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Protein

Krueppel-like factor 8

Gene

Klf8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Transcriptional repressor and activator. Binds to CACCC-boxes promoter elements. Also binds the GT-box of cyclin D1 promoter and mediates cell cycle progression at G1 phase as a downstream target of focal adhesion kinase (FAK) (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri270 – 29425C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri300 – 32425C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri330 – 35223C2H2-type 3PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: MGI
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Krueppel-like factor 8
Gene namesi
Name:Klf8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:2442430. Klf8.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 355355Krueppel-like factor 8PRO_0000294168Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki64 – 64Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity

Post-translational modificationi

Sumoylation at Lys-64 represses transcriptional activity and reduces cell cycle progression into the G1 phase. Has no effect on subcellular location.

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ8BLM0.
PRIDEiQ8BLM0.

PTM databases

iPTMnetiQ8BLM0.
PhosphoSiteiQ8BLM0.

Expressioni

Gene expression databases

BgeeiQ8BLM0.
CleanExiMM_KLF8.
ExpressionAtlasiQ8BLM0. baseline and differential.

Interactioni

Subunit structurei

Interacts with corepressor CtBP2. Interacts with PIAS1, PIAS2, AND PIAS4; the interaction with each ligase sumoylates KLF8 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000044317.

Structurei

3D structure databases

ProteinModelPortaliQ8BLM0.
SMRiQ8BLM0. Positions 11-47, 241-352.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 3 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri270 – 29425C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri300 – 32425C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri330 – 35223C2H2-type 3PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00760000118998.
HOGENOMiHOG000232138.
HOVERGENiHBG003941.
InParanoidiQ8BLM0.
KOiK09205.
OMAiSMLQAPV.
PhylomeDBiQ8BLM0.
TreeFamiTF350556.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BLM0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKFIDNMDV RIKSESGSMQ VFKQVTGPVP TRDPSARADR RNMTSPSFLA
60 70 80 90 100
ASPMENPALF NDIKIEPPEE LLESDFNMPQ VEPVDLSFHK PKAPLQPASM
110 120 130 140 150
LQAPIRPPKP PTAPQAIMVP TSADTVTSAA IPTVLTPGSI LASSQGTGGQ
160 170 180 190 200
PILHVIHTIP SVSLPNKMSG LKTIPLVVQS LPMVYTSLPT DGSPAAITVP
210 220 230 240 250
LIGGDGKSAG SVKVDPASMC PLEFPSDSDE SAIESGSSAL QSLQGFHHEP
260 270 280 290 300
ATMVHMQGEE SLDLKRRRIH QCDFAGCSKV YTKSSHLKAH RRIHTGEKPY
310 320 330 340 350
KCTWDGCSWK FARSDELTRH FRKHTGIKPF RCTDCNRSFS RSDHLSLHRR

RHDTM
Length:355
Mass (Da):38,845
Last modified:March 1, 2003 - v1
Checksum:i8DAA415948F6128F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK044185 mRNA. Translation: BAC31807.1.
BC070442 mRNA. Translation: AAH70442.1.
CCDSiCCDS30481.1.
RefSeqiNP_776141.1. NM_173780.4.
XP_006528916.1. XM_006528853.2.
XP_006528921.1. XM_006528858.2.
XP_006528922.1. XM_006528859.2.
XP_006528923.1. XM_006528860.2.
XP_011246123.1. XM_011247821.1.
XP_011246124.1. XM_011247822.1.
XP_011246125.1. XM_011247823.1.
XP_011246126.1. XM_011247824.1.
XP_011246127.1. XM_011247825.1.
UniGeneiMm.260751.
Mm.418152.

Genome annotation databases

EnsembliENSMUST00000039545; ENSMUSP00000044317; ENSMUSG00000041649.
ENSMUST00000112574; ENSMUSP00000108193; ENSMUSG00000041649.
GeneIDi245671.
KEGGimmu:245671.
UCSCiuc009uqu.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK044185 mRNA. Translation: BAC31807.1.
BC070442 mRNA. Translation: AAH70442.1.
CCDSiCCDS30481.1.
RefSeqiNP_776141.1. NM_173780.4.
XP_006528916.1. XM_006528853.2.
XP_006528921.1. XM_006528858.2.
XP_006528922.1. XM_006528859.2.
XP_006528923.1. XM_006528860.2.
XP_011246123.1. XM_011247821.1.
XP_011246124.1. XM_011247822.1.
XP_011246125.1. XM_011247823.1.
XP_011246126.1. XM_011247824.1.
XP_011246127.1. XM_011247825.1.
UniGeneiMm.260751.
Mm.418152.

3D structure databases

ProteinModelPortaliQ8BLM0.
SMRiQ8BLM0. Positions 11-47, 241-352.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000044317.

PTM databases

iPTMnetiQ8BLM0.
PhosphoSiteiQ8BLM0.

Proteomic databases

PaxDbiQ8BLM0.
PRIDEiQ8BLM0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000039545; ENSMUSP00000044317; ENSMUSG00000041649.
ENSMUST00000112574; ENSMUSP00000108193; ENSMUSG00000041649.
GeneIDi245671.
KEGGimmu:245671.
UCSCiuc009uqu.1. mouse.

Organism-specific databases

CTDi11279.
MGIiMGI:2442430. Klf8.

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00760000118998.
HOGENOMiHOG000232138.
HOVERGENiHBG003941.
InParanoidiQ8BLM0.
KOiK09205.
OMAiSMLQAPV.
PhylomeDBiQ8BLM0.
TreeFamiTF350556.

Miscellaneous databases

NextBioi386904.
PROiQ8BLM0.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BLM0.
CleanExiMM_KLF8.
ExpressionAtlasiQ8BLM0. baseline and differential.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain cortex.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.

Entry informationi

Entry nameiKLF8_MOUSE
AccessioniPrimary (citable) accession number: Q8BLM0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: March 1, 2003
Last modified: January 20, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.