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Protein

Connector enhancer of kinase suppressor of ras 3

Gene

Cnksr3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in transepithelial sodium transport. Regulates aldosterone-induced and epithelial sodium channel (ENaC)-mediated sodium transport through regulation of ENaC cell surface expression. Acts as a scaffold protein coordinating the assembly of an ENaC-regulatory complex (ERC).2 Publications

GO - Biological processi

  • negative regulation of ERK1 and ERK2 cascade Source: UniProtKB
  • negative regulation of peptidyl-serine phosphorylation Source: UniProtKB
  • positive regulation of sodium ion transmembrane transporter activity Source: MGI
  • positive regulation of sodium ion transport Source: UniProtKB

Names & Taxonomyi

Protein namesi
Recommended name:
Connector enhancer of kinase suppressor of ras 3
Short name:
Connector enhancer of KSR 3
Alternative name(s):
CNK homolog protein 3
Short name:
CNK3
CNKSR family member 3
Maguin-like protein
Gene namesi
Name:Cnksr3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:2674130 Cnksr3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003111061 – 555Connector enhancer of kinase suppressor of ras 3Add BLAST555

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei381PhosphoserineCombined sources1
Modified residuei383PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8BMA3
PeptideAtlasiQ8BMA3
PRIDEiQ8BMA3

PTM databases

iPTMnetiQ8BMA3
PhosphoSitePlusiQ8BMA3

Expressioni

Tissue specificityi

Expressed in kidney.1 Publication

Gene expression databases

BgeeiENSMUSG00000015202
CleanExiMM_CNKSR3
GenevisibleiQ8BMA3 MM

Interactioni

Subunit structurei

Interacts with epithelial sodium channel ENaC. Interacts directly with SCNN1A (ENaC subunit alpha) and SCNN1B (ENaC subunit beta) C-terminal tails. Interacts with ENaC regulatory proteins NEDD4L, RAF1 and SGK1.By similarity

Protein-protein interaction databases

BioGridi229657, 3 interactors
IntActiQ8BMA3, 3 interactors
STRINGi10090.ENSMUSP00000015346

Structurei

3D structure databases

ProteinModelPortaliQ8BMA3
SMRiQ8BMA3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 72SAMPROSITE-ProRule annotationAdd BLAST66
Domaini80 – 174CRICPROSITE-ProRule annotationAdd BLAST95
Domaini211 – 293PDZPROSITE-ProRule annotationAdd BLAST83
Domaini325 – 546DUF1170Add BLAST222

Domaini

The PDZ domain is required for interaction with ENaC and SGK1, but not for interaction with NEDDL4 and RAF1.By similarity

Sequence similaritiesi

Belongs to the CNKSR family.Curated

Phylogenomic databases

eggNOGiKOG1738 Eukaryota
ENOG41104C7 LUCA
GeneTreeiENSGT00390000017199
HOGENOMiHOG000015752
HOVERGENiHBG051040
InParanoidiQ8BMA3
OMAiKCKQPLP
OrthoDBiEOG091G037J
PhylomeDBiQ8BMA3
TreeFamiTF326495

Family and domain databases

InterProiView protein in InterPro
IPR010599 CNKSR2
IPR017874 CRIC_domain
IPR001478 PDZ
IPR036034 PDZ_sf
IPR001660 SAM
IPR013761 SAM/pointed_sf
PfamiView protein in Pfam
PF10534 CRIC_ras_sig, 1 hit
PF06663 DUF1170, 1 hit
PF00595 PDZ, 1 hit
PF00536 SAM_1, 1 hit
SMARTiView protein in SMART
SM00228 PDZ, 1 hit
SM00454 SAM, 1 hit
SUPFAMiSSF47769 SSF47769, 1 hit
SSF50156 SSF50156, 1 hit
PROSITEiView protein in PROSITE
PS51290 CRIC, 1 hit
PS50106 PDZ, 1 hit
PS50105 SAM_DOMAIN, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BMA3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEPVTKWSPK QVVDWTRGLD DCLQPYVHKF EREKIDGEQL LKISHQDLEE
60 70 80 90 100
LGVTRIGHQE LVLEAVDLLC ALNYGLETDT MKNLVLKLRA SSHNLQNYIS
110 120 130 140 150
SRRKSPAYDG NTSRKPPNEF LTSVVELIGA AKALLAWLDR APFTGITDLS
160 170 180 190 200
VTKNKIIQLC LDLTTAVQKD CLIAEMEDKV LNVVKVLNGI CDKTMRSTTD
210 220 230 240 250
PVMSQCACLE EVHLPNVRPG EGLGMYIKST YDGLHVITGT TENSPADRSQ
260 270 280 290 300
KIHAGDEVIQ VNRQTVVGWQ LKNLVRKLRE NPTGVVLLLK KRPTGSFSFT
310 320 330 340 350
PAPLKNLRWK PPLVQTSPPP TTTQSPESTM DASLKKEKPA ILDLYIPPPP
360 370 380 390 400
AVPYSPRDEN VSFGYRGHSK SKQPLPVRKG SESPNSFLDQ ESQRRRFTIA
410 420 430 440 450
DSDQLPGYSV ETNVLPTKMR GKTPSYGKPR PLSMPADGNW MGIVDPFAKP
460 470 480 490 500
RGNGRKGEDA LCRYFSNERI TPITEESASP MYRFSRPLTE RHLVRGADYI
510 520 530 540 550
RGSRCYINSD LHSSATIPFQ EEGSKKKSAS SSAKASSGEP SLLVSWLTRL

KLLTH
Length:555
Mass (Da):61,870
Last modified:March 1, 2003 - v1
Checksum:i341AF9A4425904F2
GO
Isoform 2 (identifier: Q8BMA3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-241: Missing.
     242-243: EN → MQ

Show »
Length:314
Mass (Da):34,995
Checksum:i7A263D3AF0E51F79
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti243N → Q in AAH31194 (PubMed:15489334).Curated1
Sequence conflicti288L → Q in BAE29189 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0293911 – 241Missing in isoform 2. 1 PublicationAdd BLAST241
Alternative sequenceiVSP_029615242 – 243EN → MQ in isoform 2. 1 Publication2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY151137 mRNA Translation: AAN72835.1
AK033015 mRNA Translation: BAC28127.1
AK149951 mRNA Translation: BAE29189.1
BC031194 mRNA Translation: AAH31194.1
CCDSiCCDS56690.1 [Q8BMA3-1]
RefSeqiNP_766134.1, NM_172546.2 [Q8BMA3-1]
UniGeneiMm.37984

Genome annotation databases

EnsembliENSMUST00000015346; ENSMUSP00000015346; ENSMUSG00000015202 [Q8BMA3-1]
ENSMUST00000150282; ENSMUSP00000115863; ENSMUSG00000015202 [Q8BMA3-2]
GeneIDi215748
KEGGimmu:215748
UCSCiuc007efb.1 mouse [Q8BMA3-1]
uc007efc.1 mouse [Q8BMA3-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCNKR3_MOUSE
AccessioniPrimary (citable) accession number: Q8BMA3
Secondary accession number(s): Q3UDS2, Q8K2K0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: March 1, 2003
Last modified: March 28, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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