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Protein

Pre-mRNA-splicing factor 18

Gene

Prpf18

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Participates in the second step of pre-mRNA splicing.By similarity

GO - Biological processi

Keywordsi

Biological processmRNA processing, mRNA splicing

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor 18
Alternative name(s):
PRP18 homolog
Gene namesi
Name:Prpf18
Synonyms:Prp18
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1914479 Prpf18

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000585831 – 342Pre-mRNA-splicing factor 18Add BLAST342

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ8BM39
PaxDbiQ8BM39
PeptideAtlasiQ8BM39
PRIDEiQ8BM39

PTM databases

iPTMnetiQ8BM39
PhosphoSitePlusiQ8BM39

Expressioni

Gene expression databases

BgeeiENSMUSG00000039449
ExpressionAtlasiQ8BM39 baseline and differential
GenevisibleiQ8BM39 MM

Interactioni

Subunit structurei

Heterodimer with PPIH. Interacts with PRPF4 and with the spliceosome. Part of a complex containing U4/U6 snRNPs (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000041428

Structurei

3D structure databases

ProteinModelPortaliQ8BM39
SMRiQ8BM39
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PRP18 family.Curated

Phylogenomic databases

eggNOGiKOG2808 Eukaryota
ENOG410XR90 LUCA
GeneTreeiENSGT00390000015073
HOGENOMiHOG000242389
HOVERGENiHBG053696
InParanoidiQ8BM39
KOiK12817
OMAiDICKHLL
OrthoDBiEOG091G0ID9
PhylomeDBiQ8BM39
TreeFamiTF315049

Family and domain databases

Gene3Di1.10.720.150, 1 hit
InterProiView protein in InterPro
IPR004098 Prp18
IPR014906 PRP4-like
IPR036285 PRP4-like_sf
PfamiView protein in Pfam
PF02840 Prp18, 1 hit
PF08799 PRP4, 1 hit
SMARTiView protein in SMART
SM00500 SFM, 1 hit
SUPFAMiSSF158230 SSF158230, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BM39-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDILKSEILR KRQLVEDRNL LVENKKYFKR SELARKEEEA YYERCGYKIQ
60 70 80 90 100
PKEDDQKPLT SSNPVLELEL AEEKLPMTLS RQEVIRRLRE RGEPIRLFGE
110 120 130 140 150
TDYDAFQRLR KIEILTPEVN KGLRNDLKAA LDKIDQQYLN EIVGGQEPGE
160 170 180 190 200
EDTQNDLKVH EENTTIEELE ALGESLGKGD DHKDMDIITK FLKFLLGVWA
210 220 230 240 250
KELNAREDYV KRSVQGKLNS ATQKQTESYL RPLFRKLRKR NLPADIKESI
260 270 280 290 300
TDIIKFMLQR EYVKANDAYL QMAIGNAPWP IGVTMVGIHA RTGREKIFSK
310 320 330 340
HVAHVLNDET QRKYIQGLKR LMTICQKHFP TDPSKCVEYN AL
Length:342
Mass (Da):39,890
Last modified:March 1, 2003 - v1
Checksum:iCF44DBB9D478ED99
GO
Isoform 2 (identifier: Q8BM39-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     171-175: ALGES → VVYPP
     176-342: Missing.

Note: No experimental confirmation available. May be due to an intron retention.
Show »
Length:175
Mass (Da):20,673
Checksum:i09A771D5E8D4FD98
GO

Sequence cautioni

The sequence BAC29527 differs from that shown. Intron retention.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti244A → V in BAC29527 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_008329171 – 175ALGES → VVYPP in isoform 2. 1 Publication5
Alternative sequenceiVSP_008330176 – 342Missing in isoform 2. 1 PublicationAdd BLAST167

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK035073 mRNA Translation: BAC28933.1
AK036668 mRNA Translation: BAC29527.1 Sequence problems.
AK135707 mRNA Translation: BAE22619.1
AK135890 mRNA Translation: BAE22717.1
AK139720 mRNA Translation: BAE24112.1
BC004573 mRNA Translation: AAH04573.1
BC058631 mRNA Translation: AAH58631.1
CCDSiCCDS15657.1 [Q8BM39-1]
RefSeqiNP_080321.2, NM_026045.3 [Q8BM39-1]
UniGeneiMm.38529

Genome annotation databases

EnsembliENSMUST00000035721; ENSMUSP00000041428; ENSMUSG00000039449 [Q8BM39-1]
ENSMUST00000152362; ENSMUSP00000116495; ENSMUSG00000039449 [Q8BM39-1]
GeneIDi67229
KEGGimmu:67229
UCSCiuc008iex.1 mouse [Q8BM39-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPRP18_MOUSE
AccessioniPrimary (citable) accession number: Q8BM39
Secondary accession number(s): Q3UX46, Q8BZ54
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: March 1, 2003
Last modified: March 28, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health