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Q8BMA5 (NPAT_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein NPAT
Gene names
Name:Npat
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1420 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Required for progression through the G1 and S phases of the cell cycle and for S phase entry. Activates transcription of the histone H2A, histone H2B, histone H3 and histone H4 genes in conjunction with MIZF. Also positively regulates the ATM, MIZF and PRKDC promoters. Transcriptional activation may be accomplished at least in part by the recruitment of the NuA4 histone acetyltransferase (HAT) complex to target gene promoters By similarity. Required for early embryonic development. Ref.3

Subunit structure

Interacts with the cylin/CDK complexes CCNE1/CDK2 and CCNA1/CDK2. Interacts with BZW1, CASP8AP2, CREBBP, MIZF and YY1. Interacts with the RUVBL1, RUVBL2 and TRRAP subunits of the NuA4 complex. May also interact with GAPDH, NME1, NME2 and STIP1 By similarity.

Subcellular location

Nucleus. Note: Concentrates in Cajal bodies tethered to histone gene clusters By similarity. Ref.5

Developmental stage

Expression peaks at the G1/S phase boundary. Ref.4

Domain

The LisH domain is required for the activation of histone gene transcription By similarity.

Post-translational modification

Phosphorylated at Ser-771, Ser-773, Ser-1096, Thr-1264 and Thr-1343 by CCNE1/CDK2 at G1-S transition and until prophase, which promotes association with histone gene clusters and stimulates activation of histone transcription. Also phosphorylated by CCNA1/CDK2 in vitro By similarity.

Sequence similarities

Belongs to the NPAT family.

Contains 1 LisH domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 14201420Protein NPAT
PRO_0000318164

Regions

Domain3 – 3533LisH
Region1 – 319319Interaction with MIZF By similarity
Region5 – 2521Required for activation of histone gene transcription and interaction with MIZF By similarity
Region121 – 14525Required for activation of histone gene transcription and interaction with MIZF By similarity
Region263 – 33977Mediates transcriptional activation By similarity
Region628 – 65225Required for acceleration of G1 phase By similarity
Region822 – 84726Required for acceleration of G1 phase By similarity
Region1033 – 104816Required for acceleration of G1 phase By similarity
Region1223 – 124725Required for acceleration of G1 phase By similarity
Region1319 – 134224Required for acceleration of G1 phase By similarity

Amino acid modifications

Modified residue7711Phosphoserine; by CDK2 By similarity
Modified residue7731Phosphoserine; by CDK2 By similarity
Modified residue10961Phosphoserine; by CDK2 By similarity
Modified residue12231N6-acetyllysine By similarity
Modified residue12641Phosphothreonine; by CDK2 By similarity
Modified residue13431Phosphothreonine; by CDK2 By similarity

Experimental info

Sequence conflict11411S → P in BAC35255. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q8BMA5 [UniParc].

Last modified February 5, 2008. Version 2.
Checksum: 1035459489343314

FASTA1,420152,176
        10         20         30         40         50         60 
MLLPSDVARL VLGYLQQENL TSTCQTFILE SSNLKEYAEH CTDEGFIPAC LLSLFGKNLT 

        70         80         90        100        110        120 
TILNEYVAMK AKETSNDVPT IMSSLWKKLD HTLSQIRSMH SSPGFAAHQR ARTRNGIAEI 

       130        140        150        160        170        180 
KRQRWLASQA APVSSELLVL PYASGQFTTS PLVATQAVKP TGPISTPVRS NIVVVNQSQP 

       190        200        210        220        230        240 
QSTVTNTAGE SLNIIPGPQE RKTQTSLMSP GRRKSESQKK SLTSSGPHSS RNFQDPNAFA 

       250        260        270        280        290        300 
VEKQMVIENA REKILSNKSL QEKLAENINK FLTSDSSVAQ VPKQTDSNPT EPETSIDELL 

       310        320        330        340        350        360 
GLPSEIHMSE EAIQDILEQT ESDPAFQALF DLFDYGKTKN NKNMPQISSQ PMETNSNIVL 

       370        380        390        400        410        420 
PEETNLTIKS SFETEESDGQ SGQPPFCTSY QNEDVLLNDL KSGNSHDVLP QESQENFSQI 

       430        440        450        460        470        480 
SSNIQKKTFK TAIPAEQKCA LDITLESVSN LSDFNQRGSS AECNEHCSEL FASQIPTEAE 

       490        500        510        520        530        540 
VAVGEKNSLS ADILSQSQYQ PDQPSVPVTS FVSLGGETND KNLVLSGKNS QLLSQSTPLT 

       550        560        570        580        590        600 
TKPSKSQLCE NSNNIIKVKT NPQASESADS SETANRKTET NTVSPAAAQP QADCQDNSPL 

       610        620        630        640        650        660 
QSKPPPGIGE SLGVNVTEKI EIHLEEPAPS DKQLSNDAAS VDLNPTESKT EPLQSASAQE 

       670        680        690        700        710        720 
PEPPSVKDGD TIFLSLSEHN SCEEVALVLG EGNPVKNNNS LSSESGGSVG VSPETQNTDG 

       730        740        750        760        770        780 
KTSNSTEVDA SSIVSLKIII SDDPFVSSDA ELNSAVSSIS GENLPTIILS SKSPAKNAEF 

       790        800        810        820        830        840 
VTCLSSEETA SAVVSVEVGD SGSMEQNLLV LKPEEPMVNN TQNEDGIAFS ANVAPCVPKD 

       850        860        870        880        890        900 
GGYIQLMPTT STAFGNSSNI LIATCMTDST ALGPTVSQSN VVVLPGSSAP MTAQPPQQQL 

       910        920        930        940        950        960 
QTPPKSNSAF AVSQAVSPNF SQGSAIIIAS PVQPVLQGMV GMIPVSVVGQ NGNTFSTPPQ 

       970        980        990       1000       1010       1020 
QVLHMPLAAP VCNRSIAQLP IPQKSQKAQG LRNKLITGKQ VNNLTNLSSL SEACHTQRTE 

      1030       1040       1050       1060       1070       1080 
ASDKNIATEL GKKMEDTTIS LSGERVAPPS KPFESHRRVL CFDSTVSSVA NTQGSLYKMT 

      1090       1100       1110       1120       1130       1140 
SENKEKKEAS FSHLDSPILS STLKPPPNNA IKREREKTVP KILSKSETAS SRHTTVKEVQ 

      1150       1160       1170       1180       1190       1200 
SEKKVSPTEV ALESLHKATA NKENELCGDG ERPKNADTSK LPGGQQNGSL RNEKAIASLQ 

      1210       1220       1230       1240       1250       1260 
ELTKKQATPS NNKNATSVGG TVKDQKQEQS KPASSLIGAE ILQDVPIHSP ANRSADTDLP 

      1270       1280       1290       1300       1310       1320 
VPRTPGSGAG EKHKEEPSDS MKAPASRRCG EEGSMPRVMI PPVTADLPAC SPASETGSEN 

      1330       1340       1350       1360       1370       1380 
SVSMAAHTLM ILSRAAIART TATPLKDNTQ QFRTSSRSTT KKRKIEELDE CERNSRTSGK 

      1390       1400       1410       1420 
NLANSSVPMK KKKIKKKKLP SSFPAGMDVD KFLLSLHYDE 

« Hide

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Cerebellum, Head and Muellerian duct.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"Proviral inactivation of the Npat gene of Mpv 20 mice results in early embryonic arrest."
Di Fruscio M., Weiher H., Vanderhyden B.C., Imai T., Shiomi T., Hori T., Jaenisch R., Gray D.A.
Mol. Cell. Biol. 17:4080-4086(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"NPAT expression is regulated by E2F and is essential for cell cycle progression."
Gao G., Bracken A.P., Burkard K., Pasini D., Classon M., Attwooll C., Sagara M., Imai T., Helin K., Zhao J.
Mol. Cell. Biol. 23:2821-2833(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
[5]"FLASH is required for histone transcription and S-phase progression."
Barcaroli D., Bongiorno-Borbone L., Terrinoni A., Hofmann T.G., Rossi M., Knight R.A., Matera A.G., Melino G., De Laurenzi V.
Proc. Natl. Acad. Sci. U.S.A. 103:14808-14812(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK033012 mRNA. Translation: BAC28125.1.
AK042624 mRNA. Translation: BAC31308.1.
AK053074 mRNA. Translation: BAC35255.1.
AC156640 Genomic DNA. No translation available.
RefSeqNP_001074621.1. NM_001081152.1.
UniGeneMm.116713.

3D structure databases

ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ8BMA5.

Proteomic databases

PRIDEQ8BMA5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000035850; ENSMUSP00000048709; ENSMUSG00000033054.
GeneID244879.
KEGGmmu:244879.
UCSCuc009pmf.1. mouse.

Organism-specific databases

CTD4863.
MGIMGI:107605. Npat.

Phylogenomic databases

eggNOGNOG29266.
GeneTreeENSGT00390000012388.
HOGENOMHOG000113840.
HOVERGENHBG108198.
InParanoidQ8BMA5.
OMAFDLFDYG.
OrthoDBEOG7JDQX0.
PhylomeDBQ8BMA5.
TreeFamTF332825.

Gene expression databases

BgeeQ8BMA5.
CleanExMM_NPAT.
GenevestigatorQ8BMA5.

Family and domain databases

InterProIPR006594. LisH_dimerisation.
[Graphical view]
SMARTSM00667. LisH. 1 hit.
[Graphical view]
PROSITEPS50896. LISH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio386460.
PROQ8BMA5.
SOURCESearch...

Entry information

Entry nameNPAT_MOUSE
AccessionPrimary (citable) accession number: Q8BMA5
Secondary accession number(s): Q8BWA9, Q8BY06
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 5, 2008
Last modified: April 16, 2014
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot