPH NPRVAVKTTK NLVMKNSVCL ERDSSDEDNA
810 820 830 840 850
PGSWKSKYTA PEMKHSSQKH SLVHVRDSTH NKLRNPKVES KETKLCNESF
860 870 880 890 900
KTAEDEENGF SDLGRSPQSF RPCHDKDENS TASWENPLPL RQRLKLRFQN

TQSGFYNT
Length:908
Mass (Da):101,779
Last modified:January 15, 2008 - v2
Checksum:i1D3D1A1A10601AD3
GO
Isoform 2 (identifier: Q8BMI4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-65: Missing.

Note: No experimental confirmation available.
Show »
Length:843
Mass (Da):94,266
Checksum:iE6C351ECE2A2FBD0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti48V → I in BAC27242 (PubMed:16141072).Curated1
Sequence conflicti716L → H in BAC27207 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0302161 – 65Missing in isoform 2. 1 PublicationAdd BLAST65

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030995 mRNA Translation: BAC27207.1
AK031077 mRNA Translation: BAC27242.1
AK158255 mRNA Translation: BAE34428.1
BC090653 mRNA Translation: AAH90653.1
BC138157 mRNA Translation: AAI38158.1
BC138158 mRNA Translation: AAI38159.1
CCDSiCCDS36403.1 [Q8BMI4-1]
RefSeqiNP_796305.3, NM_177331.4 [Q8BMI4-1]
XP_006515102.1, XM_006515039.3 [Q8BMI4-1]
XP_006515103.1, XM_006515040.3 [Q8BMI4-1]
XP_006515104.1, XM_006515041.2 [Q8BMI4-1]
XP_006515105.1, XM_006515042.3 [Q8BMI4-2]
UniGeneiMm.215295

Genome annotation databases

EnsembliENSMUST00000166117; ENSMUSP00000132098; ENSMUSG00000051235 [Q8BMI4-1]
GeneIDi209334
KEGGimmu:209334
UCSCiuc007nav.1 mouse [Q8BMI4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGEN_MOUSE
AccessioniPrimary (citable) accession number: Q8BMI4
Secondary accession number(s): B2RR04
, Q3TYY2, Q5CZW6, Q8C0J0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: March 28, 2018
This is version 111 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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