Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

SPARC-related modular calcium-binding protein 1

Gene

Smoc1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Probable regulator of osteoblast differentiation. Plays essential roles in both eye and limb development.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi382 – 3931Sequence analysisAdd BLAST12
Calcium bindingi419 – 4302Sequence analysisAdd BLAST12

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • extracellular matrix binding Source: MGI

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • extracellular matrix organization Source: MGI
  • eye development Source: UniProtKB
  • limb development Source: UniProtKB
  • positive regulation of cell-substrate adhesion Source: MGI
  • regulation of osteoblast differentiation Source: MGI
  • signal transduction Source: InterPro

Keywordsi

Molecular functionDevelopmental protein
Biological processDifferentiation
LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
SPARC-related modular calcium-binding protein 1
Alternative name(s):
SPARC-related gene protein
Secreted modular calcium-binding protein 1
Short name:
SMOC-1
Gene namesi
Name:Smoc1
Synonyms:Srg
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1929878 Smoc1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Basement membrane, Extracellular matrix, Secreted

Pathology & Biotechi

Disruption phenotypei

Null mice present with a phenotype resembling human microphthalmia with limb anomalies. The phenotype includes aplasia or hypoplasia of optic nerves, hypoplastic fibula and bowed tibia, and syndactyly in limbs. A thinned and irregular ganglion cell layer and atrophy of the anteroventral part of the retina is observed.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000002031726 – 463SPARC-related modular calcium-binding protein 1Add BLAST438

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi42 ↔ 73PROSITE-ProRule annotation
Disulfide bondi46 ↔ 66PROSITE-ProRule annotation
Disulfide bondi55 ↔ 86PROSITE-ProRule annotation
Disulfide bondi94 ↔ 117By similarity
Disulfide bondi128 ↔ 135By similarity
Disulfide bondi137 ↔ 157By similarity
Glycosylationi224N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi237 ↔ 261By similarity
Disulfide bondi272 ↔ 279By similarity
Disulfide bondi281 ↔ 302By similarity
Glycosylationi384N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8BLY1
PRIDEiQ8BLY1

PTM databases

iPTMnetiQ8BLY1
PhosphoSitePlusiQ8BLY1

Expressioni

Tissue specificityi

Widely expressed in many tissues with a strongest signal in ovary.

Developmental stagei

Found in the forebrain, midbrain, hindbrain, pharyngeal arch, somites, and forelimb buds at day E9.5. At day E10.5, strongly expressed in dorsal neural tube, developing pharyngeal arches and frontonasal region with low expression in the ectoderm overlying the developing optic vesicle, expression can be observed in the optic stalk. At E11.5 it is localized to the closure site of the optic cup. In developing limbs between days E10.5 and E11.5, it is found in both dorsal and ventral regions, but expression is predominant dorsal in hindlimb bud and not detected in the most anterior, posterior, and distal parts of limb buds. Expression coinciding with chondrogenic condensation can be observed at E12.5. Expression is restricted to future synovial joint regions at day E13.5.2 Publications

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000105976

Structurei

3D structure databases

ProteinModelPortaliQ8BLY1
SMRiQ8BLY1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini36 – 88Kazal-likePROSITE-ProRule annotationAdd BLAST53
Domaini91 – 157Thyroglobulin type-1 1PROSITE-ProRule annotationAdd BLAST67
Domaini234 – 302Thyroglobulin type-1 2PROSITE-ProRule annotationAdd BLAST69
Domaini369 – 404EF-hand 1Add BLAST36
Domaini406 – 441EF-hand 2Add BLAST36

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG4578 Eukaryota
ENOG410YP7C LUCA
HOGENOMiHOG000234328
HOVERGENiHBG058558
InParanoidiQ8BLY1
PhylomeDBiQ8BLY1
TreeFamiTF320666

Family and domain databases

CDDicd16240 EFh_SPARC_SMOC1, 1 hit
cd00191 TY, 2 hits
Gene3Di4.10.800.10, 2 hits
InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002350 Kazal_dom
IPR036058 Kazal_dom_sf
IPR037639 SMOC1_EC
IPR019577 SPARC/Testican_Ca-bd-dom
IPR000716 Thyroglobulin_1
IPR036857 Thyroglobulin_1_sf
PfamiView protein in Pfam
PF07648 Kazal_2, 1 hit
PF10591 SPARC_Ca_bdg, 1 hit
PF00086 Thyroglobulin_1, 2 hits
SMARTiView protein in SMART
SM00280 KAZAL, 1 hit
SM00211 TY, 2 hits
SUPFAMiSSF100895 SSF100895, 1 hit
SSF47473 SSF47473, 1 hit
SSF57610 SSF57610, 2 hits
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 2 hits
PS51465 KAZAL_2, 1 hit
PS00484 THYROGLOBULIN_1_1, 2 hits
PS51162 THYROGLOBULIN_1_2, 2 hits

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BLY1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLPARVRLLT PHLLLVLVQL SPAGGHRTTG PRFLISDRDP PCNPHCPRTQ
60 70 80 90 100
PKPICASDGR SYESMCEYQR AKCRDPALAV VHRGRCKDAG QSKCRLERAQ
110 120 130 140 150
ALEQAKKPQE AVFVPECGED GSFTQVQCHT YTGYCWCVTP DGKPISGSSV
160 170 180 190 200
QNKTPVCSGP VTDKPLSQGN SGRKVSFRFF LTLNSDDGSK PTPTMETQPV
210 220 230 240 250
FDGDEITAPT LWIKHLVIKD SKLNNTNVRN SEKVHSCDQE RQSALEEARQ
260 270 280 290 300
NPREGIVIPE CAPGGLYKPV QCHQSTGYCW CVLVDTGRPL PGTSTRYVMP
310 320 330 340 350
SCESDARAKS VEADDPFKDR ELPGCPEGKK MEFITSLLDA LTTDMVQAIN
360 370 380 390 400
SAAPTGGGRF SEPDPSHTLE ERVAHWYFSQ LDSNSSDDIN KREMKPFKRY
410 420 430 440 450
VKKKAKPKKC ARRFTDYCDL NKDKVISLPE LKGCLGVSKE GGSLGSFPQG
460
KRAGTNPFIG RLV
Note: No experimental confirmation available.
Length:463
Mass (Da):51,076
Last modified:October 31, 2003 - v2
Checksum:iC4EE880F34259698
GO
Isoform 2 (identifier: Q8BLY1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     175-185: Missing.

Show »
Length:452
Mass (Da):49,764
Checksum:iEAF73CB0AFC060F0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti311V → I in BAC30750 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_008721175 – 185Missing in isoform 2. 2 PublicationsAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF070470 mRNA Translation: AAD41590.1
AK040931 mRNA Translation: BAC30750.1
BC031804 mRNA Translation: AAH31804.1
CCDSiCCDS26017.1 [Q8BLY1-2]
CCDS49102.1 [Q8BLY1-1]
RefSeqiNP_001139689.1, NM_001146217.1
NP_071711.2, NM_022316.2
UniGeneiMm.273295

Genome annotation databases

GeneIDi64075
KEGGimmu:64075
UCSCiuc007obu.2 mouse [Q8BLY1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSMOC1_MOUSE
AccessioniPrimary (citable) accession number: Q8BLY1
Secondary accession number(s): Q9WVN9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: October 31, 2003
Last modified: May 23, 2018
This is version 133 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health