Q8BLX7 (COGA1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Collagen alpha-1(XVI) chain

Gene names

Name:

Col16a1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1580 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in mediating cell attachment and inducing integrin-mediated cellular reactions, such as cell spreading and alterations in cell morphology By similarity. UniProtKB Q07092
Subunit structure	Homotrimer. Interacts with FBN1, fibronectin and integrins ITGA1/ITGB1 and ITGA2/ITGB1. Integrin ITGA1/ITGB1 binds to a unique site within COL16A1 located close to its C-terminal end between collagenous domains COL1-COL3 By similarity. UniProtKB Q07092
Subcellular location	Secreted › extracellular space › extracellular matrix By similarity.
Tissue specificity	Expressed in most tissues examined with highest levels of expression observed in heart. Strongly expressed in cortical and medullar regions of kidney and more weakly expressed in lung. Also detected in the ciliary muscle of the eye, on the serosa layer lining the muscularis externa of intestinal tissue, and in the perimysium membrane lining both the cardiac muscle bundle and the smooth muscle tissue of the small intestine. Strongly stained in particulate or granular structures. Not detected in brain or skeletal muscle. Ref.3
Developmental stage	At embryonic day 8 (E8) of gestation no significant expression of mRNA or protein is observed, but strong signals are observed in placental trophoblasts. By E11 weak positive signals are observed in heart. During later stages of development, stronger expression is observed in a variety of tissues, particularly in the atrial and ventricular walls of the developing heart, spinal root neural fibers and skin. Ref.3
Domain	This sequence defines eighteen different domains, nine triple-helical domains (COL9 to COL1) and ten non-triple-helical domains (NC10 to NC1). The numerous interruptions in the triple helix may make this molecule either elastic or flexible.
Post-translational modification	Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Glycosylated By similarity. UniProtKB Q07092
Sequence similarities	Belongs to the fibril-associated collagens with interrupted helices (FACIT) family. Contains 9 collagen-like domains. Contains 1 laminin G-like domain.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Extracellular matrix Secreted
Coding sequence diversity	Alternative splicing
Domain	Collagen Repeat Signal
PTM	Glycoprotein Hydroxylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell adhesion Inferred from sequence or structural similarity. Source: UniProtKB cellular response to amino acid stimulus Inferred from direct assay PubMed 20548288. Source: MGI
Cellular_component	collagen Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	integrin binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.1 (identifier: Q8BLX7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform 2 Ref.1 (identifier: Q8BLX7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1430: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 1580	1559	Collagen alpha-1(XVI) chain		PRO_0000282960
Regions
	Domain	50 – 231	182	Laminin G-like
	Domain	375 – 424	50	Collagen-like 1
	Domain	590 – 643	54	Collagen-like 2
	Domain	676 – 725	50	Collagen-like 3
	Domain	797 – 848	52	Collagen-like 4
	Domain	1006 – 1063	58	Collagen-like 5
	Domain	1210 – 1263	54	Collagen-like 6
	Domain	1350 – 1407	58	Collagen-like 7
	Domain	1448 – 1500	53	Collagen-like 8
	Domain	1504 – 1552	49	Collagen-like 9
	Region	232 – 374	143	Nonhelical region 10 (NC10)
	Region	375 – 509	135	Triple-helical region 9 (COL9) with 3 imperfections
	Region	510 – 524	15	Nonhelical region 9 (NC9)
	Region	525 – 570	46	Triple-helical region 8 (COL8) with 1 imperfection
	Region	571 – 586	16	Nonhelical region 8 (NC8)
	Region	587 – 640	54	Triple-helical region 7 (COL7) with 1 imperfection
	Region	641 – 661	21	Nonhelical region 7 (NC7)
	Region	662 – 732	71	Triple-helical region 6 (COL6) with 1 imperfection
	Region	733 – 747	15	Nonhelical region 6 (NC6)
	Region	748 – 870	123	Triple-helical region 5 (COL5) with 3 imperfections
	Region	871 – 881	11	Nonhelical region 5 (NC5)
	Region	882 – 933	52	Triple-helical region 4 (COL4) with 2 imperfections
	Region	934 – 967	34	Nonhelical region 4 (NC4)
	Region	968 – 982	15	Triple-helical region 3 (COL3)
	Region	983 – 1005	23	Nonhelical region 3 (NC3)
	Region	1006 – 1409	404	Triple-helical region 2 (COL2) with 2 imperfections
	Region	1410 – 1448	39	Nonhelical region 2 (NC2)
	Region	1449 – 1554	106	Triple-helical region 1 (COL1) with 2 imperfections
	Region	1555 – 1580	26	Nonhelical region 1 (NC1)
	Motif	555 – 557	3	Cell attachment site Potential
	Motif	1000 – 1002	3	Cell attachment site Potential
	Motif	1206 – 1208	3	Cell attachment site Potential
Amino acid modifications
	Glycosylation	47	1	N-linked (GlcNAc...) Potential
	Glycosylation	327	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 1430	1430	Missing in isoform 2. Ref.1		VSP_052375
Experimental info
	Sequence conflict	726	1	K → R in BAC30765. Ref.1
	Sequence conflict	1119	1	R → Q in BAC30765. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 3, 2007. Version 2. Checksum: FC01635F6E410E3A	FASTA	1,580	155,805
	10 20 30 40 50 60 MLTSWAPGLW VLGLWATFSH GTNIGERCPT SQQEGLKLEH SSDPSTNVTG FNLIRRLNLM 70 80 90 100 110 120 KTSAIKKIRN PKGPLILRLG AAPVTQPTRR VFPRGLPEEF ALVLTVLLKK HTFRNTWYLF 130 140 150 160 170 180 QVTDANGYPQ ISLEVNSQER SLELRAQGQD GDFVSCIFPV PQLFDLRWHK LMLSVAGRVA 190 200 210 220 230 240 SVHVDCVSAS SQPLGPRQSI RPGGHVFLGL DAEQGKPVSF DLQQAHIYCD PELVLEEGCC 250 260 270 280 290 300 EILPGGCPPE TSKSRRDTQS NELIEINPQT EGKVYTRCFC LEEPQNSKVD AQLMGRNIQK 310 320 330 340 350 360 AERGTKVHQG TGVNECPPCA HSARESNVTL GPSGLKGGKG ERGLTGPSGP KGEKGARGND 370 380 390 400 410 420 CVRVSPDAPL QCVEGPKGEK GESGDLGPPG LPGPTGQKGQ KGEKGDGGLK GLPGKPGRDG 430 440 450 460 470 480 RPGEICVIGP KGQKGDPGFV GPEGLAGEPG PPGLPGPPGI GLPGTPGDPG GPPGPKGEKG 490 500 510 520 530 540 SSGIPGKEGP GGKPGKPGVP GTKGEKGDPC EVCPTLPEGS QNFVGLPGKP GPKGEPGDPA 550 560 570 580 590 600 PAWEGLGTVG LKGDRGDPGI QGMKGEKGEP CSSCSSGVGA QHLGPSPGHG LPGLPGTSGI 610 620 630 640 650 660 PGPRGLKGEK GSFGDTGPAG VPGSPGPVGP AGIKGAKGEP CEPCTALSEL QDGDMRVVHL 670 680 690 700 710 720 PGPAGEKGEP GSPGFGLPGK QGKAGERGLK GQKGDAGNPG DPGTPGITGQ PGISGEPGIR 730 740 750 760 770 780 GPAGPKGEKG DGCTACPSLQ GALTDVSGLP GKPGPKGEPG PEGVGHPGKP GQPGLPGVQG 790 800 810 820 830 840 PPGPKGTQGE PGPPGTGAEG PQGEPGTQGL PGTQGLPGPR GPPGSAGEKG AQGSPGPKGA 850 860 870 880 890 900 IGPMGPPGAG VSGPPGQKGS RGEKGEPGEC SCPSRGEPIF SGMPGAPGLW MGSSSQPGPQ 910 920 930 940 950 960 GPPGVPGPPG PPGMPGLQGV PGHNGLPGQP GLTAELGSLP IEKHLLKSIC GDCAQGQTAH 970 980 990 1000 1010 1020 PAFLLEKGEK GDQGIPGVPG FDNCARCFIE RERPRAEEAR GDNSEGEPGC SGSPGLPGPP 1030 1040 1050 1060 1070 1080 GMPGQRGEEG PPGMRGSPGP PGPIGLQGER GLTGLTGDKG EPGPPGQPGY PGAMGPPGLP 1090 1100 1110 1120 1130 1140 GIKGERGYTG PSGEKGESGP PGSEGLPGPQ GPAGPRGERG PQGSSGEKGD QGFQGQPGFP 1150 1160 1170 1180 1190 1200 GPPGPPGFPG KAGAPGPPGP QAEKGSEGIR GPSGLPGSPG PPGPPGIQGP AGLDGLDGKD 1210 1220 1230 1240 1250 1260 GKPGLRGDPG PAGPPGLMGP PGFKGKTGHP GLPGPKGDCG KPGPPGSSGR PGAEGEPGAM 1270 1280 1290 1300 1310 1320 GPQGRPGPPG HLGPPGQPGP PGLSTVGLKG DRGVPGERGL AGLPGQPGTP GHPGPPGEPG 1330 1340 1350 1360 1370 1380 SDGAAGKEGP PGKQGLYGPP GPKGDPGPAG QKGQAGEKGR SGMPGGPGKS GSMGPIGPPG 1390 1400 1410 1420 1430 1440 PAGERGHPGS PGPAGNPGLP GLPGSMGDMV NYDDIKRFIR QEIIKLFDER MAYYTSRMQF 1450 1460 1470 1480 1490 1500 PMEVAAAPGR PGPPGKDGAP GRPGAPGSPG LPGQIGREGR QGLPGMRGLP GTKGEKGDIG 1510 1520 1530 1540 1550 1560 VGIAGENGLP GPPGPQGPPG YGKMGATGPM GQQGIPGIPG PPGPMGQPGK AGHCNPSDCF 1570 1580 GAMPMEQQYP PMKSMKGPFG
	Isoform 2 [UniParc]. Checksum: D956EF9160987FC8	FASTA	150	14,823
	10 20 30 40 50 60 MAYYTSRMQF PMEVAAAPGR PGPPGKDGAP GRPGAPGSPG LPGQIGREGR QGLPGMRGLP 70 80 90 100 110 120 GTKGEKGDIG VGIAGENGLP GPPGPQGPPG YGKMGATGPM GQQGIPGIPG PPGPMGQPGK 130 140 150 AGHCNPSDCF GAMPMEQQYP PMKSMKGPFG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Embryo.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Tissue distribution and developmental expression of type XVI collagen in the mouse." Lai C.-H., Chu M.-L. Tissue Cell 28:155-164(1996) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK012212 mRNA. Translation: BAB28100.1. AK040971 mRNA. Translation: BAC30765.1. AL606925 Genomic DNA. Translation: CAM45907.1.
IPI	IPI00648306. IPI00649075.
RefSeq	NP_082542.3. NM_028266.5.
UniGene	Mm.41860.
3D structure databases
HSSP	HSSP built from PDB template 1Q7D based on UniProtKB Q15201.
ProteinModelPortal	Q8BLX7.
SMR	Q8BLX7. Positions 44-242.
ModBase	Search...
PTM databases
PhosphoSite	Q8BLX7.
Proteomic databases
PaxDb	Q8BLX7.
PRIDE	Q8BLX7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000044565; ENSMUSP00000035802; ENSMUSG00000040690.
GeneID	107581.
KEGG	mmu:107581.
UCSC	uc008uys.2. mouse. uc008uyv.2. mouse.
Organism-specific databases
CTD	1307.
MGI	MGI:1095396. Col16a1.
Phylogenomic databases
eggNOG	NOG12793.
GeneTree	ENSGT00700000104155.
HOGENOM	HOG000085653.
HOVERGEN	HBG071631.
InParanoid	Q8BLX7.
OMA	CEVCPTL.
OrthoDB	EOG47WNN3.
Gene expression databases
ArrayExpress	Q8BLX7.
Bgee	Q8BLX7.
CleanEx	MM_COL16A1.
Genevestigator	Q8BLX7.
Family and domain databases
InterPro	IPR008160. Collagen. IPR008985. ConA-like_lec_gl_sf. IPR001791. Laminin_G. [Graphical view]
Pfam	PF01391. Collagen. 9 hits. [Graphical view]
SMART	SM00210. TSPN. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS50025. LAM_G_DOMAIN. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	359082.
SOURCE	Search...

Entry information

Entry name

COGA1_MOUSE

Accession

Primary (citable) accession number: Q8BLX7
Secondary accession number(s): A3KFV5, Q9CZS2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	April 3, 2007
Last modified:	May 1, 2013
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program