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Protein

ADP-ribosylation factor-binding protein GGA3

Gene

Gga3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • intracellular protein transport Source: InterPro
  • positive regulation of protein catabolic process Source: MGI
  • vesicle-mediated transport Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor-binding protein GGA3
Alternative name(s):
Golgi-localized, gamma ear-containing, ARF-binding protein 3
Gene namesi
Name:Gga3
Synonyms:Kiaa0154
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2384159. Gga3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 718718ADP-ribosylation factor-binding protein GGA3PRO_0000212685Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei159 – 1591PhosphoserineBy similarity
Modified residuei275 – 2751PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated by CK2 and dephosphorylated by PP2A. Phosphorylation of GGA3 allows the internal AC-LL motif to bind the VHS domain and to inhibit the recognition of cargo signals (By similarity).By similarity
Ubiquitinated.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8BMI3.
MaxQBiQ8BMI3.
PaxDbiQ8BMI3.
PRIDEiQ8BMI3.

PTM databases

iPTMnetiQ8BMI3.
PhosphoSiteiQ8BMI3.

Expressioni

Gene expression databases

BgeeiENSMUSG00000020740.
ExpressionAtlasiQ8BMI3. baseline and differential.
GenevisibleiQ8BMI3. MM.

Interactioni

Subunit structurei

Monomer. Interacts with GGA1 and GGA2. Binds to clathrin and activated ARFs. Binds RABEP1 and RABGEF1. Interacts with the type-I membrane proteins SORT1, SORL1, LRP3, M6PR/CD-MPR, IGF2R/CI-MPR and BACE1. Binds the accessory proteins P56, P200, SYNRG, EPN4, NECAP1, NECAP2 and AFTPH/aftiphilin. Interacts with TSG101 and UBC (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ8BMI3. 1 interaction.
MINTiMINT-4111541.
STRINGi10090.ENSMUSP00000019135.

Structurei

3D structure databases

ProteinModelPortaliQ8BMI3.
SMRiQ8BMI3. Positions 1-157, 168-300, 574-718.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini16 – 146131VHSPROSITE-ProRule annotationAdd
BLAST
Domaini171 – 298128GATPROSITE-ProRule annotationAdd
BLAST
Domaini589 – 710122GAEPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni299 – 588290Unstructured hingeAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi387 – 3915AutoinhibitoryBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi335 – 500166Pro-richAdd
BLAST

Domaini

The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).By similarity
The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis (By similarity).By similarity
The unstructured hinge region contains clathrin-binding and an autoinhibitory (AC-LL) motifs.By similarity
The GAE domain binds accessory proteins regulating GGAs function.By similarity

Sequence similaritiesi

Belongs to the GGA protein family.Curated
Contains 1 GAE domain.PROSITE-ProRule annotation
Contains 1 GAT domain.PROSITE-ProRule annotation
Contains 1 VHS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1087. Eukaryota.
ENOG410Y26G. LUCA.
GeneTreeiENSGT00700000104396.
HOGENOMiHOG000231169.
HOVERGENiHBG015945.
InParanoidiQ8BMI3.
KOiK12404.
OMAiAPWAQTR.
OrthoDBiEOG091G0BQK.
TreeFamiTF318574.

Family and domain databases

Gene3Di1.20.58.160. 1 hit.
1.25.40.90. 1 hit.
2.60.40.1230. 1 hit.
InterProiIPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR008153. Clathrin_g-adaptin_app.
IPR013041. Coatomer/clathrin_app_Ig-like.
IPR008942. ENTH_VHS.
IPR004152. GAT_dom.
IPR027422. GGA3.
IPR002014. VHS_dom.
[Graphical view]
PANTHERiPTHR13856:SF34. PTHR13856:SF34. 3 hits.
PfamiPF02883. Alpha_adaptinC2. 1 hit.
PF03127. GAT. 1 hit.
PF00790. VHS. 1 hit.
[Graphical view]
SMARTiSM00809. Alpha_adaptinC2. 1 hit.
SM00288. VHS. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
SSF49348. SSF49348. 1 hit.
PROSITEiPS50180. GAE. 1 hit.
PS50909. GAT. 1 hit.
PS50179. VHS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BMI3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEAEGESLE SWLNKATNPS NRQEDWEYII GFCDQINKEL EGPQIAVRLL
60 70 80 90 100
AHKIQSPQEW EAVQALTVLE ACMKNCGRRL HNEVGKFRFL NELIKVVSPK
110 120 130 140 150
YLGDRVSEKV KTKVIELLFS WTLALPEEAK IKDAYHMLKR QGIVQSDPPI
160 170 180 190 200
PMDRTLIPSP PPRPKNPVFD DEEKSKLLAR LLKSKNPDDL QEANRLIKSM
210 220 230 240 250
VKEDEARIQK VTKRLHTLEE VNNNVKLLHE MLLHYSQEYS SDADKELMKE
260 270 280 290 300
LFDRCENKRR TLFKLASETE DNDNSLGDIL QASDNLSRVI NSYKTIIEGQ
310 320 330 340 350
IVNGEVTTST MPDSEGNSHC GNQGALIDLA ELDAPSNSSP ALAPPTSGIP
360 370 380 390 400
ILPPPPQTSG PPRSRSSSQA EAPPGSDSTN NALSLLDEEL LCLGLTDPAP
410 420 430 440 450
TAPKESPGSS QWHLFQNEPP SDLDFFSPRP VPAASCPSDG PQLPPPVSTS
460 470 480 490 500
SMSQAPLPAA FPAPVVPASA PTHSTGSFMF SSGPAPALAP KAEPKGPEYP
510 520 530 540 550
SSSTSHRLDA LDQLLEEAKV TSGLVKPVSC FSPGPTASPL LPASAPARPL
560 570 580 590 600
LPFSTGPGSP LFQSQGSPQK GPELSLASVH VPLESIKPSS ALPVTAYDKN
610 620 630 640 650
GFRILFHFAK ECPPGRPDVL VVVVSMLNTA PLPVKSIVLQ AAVPKSMKVK
660 670 680 690 700
LQPPSGTELS PFSPIQPPAA ITQVMLLANP MKEKVRLRYK LTFALGEQLS
710
TELGEVDQFP PVEQWGNL
Length:718
Mass (Da):77,973
Last modified:July 27, 2011 - v2
Checksum:i265A4993609D89B9
GO

Sequence cautioni

The sequence BAC65494 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti151 – 1511P → T in BAC27246 (PubMed:14621295).Curated
Sequence conflicti433 – 4331A → V in BAC65494 (PubMed:14621295).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122212 mRNA. Translation: BAC65494.2. Different initiation.
AK031086 mRNA. Translation: BAC27246.1.
AK132183 mRNA. Translation: BAE21019.1.
AL645470 Genomic DNA. Translation: CAM23010.1.
CCDSiCCDS25641.1.
RefSeqiNP_766636.2. NM_173048.3.
UniGeneiMm.119479.

Genome annotation databases

EnsembliENSMUST00000019135; ENSMUSP00000019135; ENSMUSG00000020740.
GeneIDi260302.
KEGGimmu:260302.
UCSCiuc007mhy.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122212 mRNA. Translation: BAC65494.2. Different initiation.
AK031086 mRNA. Translation: BAC27246.1.
AK132183 mRNA. Translation: BAE21019.1.
AL645470 Genomic DNA. Translation: CAM23010.1.
CCDSiCCDS25641.1.
RefSeqiNP_766636.2. NM_173048.3.
UniGeneiMm.119479.

3D structure databases

ProteinModelPortaliQ8BMI3.
SMRiQ8BMI3. Positions 1-157, 168-300, 574-718.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BMI3. 1 interaction.
MINTiMINT-4111541.
STRINGi10090.ENSMUSP00000019135.

PTM databases

iPTMnetiQ8BMI3.
PhosphoSiteiQ8BMI3.

Proteomic databases

EPDiQ8BMI3.
MaxQBiQ8BMI3.
PaxDbiQ8BMI3.
PRIDEiQ8BMI3.

Protocols and materials databases

DNASUi260302.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000019135; ENSMUSP00000019135; ENSMUSG00000020740.
GeneIDi260302.
KEGGimmu:260302.
UCSCiuc007mhy.2. mouse.

Organism-specific databases

CTDi23163.
MGIiMGI:2384159. Gga3.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1087. Eukaryota.
ENOG410Y26G. LUCA.
GeneTreeiENSGT00700000104396.
HOGENOMiHOG000231169.
HOVERGENiHBG015945.
InParanoidiQ8BMI3.
KOiK12404.
OMAiAPWAQTR.
OrthoDBiEOG091G0BQK.
TreeFamiTF318574.

Miscellaneous databases

PROiQ8BMI3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020740.
ExpressionAtlasiQ8BMI3. baseline and differential.
GenevisibleiQ8BMI3. MM.

Family and domain databases

Gene3Di1.20.58.160. 1 hit.
1.25.40.90. 1 hit.
2.60.40.1230. 1 hit.
InterProiIPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR008153. Clathrin_g-adaptin_app.
IPR013041. Coatomer/clathrin_app_Ig-like.
IPR008942. ENTH_VHS.
IPR004152. GAT_dom.
IPR027422. GGA3.
IPR002014. VHS_dom.
[Graphical view]
PANTHERiPTHR13856:SF34. PTHR13856:SF34. 3 hits.
PfamiPF02883. Alpha_adaptinC2. 1 hit.
PF03127. GAT. 1 hit.
PF00790. VHS. 1 hit.
[Graphical view]
SMARTiSM00809. Alpha_adaptinC2. 1 hit.
SM00288. VHS. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
SSF49348. SSF49348. 1 hit.
PROSITEiPS50180. GAE. 1 hit.
PS50909. GAT. 1 hit.
PS50179. VHS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGGA3_MOUSE
AccessioniPrimary (citable) accession number: Q8BMI3
Secondary accession number(s): Q3V1Y0, Q80U71
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: July 27, 2011
Last modified: September 7, 2016
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.