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Protein

Noelin-2

Gene

Olfm2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in transforming growth factor beta (TGF-beta)-induced smooth muscle differentiation (By similarity). TGF-beta induces expression and nuclear translocation of OLFM2 where it binds to SRF, causing its dissociation from the transcriptional repressor HEY2/HERP1 and facilitating binding of SRF to target genes (By similarity). Plays a role in AMPAR complex organization (PubMed:25218043). Is a regulator of vascular smooth-muscle cell (SMC) phenotypic switching, that acts by promoting RUNX2 and inhibiting MYOCD binding to SRF. SMC phenotypic switching is the process through which vascular SMCs undergo transition between a quiescent contractile phenotype and a proliferative synthetic phenotype in response to pathological stimuli. SMC phenotypic plasticity is essential for vascular development and remodeling (By similarity).By similarity1 Publication

GO - Biological processi

  • locomotory behavior Source: MGI
  • positive regulation of smooth muscle cell differentiation Source: UniProtKB
  • protein secretion Source: MGI
  • regulation of vascular smooth muscle cell dedifferentiation Source: UniProtKB
  • visual perception Source: MGI

Names & Taxonomyi

Protein namesi
Recommended name:
Noelin-2
Alternative name(s):
Olfactomedin-2
Gene namesi
Name:Olfm2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:3045350 Olfm2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cytoplasm, Membrane, Nucleus, Secreted, Synapse

Pathology & Biotechi

Disruption phenotypei

No gross abnormalities with normal lifespan but mutants display reduced exploration, locomotion and olfactory sensitivity, reduced amplitude of the first negative wave in the visual evoked potential test, abnormal motor coordination, anxiety-related behavior and changes in AMPAR complex composition.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 14By similarityAdd BLAST14
ChainiPRO_000002007915 – 448Noelin-2Add BLAST434

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi68N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi149N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi189 ↔ 371PROSITE-ProRule annotation
Glycosylationi269N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi304N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi393N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi435N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ8BM13
PaxDbiQ8BM13
PeptideAtlasiQ8BM13
PRIDEiQ8BM13

PTM databases

PhosphoSitePlusiQ8BM13

Expressioni

Tissue specificityi

Expressed in the brain (at protein level) (PubMed:22632720). In the developing eye, first detected at 12 dpc in the retinal pigmented epithelium and preferentially expressed in differentiating retinal ganglion cells between 15 and 18 dpc (PubMed:21228389). In the brain, expression is detected mainly in the olfactory bulb, cortex, piriform cortex, olfactory trabeculae, and inferior and superior colliculus (PubMed:25218043). In the adult eye, expression is detected mainly in retinal ganglion cells (PubMed:25218043). Expressed in carotid arteries (PubMed:28062493).4 Publications

Developmental stagei

During embryonic eye development, first detected at 12 dpc with maximum levels at 19.5 dpc and down-regulation of expression postnatally. In brain, levels increase from 13.5 dpc to postnatal day 6 and decrease in the adult.1 Publication

Gene expression databases

BgeeiENSMUSG00000032172
CleanExiMM_OLFM2
ExpressionAtlasiQ8BM13 baseline and differential
GenevisibleiQ8BM13 MM

Interactioni

Subunit structurei

Peripherally associated with AMPAR complex. AMPAR complex consists of an inner core made of 4 pore-forming GluA/GRIA proteins (GRIA1, GRIA2, GRIA3 and GRIA4) and 4 major auxiliary subunits arranged in a twofold symmetry. One of the two pairs of distinct binding sites is occupied either by CNIH2, CNIH3 or CACNG2, CACNG3. The other harbors CACNG2, CACNG3, CACNG4, CACNG8 or GSG1L. This inner core of AMPAR complex is complemented by outer core constituents binding directly to the GluA/GRIA proteins at sites distinct from the interaction sites of the inner core constituents. Outer core constituents include at least PRRT1, PRRT2, CKAMP44/SHISA9, FRRS1L and NRN1. The proteins of the inner and outer core serve as a platform for other, more peripherally associated AMPAR constituents, including OLFM2. Alone or in combination, these auxiliary subunits control the gating and pharmacology of the AMPAR complex and profoundly impact their biogenesis and protein processing (PubMed:22632720). Interacts with GRIA2 (PubMed:25218043). Interacts with OLFM1 and OLFM3 (By similarity). Interacts with SRF; the interaction promotes dissociation of SRF from the transcriptional repressor HEY2 (By similarity). Interacts with RUNX2 (By similarity).By similarity2 Publications

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034692

Structurei

3D structure databases

ProteinModelPortaliQ8BM13
SMRiQ8BM13
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini188 – 440Olfactomedin-likePROSITE-ProRule annotationAdd BLAST253

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili52 – 79Sequence analysisAdd BLAST28
Coiled coili130 – 187Sequence analysisAdd BLAST58

Keywords - Domaini

Coiled coil, Signal

Phylogenomic databases

eggNOGiENOG410INP8 Eukaryota
ENOG410ZRHT LUCA
GeneTreeiENSGT00760000119005
HOGENOMiHOG000232069
HOVERGENiHBG006513
InParanoidiQ8BM13
OMAiMWPLTVP
OrthoDBiEOG091G05HN
TreeFamiTF315964

Family and domain databases

InterProiView protein in InterPro
IPR031219 Noelin-2
IPR022082 Noelin_dom
IPR003112 Olfac-like_dom
PANTHERiPTHR23192:SF27 PTHR23192:SF27, 1 hit
PfamiView protein in Pfam
PF12308 Noelin-1, 1 hit
PF02191 OLF, 1 hit
SMARTiView protein in SMART
SM00284 OLF, 1 hit
PROSITEiView protein in PROSITE
PS51132 OLF, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8BM13-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKLRQTGTT IAGGQTLFQS PEEGWQLYTS AQAPDGKCVC TAVIPAQSTC
60 70 80 90 100
ARDGRSRELR QLMEKVQNVS QSMEVLELRT FRDLQYVRSM ETLMRSLDAR
110 120 130 140 150
LRAADGSVSA KSFQELKDRM TELLPLSSVL EQYKADTRTI VRLREEVRNL
160 170 180 190 200
SGNLAAIQEE MGAYGYEDLQ QRVMALEARL HACAQKLGCG KLTGVSNPIT
210 220 230 240 250
IRAMGSRFGS WMTDTMAPSA DSRVWYMDGY YKGRRVLEFR TLGDFIKGQN
260 270 280 290 300
FIQHLLPQPW AGTGHVVYNG SLFYNKYQSN VVVKYHFRSR SVLVQRSLPG
310 320 330 340 350
AGYNNTFPYS WGGFSDMDFM VDESGLWAVY TTNQNAGNIV VSRLDPHTLE
360 370 380 390 400
VVRSWDTGYP KRSAGEAFMI CGVLYVTNSH LAGAKVYFAY FTNTSSYEYT
410 420 430 440
DVPFHNQYSH ISMLDYNPRE RALYTWNNGH QVLYNVTLFH VISTAGDP
Length:448
Mass (Da):50,720
Last modified:July 27, 2011 - v2
Checksum:iC29ADDE209697A5F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti405H → N in BAC29750 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK037199 mRNA Translation: BAC29750.1
CH466522 Genomic DNA Translation: EDL25128.1
BC115981 mRNA Translation: AAI15982.1
BC117536 mRNA Translation: AAI17537.1
CCDSiCCDS40546.1
RefSeqiNP_776138.2, NM_173777.3
UniGeneiMm.293552

Genome annotation databases

EnsembliENSMUST00000034692; ENSMUSP00000034692; ENSMUSG00000032172
GeneIDi244723
KEGGimmu:244723
UCSCiuc009oje.1 mouse

Similar proteinsi

Entry informationi

Entry nameiNOE2_MOUSE
AccessioniPrimary (citable) accession number: Q8BM13
Secondary accession number(s): Q0VFZ0, Q0VG58
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: July 27, 2011
Last modified: March 28, 2018
This is version 103 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health