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Q8BMA5

- NPAT_MOUSE

UniProt

Q8BMA5 - NPAT_MOUSE

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Protein
Protein NPAT
Gene
Npat
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Required for progression through the G1 and S phases of the cell cycle and for S phase entry. Activates transcription of the histone H2A, histone H2B, histone H3 and histone H4 genes in conjunction with MIZF. Also positively regulates the ATM, MIZF and PRKDC promoters. Transcriptional activation may be accomplished at least in part by the recruitment of the NuA4 histone acetyltransferase (HAT) complex to target gene promoters By similarity. Required for early embryonic development.1 Publication

GO - Molecular functioni

  1. sequence-specific DNA binding transcription factor activity Source: Ensembl
  2. transcription coactivator activity Source: UniProtKB
  3. transcription corepressor activity Source: Ensembl
Complete GO annotation...

GO - Biological processi

  1. positive regulation of transcription, DNA-templated Source: Ensembl
  2. regulation of transcription involved in G1/S transition of mitotic cell cycle Source: Ensembl
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Cell cycle, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Protein NPAT
Gene namesi
Name:Npat
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:107605. Npat.

Subcellular locationi

Nucleus
Note: Concentrates in Cajal bodies tethered to histone gene clusters By similarity.1 Publication

GO - Cellular componenti

  1. Cajal body Source: UniProtKB
  2. Gemini of coiled bodies Source: UniProtKB
  3. cytoplasm Source: UniProtKB
  4. nucleoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14201420Protein NPAT
PRO_0000318164Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei771 – 7711Phosphoserine; by CDK2 By similarity
Modified residuei773 – 7731Phosphoserine; by CDK2 By similarity
Modified residuei1096 – 10961Phosphoserine; by CDK2 By similarity
Modified residuei1223 – 12231N6-acetyllysine By similarity
Modified residuei1264 – 12641Phosphothreonine; by CDK2 By similarity
Modified residuei1343 – 13431Phosphothreonine; by CDK2 By similarity

Post-translational modificationi

Phosphorylated at Ser-771, Ser-773, Ser-1096, Thr-1264 and Thr-1343 by CCNE1/CDK2 at G1-S transition and until prophase, which promotes association with histone gene clusters and stimulates activation of histone transcription. Also phosphorylated by CCNA1/CDK2 in vitro By similarity.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiQ8BMA5.

PTM databases

PhosphoSiteiQ8BMA5.

Expressioni

Developmental stagei

Expression peaks at the G1/S phase boundary.1 Publication

Gene expression databases

BgeeiQ8BMA5.
CleanExiMM_NPAT.
GenevestigatoriQ8BMA5.

Interactioni

Subunit structurei

Interacts with the cylin/CDK complexes CCNE1/CDK2 and CCNA1/CDK2. Interacts with BZW1, CASP8AP2, CREBBP, MIZF and YY1. Interacts with the RUVBL1, RUVBL2 and TRRAP subunits of the NuA4 complex. May also interact with GAPDH, NME1, NME2 and STIP1 By similarity.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 3533LisH
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 319319Interaction with MIZF By similarity
Add
BLAST
Regioni5 – 2521Required for activation of histone gene transcription and interaction with MIZF By similarity
Add
BLAST
Regioni121 – 14525Required for activation of histone gene transcription and interaction with MIZF By similarity
Add
BLAST
Regioni263 – 33977Mediates transcriptional activation By similarity
Add
BLAST
Regioni628 – 65225Required for acceleration of G1 phase By similarity
Add
BLAST
Regioni822 – 84726Required for acceleration of G1 phase By similarity
Add
BLAST
Regioni1033 – 104816Required for acceleration of G1 phase By similarity
Add
BLAST
Regioni1223 – 124725Required for acceleration of G1 phase By similarity
Add
BLAST
Regioni1319 – 134224Required for acceleration of G1 phase By similarity
Add
BLAST

Domaini

The LisH domain is required for the activation of histone gene transcription By similarity.

Sequence similaritiesi

Belongs to the NPAT family.
Contains 1 LisH domain.

Phylogenomic databases

eggNOGiNOG29266.
GeneTreeiENSGT00390000012388.
HOGENOMiHOG000113840.
HOVERGENiHBG108198.
InParanoidiQ8BMA5.
OMAiFDLFDYG.
OrthoDBiEOG7JDQX0.
PhylomeDBiQ8BMA5.
TreeFamiTF332825.

Family and domain databases

InterProiIPR006594. LisH_dimerisation.
[Graphical view]
SMARTiSM00667. LisH. 1 hit.
[Graphical view]
PROSITEiPS50896. LISH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BMA5-1 [UniParc]FASTAAdd to Basket

« Hide

MLLPSDVARL VLGYLQQENL TSTCQTFILE SSNLKEYAEH CTDEGFIPAC     50
LLSLFGKNLT TILNEYVAMK AKETSNDVPT IMSSLWKKLD HTLSQIRSMH 100
SSPGFAAHQR ARTRNGIAEI KRQRWLASQA APVSSELLVL PYASGQFTTS 150
PLVATQAVKP TGPISTPVRS NIVVVNQSQP QSTVTNTAGE SLNIIPGPQE 200
RKTQTSLMSP GRRKSESQKK SLTSSGPHSS RNFQDPNAFA VEKQMVIENA 250
REKILSNKSL QEKLAENINK FLTSDSSVAQ VPKQTDSNPT EPETSIDELL 300
GLPSEIHMSE EAIQDILEQT ESDPAFQALF DLFDYGKTKN NKNMPQISSQ 350
PMETNSNIVL PEETNLTIKS SFETEESDGQ SGQPPFCTSY QNEDVLLNDL 400
KSGNSHDVLP QESQENFSQI SSNIQKKTFK TAIPAEQKCA LDITLESVSN 450
LSDFNQRGSS AECNEHCSEL FASQIPTEAE VAVGEKNSLS ADILSQSQYQ 500
PDQPSVPVTS FVSLGGETND KNLVLSGKNS QLLSQSTPLT TKPSKSQLCE 550
NSNNIIKVKT NPQASESADS SETANRKTET NTVSPAAAQP QADCQDNSPL 600
QSKPPPGIGE SLGVNVTEKI EIHLEEPAPS DKQLSNDAAS VDLNPTESKT 650
EPLQSASAQE PEPPSVKDGD TIFLSLSEHN SCEEVALVLG EGNPVKNNNS 700
LSSESGGSVG VSPETQNTDG KTSNSTEVDA SSIVSLKIII SDDPFVSSDA 750
ELNSAVSSIS GENLPTIILS SKSPAKNAEF VTCLSSEETA SAVVSVEVGD 800
SGSMEQNLLV LKPEEPMVNN TQNEDGIAFS ANVAPCVPKD GGYIQLMPTT 850
STAFGNSSNI LIATCMTDST ALGPTVSQSN VVVLPGSSAP MTAQPPQQQL 900
QTPPKSNSAF AVSQAVSPNF SQGSAIIIAS PVQPVLQGMV GMIPVSVVGQ 950
NGNTFSTPPQ QVLHMPLAAP VCNRSIAQLP IPQKSQKAQG LRNKLITGKQ 1000
VNNLTNLSSL SEACHTQRTE ASDKNIATEL GKKMEDTTIS LSGERVAPPS 1050
KPFESHRRVL CFDSTVSSVA NTQGSLYKMT SENKEKKEAS FSHLDSPILS 1100
STLKPPPNNA IKREREKTVP KILSKSETAS SRHTTVKEVQ SEKKVSPTEV 1150
ALESLHKATA NKENELCGDG ERPKNADTSK LPGGQQNGSL RNEKAIASLQ 1200
ELTKKQATPS NNKNATSVGG TVKDQKQEQS KPASSLIGAE ILQDVPIHSP 1250
ANRSADTDLP VPRTPGSGAG EKHKEEPSDS MKAPASRRCG EEGSMPRVMI 1300
PPVTADLPAC SPASETGSEN SVSMAAHTLM ILSRAAIART TATPLKDNTQ 1350
QFRTSSRSTT KKRKIEELDE CERNSRTSGK NLANSSVPMK KKKIKKKKLP 1400
SSFPAGMDVD KFLLSLHYDE 1420
Length:1,420
Mass (Da):152,176
Last modified:February 5, 2008 - v2
Checksum:i1035459489343314
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1141 – 11411S → P in BAC35255. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK033012 mRNA. Translation: BAC28125.1.
AK042624 mRNA. Translation: BAC31308.1.
AK053074 mRNA. Translation: BAC35255.1.
AC156640 Genomic DNA. No translation available.
CCDSiCCDS40637.1.
RefSeqiNP_001074621.1. NM_001081152.1.
UniGeneiMm.116713.

Genome annotation databases

EnsembliENSMUST00000035850; ENSMUSP00000048709; ENSMUSG00000033054.
GeneIDi244879.
KEGGimmu:244879.
UCSCiuc009pmf.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK033012 mRNA. Translation: BAC28125.1 .
AK042624 mRNA. Translation: BAC31308.1 .
AK053074 mRNA. Translation: BAC35255.1 .
AC156640 Genomic DNA. No translation available.
CCDSi CCDS40637.1.
RefSeqi NP_001074621.1. NM_001081152.1.
UniGenei Mm.116713.

3D structure databases

ModBasei Search...

PTM databases

PhosphoSitei Q8BMA5.

Proteomic databases

PRIDEi Q8BMA5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000035850 ; ENSMUSP00000048709 ; ENSMUSG00000033054 .
GeneIDi 244879.
KEGGi mmu:244879.
UCSCi uc009pmf.1. mouse.

Organism-specific databases

CTDi 4863.
MGIi MGI:107605. Npat.

Phylogenomic databases

eggNOGi NOG29266.
GeneTreei ENSGT00390000012388.
HOGENOMi HOG000113840.
HOVERGENi HBG108198.
InParanoidi Q8BMA5.
OMAi FDLFDYG.
OrthoDBi EOG7JDQX0.
PhylomeDBi Q8BMA5.
TreeFami TF332825.

Miscellaneous databases

NextBioi 386460.
PROi Q8BMA5.
SOURCEi Search...

Gene expression databases

Bgeei Q8BMA5.
CleanExi MM_NPAT.
Genevestigatori Q8BMA5.

Family and domain databases

InterProi IPR006594. LisH_dimerisation.
[Graphical view ]
SMARTi SM00667. LisH. 1 hit.
[Graphical view ]
PROSITEi PS50896. LISH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Cerebellum, Head and Muellerian duct.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "Proviral inactivation of the Npat gene of Mpv 20 mice results in early embryonic arrest."
    Di Fruscio M., Weiher H., Vanderhyden B.C., Imai T., Shiomi T., Hori T., Jaenisch R., Gray D.A.
    Mol. Cell. Biol. 17:4080-4086(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "NPAT expression is regulated by E2F and is essential for cell cycle progression."
    Gao G., Bracken A.P., Burkard K., Pasini D., Classon M., Attwooll C., Sagara M., Imai T., Helin K., Zhao J.
    Mol. Cell. Biol. 23:2821-2833(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  5. Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiNPAT_MOUSE
AccessioniPrimary (citable) accession number: Q8BMA5
Secondary accession number(s): Q8BWA9, Q8BY06
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 5, 2008
Last modified: July 9, 2014
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3
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