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Protein

Neuronal tyrosine-phosphorylated phosphoinositide-3-kinase adapter 2

Gene

Nyap2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Activates PI3K and concomitantly recruits the WAVE1 complex to the close vicinity of PI3K and regulates neuronal morphogenesis.1 Publication

GO - Biological processi

  • neuron projection morphogenesis Source: UniProtKB
  • phosphatidylinositol 3-kinase signaling Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal tyrosine-phosphorylated phosphoinositide-3-kinase adapter 2
Gene namesi
Name:Nyap2
Synonyms:Kiaa1486
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2443135. Nyap2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Triple knockout mice NYAP1/NYAP2/MYO16 are fertile and appear healthy. However, compared to wild-type mice they show a clear reduction in brain size, exhibiting a reduction in the size of the cortex and striatum, but not the olfactory bulb or corpus callosum. The total neurite length of neurons in these mice is also significantly shorter.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi277 – 2771Y → F: Abolishes binding to PIK3R2. Slight reduction of phosphorylation in HEK293T cells. Abolishes phosphorylation in HEK293T cells and in neurons; when associated with F-300. 1 Publication
Mutagenesisi300 – 3001Y → F: Reduced binding to PIK3R2. Slight reduction of phosphorylation in HEK293T cells. Abolishes phosphorylation in HEK293T cells and neurons; when associated with F-277. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 682682Neuronal tyrosine-phosphorylated phosphoinositide-3-kinase adapter 2PRO_0000325831Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei120 – 1201PhosphoserineCombined sources
Modified residuei238 – 2381PhosphoserineCombined sources
Modified residuei300 – 3001PhosphotyrosineCombined sources
Modified residuei463 – 4631PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated on tyrosine residues by FYN upon stimulation with CNTN5. Phosphorylation begins at E16 and persists in adult brain.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8BM65.
PaxDbiQ8BM65.
PRIDEiQ8BM65.

PTM databases

iPTMnetiQ8BM65.
PhosphoSiteiQ8BM65.

Expressioni

Tissue specificityi

Expressed predominantly in brain where it is present in the neurons, but not in astrocytes or oligodendrites.1 Publication

Developmental stagei

Expression first detected in the lower neocortex at E18 and decreases thereafter. At postnatal day 1, also expressed in the striatum and the olfactory bulb.1 Publication

Gene expression databases

BgeeiQ8BM65.
CleanExiMM_9430031J16RIK.

Interactioni

Subunit structurei

Interacts with ACOT9, ARHGAP26 and PIK3R2. Interacts with components of the WAVE1 complex, CYFIP1 and NCKAP1; this interaction mediates PI3K-WAVE1 association and actin cytoskeleton remodeling.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PIK3R1P279863EBI-7447598,EBI-79464From a different organism.

Protein-protein interaction databases

IntActiQ8BM65. 11 interactions.
MINTiMINT-8308425.
STRINGi10090.ENSMUSP00000065468.

Structurei

3D structure databases

ProteinModelPortaliQ8BM65.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni150 – 16112Involved in CYFIP1- and NCKAP1-bindingAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi344 – 485142Pro-richAdd
BLAST
Compositional biasi413 – 600188Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the NYAP family.Curated

Phylogenomic databases

eggNOGiENOG410IH9T. Eukaryota.
ENOG410YEXE. LUCA.
HOGENOMiHOG000111596.
HOVERGENiHBG108036.
InParanoidiQ8BM65.
OrthoDBiEOG7Z69C3.
PhylomeDBiQ8BM65.

Family and domain databases

InterProiIPR026722. NYAP1/NYAP2.
IPR026723. NYAP2.
IPR029353. NYAP_C.
[Graphical view]
PANTHERiPTHR22633. PTHR22633. 2 hits.
PTHR22633:SF1. PTHR22633:SF1. 2 hits.
PfamiPF15452. NYAP_C. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BM65-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIPSKMMSAN PEEDPLDTFF QYIEDMGMKA YDGLVIQNAS DIARENDRLR
60 70 80 90 100
NETKLAYLKE KNEKRRRQEE TIKRIGGEVG RGQDASYAGK HFRMGFMTMP
110 120 130 140 150
APQDRLPHPC SSGFTVRSQS LHSVGGTEDD SSCGSRRQPP PKPKRDPSTK
160 170 180 190 200
LSTSSETVNS TAASKSGRSL ERAEGKFTVP ASHSPPRAST SGHLFPSPGS
210 220 230 240 250
QERNIKVSAK PRPHSDEYSK KIPPPKPKRN PNTQLSTSFD ETYIKKHVPR
260 270 280 290 300
RTSLPRDSSL SQVCSPAADP EEEEPVYIEM VGNILRDFRK EEDDQSEAVY
310 320 330 340 350
EEMKYPIFDD LGHDSKCDFD HHSCSSQCAT PTVPDLDFVK SSGPCTPKGL
360 370 380 390 400
LCDIPPPFPN LLSHRPPLLV FPPAPVHCSP NSDESPLTPL EVTKLPVLEN
410 420 430 440 450
VSYMKQPPGA CPSSLPSHGS SHAKDQTGAL GPAPGASILS SSPPPPSTLY
460 470 480 490 500
RTQSPHGYPK SHSTSPSPVS MGRSLTPLSL KRPPPYDAVH SGSLSRSSSS
510 520 530 540 550
VPHTTPRPVS QDGAKMVNAA VNTYSAAQSG SRSRTPTSPL EELTSLFTSG
560 570 580 590 600
RSLLRKSSSG RRSKEPAEKS TEELKVRSHS TEPLPKLDSK ERGHYGSSSS
610 620 630 640 650
REPVKAQEWD GTPGPPVVTS RMGRCSVSPT LLAGNHSSEP KVSCKLGRSA
660 670 680
STSGVPPPSV TPLRQASDLQ QSQVPSSLAN RD
Length:682
Mass (Da):73,897
Last modified:March 1, 2003 - v1
Checksum:i76FDE6609FFDAC8F
GO
Isoform 2 (identifier: Q8BM65-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     175-206: Missing.

Show »
Length:650
Mass (Da):70,539
Checksum:iA4AA76776B304D1E
GO
Isoform 3 (identifier: Q8BM65-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     639-682: EPKVSCKLGR...QVPSSLANRD → GKHDPVLPNG...TGLNSSHCNL

Show »
Length:737
Mass (Da):80,193
Checksum:iD04540CF32970A15
GO
Isoform 4 (identifier: Q8BM65-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     675-682: PSSLANRD → ACMQWFHGDH...QQTVFWDTAI

Show »
Length:748
Mass (Da):81,683
Checksum:i4FB8D1013AB6977A
GO
Isoform 5 (identifier: Q8BM65-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     175-206: Missing.
     675-682: PSSLANRD → ACMQWFHGDH...QQTVFWDTAI

Show »
Length:716
Mass (Da):78,325
Checksum:iAE293039FC214184
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti54 – 541K → N in BAJ19139 (PubMed:21946561).Curated
Sequence conflicti54 – 541K → N in BAC33918 (PubMed:16141072).Curated
Sequence conflicti54 – 541K → N in BAE23236 (PubMed:16141072).Curated
Sequence conflicti54 – 541K → N in AAH82310 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei175 – 20632Missing in isoform 2 and isoform 5. 2 PublicationsVSP_032436Add
BLAST
Alternative sequencei639 – 68244EPKVS…LANRD → GKHDPVLPNGSKSLEAAHMQ ISLRKPSPQLSLLPPRLAPQ THTLRSRHMKNATVPLFIAM ARETMLLEALLCSSLQLGKT LYQMSSKMTTGLNSSHCNL in isoform 3. 1 PublicationVSP_032437Add
BLAST
Alternative sequencei675 – 6828PSSLANRD → ACMQWFHGDHTMLEMIEKKR CLCKEIKARQKTEKGLCKQD SMPILPSWKKNAGAKKYSPP PYSKQQTVFWDTAI in isoform 4 and isoform 5. 2 PublicationsVSP_032438

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB429290 mRNA. Translation: BAJ19139.1.
AK034747 mRNA. Translation: BAC28819.1.
AK049778 mRNA. Translation: BAC33918.1.
AK137094 mRNA. Translation: BAE23236.1.
AC116105 Genomic DNA. No translation available.
AC131336 Genomic DNA. No translation available.
AC163450 Genomic DNA. No translation available.
BC082310 mRNA. Translation: AAH82310.1.
AK129372 mRNA. Translation: BAC98182.1.
CCDSiCCDS15095.1. [Q8BM65-3]
RefSeqiNP_766437.2. NM_172849.3.
XP_006496550.1. XM_006496487.2.
XP_006496553.1. XM_006496490.2.
XP_011236988.1. XM_011238686.1.
UniGeneiMm.313904.

Genome annotation databases

GeneIDi241134.
KEGGimmu:241134.
UCSCiuc007brj.1. mouse. [Q8BM65-2]
uc007brl.1. mouse. [Q8BM65-3]
uc007brn.1. mouse. [Q8BM65-5]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB429290 mRNA. Translation: BAJ19139.1.
AK034747 mRNA. Translation: BAC28819.1.
AK049778 mRNA. Translation: BAC33918.1.
AK137094 mRNA. Translation: BAE23236.1.
AC116105 Genomic DNA. No translation available.
AC131336 Genomic DNA. No translation available.
AC163450 Genomic DNA. No translation available.
BC082310 mRNA. Translation: AAH82310.1.
AK129372 mRNA. Translation: BAC98182.1.
CCDSiCCDS15095.1. [Q8BM65-3]
RefSeqiNP_766437.2. NM_172849.3.
XP_006496550.1. XM_006496487.2.
XP_006496553.1. XM_006496490.2.
XP_011236988.1. XM_011238686.1.
UniGeneiMm.313904.

3D structure databases

ProteinModelPortaliQ8BM65.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BM65. 11 interactions.
MINTiMINT-8308425.
STRINGi10090.ENSMUSP00000065468.

PTM databases

iPTMnetiQ8BM65.
PhosphoSiteiQ8BM65.

Proteomic databases

MaxQBiQ8BM65.
PaxDbiQ8BM65.
PRIDEiQ8BM65.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi241134.
KEGGimmu:241134.
UCSCiuc007brj.1. mouse. [Q8BM65-2]
uc007brl.1. mouse. [Q8BM65-3]
uc007brn.1. mouse. [Q8BM65-5]

Organism-specific databases

CTDi57624.
MGIiMGI:2443135. Nyap2.
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410IH9T. Eukaryota.
ENOG410YEXE. LUCA.
HOGENOMiHOG000111596.
HOVERGENiHBG108036.
InParanoidiQ8BM65.
OrthoDBiEOG7Z69C3.
PhylomeDBiQ8BM65.

Miscellaneous databases

PROiQ8BM65.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BM65.
CleanExiMM_9430031J16RIK.

Family and domain databases

InterProiIPR026722. NYAP1/NYAP2.
IPR026723. NYAP2.
IPR029353. NYAP_C.
[Graphical view]
PANTHERiPTHR22633. PTHR22633. 2 hits.
PTHR22633:SF1. PTHR22633:SF1. 2 hits.
PfamiPF15452. NYAP_C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "NYAP: a phosphoprotein family that links PI3K to WAVE1 signalling in neurons."
    Yokoyama K., Tezuka T., Kotani M., Nakazawa T., Hoshina N., Shimoda Y., Kakuta S., Sudo K., Watanabe K., Iwakura Y., Yamamoto T.
    EMBO J. 30:4739-4754(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5), FUNCTION, INTERACTION WITH ACOT9; ARHGAP26; CYFIP1; NCKAP1 AND PIK3R2, SUBUNIT, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE, PHOSPHORYLATION, MUTAGENESIS OF TYR-277 AND TYR-300.
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Embryonic breast and Spinal cord.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Strain: C57BL/6J.
    Tissue: Brain.
  5. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 257-682 (ISOFORM 4).
    Tissue: Brain.
  6. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
    Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
    J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-300, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-120 AND SER-238, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiNYAP2_MOUSE
AccessioniPrimary (citable) accession number: Q8BM65
Secondary accession number(s): D3Z7K6
, Q641L6, Q6ZPP8, Q8BWV8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 1, 2003
Last modified: July 6, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.