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Q8BMG7 (RBGPR_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 22, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Rab3 GTPase-activating protein non-catalytic subunit
Alternative name(s):
Rab3 GTPase-activating protein 150 kDa subunit
Rab3-GAP p150
Short name=Rab3-GAP150
Rab3-GAP regulatory subunit
Gene names
Name:Rab3gap2
Synonyms:Kiaa0839
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1366 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Regulatory subunit of a GTPase activating protein that has specificity for Rab3 subfamily (RAB3A, RAB3B, RAB3C and RAB3D). Rab3 proteins are involved in regulated exocytosis of neurotransmitters and hormones. Rab3 GTPase-activating complex specifically converts active Rab3-GTP to the inactive form Rab3-GDP. Required for normal eye and brain development. May participate in neurodevelopmental processes such as proliferation, migration and differentiation before synapse formation, and non-synaptic vesicular release of neurotransmitters By similarity.

Subunit structure

The Rab3 GTPase-activating complex is a heterodimer composed of RAB3GAP and RAB3-GAP150. The Rab3 GTPase-activating complex interacts with DMXL2 By similarity.

Subcellular location

Cytoplasm By similarity. Note: In neurons, it is enriched in the synaptic soluble fraction By similarity.

Sequence similarities

Belongs to the Rab3-GAP regulatory subunit family.

Sequence caution

The sequence AAH57872.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAC27296.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAC65667.2 differs from that shown. Reason: The sequence differs from that shown because it seems to be derived from a pre-mRNA.

Ontologies

Keywords
   Cellular componentCytoplasm
   Coding sequence diversityAlternative splicing
   Molecular functionGTPase activation
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processpositive regulation of GTPase activity

Inferred from electronic annotation. Source: GOC

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTPase activator activity

Inferred from electronic annotation. Source: UniProtKB-KW

enzyme regulator activity

Inferred from sequence or structural similarity. Source: UniProtKB

protein heterodimerization activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8BMG7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8BMG7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1068: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13661366Rab3 GTPase-activating protein non-catalytic subunit
PRO_0000191663

Amino acid modifications

Modified residue4481Phosphoserine By similarity
Modified residue8991Phosphothreonine By similarity

Natural variations

Alternative sequence1 – 10681068Missing in isoform 2.
VSP_013313

Experimental info

Sequence conflict7271I → V in BAC27296. Ref.1
Sequence conflict7471K → E in BAC27296. Ref.1
Sequence conflict7471K → E Ref.2
Sequence conflict8621E → V in BAC27296. Ref.1
Sequence conflict8621E → V Ref.2
Sequence conflict9061S → T in AAH57872. Ref.3
Sequence conflict10211Q → H in AAH57872. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified April 12, 2005. Version 2.
Checksum: A84C2C1416F673BA

FASTA1,366152,535
        10         20         30         40         50         60 
MACSIVQFCS FQDLQSARDF LFPHLREETP GALKRDPSKT SSWEDDSWGA WEETEPREPE 

        70         80         90        100        110        120 
EEGNTSKTQK NSWLQECVLS LSPTSDLMVI AREQKAAFLV RKWKHGDKGK EEMQFAVGWS 

       130        140        150        160        170        180 
GSVSAEEGEY VTSALCIPLA SQKRSSTGRP DWTCIVVGFT SGYVRFYTEG VLLLAQLLNE 

       190        200        210        220        230        240 
DKVLQLKCRT YEIPRHPGVT EQNEELSILY PAAIVTIDGF SLFQSLRACR NQVAKAAASG 

       250        260        270        280        290        300 
NENIQPPPLA YKKWGLQDID TIIDHASVGI MTLSPFDQMK TASNIGGFNA AIKNSPPAMS 

       310        320        330        340        350        360 
QYITVGSSPF TGFFYALEGS TQPLLSHVAL AVASKLTSAL FSAASGWLGW KSKHEEDTVQ 

       370        380        390        400        410        420 
KQKPKMEPAT PLAVRFGLPD SRRHGESICL SPCNTLAAVT DDFGRVILLD VARGIAIRMW 

       430        440        450        460        470        480 
KGYRDAQVGW IQIVEDLHER VPEKGGFSPF GNTQGPSRVA QFLVIYAPRR GILEVWNTQQ 

       490        500        510        520        530        540 
GPRVGAFNVG KHCRLLYPGY KIMGLNNVTS QSWQPQTYQI CLVDPVSASV KAVNVPFHLA 

       550        560        570        580        590        600 
LSDKKSERAK DLHLVKKLSA LLRAKSPRPD SFETEIKELI LDIKYPATKK QALESILASD 

       610        620        630        640        650        660 
RLSFSCLRNV TQTLMDTLKN QELESVDEGL LQFCASKLKL LHLYESVSQL NTLDFHSDTP 

       670        680        690        700        710        720 
FSDNDLAVLL RLDDKELLKL RALLEKYKQE NTKATVRFSE DADRVLPVKT FLEYLEYEKD 

       730        740        750        760        770        780 
ALSIRKIGEE ECVALGSFFF WKCLHGKSST EEMCHSLESA GLSPQQLLSL LLSVWLSKEK 

       790        800        810        820        830        840 
DILDKPQSVC CLHTMLSLLS KMKVAIDETW DSQSVSPWWQ QMRMACIQSE NSGAALLSAH 

       850        860        870        880        890        900 
VGHSVAAQMS SGATDKKFSQ MELDADAEAL TDSWEALSLD TEYWKLLLRQ LEDCLILQTL 

       910        920        930        940        950        960 
LHSKLSPPAA KAPSLQSEPL PRLSVKKLLE GGKGGIADSV AKWIFKQDLS PELLKCANKE 

       970        980        990       1000       1010       1020 
RDVENPDEPR EDLLHLAYEQ FPCSLELDVL HAHCCWEYVV QWNKDPEEAR FLVRSIEHLK 

      1030       1040       1050       1060       1070       1080 
QILNPHVQNG IALMMWNTFL VKRFSAATYL MDKVGKSPKD RLCRRDVGMS DTALTSFLGS 

      1090       1100       1110       1120       1130       1140 
CLELLQTSLE ADISRDEVQV PVLDTEDAWL SVEGPISIVE LALEQKPIHY PLVEHHSVLC 

      1150       1160       1170       1180       1190       1200 
SILYASMRFS LKSVKPLALF DSKGKNAFFK DLTSIQLLPS GEMDPNFISV RQQFLLKVVS 

      1210       1220       1230       1240       1250       1260 
AAVQAQHSKD KDPSAEAANT HWKDLNWPGL AVDLAHHLQV SDDVIRRHYV GELYSHGADL 

      1270       1280       1290       1300       1310       1320 
LGEEAIFQVQ DKEVLASQLL VLTGQRLAHA LFHTQTKEGM ELLARLPPTL CTWLKAMNPQ 

      1330       1340       1350       1360 
DLQNTGVPIA ATAKLVHKVM ELLPEKHGQY SLALHLIDAV EAMATL 

« Hide

Isoform 2 [UniParc].

Checksum: 1BC695910AC3D832
Show »

FASTA29832,950

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 319-1366 (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Cerebellum and Forelimb.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 283-1366 (ISOFORM 1).
Strain: NMRI.
Tissue: Mammary tumor.
[4]"Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 432-1364 (ISOFORM 1).
Tissue: Brain.
[5]Okazaki N., Kikuno R., Nagase T., Ohara O., Koga H.
Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[6]"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain cortex.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK031191 mRNA. Translation: BAC27296.1. Different initiation.
AK048759 mRNA. Translation: BAC33447.1.
AC129195 Genomic DNA. No translation available.
AC131084 Genomic DNA. No translation available.
AC158963 Genomic DNA. No translation available.
BC057872 mRNA. Translation: AAH57872.1. Different initiation.
AK122385 Transcribed RNA. Translation: BAC65667.2. Sequence problems.
RefSeqNP_001157226.1. NM_001163754.1.
UniGeneMm.275841.

3D structure databases

ProteinModelPortalQ8BMG7.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ8BMG7.

Proteomic databases

PaxDbQ8BMG7.
PRIDEQ8BMG7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID98732.
KEGGmmu:98732.

Organism-specific databases

CTD25782.
MGIMGI:1916043. Rab3gap2.
RougeSearch...

Phylogenomic databases

eggNOGNOG317114.
HOGENOMHOG000290717.
HOVERGENHBG067039.
InParanoidQ8BMG7.

Gene expression databases

CleanExMM_RAB3GAP2.
GenevestigatorQ8BMG7.

Family and domain databases

InterProIPR026059. Rab3-gap_reg.
[Graphical view]
PANTHERPTHR12472. PTHR12472. 1 hit.
ProtoNetSearch...

Other

ChiTaRSRAB3GAP2. mouse.
NextBio353601.
PROQ8BMG7.
SOURCESearch...

Entry information

Entry nameRBGPR_MOUSE
AccessionPrimary (citable) accession number: Q8BMG7
Secondary accession number(s): Q6PEU0, Q80TQ6, Q8BX72
Entry history
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: April 12, 2005
Last modified: January 22, 2014
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot