Q8BMG7 (RBGPR_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Rab3 GTPase-activating protein non-catalytic subunit

Alternative name(s):
Rab3 GTPase-activating protein 150 kDa subunit
Rab3-GAP p150
Short name=Rab3-GAP150
Rab3-GAP regulatory subunit

Gene names

Name:	Rab3gap2
Synonyms:	Kiaa0839

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1366 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulatory subunit of a GTPase activating protein that has specificity for Rab3 subfamily (RAB3A, RAB3B, RAB3C and RAB3D). Rab3 proteins are involved in regulated exocytosis of neurotransmitters and hormones. Rab3 GTPase-activating complex specifically converts active Rab3-GTP to the inactive form Rab3-GDP. Required for normal eye and brain development. May participate in neurodevelopmental processes such as proliferation, migration and differentiation before synapse formation, and non-synaptic vesicular release of neurotransmitters By similarity.
Subunit structure	The Rab3 GTPase-activating complex is a heterodimer composed of RAB3GAP and RAB3-GAP150. The Rab3 GTPase-activating complex interacts with DMXL2 By similarity.
Subcellular location	Cytoplasm By similarity. Note: In neurons, it is enriched in the synaptic soluble fraction By similarity.
Sequence similarities	Belongs to the Rab3-GAP regulatory subunit family.
Sequence caution	The sequence AAH57872.1 differs from that shown. Reason: Erroneous initiation. The sequence BAC27296.1 differs from that shown. Reason: Erroneous initiation. The sequence BAC65667.2 differs from that shown. Reason: The sequence differs from that shown because it seems to be derived from a pre-mRNA.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Molecular function	GTPase activation
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of GTPase activity Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTPase activator activity Inferred from electronic annotation. Source: UniProtKB-KW enzyme regulator activity Inferred from sequence or structural similarity. Source: UniProtKB protein heterodimerization activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8BMG7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8BMG7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1068: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1366	1366	Rab3 GTPase-activating protein non-catalytic subunit		PRO_0000191663
Amino acid modifications
	Modified residue	448	1	Phosphoserine Ref.6
	Modified residue	899	1	Phosphothreonine By similarity
Natural variations
	Alternative sequence	1 – 1068	1068	Missing in isoform 2.		VSP_013313
Experimental info
	Sequence conflict	727	1	I → V in BAC27296. Ref.1
	Sequence conflict	747	1	K → E in BAC27296. Ref.1
	Sequence conflict	747	1	K → E Ref.2
	Sequence conflict	862	1	E → V in BAC27296. Ref.1
	Sequence conflict	862	1	E → V Ref.2
	Sequence conflict	906	1	S → T in AAH57872. Ref.3
	Sequence conflict	1021	1	Q → H in AAH57872. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 12, 2005. Version 2. Checksum: A84C2C1416F673BA	FASTA	1,366	152,535
	10 20 30 40 50 60 MACSIVQFCS FQDLQSARDF LFPHLREETP GALKRDPSKT SSWEDDSWGA WEETEPREPE 70 80 90 100 110 120 EEGNTSKTQK NSWLQECVLS LSPTSDLMVI AREQKAAFLV RKWKHGDKGK EEMQFAVGWS 130 140 150 160 170 180 GSVSAEEGEY VTSALCIPLA SQKRSSTGRP DWTCIVVGFT SGYVRFYTEG VLLLAQLLNE 190 200 210 220 230 240 DKVLQLKCRT YEIPRHPGVT EQNEELSILY PAAIVTIDGF SLFQSLRACR NQVAKAAASG 250 260 270 280 290 300 NENIQPPPLA YKKWGLQDID TIIDHASVGI MTLSPFDQMK TASNIGGFNA AIKNSPPAMS 310 320 330 340 350 360 QYITVGSSPF TGFFYALEGS TQPLLSHVAL AVASKLTSAL FSAASGWLGW KSKHEEDTVQ 370 380 390 400 410 420 KQKPKMEPAT PLAVRFGLPD SRRHGESICL SPCNTLAAVT DDFGRVILLD VARGIAIRMW 430 440 450 460 470 480 KGYRDAQVGW IQIVEDLHER VPEKGGFSPF GNTQGPSRVA QFLVIYAPRR GILEVWNTQQ 490 500 510 520 530 540 GPRVGAFNVG KHCRLLYPGY KIMGLNNVTS QSWQPQTYQI CLVDPVSASV KAVNVPFHLA 550 560 570 580 590 600 LSDKKSERAK DLHLVKKLSA LLRAKSPRPD SFETEIKELI LDIKYPATKK QALESILASD 610 620 630 640 650 660 RLSFSCLRNV TQTLMDTLKN QELESVDEGL LQFCASKLKL LHLYESVSQL NTLDFHSDTP 670 680 690 700 710 720 FSDNDLAVLL RLDDKELLKL RALLEKYKQE NTKATVRFSE DADRVLPVKT FLEYLEYEKD 730 740 750 760 770 780 ALSIRKIGEE ECVALGSFFF WKCLHGKSST EEMCHSLESA GLSPQQLLSL LLSVWLSKEK 790 800 810 820 830 840 DILDKPQSVC CLHTMLSLLS KMKVAIDETW DSQSVSPWWQ QMRMACIQSE NSGAALLSAH 850 860 870 880 890 900 VGHSVAAQMS SGATDKKFSQ MELDADAEAL TDSWEALSLD TEYWKLLLRQ LEDCLILQTL 910 920 930 940 950 960 LHSKLSPPAA KAPSLQSEPL PRLSVKKLLE GGKGGIADSV AKWIFKQDLS PELLKCANKE 970 980 990 1000 1010 1020 RDVENPDEPR EDLLHLAYEQ FPCSLELDVL HAHCCWEYVV QWNKDPEEAR FLVRSIEHLK 1030 1040 1050 1060 1070 1080 QILNPHVQNG IALMMWNTFL VKRFSAATYL MDKVGKSPKD RLCRRDVGMS DTALTSFLGS 1090 1100 1110 1120 1130 1140 CLELLQTSLE ADISRDEVQV PVLDTEDAWL SVEGPISIVE LALEQKPIHY PLVEHHSVLC 1150 1160 1170 1180 1190 1200 SILYASMRFS LKSVKPLALF DSKGKNAFFK DLTSIQLLPS GEMDPNFISV RQQFLLKVVS 1210 1220 1230 1240 1250 1260 AAVQAQHSKD KDPSAEAANT HWKDLNWPGL AVDLAHHLQV SDDVIRRHYV GELYSHGADL 1270 1280 1290 1300 1310 1320 LGEEAIFQVQ DKEVLASQLL VLTGQRLAHA LFHTQTKEGM ELLARLPPTL CTWLKAMNPQ 1330 1340 1350 1360 DLQNTGVPIA ATAKLVHKVM ELLPEKHGQY SLALHLIDAV EAMATL
	Isoform 2 [UniParc]. Checksum: 1BC695910AC3D832	FASTA	298	32,950
	10 20 30 40 50 60 MSDTALTSFL GSCLELLQTS LEADISRDEV QVPVLDTEDA WLSVEGPISI VELALEQKPI 70 80 90 100 110 120 HYPLVEHHSV LCSILYASMR FSLKSVKPLA LFDSKGKNAF FKDLTSIQLL PSGEMDPNFI 130 140 150 160 170 180 SVRQQFLLKV VSAAVQAQHS KDKDPSAEAA NTHWKDLNWP GLAVDLAHHL QVSDDVIRRH 190 200 210 220 230 240 YVGELYSHGA DLLGEEAIFQ VQDKEVLASQ LLVLTGQRLA HALFHTQTKE GMELLARLPP 250 260 270 280 290 TLCTWLKAMN PQDLQNTGVP IAATAKLVHK VMELLPEKHG QYSLALHLID AVEAMATL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 319-1366 (ISOFORM 1). Strain: C57BL/6J. Tissue: Cerebellum and Forelimb.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 283-1366 (ISOFORM 1). Strain: NMRI. Tissue: Mammary tumor.
[4]	"Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H. DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 432-1364 (ISOFORM 1). Tissue: Brain.
[5]	Okazaki N., Kikuno R., Nagase T., Ohara O., Koga H. Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[6]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-448, MASS SPECTROMETRY. Tissue: Brain cortex.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK031191 mRNA. Translation: BAC27296.1. Different initiation. AK048759 mRNA. Translation: BAC33447.1. AC129195 Genomic DNA. No translation available. AC131084 Genomic DNA. No translation available. AC158963 Genomic DNA. No translation available. BC057872 mRNA. Translation: AAH57872.1. Different initiation. AK122385 Transcribed RNA. Translation: BAC65667.2. Sequence problems.
IPI	IPI00555130. IPI00944792.
RefSeq	NP_001157226.1. NM_001163754.1.
UniGene	Mm.275841.
3D structure databases
ProteinModelPortal	Q8BMG7.
ModBase	Search...
PTM databases
PhosphoSite	Q8BMG7.
Proteomic databases
PaxDb	Q8BMG7.
PRIDE	Q8BMG7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	98732.
KEGG	mmu:98732.
UCSC	uc007dyu.2. mouse.
Organism-specific databases
CTD	25782.
MGI	MGI:1916043. Rab3gap2.
Rouge	Search...
Phylogenomic databases
eggNOG	NOG317114.
HOGENOM	HOG000290717.
HOVERGEN	HBG067039.
InParanoid	Q8BMG7.
OrthoDB	EOG470TGK.
Gene expression databases
CleanEx	MM_RAB3GAP2.
Genevestigator	Q8BMG7.
GermOnline	ENSMUSG00000039318. Mus musculus.
Family and domain databases
InterPro	IPR026059. Rab3-gap_reg. [Graphical view]
PANTHER	PTHR12472. PTHR12472. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	RAB3GAP2. mouse.
NextBio	353601.
SOURCE	Search...

Entry information

Entry name

RBGPR_MOUSE

Accession

Primary (citable) accession number: Q8BMG7
Secondary accession number(s): Q6PEU0, Q80TQ6, Q8BX72

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 12, 2005
Last sequence update:	April 12, 2005
Last modified:	April 3, 2013
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program