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Q8BLR9

- HIF1N_MOUSE

UniProt

Q8BLR9 - HIF1N_MOUSE

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Protein

Hypoxia-inducible factor 1-alpha inhibitor

Gene

Hif1an

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Hydroxylates HIF-1 alpha at 'Asp-799' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation (By similarity). Positively regulates ASB4 activity, promoting vascular differentiation.By similarity1 Publication

Catalytic activityi

Hypoxia-inducible factor-L-asparagine + 2-oxoglutarate + O2 = hypoxia-inducible factor-(3S)-3-hydroxy-L-asparagine + succinate + CO2.
Ankyrin-repeat-L-histidine + 2-oxoglutarate + O2 = ankyrin-repeat-(3S)-3-hydroxy-L-histidine + succinate + CO2.

Cofactori

Fe2+ ion.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei145 – 14512-oxoglutarateBy similarity
Binding sitei152 – 1521SubstrateBy similarity
Binding sitei196 – 19612-oxoglutarateBy similarity
Metal bindingi199 – 1991Iron; via tele nitrogen; catalyticBy similarity
Metal bindingi201 – 2011Iron; via tele nitrogen; catalyticBy similarity
Binding sitei205 – 20512-oxoglutarateBy similarity
Binding sitei214 – 21412-oxoglutarateBy similarity
Metal bindingi279 – 2791Iron; via tele nitrogen; catalyticBy similarity
Binding sitei294 – 29412-oxoglutarateBy similarity
Binding sitei300 – 3001Substrate; via amide nitrogenBy similarity
Binding sitei321 – 3211SubstrateBy similarity
Sitei340 – 3401Important for dimer formationBy similarity

GO - Molecular functioni

  1. ankyrin repeat binding Source: UniProtKB
  2. carboxylic acid binding Source: Ensembl
  3. cofactor binding Source: Ensembl
  4. iron ion binding Source: Ensembl
  5. Notch binding Source: UniProtKB
  6. peptidyl-asparagine 3-dioxygenase activity Source: UniProtKB
  7. peptidyl-histidine dioxygenase activity Source: UniProtKB
  8. zinc ion binding Source: Ensembl

GO - Biological processi

  1. negative regulation of Notch signaling pathway Source: UniProtKB
  2. negative regulation of transcription from RNA polymerase II promoter in response to hypoxia Source: UniProtKB
  3. oxidation-reduction process Source: UniProtKB
  4. peptidyl-asparagine hydroxylation Source: UniProtKB
  5. peptidyl-aspartic acid hydroxylation Source: UniProtKB
  6. peptidyl-histidine hydroxylation Source: UniProtKB
  7. positive regulation of myoblast differentiation Source: UniProtKB
  8. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_199104. Oxygen-dependent asparagine hydroxylation of Hypoxia-inducible Factor Alpha.

Names & Taxonomyi

Protein namesi
Recommended name:
Hypoxia-inducible factor 1-alpha inhibitor (EC:1.14.11.30, EC:1.14.11.n4)
Alternative name(s):
Hypoxia-inducible factor asparagine hydroxylase
Gene namesi
Name:Hif1an
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 19

Organism-specific databases

MGIiMGI:2442345. Hif1an.

Subcellular locationi

Nucleus By similarity. Cytoplasm By similarity. Cytoplasmperinuclear region By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
  2. perinuclear region of cytoplasm Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 349348Hypoxia-inducible factor 1-alpha inhibitorPRO_0000083975Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ8BLR9.
PaxDbiQ8BLR9.
PRIDEiQ8BLR9.

PTM databases

PhosphoSiteiQ8BLR9.

Expressioni

Gene expression databases

BgeeiQ8BLR9.
GenevestigatoriQ8BLR9.

Interactioni

Subunit structurei

Homodimer; homodimerization is essential for catalytic activity. Interacts with VHL and HIF1A. Part of a complex with VHL, HIF1A and HDAC1 or HDAC2 or HDAC3. Interacts with NFKB1 and NFKBIA. Interacts with NOTCH1, NOTCH2 and NOTCH3 but not with NOTCH4. Interacts with ABPA3. Interacts with TNKS2. Interacts with PPP1R12A (By similarity). Interacts with UBE3A (By similarity). Interacts with ASB4.By similarity1 Publication

Protein-protein interaction databases

BioGridi235386. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ8BLR9.
SMRiQ8BLR9. Positions 22-349.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini142 – 307166JmjCPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 125124Interaction with VHLBy similarityAdd
BLAST
Regioni181 – 1833Substrate bindingBy similarity
Regioni201 – 2033Substrate bindingBy similarity
Regioni238 – 2392Substrate bindingBy similarity

Sequence similaritiesi

Contains 1 JmjC domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG71927.
GeneTreeiENSGT00530000062914.
HOGENOMiHOG000008146.
HOVERGENiHBG051903.
InParanoidiQ8BLR9.
KOiK18055.
OMAiMIKGRYD.
OrthoDBiEOG72NRQ4.
PhylomeDBiQ8BLR9.
TreeFamiTF329609.

Family and domain databases

Gene3Di1.10.287.1010. 1 hit.
InterProiIPR027445. FIH-1.
IPR027452. FIH-1_domII.
IPR003347. JmjC_dom.
[Graphical view]
PANTHERiPTHR12461:SF19. PTHR12461:SF19. 1 hit.
SMARTiSM00558. JmjC. 1 hit.
[Graphical view]
PROSITEiPS51184. JMJC. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8BLR9-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAATAAEVAA SGSGEAREEA EAPGPAWDES QLRSYSFPTR PIPRLSQSDP
60 70 80 90 100
RAEELIENEE PVVLTDTNLV YPALKWDLEY LQENIGNGDF SVYSASTHKF
110 120 130 140 150
LYYDEKKMGN FQNFKPRSNR EEIKFHEFVE KLQAIQQRGG EERLYLQQTL
160 170 180 190 200
NDTVGRKIVM DFLGFNWNWI NKQQGKRGWG QLTSNLLLIG MEGNVTPAHY
210 220 230 240 250
DEQQNFFAQI KGHKRCILFP PDQFECLYPY PVHHPCDRQS QVDFDNPDYE
260 270 280 290 300
RFPNFRNVVG YETVVGPGDV LYIPMYWWHH IESLLNGGIT ITVNFWYKGA
310 320 330 340
PTPKRIEYPL KAHQKVAIMR NIEKMLGEAL GNPQEVGPLL NTMIKGRYN
Length:349
Mass (Da):40,241
Last modified:March 7, 2006 - v2
Checksum:iED5C2B875EBCE76E
GO
Isoform 2 (identifier: Q8BLR9-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     241-263: QVDFDNPDYERFPNFRNVVGYET → GVKRRDQAKRETIAKEKYTKQNK
     264-349: Missing.

Note: No experimental confirmation available.

Show »
Length:263
Mass (Da):30,424
Checksum:iF7386262A6C77954
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti177 – 1771R → L in AAI30014. (PubMed:15489334)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei241 – 26323QVDFD…VGYET → GVKRRDQAKRETIAKEKYTK QNK in isoform 2. 1 PublicationVSP_017537Add
BLAST
Alternative sequencei264 – 34986Missing in isoform 2. 1 PublicationVSP_017538Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK043636 mRNA. Translation: BAC31602.1.
AK154774 mRNA. Translation: BAE32822.1.
BC130013 mRNA. Translation: AAI30014.1.
CCDSiCCDS29852.1. [Q8BLR9-1]
RefSeqiNP_795932.2. NM_176958.3. [Q8BLR9-1]
UniGeneiMm.145278.

Genome annotation databases

EnsembliENSMUST00000040455; ENSMUSP00000035326; ENSMUSG00000036450. [Q8BLR9-1]
GeneIDi319594.
KEGGimmu:319594.
UCSCiuc008hpx.1. mouse. [Q8BLR9-2]
uc008hpy.1. mouse. [Q8BLR9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK043636 mRNA. Translation: BAC31602.1 .
AK154774 mRNA. Translation: BAE32822.1 .
BC130013 mRNA. Translation: AAI30014.1 .
CCDSi CCDS29852.1. [Q8BLR9-1 ]
RefSeqi NP_795932.2. NM_176958.3. [Q8BLR9-1 ]
UniGenei Mm.145278.

3D structure databases

ProteinModelPortali Q8BLR9.
SMRi Q8BLR9. Positions 22-349.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 235386. 2 interactions.

PTM databases

PhosphoSitei Q8BLR9.

Proteomic databases

MaxQBi Q8BLR9.
PaxDbi Q8BLR9.
PRIDEi Q8BLR9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000040455 ; ENSMUSP00000035326 ; ENSMUSG00000036450 . [Q8BLR9-1 ]
GeneIDi 319594.
KEGGi mmu:319594.
UCSCi uc008hpx.1. mouse. [Q8BLR9-2 ]
uc008hpy.1. mouse. [Q8BLR9-1 ]

Organism-specific databases

CTDi 55662.
MGIi MGI:2442345. Hif1an.

Phylogenomic databases

eggNOGi NOG71927.
GeneTreei ENSGT00530000062914.
HOGENOMi HOG000008146.
HOVERGENi HBG051903.
InParanoidi Q8BLR9.
KOi K18055.
OMAi MIKGRYD.
OrthoDBi EOG72NRQ4.
PhylomeDBi Q8BLR9.
TreeFami TF329609.

Enzyme and pathway databases

Reactomei REACT_199104. Oxygen-dependent asparagine hydroxylation of Hypoxia-inducible Factor Alpha.

Miscellaneous databases

NextBioi 395052.
PROi Q8BLR9.
SOURCEi Search...

Gene expression databases

Bgeei Q8BLR9.
Genevestigatori Q8BLR9.

Family and domain databases

Gene3Di 1.10.287.1010. 1 hit.
InterProi IPR027445. FIH-1.
IPR027452. FIH-1_domII.
IPR003347. JmjC_dom.
[Graphical view ]
PANTHERi PTHR12461:SF19. PTHR12461:SF19. 1 hit.
SMARTi SM00558. JmjC. 1 hit.
[Graphical view ]
PROSITEi PS51184. JMJC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J and NOD.
    Tissue: Brain cortex.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  3. "ASB4 is a hydroxylation substrate of FIH and promotes vascular differentiation via an oxygen-dependent mechanism."
    Ferguson J.E. III, Wu Y., Smith K., Charles P., Powers K., Wang H., Patterson C.
    Mol. Cell. Biol. 27:6407-6419(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ASB4.

Entry informationi

Entry nameiHIF1N_MOUSE
AccessioniPrimary (citable) accession number: Q8BLR9
Secondary accession number(s): A1L3B7, Q3U3G4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: March 7, 2006
Last modified: October 29, 2014
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3