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Protein

Flap endonuclease GEN homolog 1

Gene

Gen1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Endonuclease which resolves Holliday junctions by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombination and DNA repair, and their resolution is necessary for proper chromosome segregation (By similarity).By similarity

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi30 – 301Magnesium 1By similarity
Metal bindingi75 – 751Magnesium 1By similarity
Metal bindingi134 – 1341Magnesium 1By similarity
Metal bindingi136 – 1361Magnesium 1By similarity
Metal bindingi155 – 1551Magnesium 2By similarity
Metal bindingi157 – 1571Magnesium 2By similarity
Metal bindingi208 – 2081Magnesium 2By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-5693568. Resolution of D-loop Structures through Holliday Junction Intermediates.

Names & Taxonomyi

Protein namesi
Recommended name:
Flap endonuclease GEN homolog 1 (EC:3.1.-.-)
Gene namesi
Name:Gen1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:2443149. Gen1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 908908Flap endonuclease GEN homolog 1PRO_0000314147Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei794 – 7941PhosphoserineCombined sources
Modified residuei795 – 7951PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8BMI4.
MaxQBiQ8BMI4.
PaxDbiQ8BMI4.
PRIDEiQ8BMI4.

PTM databases

iPTMnetiQ8BMI4.
PhosphoSiteiQ8BMI4.

Expressioni

Gene expression databases

BgeeiQ8BMI4.
CleanExiMM_GEN1.
GenevisibleiQ8BMI4. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000132098.

Structurei

3D structure databases

ProteinModelPortaliQ8BMI4.
SMRiQ8BMI4. Positions 1-259.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 9696N-domainAdd
BLAST
Regioni122 – 21291I-domainAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2519. Eukaryota.
COG0258. LUCA.
GeneTreeiENSGT00640000091478.
HOGENOMiHOG000060137.
HOVERGENiHBG096402.
InParanoidiQ8BMI4.
KOiK15338.
OMAiFFRISYL.
OrthoDBiEOG7C5M86.
PhylomeDBiQ8BMI4.
TreeFamiTF323403.

Family and domain databases

Gene3Di3.40.50.1010. 1 hit.
InterProiIPR020045. 5-3_exonuclease_C.
IPR008918. HhH2.
IPR029060. PIN_domain-like.
IPR006086. XPG-I_dom.
IPR006084. XPG/Rad2.
IPR006085. XPG_DNA_repair_N.
[Graphical view]
PfamiPF00867. XPG_I. 1 hit.
PF00752. XPG_N. 1 hit.
[Graphical view]
PRINTSiPR00853. XPGRADSUPER.
SMARTiSM00279. HhH2. 1 hit.
SM00484. XPGI. 1 hit.
SM00485. XPGN. 1 hit.
[Graphical view]
SUPFAMiSSF47807. SSF47807. 2 hits.
SSF88723. SSF88723. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BMI4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGVNDLWQIL EPVKQHIHLQ DLSGKTIAVD LSLWVCEAQT VKKMIGTVKK
60 70 80 90 100
PHLRNLFFRI SYLTQMNVKL VFVMEGEPPM LKADVISKRT QTRYGPSGKS
110 120 130 140 150
RSQKTGRSHF KSVLRECLEM LECLGMPWVQ AAGEAEAMCA YLNASGHVDG
160 170 180 190 200
CLTNDGDAFL YGAQTVYRNF TMNTKDPHVD CYTISSIKSK LGLDRDALVG
210 220 230 240 250
LAVLLGCDYL PKGVPGVGKE QALKLLQIFK GQSLLQRFNQ WIEDPCYSVP
260 270 280 290 300
QSAPKKVVHC SVCSHPGSPK DHERNGCILC KSDKYCEPHD YDYLCPCEWH
310 320 330 340 350
QTDHNRHLSE IENNIKKKAC SCEGFPFHEV IQEFLLNKNK MLKPITYQRP
360 370 380 390 400
DLLLFQRFTV QKMEWPSHYA CEKLLVLLTR YDMIERKHGR KTSNQLQPIR
410 420 430 440 450
IVKPRVRNGV HCLEIEWEKP EHYVVEDGDP GKLSLLTMEE ASLFEAAYPD
460 470 480 490 500
AVAVYQKQLS ETKGRKQKSM KNKPKGSHLP EADDVINSQS LMTLKPTSKA
510 520 530 540 550
FPKQNPKINL ENSPDPILAQ ESTSPSLNSF VSPENAPCLN LQEQLVPSPR
560 570 580 590 600
TLAIKQSKDV SHFLVSECSQ PSSSSHDISV ITDLQLSTID WAGTSFSNSP
610 620 630 640 650
AVQRNTFSQD LASESESSAI LPDFEQLSYE SEQGTSDSEG SGRDLQQSNP
660 670 680 690 700
EEQLLSGISA LHLHDLPLKE RIRIKSSCPQ YNVGADAGLE SLPLKLKGSC
710 720 730 740 750
IAYSSSDGSS NFSKDLTGVY LHKESRNSKV LDSRLQENCG ANTSLPYSFS
760 770 780 790 800
DKAVKTSSLQ VGLPTAAIPH NPRVAVKTTK NLVMKNSVCL ERDSSDEDNA
810 820 830 840 850
PGSWKSKYTA PEMKHSSQKH SLVHVRDSTH NKLRNPKVES KETKLCNESF
860 870 880 890 900
KTAEDEENGF SDLGRSPQSF RPCHDKDENS TASWENPLPL RQRLKLRFQN

TQSGFYNT
Length:908
Mass (Da):101,779
Last modified:January 15, 2008 - v2
Checksum:i1D3D1A1A10601AD3
GO
Isoform 2 (identifier: Q8BMI4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-65: Missing.

Note: No experimental confirmation available.
Show »
Length:843
Mass (Da):94,266
Checksum:iE6C351ECE2A2FBD0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti48 – 481V → I in BAC27242 (PubMed:16141072).Curated
Sequence conflicti716 – 7161L → H in BAC27207 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 6565Missing in isoform 2. 1 PublicationVSP_030216Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030995 mRNA. Translation: BAC27207.1.
AK031077 mRNA. Translation: BAC27242.1.
AK158255 mRNA. Translation: BAE34428.1.
BC090653 mRNA. Translation: AAH90653.1.
BC138157 mRNA. Translation: AAI38158.1.
BC138158 mRNA. Translation: AAI38159.1.
CCDSiCCDS36403.1. [Q8BMI4-1]
RefSeqiNP_796305.3. NM_177331.4. [Q8BMI4-1]
XP_006515102.1. XM_006515039.2. [Q8BMI4-1]
XP_006515103.1. XM_006515040.2. [Q8BMI4-1]
XP_006515104.1. XM_006515041.2. [Q8BMI4-1]
XP_006515105.1. XM_006515042.2. [Q8BMI4-2]
UniGeneiMm.215295.

Genome annotation databases

EnsembliENSMUST00000166117; ENSMUSP00000132098; ENSMUSG00000051235. [Q8BMI4-1]
GeneIDi209334.
KEGGimmu:209334.
UCSCiuc007nav.1. mouse. [Q8BMI4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030995 mRNA. Translation: BAC27207.1.
AK031077 mRNA. Translation: BAC27242.1.
AK158255 mRNA. Translation: BAE34428.1.
BC090653 mRNA. Translation: AAH90653.1.
BC138157 mRNA. Translation: AAI38158.1.
BC138158 mRNA. Translation: AAI38159.1.
CCDSiCCDS36403.1. [Q8BMI4-1]
RefSeqiNP_796305.3. NM_177331.4. [Q8BMI4-1]
XP_006515102.1. XM_006515039.2. [Q8BMI4-1]
XP_006515103.1. XM_006515040.2. [Q8BMI4-1]
XP_006515104.1. XM_006515041.2. [Q8BMI4-1]
XP_006515105.1. XM_006515042.2. [Q8BMI4-2]
UniGeneiMm.215295.

3D structure databases

ProteinModelPortaliQ8BMI4.
SMRiQ8BMI4. Positions 1-259.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000132098.

PTM databases

iPTMnetiQ8BMI4.
PhosphoSiteiQ8BMI4.

Proteomic databases

EPDiQ8BMI4.
MaxQBiQ8BMI4.
PaxDbiQ8BMI4.
PRIDEiQ8BMI4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000166117; ENSMUSP00000132098; ENSMUSG00000051235. [Q8BMI4-1]
GeneIDi209334.
KEGGimmu:209334.
UCSCiuc007nav.1. mouse. [Q8BMI4-1]

Organism-specific databases

CTDi348654.
MGIiMGI:2443149. Gen1.

Phylogenomic databases

eggNOGiKOG2519. Eukaryota.
COG0258. LUCA.
GeneTreeiENSGT00640000091478.
HOGENOMiHOG000060137.
HOVERGENiHBG096402.
InParanoidiQ8BMI4.
KOiK15338.
OMAiFFRISYL.
OrthoDBiEOG7C5M86.
PhylomeDBiQ8BMI4.
TreeFamiTF323403.

Enzyme and pathway databases

ReactomeiR-MMU-5693568. Resolution of D-loop Structures through Holliday Junction Intermediates.

Miscellaneous databases

ChiTaRSiGen1. mouse.
PROiQ8BMI4.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BMI4.
CleanExiMM_GEN1.
GenevisibleiQ8BMI4. MM.

Family and domain databases

Gene3Di3.40.50.1010. 1 hit.
InterProiIPR020045. 5-3_exonuclease_C.
IPR008918. HhH2.
IPR029060. PIN_domain-like.
IPR006086. XPG-I_dom.
IPR006084. XPG/Rad2.
IPR006085. XPG_DNA_repair_N.
[Graphical view]
PfamiPF00867. XPG_I. 1 hit.
PF00752. XPG_N. 1 hit.
[Graphical view]
PRINTSiPR00853. XPGRADSUPER.
SMARTiSM00279. HhH2. 1 hit.
SM00484. XPGI. 1 hit.
SM00485. XPGN. 1 hit.
[Graphical view]
SUPFAMiSSF47807. SSF47807. 2 hits.
SSF88723. SSF88723. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-804 (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Forelimb, Inner ear and Thymus.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Brain and Head.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-794 AND SER-795, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Spleen and Testis.

Entry informationi

Entry nameiGEN_MOUSE
AccessioniPrimary (citable) accession number: Q8BMI4
Secondary accession number(s): B2RR04
, Q3TYY2, Q5CZW6, Q8C0J0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: June 8, 2016
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.