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Protein

ADP-ribosylation factor-binding protein GGA3

Gene

Gga3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • intracellular protein transport Source: InterPro
  • positive regulation of protein catabolic process Source: MGI
  • vesicle-mediated transport Source: InterPro

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-8875656 MET receptor recycling

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor-binding protein GGA3
Alternative name(s):
Golgi-localized, gamma ear-containing, ARF-binding protein 3
Gene namesi
Name:Gga3
Synonyms:Kiaa0154
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2384159 Gga3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002126851 – 718ADP-ribosylation factor-binding protein GGA3Add BLAST718

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei159PhosphoserineBy similarity1
Modified residuei275PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by CK2 and dephosphorylated by PP2A. Phosphorylation of GGA3 allows the internal AC-LL motif to bind the VHS domain and to inhibit the recognition of cargo signals (By similarity).By similarity
Ubiquitinated.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8BMI3
MaxQBiQ8BMI3
PaxDbiQ8BMI3
PRIDEiQ8BMI3

PTM databases

iPTMnetiQ8BMI3
PhosphoSitePlusiQ8BMI3

Expressioni

Gene expression databases

BgeeiENSMUSG00000020740
ExpressionAtlasiQ8BMI3 baseline and differential
GenevisibleiQ8BMI3 MM

Interactioni

Subunit structurei

Monomer. Interacts with GGA1 and GGA2. Binds to clathrin and activated ARFs. Binds RABEP1 and RABGEF1. Interacts with the type-I membrane proteins SORT1, SORL1, LRP3, M6PR/CD-MPR, IGF2R/CI-MPR and BACE1. Binds the accessory proteins P56, P200, SYNRG, EPN4, NECAP1, NECAP2 and AFTPH/aftiphilin. Interacts with TSG101 and UBC (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi234429, 3 interactors
ELMiQ8BMI3
IntActiQ8BMI3, 1 interactor
MINTiQ8BMI3
STRINGi10090.ENSMUSP00000019135

Structurei

3D structure databases

ProteinModelPortaliQ8BMI3
SMRiQ8BMI3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 146VHSPROSITE-ProRule annotationAdd BLAST131
Domaini171 – 298GATPROSITE-ProRule annotationAdd BLAST128
Domaini589 – 710GAEPROSITE-ProRule annotationAdd BLAST122

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni299 – 588Unstructured hingeAdd BLAST290

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi387 – 391AutoinhibitoryBy similarity5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi335 – 500Pro-richAdd BLAST166

Domaini

The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).By similarity
The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis (By similarity).By similarity
The unstructured hinge region contains clathrin-binding and an autoinhibitory (AC-LL) motifs.By similarity
The GAE domain binds accessory proteins regulating GGAs function.By similarity

Sequence similaritiesi

Belongs to the GGA protein family.Curated

Phylogenomic databases

eggNOGiKOG1087 Eukaryota
ENOG410Y26G LUCA
GeneTreeiENSGT00870000136446
HOGENOMiHOG000231169
HOVERGENiHBG015945
InParanoidiQ8BMI3
KOiK12404
OMAiHYSKEDS
OrthoDBiEOG091G0BQK
TreeFamiTF318574

Family and domain databases

Gene3Di1.20.58.160, 1 hit
1.25.40.90, 1 hit
InterProiView protein in InterPro
IPR008152 Clathrin_a/b/g-adaptin_app_Ig
IPR013041 Clathrin_app_Ig-like_sf
IPR008942 ENTH_VHS
IPR008153 GAE_dom
IPR004152 GAT_dom
IPR038425 GAT_sf
IPR027422 GGA3
IPR002014 VHS_dom
PANTHERiPTHR13856:SF34 PTHR13856:SF34, 1 hit
PfamiView protein in Pfam
PF02883 Alpha_adaptinC2, 1 hit
PF03127 GAT, 1 hit
PF00790 VHS, 1 hit
SMARTiView protein in SMART
SM00809 Alpha_adaptinC2, 1 hit
SM00288 VHS, 1 hit
SUPFAMiSSF48464 SSF48464, 1 hit
SSF49348 SSF49348, 1 hit
PROSITEiView protein in PROSITE
PS50180 GAE, 1 hit
PS50909 GAT, 1 hit
PS50179 VHS, 1 hit

Sequencei

Sequence statusi: Complete.

Q8BMI3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEAEGESLE SWLNKATNPS NRQEDWEYII GFCDQINKEL EGPQIAVRLL
60 70 80 90 100
AHKIQSPQEW EAVQALTVLE ACMKNCGRRL HNEVGKFRFL NELIKVVSPK
110 120 130 140 150
YLGDRVSEKV KTKVIELLFS WTLALPEEAK IKDAYHMLKR QGIVQSDPPI
160 170 180 190 200
PMDRTLIPSP PPRPKNPVFD DEEKSKLLAR LLKSKNPDDL QEANRLIKSM
210 220 230 240 250
VKEDEARIQK VTKRLHTLEE VNNNVKLLHE MLLHYSQEYS SDADKELMKE
260 270 280 290 300
LFDRCENKRR TLFKLASETE DNDNSLGDIL QASDNLSRVI NSYKTIIEGQ
310 320 330 340 350
IVNGEVTTST MPDSEGNSHC GNQGALIDLA ELDAPSNSSP ALAPPTSGIP
360 370 380 390 400
ILPPPPQTSG PPRSRSSSQA EAPPGSDSTN NALSLLDEEL LCLGLTDPAP
410 420 430 440 450
TAPKESPGSS QWHLFQNEPP SDLDFFSPRP VPAASCPSDG PQLPPPVSTS
460 470 480 490 500
SMSQAPLPAA FPAPVVPASA PTHSTGSFMF SSGPAPALAP KAEPKGPEYP
510 520 530 540 550
SSSTSHRLDA LDQLLEEAKV TSGLVKPVSC FSPGPTASPL LPASAPARPL
560 570 580 590 600
LPFSTGPGSP LFQSQGSPQK GPELSLASVH VPLESIKPSS ALPVTAYDKN
610 620 630 640 650
GFRILFHFAK ECPPGRPDVL VVVVSMLNTA PLPVKSIVLQ AAVPKSMKVK
660 670 680 690 700
LQPPSGTELS PFSPIQPPAA ITQVMLLANP MKEKVRLRYK LTFALGEQLS
710
TELGEVDQFP PVEQWGNL
Length:718
Mass (Da):77,973
Last modified:July 27, 2011 - v2
Checksum:i265A4993609D89B9
GO

Sequence cautioni

The sequence BAC65494 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti151P → T in BAC27246 (PubMed:14621295).Curated1
Sequence conflicti433A → V in BAC65494 (PubMed:14621295).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122212 mRNA Translation: BAC65494.2 Different initiation.
AK031086 mRNA Translation: BAC27246.1
AK132183 mRNA Translation: BAE21019.1
AL645470 Genomic DNA No translation available.
CCDSiCCDS25641.1
RefSeqiNP_766636.2, NM_173048.3
UniGeneiMm.119479

Genome annotation databases

EnsembliENSMUST00000019135; ENSMUSP00000019135; ENSMUSG00000020740
GeneIDi260302
KEGGimmu:260302
UCSCiuc007mhy.2 mouse

Similar proteinsi

Entry informationi

Entry nameiGGA3_MOUSE
AccessioniPrimary (citable) accession number: Q8BMI3
Secondary accession number(s): Q3V1Y0, Q80U71
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: July 27, 2011
Last modified: March 28, 2018
This is version 124 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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