Q8BMA5 (NPAT_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein NPAT

Gene names

Name:

Npat

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1420 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Required for progression through the G1 and S phases of the cell cycle and for S phase entry. Activates transcription of the histone H2A, histone H2B, histone H3 and histone H4 genes in conjunction with MIZF. Also positively regulates the ATM, MIZF and PRKDC promoters. Transcriptional activation may be accomplished at least in part by the recruitment of the NuA4 histone acetyltransferase (HAT) complex to target gene promoters By similarity. Required for early embryonic development. Ref.3
Subunit structure	Interacts with the cylin/CDK complexes CCNE1/CDK2 and CCNA1/CDK2. Interacts with BZW1, CASP8AP2, CREBBP, MIZF and YY1. Interacts with the RUVBL1, RUVBL2 and TRRAP subunits of the NuA4 complex. May also interact with GAPDH, NME1, NME2 and STIP1 By similarity.
Subcellular location	Nucleus. Note: Concentrates in Cajal bodies tethered to histone gene clusters By similarity. Ref.5
Developmental stage	Expression peaks at the G1/S phase boundary. Ref.4
Domain	The LisH domain is required for the activation of histone gene transcription By similarity.
Post-translational modification	Phosphorylated at Ser-771, Ser-773, Ser-1096, Thr-1264 and Thr-1343 by CCNE1/CDK2 at G1-S transition and until prophase, which promotes association with histone gene clusters and stimulates activation of histone transcription. Also phosphorylated by CCNA1/CDK2 in vitro By similarity.
Sequence similarities	Belongs to the NPAT family. Contains 1 LisH domain.

Ontologies

Keywords
Biological process	Cell cycle Transcription Transcription regulation
Cellular component	Nucleus
Molecular function	Activator
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: Compara regulation of transcription involved in G1/S phase of mitotic cell cycle Inferred from electronic annotation. Source: Compara transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Cajal body Inferred from electronic annotation. Source: Compara
Molecular_function	sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: Compara transcription coactivator activity Inferred from sequence or structural similarity. Source: UniProtKB transcription corepressor activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1420	1420	Protein NPAT		PRO_0000318164
Regions
	Domain	3 – 35	33	LisH
	Region	1 – 319	319	Interaction with MIZF By similarity
	Region	5 – 25	21	Required for activation of histone gene transcription and interaction with MIZF By similarity
	Region	121 – 145	25	Required for activation of histone gene transcription and interaction with MIZF By similarity
	Region	263 – 339	77	Mediates transcriptional activation By similarity
	Region	628 – 652	25	Required for acceleration of G1 phase By similarity
	Region	822 – 847	26	Required for acceleration of G1 phase By similarity
	Region	1033 – 1048	16	Required for acceleration of G1 phase By similarity
	Region	1223 – 1247	25	Required for acceleration of G1 phase By similarity
	Region	1319 – 1342	24	Required for acceleration of G1 phase By similarity
Amino acid modifications
	Modified residue	771	1	Phosphoserine; by CDK2 By similarity
	Modified residue	773	1	Phosphoserine; by CDK2 By similarity
	Modified residue	1096	1	Phosphoserine; by CDK2 By similarity
	Modified residue	1223	1	N6-acetyllysine By similarity
	Modified residue	1264	1	Phosphothreonine; by CDK2 By similarity
	Modified residue	1343	1	Phosphothreonine; by CDK2 By similarity
Experimental info
	Sequence conflict	1141	1	S → P in BAC35255. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8BMA5 [UniParc]. Last modified February 5, 2008. Version 2. Checksum: 1035459489343314	FASTA	1,420	152,176
	10 20 30 40 50 60 MLLPSDVARL VLGYLQQENL TSTCQTFILE SSNLKEYAEH CTDEGFIPAC LLSLFGKNLT 70 80 90 100 110 120 TILNEYVAMK AKETSNDVPT IMSSLWKKLD HTLSQIRSMH SSPGFAAHQR ARTRNGIAEI 130 140 150 160 170 180 KRQRWLASQA APVSSELLVL PYASGQFTTS PLVATQAVKP TGPISTPVRS NIVVVNQSQP 190 200 210 220 230 240 QSTVTNTAGE SLNIIPGPQE RKTQTSLMSP GRRKSESQKK SLTSSGPHSS RNFQDPNAFA 250 260 270 280 290 300 VEKQMVIENA REKILSNKSL QEKLAENINK FLTSDSSVAQ VPKQTDSNPT EPETSIDELL 310 320 330 340 350 360 GLPSEIHMSE EAIQDILEQT ESDPAFQALF DLFDYGKTKN NKNMPQISSQ PMETNSNIVL 370 380 390 400 410 420 PEETNLTIKS SFETEESDGQ SGQPPFCTSY QNEDVLLNDL KSGNSHDVLP QESQENFSQI 430 440 450 460 470 480 SSNIQKKTFK TAIPAEQKCA LDITLESVSN LSDFNQRGSS AECNEHCSEL FASQIPTEAE 490 500 510 520 530 540 VAVGEKNSLS ADILSQSQYQ PDQPSVPVTS FVSLGGETND KNLVLSGKNS QLLSQSTPLT 550 560 570 580 590 600 TKPSKSQLCE NSNNIIKVKT NPQASESADS SETANRKTET NTVSPAAAQP QADCQDNSPL 610 620 630 640 650 660 QSKPPPGIGE SLGVNVTEKI EIHLEEPAPS DKQLSNDAAS VDLNPTESKT EPLQSASAQE 670 680 690 700 710 720 PEPPSVKDGD TIFLSLSEHN SCEEVALVLG EGNPVKNNNS LSSESGGSVG VSPETQNTDG 730 740 750 760 770 780 KTSNSTEVDA SSIVSLKIII SDDPFVSSDA ELNSAVSSIS GENLPTIILS SKSPAKNAEF 790 800 810 820 830 840 VTCLSSEETA SAVVSVEVGD SGSMEQNLLV LKPEEPMVNN TQNEDGIAFS ANVAPCVPKD 850 860 870 880 890 900 GGYIQLMPTT STAFGNSSNI LIATCMTDST ALGPTVSQSN VVVLPGSSAP MTAQPPQQQL 910 920 930 940 950 960 QTPPKSNSAF AVSQAVSPNF SQGSAIIIAS PVQPVLQGMV GMIPVSVVGQ NGNTFSTPPQ 970 980 990 1000 1010 1020 QVLHMPLAAP VCNRSIAQLP IPQKSQKAQG LRNKLITGKQ VNNLTNLSSL SEACHTQRTE 1030 1040 1050 1060 1070 1080 ASDKNIATEL GKKMEDTTIS LSGERVAPPS KPFESHRRVL CFDSTVSSVA NTQGSLYKMT 1090 1100 1110 1120 1130 1140 SENKEKKEAS FSHLDSPILS STLKPPPNNA IKREREKTVP KILSKSETAS SRHTTVKEVQ 1150 1160 1170 1180 1190 1200 SEKKVSPTEV ALESLHKATA NKENELCGDG ERPKNADTSK LPGGQQNGSL RNEKAIASLQ 1210 1220 1230 1240 1250 1260 ELTKKQATPS NNKNATSVGG TVKDQKQEQS KPASSLIGAE ILQDVPIHSP ANRSADTDLP 1270 1280 1290 1300 1310 1320 VPRTPGSGAG EKHKEEPSDS MKAPASRRCG EEGSMPRVMI PPVTADLPAC SPASETGSEN 1330 1340 1350 1360 1370 1380 SVSMAAHTLM ILSRAAIART TATPLKDNTQ QFRTSSRSTT KKRKIEELDE CERNSRTSGK 1390 1400 1410 1420 NLANSSVPMK KKKIKKKKLP SSFPAGMDVD KFLLSLHYDE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Cerebellum, Head and Muellerian duct.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"Proviral inactivation of the Npat gene of Mpv 20 mice results in early embryonic arrest." Di Fruscio M., Weiher H., Vanderhyden B.C., Imai T., Shiomi T., Hori T., Jaenisch R., Gray D.A. Mol. Cell. Biol. 17:4080-4086(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[4]	"NPAT expression is regulated by E2F and is essential for cell cycle progression." Gao G., Bracken A.P., Burkard K., Pasini D., Classon M., Attwooll C., Sagara M., Imai T., Helin K., Zhao J. Mol. Cell. Biol. 23:2821-2833(2003) [PubMed] [Europe PMC] [Abstract] Cited for: DEVELOPMENTAL STAGE.
[5]	"FLASH is required for histone transcription and S-phase progression." Barcaroli D., Bongiorno-Borbone L., Terrinoni A., Hofmann T.G., Rossi M., Knight R.A., Matera A.G., Melino G., De Laurenzi V. Proc. Natl. Acad. Sci. U.S.A. 103:14808-14812(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK033012 mRNA. Translation: BAC28125.1. AK042624 mRNA. Translation: BAC31308.1. AK053074 mRNA. Translation: BAC35255.1. AC156640 Genomic DNA. No translation available.
IPI	IPI00352320.
RefSeq	NP_001074621.1. NM_001081152.1.
UniGene	Mm.116713.
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q8BMA5.
Proteomic databases
PRIDE	Q8BMA5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000035850; ENSMUSP00000048709; ENSMUSG00000033054.
GeneID	244879.
KEGG	mmu:244879.
UCSC	uc009pmf.1. mouse.
Organism-specific databases
CTD	4863.
MGI	MGI:107605. Npat.
Phylogenomic databases
eggNOG	NOG29266.
GeneTree	ENSGT00390000012388.
HOGENOM	HOG000113840.
HOVERGEN	HBG108198.
InParanoid	Q8BMA5.
OMA	FDLFDYG.
OrthoDB	EOG4MW856.
Gene expression databases
Bgee	Q8BMA5.
CleanEx	MM_NPAT.
Genevestigator	Q8BMA5.
Family and domain databases
InterPro	IPR006594. LisH_dimerisation. [Graphical view]
SMART	SM00667. LisH. 1 hit. [Graphical view]
PROSITE	PS50896. LISH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	386460.
SOURCE	Search...

Entry information

Entry name

NPAT_MOUSE

Accession

Primary (citable) accession number: Q8BMA5
Secondary accession number(s): Q8BWA9, Q8BY06

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	February 5, 2008
Last modified:	March 6, 2013
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program