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Protein

Leucine-rich repeat and fibronectin type-III domain-containing protein 3

Gene

Lrfn3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cell adhesion molecule that mediates homophilic cell-cell adhesion in a Ca2+-independent manner. Promotes neurite outgrowth in hippocampal neurons.2 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

ReactomeiR-MMU-8849932. SALM protein interactions at the synapses.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine-rich repeat and fibronectin type-III domain-containing protein 3
Alternative name(s):
Synaptic adhesion-like molecule 4
Gene namesi
Name:Lrfn3
Synonyms:Salm4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2442512. Lrfn3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini17 – 539ExtracellularSequence analysisAdd BLAST523
Transmembranei540 – 560HelicalSequence analysisAdd BLAST21
Topological domaini561 – 626CytoplasmicSequence analysisAdd BLAST66

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16Sequence analysisAdd BLAST16
ChainiPRO_000001484217 – 626Leucine-rich repeat and fibronectin type-III domain-containing protein 3Add BLAST610

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi81N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi317 ↔ 366PROSITE-ProRule annotation
Glycosylationi339N-linked (GlcNAc...)Sequence analysis1
Glycosylationi348N-linked (GlcNAc...)Sequence analysis1
Glycosylationi393N-linked (GlcNAc...)Sequence analysis1
Glycosylationi462N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ8BLY3.
PaxDbiQ8BLY3.
PRIDEiQ8BLY3.

PTM databases

iPTMnetiQ8BLY3.
PhosphoSitePlusiQ8BLY3.

Expressioni

Tissue specificityi

Expressed in brain, testis, stomach, small intestine and kidney. Residually expressed in heart, lung, liver, skeletal muscle and uterus. In the brain, weak, but broad expression in the cerebral cortex and diencephalic nuclei. Also detected in other parts of the central nervous system, including the olfactory bulb, pons, cerebellum, and medulla oblongata, as well as in the peripheral nervous system, such as the ganglia of cranial nerves and the dorsal root ganglion during gestation.1 Publication

Developmental stagei

Expressed from 4.5 to 18.5 dpc. Initial levels are low until 6.5 dpc and residual from 7.5 to 9.5 dpc. Expression increases from 10.5 to 15.5 dpc before falling again to a level slightly higher than the one seen on 4.5 to 6.5 dpc. At 11.5 dpc, broadly expressed in the telencephalic and diencephalic vesicles. At 17.5 dpc, expressed in cerebral cortex, hippocampus, dorsal thalamus and amygdala.1 Publication

Gene expression databases

BgeeiENSMUSG00000036957.
GenevisibleiQ8BLY3. MM.

Interactioni

Subunit structurei

Can form heteromeric complexes with LRFN1, LRFN2, LRFN4 and LRFN5. Able to form homomeric complexes across cell junctions, between adjacent cells. Does not interact with DLG4.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000037616.

Structurei

3D structure databases

ProteinModelPortaliQ8BLY3.
SMRiQ8BLY3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 59LRRNTAdd BLAST41
Repeati84 – 105LRR 1Add BLAST22
Repeati108 – 129LRR 2Add BLAST22
Repeati132 – 153LRR 3Add BLAST22
Repeati157 – 178LRR 4Add BLAST22
Repeati181 – 202LRR 5Add BLAST22
Repeati205 – 226LRR 6Add BLAST22
Domaini249 – 295LRRCTAdd BLAST47
Domaini295 – 382Ig-likeAdd BLAST88
Domaini425 – 523Fibronectin type-IIIPROSITE-ProRule annotationAdd BLAST99

Domaini

Lacks a cytoplasmic PDZ-binding domain, which has been implicated in function of related Lrfn proteins.

Sequence similaritiesi

Belongs to the LRFN family.Curated
Contains 1 fibronectin type-III domain.PROSITE-ProRule annotation
Contains 6 LRR (leucine-rich) repeats.Curated
Contains 1 LRRCT domain.Curated
Contains 1 LRRNT domain.Curated

Keywords - Domaini

Immunoglobulin domain, Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0619. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00760000118831.
HOGENOMiHOG000237343.
HOVERGENiHBG052352.
InParanoidiQ8BLY3.
KOiK16356.
OMAiRYFWAVG.
OrthoDBiEOG091G04DL.
PhylomeDBiQ8BLY3.
TreeFamiTF350185.

Family and domain databases

CDDicd00063. FN3. 1 hit.
Gene3Di2.60.40.10. 2 hits.
3.80.10.10. 1 hit.
InterProiIPR000483. Cys-rich_flank_reg_C.
IPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
[Graphical view]
PfamiPF00041. fn3. 1 hit.
PF07679. I-set. 1 hit.
PF13855. LRR_8. 2 hits.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
SM00408. IGc2. 1 hit.
SM00369. LRR_TYP. 6 hits.
SM00082. LRRCT. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF49265. SSF49265. 1 hit.
SSF52058. SSF52058. 1 hit.
PROSITEiPS50853. FN3. 1 hit.
PS50835. IG_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8BLY3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVLPLLLCL LPLAPASSPP QPAISSPCPR RCRCQTQSMP LSVLCPGAGL
60 70 80 90 100
LFVPPSLDRR AAELRLADNF IAAVRRRDLA NMTGLLHLSL SRNTIRHVAA
110 120 130 140 150
GAFADLRALR ALHLDGNRLT SLGEGQLRGL VNLRHLILSN NQLAALAAGA
160 170 180 190 200
LDDCAETLED LDLSYNNLEQ LPWEALGRLG NVNTLGLDHN LLASVPAGAF
210 220 230 240 250
SRLHKLARLD MTSNRLTTIP PDPLFSRLPL LARPRGSPAS ALVLAFGGNP
260 270 280 290 300
LHCNCELVWL RRLAREDDLE ACASPPALGG RYFWAVGEEE FVCEPPVVTH
310 320 330 340 350
RSPPLAVPAG RPAALRCRAV GDPEPRVRWV SPQGRLLGNS SRARAFPNGT
360 370 380 390 400
LELLVTEPED GGTFTCIAAN AAGEATAAVE LTVGPPPPPQ LANSTSCDPP
410 420 430 440 450
RDGEPDALTP PSAASASAKV ADTVAPTDRG VQVTEHGATA ALVQWPDQRP
460 470 480 490 500
VPGIRMYQIQ YNSSADDILV YRMIPADSRS FLLTDLASGR TYDLCVLAVY
510 520 530 540 550
EDSATGLTAT RPVGCARFST EPALRPCAAP HAPFLGGTMI IALGGVIVAS
560 570 580 590 600
VLVFIFVLLL RYKVHGGQPP GKAKATAPVS SVCSQTNGAL GPVPSAPAPE
610 620
PAAPRAHTVV QLDCEPWGPS HEPAGP
Length:626
Mass (Da):66,056
Last modified:March 1, 2003 - v1
Checksum:i95321B8D058E0079
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ078787 mRNA. Translation: AAZ23616.1.
AK040916 mRNA. Translation: BAC30743.1.
CH466593 Genomic DNA. Translation: EDL24028.1.
BC066999 mRNA. Translation: AAH66999.1.
BC094593 mRNA. Translation: AAH94593.1.
CCDSiCCDS21088.1.
RefSeqiNP_780687.1. NM_175478.2.
UniGeneiMm.23157.

Genome annotation databases

EnsembliENSMUST00000046351; ENSMUSP00000037616; ENSMUSG00000036957.
GeneIDi233067.
KEGGimmu:233067.
UCSCiuc009gee.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ078787 mRNA. Translation: AAZ23616.1.
AK040916 mRNA. Translation: BAC30743.1.
CH466593 Genomic DNA. Translation: EDL24028.1.
BC066999 mRNA. Translation: AAH66999.1.
BC094593 mRNA. Translation: AAH94593.1.
CCDSiCCDS21088.1.
RefSeqiNP_780687.1. NM_175478.2.
UniGeneiMm.23157.

3D structure databases

ProteinModelPortaliQ8BLY3.
SMRiQ8BLY3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000037616.

PTM databases

iPTMnetiQ8BLY3.
PhosphoSitePlusiQ8BLY3.

Proteomic databases

MaxQBiQ8BLY3.
PaxDbiQ8BLY3.
PRIDEiQ8BLY3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000046351; ENSMUSP00000037616; ENSMUSG00000036957.
GeneIDi233067.
KEGGimmu:233067.
UCSCiuc009gee.1. mouse.

Organism-specific databases

CTDi79414.
MGIiMGI:2442512. Lrfn3.

Phylogenomic databases

eggNOGiKOG0619. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00760000118831.
HOGENOMiHOG000237343.
HOVERGENiHBG052352.
InParanoidiQ8BLY3.
KOiK16356.
OMAiRYFWAVG.
OrthoDBiEOG091G04DL.
PhylomeDBiQ8BLY3.
TreeFamiTF350185.

Enzyme and pathway databases

ReactomeiR-MMU-8849932. SALM protein interactions at the synapses.

Miscellaneous databases

PROiQ8BLY3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000036957.
GenevisibleiQ8BLY3. MM.

Family and domain databases

CDDicd00063. FN3. 1 hit.
Gene3Di2.60.40.10. 2 hits.
3.80.10.10. 1 hit.
InterProiIPR000483. Cys-rich_flank_reg_C.
IPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
[Graphical view]
PfamiPF00041. fn3. 1 hit.
PF07679. I-set. 1 hit.
PF13855. LRR_8. 2 hits.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
SM00408. IGc2. 1 hit.
SM00369. LRR_TYP. 6 hits.
SM00082. LRRCT. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF49265. SSF49265. 1 hit.
SSF52058. SSF52058. 1 hit.
PROSITEiPS50853. FN3. 1 hit.
PS50835. IG_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLRFN3_MOUSE
AccessioniPrimary (citable) accession number: Q8BLY3
Secondary accession number(s): Q505E2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 1, 2003
Last modified: November 30, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.