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Protein

SPARC-related modular calcium-binding protein 1

Gene

Smoc1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Probable regulator of osteoblast differentiation. Plays essential roles in both eye and limb development.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi382 – 3931Sequence analysisAdd BLAST12
Calcium bindingi419 – 4302Sequence analysisAdd BLAST12

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • extracellular matrix binding Source: MGI

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • extracellular matrix organization Source: MGI
  • eye development Source: UniProtKB
  • limb development Source: UniProtKB
  • positive regulation of cell-substrate adhesion Source: MGI
  • regulation of osteoblast differentiation Source: MGI
  • signal transduction Source: InterPro

Keywordsi

Molecular functionDevelopmental protein
Biological processDifferentiation
LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
SPARC-related modular calcium-binding protein 1
Alternative name(s):
SPARC-related gene protein
Secreted modular calcium-binding protein 1
Short name:
SMOC-1
Gene namesi
Name:Smoc1
Synonyms:Srg
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1929878. Smoc1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Basement membrane, Extracellular matrix, Secreted

Pathology & Biotechi

Disruption phenotypei

Null mice present with a phenotype resembling human microphthalmia with limb anomalies. The phenotype includes aplasia or hypoplasia of optic nerves, hypoplastic fibula and bowed tibia, and syndactyly in limbs. A thinned and irregular ganglion cell layer and atrophy of the anteroventral part of the retina is observed.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000002031726 – 463SPARC-related modular calcium-binding protein 1Add BLAST438

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi94 ↔ 117By similarity
Disulfide bondi128 ↔ 135By similarity
Disulfide bondi137 ↔ 157By similarity
Glycosylationi224N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi237 ↔ 261By similarity
Disulfide bondi272 ↔ 279By similarity
Disulfide bondi281 ↔ 302By similarity
Glycosylationi384N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8BLY1.
PRIDEiQ8BLY1.

PTM databases

iPTMnetiQ8BLY1.
PhosphoSitePlusiQ8BLY1.

Expressioni

Tissue specificityi

Widely expressed in many tissues with a strongest signal in ovary.

Developmental stagei

Found in the forebrain, midbrain, hindbrain, pharyngeal arch, somites, and forelimb buds at day E9.5. At day E10.5, strongly expressed in dorsal neural tube, developing pharyngeal arches and frontonasal region with low expression in the ectoderm overlying the developing optic vesicle, expression can be observed in the optic stalk. At E11.5 it is localized to the closure site of the optic cup. In developing limbs between days E10.5 and E11.5, it is found in both dorsal and ventral regions, but expression is predominant dorsal in hindlimb bud and not detected in the most anterior, posterior, and distal parts of limb buds. Expression coinciding with chondrogenic condensation can be observed at E12.5. Expression is restricted to future synovial joint regions at day E13.5.2 Publications

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000105976.

Structurei

3D structure databases

ProteinModelPortaliQ8BLY1.
SMRiQ8BLY1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini36 – 88Kazal-likePROSITE-ProRule annotationAdd BLAST53
Domaini91 – 157Thyroglobulin type-1 1PROSITE-ProRule annotationAdd BLAST67
Domaini234 – 302Thyroglobulin type-1 2PROSITE-ProRule annotationAdd BLAST69
Domaini369 – 404EF-hand 1Add BLAST36
Domaini406 – 441EF-hand 2Add BLAST36

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG4578. Eukaryota.
ENOG410YP7C. LUCA.
HOGENOMiHOG000234328.
HOVERGENiHBG058558.
InParanoidiQ8BLY1.
PhylomeDBiQ8BLY1.
TreeFamiTF320666.

Family and domain databases

Gene3Di4.10.800.10. 3 hits.
InterProiView protein in InterPro
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002350. Kazal_dom.
IPR036058. Kazal_dom_sf.
IPR019577. SPARC/Testican_Ca-bd-dom.
IPR000716. Thyroglobulin_1.
IPR036857. Thyroglobulin_1_sf.
PfamiView protein in Pfam
PF07648. Kazal_2. 1 hit.
PF10591. SPARC_Ca_bdg. 1 hit.
PF00086. Thyroglobulin_1. 2 hits.
SMARTiView protein in SMART
SM00280. KAZAL. 1 hit.
SM00211. TY. 2 hits.
SUPFAMiSSF100895. SSF100895. 1 hit.
SSF47473. SSF47473. 1 hit.
SSF57610. SSF57610. 2 hits.
PROSITEiView protein in PROSITE
PS00018. EF_HAND_1. 2 hits.
PS51465. KAZAL_2. 1 hit.
PS00484. THYROGLOBULIN_1_1. 2 hits.
PS51162. THYROGLOBULIN_1_2. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BLY1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLPARVRLLT PHLLLVLVQL SPAGGHRTTG PRFLISDRDP PCNPHCPRTQ
60 70 80 90 100
PKPICASDGR SYESMCEYQR AKCRDPALAV VHRGRCKDAG QSKCRLERAQ
110 120 130 140 150
ALEQAKKPQE AVFVPECGED GSFTQVQCHT YTGYCWCVTP DGKPISGSSV
160 170 180 190 200
QNKTPVCSGP VTDKPLSQGN SGRKVSFRFF LTLNSDDGSK PTPTMETQPV
210 220 230 240 250
FDGDEITAPT LWIKHLVIKD SKLNNTNVRN SEKVHSCDQE RQSALEEARQ
260 270 280 290 300
NPREGIVIPE CAPGGLYKPV QCHQSTGYCW CVLVDTGRPL PGTSTRYVMP
310 320 330 340 350
SCESDARAKS VEADDPFKDR ELPGCPEGKK MEFITSLLDA LTTDMVQAIN
360 370 380 390 400
SAAPTGGGRF SEPDPSHTLE ERVAHWYFSQ LDSNSSDDIN KREMKPFKRY
410 420 430 440 450
VKKKAKPKKC ARRFTDYCDL NKDKVISLPE LKGCLGVSKE GGSLGSFPQG
460
KRAGTNPFIG RLV
Note: No experimental confirmation available.
Length:463
Mass (Da):51,076
Last modified:October 31, 2003 - v2
Checksum:iC4EE880F34259698
GO
Isoform 2 (identifier: Q8BLY1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     175-185: Missing.

Show »
Length:452
Mass (Da):49,764
Checksum:iEAF73CB0AFC060F0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti311V → I in BAC30750 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_008721175 – 185Missing in isoform 2. 2 PublicationsAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF070470 mRNA. Translation: AAD41590.1.
AK040931 mRNA. Translation: BAC30750.1.
BC031804 mRNA. Translation: AAH31804.1.
CCDSiCCDS26017.1. [Q8BLY1-2]
CCDS49102.1. [Q8BLY1-1]
RefSeqiNP_001139689.1. NM_001146217.1.
NP_071711.2. NM_022316.2.
UniGeneiMm.273295.

Genome annotation databases

GeneIDi64075.
KEGGimmu:64075.
UCSCiuc007obu.2. mouse. [Q8BLY1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSMOC1_MOUSE
AccessioniPrimary (citable) accession number: Q8BLY1
Secondary accession number(s): Q9WVN9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: October 31, 2003
Last modified: October 25, 2017
This is version 130 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot