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Q8BMA5

- NPAT_MOUSE

UniProt

Q8BMA5 - NPAT_MOUSE

Protein

Protein NPAT

Gene

Npat

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 2 (05 Feb 2008)
      Previous versions | rss
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    Functioni

    Required for progression through the G1 and S phases of the cell cycle and for S phase entry. Activates transcription of the histone H2A, histone H2B, histone H3 and histone H4 genes in conjunction with MIZF. Also positively regulates the ATM, MIZF and PRKDC promoters. Transcriptional activation may be accomplished at least in part by the recruitment of the NuA4 histone acetyltransferase (HAT) complex to target gene promoters By similarity. Required for early embryonic development.By similarity1 Publication

    GO - Molecular functioni

    1. sequence-specific DNA binding transcription factor activity Source: Ensembl
    2. transcription coactivator activity Source: UniProtKB
    3. transcription corepressor activity Source: Ensembl

    GO - Biological processi

    1. positive regulation of transcription, DNA-templated Source: Ensembl
    2. regulation of transcription involved in G1/S transition of mitotic cell cycle Source: Ensembl
    3. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Cell cycle, Transcription, Transcription regulation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein NPAT
    Gene namesi
    Name:Npat
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:107605. Npat.

    Subcellular locationi

    Nucleus 1 Publication
    Note: Concentrates in Cajal bodies tethered to histone gene clusters.By similarity

    GO - Cellular componenti

    1. Cajal body Source: UniProtKB
    2. cytoplasm Source: UniProtKB
    3. Gemini of coiled bodies Source: UniProtKB
    4. nucleoplasm Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 14201420Protein NPATPRO_0000318164Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei771 – 7711Phosphoserine; by CDK2By similarity
    Modified residuei773 – 7731Phosphoserine; by CDK2By similarity
    Modified residuei1096 – 10961Phosphoserine; by CDK2By similarity
    Modified residuei1223 – 12231N6-acetyllysineBy similarity
    Modified residuei1264 – 12641Phosphothreonine; by CDK2By similarity
    Modified residuei1343 – 13431Phosphothreonine; by CDK2By similarity

    Post-translational modificationi

    Phosphorylated at Ser-771, Ser-773, Ser-1096, Thr-1264 and Thr-1343 by CCNE1/CDK2 at G1-S transition and until prophase, which promotes association with histone gene clusters and stimulates activation of histone transcription. Also phosphorylated by CCNA1/CDK2 in vitro By similarity.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PRIDEiQ8BMA5.

    PTM databases

    PhosphoSiteiQ8BMA5.

    Expressioni

    Developmental stagei

    Expression peaks at the G1/S phase boundary.1 Publication

    Gene expression databases

    BgeeiQ8BMA5.
    CleanExiMM_NPAT.
    GenevestigatoriQ8BMA5.

    Interactioni

    Subunit structurei

    Interacts with the cylin/CDK complexes CCNE1/CDK2 and CCNA1/CDK2. Interacts with BZW1, CASP8AP2, CREBBP, MIZF and YY1. Interacts with the RUVBL1, RUVBL2 and TRRAP subunits of the NuA4 complex. May also interact with GAPDH, NME1, NME2 and STIP1 By similarity.By similarity

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 3533LisHPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 319319Interaction with MIZFBy similarityAdd
    BLAST
    Regioni5 – 2521Required for activation of histone gene transcription and interaction with MIZFBy similarityAdd
    BLAST
    Regioni121 – 14525Required for activation of histone gene transcription and interaction with MIZFBy similarityAdd
    BLAST
    Regioni263 – 33977Mediates transcriptional activationBy similarityAdd
    BLAST
    Regioni628 – 65225Required for acceleration of G1 phaseBy similarityAdd
    BLAST
    Regioni822 – 84726Required for acceleration of G1 phaseBy similarityAdd
    BLAST
    Regioni1033 – 104816Required for acceleration of G1 phaseBy similarityAdd
    BLAST
    Regioni1223 – 124725Required for acceleration of G1 phaseBy similarityAdd
    BLAST
    Regioni1319 – 134224Required for acceleration of G1 phaseBy similarityAdd
    BLAST

    Domaini

    The LisH domain is required for the activation of histone gene transcription.By similarity

    Sequence similaritiesi

    Belongs to the NPAT family.Curated
    Contains 1 LisH domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG29266.
    GeneTreeiENSGT00390000012388.
    HOGENOMiHOG000113840.
    HOVERGENiHBG108198.
    InParanoidiQ8BMA5.
    OMAiFDLFDYG.
    OrthoDBiEOG7JDQX0.
    PhylomeDBiQ8BMA5.
    TreeFamiTF332825.

    Family and domain databases

    InterProiIPR006594. LisH_dimerisation.
    [Graphical view]
    SMARTiSM00667. LisH. 1 hit.
    [Graphical view]
    PROSITEiPS50896. LISH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8BMA5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLLPSDVARL VLGYLQQENL TSTCQTFILE SSNLKEYAEH CTDEGFIPAC     50
    LLSLFGKNLT TILNEYVAMK AKETSNDVPT IMSSLWKKLD HTLSQIRSMH 100
    SSPGFAAHQR ARTRNGIAEI KRQRWLASQA APVSSELLVL PYASGQFTTS 150
    PLVATQAVKP TGPISTPVRS NIVVVNQSQP QSTVTNTAGE SLNIIPGPQE 200
    RKTQTSLMSP GRRKSESQKK SLTSSGPHSS RNFQDPNAFA VEKQMVIENA 250
    REKILSNKSL QEKLAENINK FLTSDSSVAQ VPKQTDSNPT EPETSIDELL 300
    GLPSEIHMSE EAIQDILEQT ESDPAFQALF DLFDYGKTKN NKNMPQISSQ 350
    PMETNSNIVL PEETNLTIKS SFETEESDGQ SGQPPFCTSY QNEDVLLNDL 400
    KSGNSHDVLP QESQENFSQI SSNIQKKTFK TAIPAEQKCA LDITLESVSN 450
    LSDFNQRGSS AECNEHCSEL FASQIPTEAE VAVGEKNSLS ADILSQSQYQ 500
    PDQPSVPVTS FVSLGGETND KNLVLSGKNS QLLSQSTPLT TKPSKSQLCE 550
    NSNNIIKVKT NPQASESADS SETANRKTET NTVSPAAAQP QADCQDNSPL 600
    QSKPPPGIGE SLGVNVTEKI EIHLEEPAPS DKQLSNDAAS VDLNPTESKT 650
    EPLQSASAQE PEPPSVKDGD TIFLSLSEHN SCEEVALVLG EGNPVKNNNS 700
    LSSESGGSVG VSPETQNTDG KTSNSTEVDA SSIVSLKIII SDDPFVSSDA 750
    ELNSAVSSIS GENLPTIILS SKSPAKNAEF VTCLSSEETA SAVVSVEVGD 800
    SGSMEQNLLV LKPEEPMVNN TQNEDGIAFS ANVAPCVPKD GGYIQLMPTT 850
    STAFGNSSNI LIATCMTDST ALGPTVSQSN VVVLPGSSAP MTAQPPQQQL 900
    QTPPKSNSAF AVSQAVSPNF SQGSAIIIAS PVQPVLQGMV GMIPVSVVGQ 950
    NGNTFSTPPQ QVLHMPLAAP VCNRSIAQLP IPQKSQKAQG LRNKLITGKQ 1000
    VNNLTNLSSL SEACHTQRTE ASDKNIATEL GKKMEDTTIS LSGERVAPPS 1050
    KPFESHRRVL CFDSTVSSVA NTQGSLYKMT SENKEKKEAS FSHLDSPILS 1100
    STLKPPPNNA IKREREKTVP KILSKSETAS SRHTTVKEVQ SEKKVSPTEV 1150
    ALESLHKATA NKENELCGDG ERPKNADTSK LPGGQQNGSL RNEKAIASLQ 1200
    ELTKKQATPS NNKNATSVGG TVKDQKQEQS KPASSLIGAE ILQDVPIHSP 1250
    ANRSADTDLP VPRTPGSGAG EKHKEEPSDS MKAPASRRCG EEGSMPRVMI 1300
    PPVTADLPAC SPASETGSEN SVSMAAHTLM ILSRAAIART TATPLKDNTQ 1350
    QFRTSSRSTT KKRKIEELDE CERNSRTSGK NLANSSVPMK KKKIKKKKLP 1400
    SSFPAGMDVD KFLLSLHYDE 1420
    Length:1,420
    Mass (Da):152,176
    Last modified:February 5, 2008 - v2
    Checksum:i1035459489343314
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1141 – 11411S → P in BAC35255. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK033012 mRNA. Translation: BAC28125.1.
    AK042624 mRNA. Translation: BAC31308.1.
    AK053074 mRNA. Translation: BAC35255.1.
    AC156640 Genomic DNA. No translation available.
    CCDSiCCDS40637.1.
    RefSeqiNP_001074621.1. NM_001081152.1.
    UniGeneiMm.116713.

    Genome annotation databases

    EnsembliENSMUST00000035850; ENSMUSP00000048709; ENSMUSG00000033054.
    GeneIDi244879.
    KEGGimmu:244879.
    UCSCiuc009pmf.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK033012 mRNA. Translation: BAC28125.1 .
    AK042624 mRNA. Translation: BAC31308.1 .
    AK053074 mRNA. Translation: BAC35255.1 .
    AC156640 Genomic DNA. No translation available.
    CCDSi CCDS40637.1.
    RefSeqi NP_001074621.1. NM_001081152.1.
    UniGenei Mm.116713.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q8BMA5.

    Proteomic databases

    PRIDEi Q8BMA5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000035850 ; ENSMUSP00000048709 ; ENSMUSG00000033054 .
    GeneIDi 244879.
    KEGGi mmu:244879.
    UCSCi uc009pmf.1. mouse.

    Organism-specific databases

    CTDi 4863.
    MGIi MGI:107605. Npat.

    Phylogenomic databases

    eggNOGi NOG29266.
    GeneTreei ENSGT00390000012388.
    HOGENOMi HOG000113840.
    HOVERGENi HBG108198.
    InParanoidi Q8BMA5.
    OMAi FDLFDYG.
    OrthoDBi EOG7JDQX0.
    PhylomeDBi Q8BMA5.
    TreeFami TF332825.

    Miscellaneous databases

    NextBioi 386460.
    PROi Q8BMA5.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8BMA5.
    CleanExi MM_NPAT.
    Genevestigatori Q8BMA5.

    Family and domain databases

    InterProi IPR006594. LisH_dimerisation.
    [Graphical view ]
    SMARTi SM00667. LisH. 1 hit.
    [Graphical view ]
    PROSITEi PS50896. LISH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Cerebellum, Head and Muellerian duct.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "Proviral inactivation of the Npat gene of Mpv 20 mice results in early embryonic arrest."
      Di Fruscio M., Weiher H., Vanderhyden B.C., Imai T., Shiomi T., Hori T., Jaenisch R., Gray D.A.
      Mol. Cell. Biol. 17:4080-4086(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "NPAT expression is regulated by E2F and is essential for cell cycle progression."
      Gao G., Bracken A.P., Burkard K., Pasini D., Classon M., Attwooll C., Sagara M., Imai T., Helin K., Zhao J.
      Mol. Cell. Biol. 23:2821-2833(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.
    5. Cited for: SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiNPAT_MOUSE
    AccessioniPrimary (citable) accession number: Q8BMA5
    Secondary accession number(s): Q8BWA9, Q8BY06
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: February 5, 2008
    Last modified: October 1, 2014
    This is version 75 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3