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Protein

Connector enhancer of kinase suppressor of ras 3

Gene

Cnksr3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probably involved in transepithelial sodium transport. Regulates aldosterone-induced and ENaC-mediated sodium transport possibly through regulation of the ERK pathway.1 Publication

GO - Biological processi

  • negative regulation of ERK1 and ERK2 cascade Source: UniProtKB
  • negative regulation of peptidyl-serine phosphorylation Source: UniProtKB
  • positive regulation of sodium ion transmembrane transporter activity Source: MGI
  • positive regulation of sodium ion transport Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Connector enhancer of kinase suppressor of ras 3
Short name:
Connector enhancer of KSR 3
Alternative name(s):
CNK homolog protein 3
Short name:
CNK3
CNKSR family member 3
Maguin-like protein
Gene namesi
Name:Cnksr3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:2674130. Cnksr3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 555555Connector enhancer of kinase suppressor of ras 3PRO_0000311106Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei381 – 3811PhosphoserineCombined sources
Modified residuei383 – 3831PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8BMA3.
PaxDbiQ8BMA3.
PRIDEiQ8BMA3.

PTM databases

iPTMnetiQ8BMA3.
PhosphoSiteiQ8BMA3.

Expressioni

Tissue specificityi

Expressed in kidney.1 Publication

Gene expression databases

BgeeiQ8BMA3.
CleanExiMM_CNKSR3.
GenevisibleiQ8BMA3. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000015346.

Structurei

3D structure databases

ProteinModelPortaliQ8BMA3.
SMRiQ8BMA3. Positions 2-76.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 7266SAMPROSITE-ProRule annotationAdd
BLAST
Domaini80 – 17495CRICPROSITE-ProRule annotationAdd
BLAST
Domaini211 – 29383PDZPROSITE-ProRule annotationAdd
BLAST
Domaini325 – 546222DUF1170Add
BLAST

Sequence similaritiesi

Belongs to the CNKSR family.Curated
Contains 1 CRIC domain.PROSITE-ProRule annotation
Contains 1 DUF1170 domain.Curated
Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation
Contains 1 SAM (sterile alpha motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1738. Eukaryota.
ENOG41104C7. LUCA.
GeneTreeiENSGT00390000017199.
HOGENOMiHOG000015752.
HOVERGENiHBG051040.
InParanoidiQ8BMA3.
OMAiVLPTKMR.
OrthoDBiEOG71ZP0W.
PhylomeDBiQ8BMA3.
TreeFamiTF326495.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
2.30.42.10. 1 hit.
InterProiIPR010599. CNKSR2.
IPR017874. CRIC_domain.
IPR001478. PDZ.
IPR001660. SAM.
IPR013761. SAM/pointed.
[Graphical view]
PfamiPF10534. CRIC_ras_sig. 1 hit.
PF06663. DUF1170. 1 hit.
PF00595. PDZ. 1 hit.
PF00536. SAM_1. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
SM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF50156. SSF50156. 1 hit.
PROSITEiPS51290. CRIC. 1 hit.
PS50106. PDZ. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BMA3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEPVTKWSPK QVVDWTRGLD DCLQPYVHKF EREKIDGEQL LKISHQDLEE
60 70 80 90 100
LGVTRIGHQE LVLEAVDLLC ALNYGLETDT MKNLVLKLRA SSHNLQNYIS
110 120 130 140 150
SRRKSPAYDG NTSRKPPNEF LTSVVELIGA AKALLAWLDR APFTGITDLS
160 170 180 190 200
VTKNKIIQLC LDLTTAVQKD CLIAEMEDKV LNVVKVLNGI CDKTMRSTTD
210 220 230 240 250
PVMSQCACLE EVHLPNVRPG EGLGMYIKST YDGLHVITGT TENSPADRSQ
260 270 280 290 300
KIHAGDEVIQ VNRQTVVGWQ LKNLVRKLRE NPTGVVLLLK KRPTGSFSFT
310 320 330 340 350
PAPLKNLRWK PPLVQTSPPP TTTQSPESTM DASLKKEKPA ILDLYIPPPP
360 370 380 390 400
AVPYSPRDEN VSFGYRGHSK SKQPLPVRKG SESPNSFLDQ ESQRRRFTIA
410 420 430 440 450
DSDQLPGYSV ETNVLPTKMR GKTPSYGKPR PLSMPADGNW MGIVDPFAKP
460 470 480 490 500
RGNGRKGEDA LCRYFSNERI TPITEESASP MYRFSRPLTE RHLVRGADYI
510 520 530 540 550
RGSRCYINSD LHSSATIPFQ EEGSKKKSAS SSAKASSGEP SLLVSWLTRL

KLLTH
Length:555
Mass (Da):61,870
Last modified:March 1, 2003 - v1
Checksum:i341AF9A4425904F2
GO
Isoform 2 (identifier: Q8BMA3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-241: Missing.
     242-243: EN → MQ

Show »
Length:314
Mass (Da):34,995
Checksum:i7A263D3AF0E51F79
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti243 – 2431N → Q in AAH31194 (PubMed:15489334).Curated
Sequence conflicti288 – 2881L → Q in BAE29189 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 241241Missing in isoform 2. 1 PublicationVSP_029391Add
BLAST
Alternative sequencei242 – 2432EN → MQ in isoform 2. 1 PublicationVSP_029615

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY151137 mRNA. Translation: AAN72835.1.
AK033015 mRNA. Translation: BAC28127.1.
AK149951 mRNA. Translation: BAE29189.1.
BC031194 mRNA. Translation: AAH31194.1.
CCDSiCCDS56690.1. [Q8BMA3-1]
RefSeqiNP_766134.1. NM_172546.2. [Q8BMA3-1]
UniGeneiMm.37984.

Genome annotation databases

EnsembliENSMUST00000015346; ENSMUSP00000015346; ENSMUSG00000015202. [Q8BMA3-1]
ENSMUST00000150282; ENSMUSP00000115863; ENSMUSG00000015202. [Q8BMA3-2]
GeneIDi215748.
KEGGimmu:215748.
UCSCiuc007efb.1. mouse. [Q8BMA3-1]
uc007efc.1. mouse. [Q8BMA3-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY151137 mRNA. Translation: AAN72835.1.
AK033015 mRNA. Translation: BAC28127.1.
AK149951 mRNA. Translation: BAE29189.1.
BC031194 mRNA. Translation: AAH31194.1.
CCDSiCCDS56690.1. [Q8BMA3-1]
RefSeqiNP_766134.1. NM_172546.2. [Q8BMA3-1]
UniGeneiMm.37984.

3D structure databases

ProteinModelPortaliQ8BMA3.
SMRiQ8BMA3. Positions 2-76.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000015346.

PTM databases

iPTMnetiQ8BMA3.
PhosphoSiteiQ8BMA3.

Proteomic databases

MaxQBiQ8BMA3.
PaxDbiQ8BMA3.
PRIDEiQ8BMA3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000015346; ENSMUSP00000015346; ENSMUSG00000015202. [Q8BMA3-1]
ENSMUST00000150282; ENSMUSP00000115863; ENSMUSG00000015202. [Q8BMA3-2]
GeneIDi215748.
KEGGimmu:215748.
UCSCiuc007efb.1. mouse. [Q8BMA3-1]
uc007efc.1. mouse. [Q8BMA3-2]

Organism-specific databases

CTDi154043.
MGIiMGI:2674130. Cnksr3.

Phylogenomic databases

eggNOGiKOG1738. Eukaryota.
ENOG41104C7. LUCA.
GeneTreeiENSGT00390000017199.
HOGENOMiHOG000015752.
HOVERGENiHBG051040.
InParanoidiQ8BMA3.
OMAiVLPTKMR.
OrthoDBiEOG71ZP0W.
PhylomeDBiQ8BMA3.
TreeFamiTF326495.

Miscellaneous databases

NextBioi374837.
PROiQ8BMA3.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BMA3.
CleanExiMM_CNKSR3.
GenevisibleiQ8BMA3. MM.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
2.30.42.10. 1 hit.
InterProiIPR010599. CNKSR2.
IPR017874. CRIC_domain.
IPR001478. PDZ.
IPR001660. SAM.
IPR013761. SAM/pointed.
[Graphical view]
PfamiPF10534. CRIC_ras_sig. 1 hit.
PF06663. DUF1170. 1 hit.
PF00595. PDZ. 1 hit.
PF00536. SAM_1. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
SM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF50156. SSF50156. 1 hit.
PROSITEiPS51290. CRIC. 1 hit.
PS50106. PDZ. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, expression and comparison of human and mouse ML (maguin-like) genes, and expression in mouse oogenesis."
    Tzolovsky G., McGurk L., Pathirana S., Slee R., Hillier S., Clinton M., Bownes M.
    Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: FVB/N.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Bone marrow and Muellerian duct.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: FVB/N-3.
    Tissue: Mammary tumor.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  5. "Cnksr3 is a direct mineralocorticoid receptor target gene and plays a key role in the regulation of the epithelial sodium channel."
    Ziera T., Irlbacher H., Fromm A., Latouche C., Krug S.M., Fromm M., Jaisser F., Borden S.A.
    FASEB J. 23:3936-3946(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-381 AND SER-383, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiCNKR3_MOUSE
AccessioniPrimary (citable) accession number: Q8BMA3
Secondary accession number(s): Q3UDS2, Q8K2K0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: March 1, 2003
Last modified: January 20, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.