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Protein

NADP-dependent malic enzyme, mitochondrial

Gene

Me3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-malate + NADP+ = pyruvate + CO2 + NADPH.
Oxaloacetate = pyruvate + CO2.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Divalent metal cations. Prefers magnesium or manganese.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei137Proton donorBy similarity1
Binding sitei190NADPBy similarity1
Active sitei208Proton acceptorBy similarity1
Metal bindingi280Divalent metal cationBy similarity1
Metal bindingi281Divalent metal cationBy similarity1
Metal bindingi304Divalent metal cationBy similarity1
Binding sitei304NADPBy similarity1
Sitei304Important for activityBy similarity1
Binding sitei443NADPBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
NADP-dependent malic enzyme, mitochondrial (EC:1.1.1.40)
Short name:
NADP-ME
Alternative name(s):
Malic enzyme 3
Gene namesi
Name:Me3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1916679. Me3.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: UniProtKB-SubCell
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000018540? – 604NADP-dependent malic enzyme, mitochondrial
Transit peptidei1 – ?MitochondrionSequence analysis

Proteomic databases

MaxQBiQ8BMF3.
PaxDbiQ8BMF3.
PeptideAtlasiQ8BMF3.
PRIDEiQ8BMF3.

PTM databases

iPTMnetiQ8BMF3.
PhosphoSitePlusiQ8BMF3.
SwissPalmiQ8BMF3.

Expressioni

Gene expression databases

BgeeiENSMUSG00000030621.
CleanExiMM_ME3.
ExpressionAtlasiQ8BMF3. baseline and differential.
GenevisibleiQ8BMF3. MM.

Interactioni

Protein-protein interaction databases

IntActiQ8BMF3. 1 interactor.
MINTiMINT-4111524.
STRINGi10090.ENSMUSP00000032856.

Structurei

3D structure databases

ProteinModelPortaliQ8BMF3.
SMRiQ8BMF3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the malic enzymes family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1257. Eukaryota.
COG0281. LUCA.
GeneTreeiENSGT00390000000754.
HOGENOMiHOG000042486.
HOVERGENiHBG000746.
InParanoidiQ8BMF3.
KOiK00029.
OMAiCYQVTEG.
OrthoDBiEOG091G04H9.
TreeFamiTF300537.

Family and domain databases

Gene3Di3.40.50.10380. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR015884. Malic_enzyme_CS.
IPR012301. Malic_N_dom.
IPR012302. Malic_NAD-bd.
IPR001891. Malic_OxRdtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00390. malic. 1 hit.
PF03949. Malic_M. 1 hit.
[Graphical view]
PIRSFiPIRSF000106. ME. 1 hit.
PRINTSiPR00072. MALOXRDTASE.
SMARTiSM01274. malic. 1 hit.
SM00919. Malic_M. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00331. MALIC_ENZYMES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8BMF3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGAALGTGAR LTSVPRIACS SLRRQAPSAP AQGCHSKSGP PRPVPLKKRG
60 70 80 90 100
YDVTRNPHLN KGMAFTLEER LQLGIHGLIP PCFLSQDVQL LRIMRYYENQ
110 120 130 140 150
QSDLDKYIIL MTLQDRNEKL FYRVLTSDVE KFMPIVYTPT VGLACQHYGL
160 170 180 190 200
TFRRPRGLFI TIHDKGHIAT MLNSWPEDNI KAVVVTDGER ILGLGDLGCY
210 220 230 240 250
GMGIPVGKLA LYTACGGVNP QQCLPVLLDV GTNNEELLRD PLYIGLKHQR
260 270 280 290 300
VRGEEYDDLL DEFMQAVTDK FGINCLIQFE DFANANAFRL LNKYRNKYCM
310 320 330 340 350
FNDDIQGTAS VAVAGILAAL RITKNRLSNH VFVFQGAGEA AMGIAHLLVM
360 370 380 390 400
ALEKEGIPKT EAIKKIWMVD SKGLIVKGRS HLNHEKEMFA QDHPEVNSLE
410 420 430 440 450
EVVRLVKPTA IIGVAAIAGA FTEQILRDMA SFHERPIVFA LSNPTSKAEC
460 470 480 490 500
TAEKCYRVTE GRGIFASGSP FKSVTLEDGR TFTPGQGNNA YVFPGVALGV
510 520 530 540 550
IAGGIRHIPD EIFLLTAEQI AQEVSEQHLS QGRLYPPLST IRDVSLRIAV
560 570 580 590 600
KVLDYAYKHN LASYYPEPKD KEAFVKSLIY TPDYDSFSLD TYSWPKEAMS

VQKV
Length:604
Mass (Da):67,098
Last modified:July 27, 2011 - v2
Checksum:iF667B01B116A5918
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti113L → H in BAC27751 (PubMed:16141072).Curated1
Sequence conflicti338G → D in BAC27751 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK032196 mRNA. Translation: BAC27751.1.
BC099935 mRNA. Translation: AAH99935.1.
CCDSiCCDS21443.1.
RefSeqiNP_852072.2. NM_181407.4.
XP_006507252.1. XM_006507189.3.
XP_006507253.1. XM_006507190.3.
UniGeneiMm.334011.

Genome annotation databases

EnsembliENSMUST00000032856; ENSMUSP00000032856; ENSMUSG00000030621.
GeneIDi109264.
KEGGimmu:109264.
UCSCiuc009igd.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK032196 mRNA. Translation: BAC27751.1.
BC099935 mRNA. Translation: AAH99935.1.
CCDSiCCDS21443.1.
RefSeqiNP_852072.2. NM_181407.4.
XP_006507252.1. XM_006507189.3.
XP_006507253.1. XM_006507190.3.
UniGeneiMm.334011.

3D structure databases

ProteinModelPortaliQ8BMF3.
SMRiQ8BMF3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BMF3. 1 interactor.
MINTiMINT-4111524.
STRINGi10090.ENSMUSP00000032856.

PTM databases

iPTMnetiQ8BMF3.
PhosphoSitePlusiQ8BMF3.
SwissPalmiQ8BMF3.

Proteomic databases

MaxQBiQ8BMF3.
PaxDbiQ8BMF3.
PeptideAtlasiQ8BMF3.
PRIDEiQ8BMF3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032856; ENSMUSP00000032856; ENSMUSG00000030621.
GeneIDi109264.
KEGGimmu:109264.
UCSCiuc009igd.1. mouse.

Organism-specific databases

CTDi10873.
MGIiMGI:1916679. Me3.

Phylogenomic databases

eggNOGiKOG1257. Eukaryota.
COG0281. LUCA.
GeneTreeiENSGT00390000000754.
HOGENOMiHOG000042486.
HOVERGENiHBG000746.
InParanoidiQ8BMF3.
KOiK00029.
OMAiCYQVTEG.
OrthoDBiEOG091G04H9.
TreeFamiTF300537.

Miscellaneous databases

PROiQ8BMF3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030621.
CleanExiMM_ME3.
ExpressionAtlasiQ8BMF3. baseline and differential.
GenevisibleiQ8BMF3. MM.

Family and domain databases

Gene3Di3.40.50.10380. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR015884. Malic_enzyme_CS.
IPR012301. Malic_N_dom.
IPR012302. Malic_NAD-bd.
IPR001891. Malic_OxRdtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00390. malic. 1 hit.
PF03949. Malic_M. 1 hit.
[Graphical view]
PIRSFiPIRSF000106. ME. 1 hit.
PRINTSiPR00072. MALOXRDTASE.
SMARTiSM01274. malic. 1 hit.
SM00919. Malic_M. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00331. MALIC_ENZYMES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMAON_MOUSE
AccessioniPrimary (citable) accession number: Q8BMF3
Secondary accession number(s): Q499F4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.