Reviewed, UniProtKB/Swiss-Prot Q8BLR9 (HIF1N_MOUSE)

Names and origin

Protein names

Recommended name:
    Hypoxia-inducible factor 1-alpha inhibitor
    EC=1.14.11.16
Alternative name(s):
    Hypoxia-inducible factor asparagine hydroxylase

Gene names

Name:

Hif1an

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Hydroxylates HIF-1 alpha at 'Asp-799' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases By similarity.
Catalytic activity	Peptide L-aspartate + 2-oxoglutarate + O2 = peptide 3-hydroxy-L-aspartate + succinate + CO2.
Cofactor	Iron By similarity.
Subunit structure	Homodimer. Interacts with VHL and HIF1A By similarity.
Subcellular location	Nucleus Potential.
Sequence similarities	Contains 1 JmjC domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW peptide-aspartate beta-dioxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8BLR9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8BLR9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     241-263: QVDFDNPDYERFPNFRNVVGYET → GVKRRDQAKRETIAKEKYTKQNK
     264-349: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 349	349	Hypoxia-inducible factor 1-alpha inhibitor		PRO_0000083975
Regions
	Domain	142 – 307	166	JmjC
	Region	1 – 349	349	Interaction with VHL By similarity
	Region	126 – 349	224	Interaction with HIF1A By similarity
Sites
	Metal binding	199	1	Iron; catalytic By similarity
	Metal binding	201	1	Iron; catalytic By similarity
	Metal binding	279	1	Iron; catalytic By similarity
	Binding site	145	1	2-oxoglutarate By similarity
	Binding site	196	1	2-oxoglutarate By similarity
	Binding site	214	1	2-oxoglutarate By similarity
Natural variations
	Alternative sequence	241 – 263	23	QVDFD…VGYET → GVKRRDQAKRETIAKEKYTK QNK in isoform 2.		VSP_017537
	Alternative sequence	264 – 349	86	Missing in isoform 2.		VSP_017538

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 7, 2006. Version 2. Checksum: ED5C2B875EBCE76E	FASTA	349	40,241
	10 20 30 40 50 60 MAATAAEVAA SGSGEAREEA EAPGPAWDES QLRSYSFPTR PIPRLSQSDP RAEELIENEE 70 80 90 100 110 120 PVVLTDTNLV YPALKWDLEY LQENIGNGDF SVYSASTHKF LYYDEKKMGN FQNFKPRSNR 130 140 150 160 170 180 EEIKFHEFVE KLQAIQQRGG EERLYLQQTL NDTVGRKIVM DFLGFNWNWI NKQQGKRGWG 190 200 210 220 230 240 QLTSNLLLIG MEGNVTPAHY DEQQNFFAQI KGHKRCILFP PDQFECLYPY PVHHPCDRQS 250 260 270 280 290 300 QVDFDNPDYE RFPNFRNVVG YETVVGPGDV LYIPMYWWHH IESLLNGGIT ITVNFWYKGA 310 320 330 340 PTPKRIEYPL KAHQKVAIMR NIEKMLGEAL GNPQEVGPLL NTMIKGRYN
	Isoform 2. Checksum: F7386262A6C77954	FASTA	263	30,424
	10 20 30 40 50 60 MAATAAEVAA SGSGEAREEA EAPGPAWDES QLRSYSFPTR PIPRLSQSDP RAEELIENEE 70 80 90 100 110 120 PVVLTDTNLV YPALKWDLEY LQENIGNGDF SVYSASTHKF LYYDEKKMGN FQNFKPRSNR 130 140 150 160 170 180 EEIKFHEFVE KLQAIQQRGG EERLYLQQTL NDTVGRKIVM DFLGFNWNWI NKQQGKRGWG 190 200 210 220 230 240 QLTSNLLLIG MEGNVTPAHY DEQQNFFAQI KGHKRCILFP PDQFECLYPY PVHHPCDRQS 250 260 GVKRRDQAKR ETIAKEKYTK QNK

References

[1]

"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J and NOD. Tissue: Brain cortex.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK043636 mRNA. Translation: BAC31602.1. AK154774 mRNA. Translation: BAE32822.1.
IPI	IPI00652254. IPI00742317.
RefSeq	NP_795932.2.
UniGene	Mm.145278
3D structure databases
HSSP	HSSP built from PDB template 1MZE based on UniProtKB Q9NWT6.
SMR	Q8BLR9. Positions 15-349.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8BLR9.
Proteomic databases
PRIDE	Q8BLR9.
Genome annotation databases
Ensembl	ENSMUST00000040455; ENSMUSP00000035326; ENSMUSG00000036450; Mus musculus. [Genome view]
GeneID	319594.
KEGG	mmu:319594.
UCSC	uc008hpy.1. mouse.
Organism-specific databases
CTD	319594.
MGI	MGI:2442345. Hif1an.
Phylogenomic databases
HOGENOM	Q8BLR9.
HOVERGEN	Q8BLR9.
OMA	FAQIKGH.
Enzyme and pathway databases
BRENDA	1.14.11.16. 244.
Gene expression databases
ArrayExpress	Q8BLR9.
Bgee	Q8BLR9.
Genevestigator	Q8BLR9.
GermOnline	ENSMUSG00000036450. Mus musculus.
Family and domain databases
InterPro	IPR003347. TF_JmjC_AAH. [Graphical view]
SMART	SM00558. JmjC. 1 hit. [Graphical view]
PROSITE	PS51184. JMJC. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	395052.
SOURCE	Search...

Entry information

Entry name

HIF1N_MOUSE

Accession

Primary (citable) accession number: Q8BLR9
Secondary accession number(s): Q3U3G4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2003
Last sequence update:	March 7, 2006
Last modified:	November 3, 2009
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)