UniProtKB - Q8BM65 (NYAP2_MOUSE)
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Protein
Neuronal tyrosine-phosphorylated phosphoinositide-3-kinase adapter 2
Gene
Nyap2
Organism
Mus musculus (Mouse)
Status
Functioni
Activates PI3K and concomitantly recruits the WAVE1 complex to the close vicinity of PI3K and regulates neuronal morphogenesis.1 Publication
GO - Biological processi
- neuron projection morphogenesis Source: UniProtKB
- phosphatidylinositol 3-kinase signaling Source: UniProtKB
Names & Taxonomyi
Protein namesi | Recommended name: Neuronal tyrosine-phosphorylated phosphoinositide-3-kinase adapter 2 |
Gene namesi | Name:Nyap2 Synonyms:Kiaa1486 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2443135. Nyap2. |
Subcellular locationi
Pathology & Biotechi
Disruption phenotypei
Triple knockout mice NYAP1/NYAP2/MYO16 are fertile and appear healthy. However, compared to wild-type mice they show a clear reduction in brain size, exhibiting a reduction in the size of the cortex and striatum, but not the olfactory bulb or corpus callosum. The total neurite length of neurons in these mice is also significantly shorter.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 277 | Y → F: Abolishes binding to PIK3R2. Slight reduction of phosphorylation in HEK293T cells. Abolishes phosphorylation in HEK293T cells and in neurons; when associated with F-300. 1 Publication | 1 | |
Mutagenesisi | 300 | Y → F: Reduced binding to PIK3R2. Slight reduction of phosphorylation in HEK293T cells. Abolishes phosphorylation in HEK293T cells and neurons; when associated with F-277. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000325831 | 1 – 682 | Neuronal tyrosine-phosphorylated phosphoinositide-3-kinase adapter 2Add BLAST | 682 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 81 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 120 | PhosphoserineCombined sources | 1 | |
Modified residuei | 238 | PhosphoserineCombined sources | 1 | |
Modified residuei | 300 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 463 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Phosphorylated on tyrosine residues by FYN upon stimulation with CNTN5. Phosphorylation begins at E16 and persists in adult brain.1 Publication
Keywords - PTMi
Methylation, PhosphoproteinProteomic databases
PaxDbi | Q8BM65. |
PRIDEi | Q8BM65. |
PTM databases
iPTMneti | Q8BM65. |
PhosphoSitePlusi | Q8BM65. |
Expressioni
Tissue specificityi
Expressed predominantly in brain where it is present in the neurons, but not in astrocytes or oligodendrites.1 Publication
Developmental stagei
Expression first detected in the lower neocortex at E18 and decreases thereafter. At postnatal day 1, also expressed in the striatum and the olfactory bulb.1 Publication
Gene expression databases
CleanExi | MM_9430031J16RIK. |
Interactioni
Subunit structurei
Interacts with ACOT9, ARHGAP26 and PIK3R2. Interacts with components of the WAVE1 complex, CYFIP1 and NCKAP1; this interaction mediates PI3K-WAVE1 association and actin cytoskeleton remodeling.1 Publication
Binary interactionsi
With | Entry | #Exp. | IntAct | Notes |
---|---|---|---|---|
PIK3R1 | P27986 | 3 | EBI-7447598,EBI-79464 | From Homo sapiens. |
Protein-protein interaction databases
BioGridi | 232283. 6 interactors. |
IntActi | Q8BM65. 11 interactors. |
MINTi | Q8BM65. |
STRINGi | 10090.ENSMUSP00000065468. |
Structurei
3D structure databases
ProteinModelPortali | Q8BM65. |
SMRi | Q8BM65. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 150 – 161 | Involved in CYFIP1- and NCKAP1-bindingAdd BLAST | 12 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 344 – 485 | Pro-richAdd BLAST | 142 | |
Compositional biasi | 413 – 600 | Ser-richAdd BLAST | 188 |
Sequence similaritiesi
Belongs to the NYAP family.Curated
Phylogenomic databases
eggNOGi | ENOG410IH9T. Eukaryota. ENOG410YEXE. LUCA. |
HOGENOMi | HOG000111596. |
HOVERGENi | HBG108036. |
InParanoidi | Q8BM65. |
PhylomeDBi | Q8BM65. |
Family and domain databases
InterProi | View protein in InterPro IPR026722. NYAP1/NYAP2. IPR026723. NYAP2. IPR029353. NYAP_C. |
PANTHERi | PTHR22633. PTHR22633. 1 hit. PTHR22633:SF1. PTHR22633:SF1. 1 hit. |
Pfami | View protein in Pfam PF15452. NYAP_C. 1 hit. |
s (5)i Sequence
Sequence statusi: Complete.
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q8BM65-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
10 20 30 40 50
MIPSKMMSAN PEEDPLDTFF QYIEDMGMKA YDGLVIQNAS DIARENDRLR
60 70 80 90 100
NETKLAYLKE KNEKRRRQEE TIKRIGGEVG RGQDASYAGK HFRMGFMTMP
110 120 130 140 150
APQDRLPHPC SSGFTVRSQS LHSVGGTEDD SSCGSRRQPP PKPKRDPSTK
160 170 180 190 200
LSTSSETVNS TAASKSGRSL ERAEGKFTVP ASHSPPRAST SGHLFPSPGS
210 220 230 240 250
QERNIKVSAK PRPHSDEYSK KIPPPKPKRN PNTQLSTSFD ETYIKKHVPR
260 270 280 290 300
RTSLPRDSSL SQVCSPAADP EEEEPVYIEM VGNILRDFRK EEDDQSEAVY
310 320 330 340 350
EEMKYPIFDD LGHDSKCDFD HHSCSSQCAT PTVPDLDFVK SSGPCTPKGL
360 370 380 390 400
LCDIPPPFPN LLSHRPPLLV FPPAPVHCSP NSDESPLTPL EVTKLPVLEN
410 420 430 440 450
VSYMKQPPGA CPSSLPSHGS SHAKDQTGAL GPAPGASILS SSPPPPSTLY
460 470 480 490 500
RTQSPHGYPK SHSTSPSPVS MGRSLTPLSL KRPPPYDAVH SGSLSRSSSS
510 520 530 540 550
VPHTTPRPVS QDGAKMVNAA VNTYSAAQSG SRSRTPTSPL EELTSLFTSG
560 570 580 590 600
RSLLRKSSSG RRSKEPAEKS TEELKVRSHS TEPLPKLDSK ERGHYGSSSS
610 620 630 640 650
REPVKAQEWD GTPGPPVVTS RMGRCSVSPT LLAGNHSSEP KVSCKLGRSA
660 670 680
STSGVPPPSV TPLRQASDLQ QSQVPSSLAN RD
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 54 | K → N in BAJ19139 (PubMed:21946561).Curated | 1 | |
Sequence conflicti | 54 | K → N in BAC33918 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 54 | K → N in BAE23236 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 54 | K → N in AAH82310 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_032436 | 175 – 206 | Missing in isoform 2 and isoform 5. 2 PublicationsAdd BLAST | 32 | |
Alternative sequenceiVSP_032437 | 639 – 682 | EPKVS…LANRD → GKHDPVLPNGSKSLEAAHMQ ISLRKPSPQLSLLPPRLAPQ THTLRSRHMKNATVPLFIAM ARETMLLEALLCSSLQLGKT LYQMSSKMTTGLNSSHCNL in isoform 3. 1 PublicationAdd BLAST | 44 | |
Alternative sequenceiVSP_032438 | 675 – 682 | PSSLANRD → ACMQWFHGDHTMLEMIEKKR CLCKEIKARQKTEKGLCKQD SMPILPSWKKNAGAKKYSPP PYSKQQTVFWDTAI in isoform 4 and isoform 5. 2 Publications | 8 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB429290 mRNA. Translation: BAJ19139.1. AK034747 mRNA. Translation: BAC28819.1. AK049778 mRNA. Translation: BAC33918.1. AK137094 mRNA. Translation: BAE23236.1. AC116105 Genomic DNA. No translation available. AC131336 Genomic DNA. No translation available. AC163450 Genomic DNA. No translation available. BC082310 mRNA. Translation: AAH82310.1. AK129372 mRNA. Translation: BAC98182.1. |
RefSeqi | NP_766437.2. NM_172849.3. XP_006496550.1. XM_006496487.3. XP_006496553.1. XM_006496490.3. |
UniGenei | Mm.313904. |
Genome annotation databases
GeneIDi | 241134. |
UCSCi | uc007brj.1. mouse. [Q8BM65-2] uc007brl.1. mouse. [Q8BM65-3] uc007brn.1. mouse. [Q8BM65-5] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Entry informationi
Entry namei | NYAP2_MOUSE | |
Accessioni | Q8BM65Primary (citable) accession number: Q8BM65 Secondary accession number(s): D3Z7K6 Q8BWV8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 18, 2008 |
Last sequence update: | March 1, 2003 | |
Last modified: | February 28, 2018 | |
This is version 89 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |