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Protein

Pre-mRNA-splicing factor 18

Gene

Prpf18

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Participates in the second step of pre-mRNA splicing.By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor 18
Alternative name(s):
PRP18 homolog
Gene namesi
Name:Prpf18
Synonyms:Prp18
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1914479. Prpf18.

Subcellular locationi

  • Nucleus speckle By similarity

  • Note: Colocalizes with spliceosomal snRNPs.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 342342Pre-mRNA-splicing factor 18PRO_0000058583Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ8BM39.
MaxQBiQ8BM39.
PaxDbiQ8BM39.
PeptideAtlasiQ8BM39.
PRIDEiQ8BM39.

PTM databases

iPTMnetiQ8BM39.
PhosphoSiteiQ8BM39.

Expressioni

Gene expression databases

BgeeiENSMUSG00000039449.
ExpressionAtlasiQ8BM39. baseline and differential.
GenevisibleiQ8BM39. MM.

Interactioni

Subunit structurei

Heterodimer with PPIH. Interacts with PRPF4 and with the spliceosome. Part of a complex containing U4/U6 snRNPs (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000041428.

Structurei

3D structure databases

ProteinModelPortaliQ8BM39.
SMRiQ8BM39. Positions 63-122.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PRP18 family.Curated

Phylogenomic databases

eggNOGiKOG2808. Eukaryota.
ENOG410XR90. LUCA.
GeneTreeiENSGT00390000015073.
HOGENOMiHOG000242389.
HOVERGENiHBG053696.
InParanoidiQ8BM39.
KOiK12817.
OMAiEANNAYM.
OrthoDBiEOG091G0ID9.
PhylomeDBiQ8BM39.
TreeFamiTF315049.

Family and domain databases

InterProiIPR004098. Prp18.
IPR014906. PRP4-like.
[Graphical view]
PfamiPF02840. Prp18. 1 hit.
PF08799. PRP4. 1 hit.
[Graphical view]
SMARTiSM00500. SFM. 1 hit.
[Graphical view]
SUPFAMiSSF158230. SSF158230. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BM39-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDILKSEILR KRQLVEDRNL LVENKKYFKR SELARKEEEA YYERCGYKIQ
60 70 80 90 100
PKEDDQKPLT SSNPVLELEL AEEKLPMTLS RQEVIRRLRE RGEPIRLFGE
110 120 130 140 150
TDYDAFQRLR KIEILTPEVN KGLRNDLKAA LDKIDQQYLN EIVGGQEPGE
160 170 180 190 200
EDTQNDLKVH EENTTIEELE ALGESLGKGD DHKDMDIITK FLKFLLGVWA
210 220 230 240 250
KELNAREDYV KRSVQGKLNS ATQKQTESYL RPLFRKLRKR NLPADIKESI
260 270 280 290 300
TDIIKFMLQR EYVKANDAYL QMAIGNAPWP IGVTMVGIHA RTGREKIFSK
310 320 330 340
HVAHVLNDET QRKYIQGLKR LMTICQKHFP TDPSKCVEYN AL
Length:342
Mass (Da):39,890
Last modified:March 1, 2003 - v1
Checksum:iCF44DBB9D478ED99
GO
Isoform 2 (identifier: Q8BM39-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     171-175: ALGES → VVYPP
     176-342: Missing.

Note: No experimental confirmation available. May be due to an intron retention.
Show »
Length:175
Mass (Da):20,673
Checksum:i09A771D5E8D4FD98
GO

Sequence cautioni

The sequence BAC29527 differs from that shown.Intron retention.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti244 – 2441A → V in BAC29527 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei171 – 1755ALGES → VVYPP in isoform 2. 1 PublicationVSP_008329
Alternative sequencei176 – 342167Missing in isoform 2. 1 PublicationVSP_008330Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK035073 mRNA. Translation: BAC28933.1.
AK036668 mRNA. Translation: BAC29527.1. Sequence problems.
AK135707 mRNA. Translation: BAE22619.1.
AK135890 mRNA. Translation: BAE22717.1.
AK139720 mRNA. Translation: BAE24112.1.
BC004573 mRNA. Translation: AAH04573.1.
BC058631 mRNA. Translation: AAH58631.1.
CCDSiCCDS15657.1. [Q8BM39-1]
RefSeqiNP_080321.2. NM_026045.3. [Q8BM39-1]
UniGeneiMm.38529.

Genome annotation databases

EnsembliENSMUST00000035721; ENSMUSP00000041428; ENSMUSG00000039449. [Q8BM39-1]
ENSMUST00000152362; ENSMUSP00000116495; ENSMUSG00000039449. [Q8BM39-1]
GeneIDi67229.
KEGGimmu:67229.
UCSCiuc008iex.1. mouse. [Q8BM39-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK035073 mRNA. Translation: BAC28933.1.
AK036668 mRNA. Translation: BAC29527.1. Sequence problems.
AK135707 mRNA. Translation: BAE22619.1.
AK135890 mRNA. Translation: BAE22717.1.
AK139720 mRNA. Translation: BAE24112.1.
BC004573 mRNA. Translation: AAH04573.1.
BC058631 mRNA. Translation: AAH58631.1.
CCDSiCCDS15657.1. [Q8BM39-1]
RefSeqiNP_080321.2. NM_026045.3. [Q8BM39-1]
UniGeneiMm.38529.

3D structure databases

ProteinModelPortaliQ8BM39.
SMRiQ8BM39. Positions 63-122.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000041428.

PTM databases

iPTMnetiQ8BM39.
PhosphoSiteiQ8BM39.

Proteomic databases

EPDiQ8BM39.
MaxQBiQ8BM39.
PaxDbiQ8BM39.
PeptideAtlasiQ8BM39.
PRIDEiQ8BM39.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035721; ENSMUSP00000041428; ENSMUSG00000039449. [Q8BM39-1]
ENSMUST00000152362; ENSMUSP00000116495; ENSMUSG00000039449. [Q8BM39-1]
GeneIDi67229.
KEGGimmu:67229.
UCSCiuc008iex.1. mouse. [Q8BM39-1]

Organism-specific databases

CTDi8559.
MGIiMGI:1914479. Prpf18.

Phylogenomic databases

eggNOGiKOG2808. Eukaryota.
ENOG410XR90. LUCA.
GeneTreeiENSGT00390000015073.
HOGENOMiHOG000242389.
HOVERGENiHBG053696.
InParanoidiQ8BM39.
KOiK12817.
OMAiEANNAYM.
OrthoDBiEOG091G0ID9.
PhylomeDBiQ8BM39.
TreeFamiTF315049.

Miscellaneous databases

ChiTaRSiPrpf18. mouse.
PROiQ8BM39.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000039449.
ExpressionAtlasiQ8BM39. baseline and differential.
GenevisibleiQ8BM39. MM.

Family and domain databases

InterProiIPR004098. Prp18.
IPR014906. PRP4-like.
[Graphical view]
PfamiPF02840. Prp18. 1 hit.
PF08799. PRP4. 1 hit.
[Graphical view]
SMARTiSM00500. SFM. 1 hit.
[Graphical view]
SUPFAMiSSF158230. SSF158230. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPRP18_MOUSE
AccessioniPrimary (citable) accession number: Q8BM39
Secondary accession number(s): Q3UX46, Q8BZ54
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: March 1, 2003
Last modified: September 7, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.