Q8BLK9 (KS6C1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal protein S6 kinase delta-1
Short name=S6K-delta-1
EC=2.7.11.1

Alternative name(s):
52 kDa ribosomal protein S6 kinase

Gene names

Name:

Rps6kc1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1056 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in transmitting sphingosine-1 phosphate (SPP)-mediated signaling into the cell By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Interacts with SPHK1 and phosphatidylinositol 3-phosphate By similarity.
Subcellular location	Cytoplasm. Membrane. Note: Also found in some small dot-like or ring-shaped early endosome structures By similarity.
Domain	The first protein kinase domain appears to be a pseudokinase domain as it does not contain the classical characteristics, such as the ATP-binding motif, ATP-binding site and active site.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. S6 kinase subfamily. Contains 1 MIT domain. Contains 2 protein kinase domains. Contains 1 PX (phox homology) domain.
Sequence caution	The sequence BAC33510.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell communication Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphatidylinositol binding Inferred from electronic annotation. Source: InterPro protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8BLK9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform 2 (identifier: Q8BLK9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     36-47: Missing.
     89-158: Missing.
     961-961: E → G
     962-1056: Missing.

Note: Due to a partial intron retention. No experimental confirmation available.

Isoform 3 (identifier: Q8BLK9-3)

The sequence of this isoform differs from the canonical sequence as follows:
     317-347: Missing.
     932-947: HIQLTYFSRWSEVEDS → QELSQMHFFLFAVASN
     948-1056: Missing.

Note: Due to a partial intron retention. No experimental confirmation available.

Isoform 4 (identifier: Q8BLK9-4)

The sequence of this isoform differs from the canonical sequence as follows:
     279-336: ESSPTRREAV...WSLRSPAEEL → KVRQCHHWLE...IICRSVHSSK
     337-1056: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1056	1056	Ribosomal protein S6 kinase delta-1		PRO_0000233128
Regions
	Domain	8 – 132	125	PX
	Domain	276 – 304	29	MIT
	Domain	343 – 444	102	Protein kinase 1
	Domain	789 – 1046	258	Protein kinase 2
	Nucleotide binding	795 – 803	9	ATP By similarity
Sites
	Active site	919	1	Proton acceptor By similarity
	Binding site	823	1	ATP By similarity
Amino acid modifications
	Modified residue	422	1	Phosphoserine By similarity
	Modified residue	423	1	Phosphoserine Ref.4
	Modified residue	426	1	Phosphoserine By similarity
	Modified residue	577	1	Phosphoserine Ref.3
	Modified residue	634	1	Phosphoserine Ref.4
Natural variations
	Alternative sequence	36 – 47	12	Missing in isoform 2.		VSP_018043
	Alternative sequence	89 – 158	70	Missing in isoform 2.		VSP_018044
	Alternative sequence	279 – 336	58	ESSPT…PAEEL → KVRQCHHWLESLGSLQKSER NLEKPSHTRAQQDIASPLVT KITKSHHLIICRSVHSSK in isoform 4.		VSP_018045
	Alternative sequence	317 – 347	31	Missing in isoform 3.		VSP_018046
	Alternative sequence	337 – 1056	720	Missing in isoform 4.		VSP_018047
	Alternative sequence	932 – 947	16	HIQLT…EVEDS → QELSQMHFFLFAVASN in isoform 3.		VSP_018048
	Alternative sequence	948 – 1056	109	Missing in isoform 3.		VSP_018049
	Alternative sequence	961	1	E → G in isoform 2.		VSP_018050
	Alternative sequence	962 – 1056	95	Missing in isoform 2.		VSP_018051
Experimental info
	Sequence conflict	855 – 857	3	GSE → DSD in AAH58403. Ref.2
	Sequence conflict	855	1	G → D in BAC33510. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 2, 2006. Version 2. Checksum: 315E6BB9926E4F42	FASTA	1,056	115,712
	10 20 30 40 50 60 MTSPWGHSAD LARFYTVTEP QRHPRGYTVY KVTARVVSRR NPEDVQEIIV WRRYSDFKKL 70 80 90 100 110 120 HRELWQIHRN AFRHSELFPP FAKGTVFGRF DKTVIEERRQ CAEDLLQFSA NIPALYNSRQ 130 140 150 160 170 180 LQDFFKGGVI SDGSELIGPA EAYPDSPANA FPECGTEGFS SDSDLLSLTV DADSLAEVDD 190 200 210 220 230 240 GMASRQGSPS RTFGLSLSTD SSAVGAVASD SEPSRVEDRE SRSLFPSSLK PRLGRRDYLE 250 260 270 280 290 300 KAGELIKLAL KKEEEDDYEA ASDFYRKGVD LLLEGVQGES SPTRREAVKR RTAEYLMRAE 310 320 330 340 350 360 SICSLRAAPQ LHTGPQPPGS LSSRPPWSLR SPAEELKAFR VLGVIDKVLL VMDTRTEQTF 370 380 390 400 410 420 ILKGLRKSSE CSRNRKTIIP RCVPNMVCLH TYIISEESVF LVLQRAEGGK LWSYISKFLN 430 440 450 460 470 480 RSSQESLDIK EGRPSMPPRV CLQQPSASPQ GGSSFESRGS DTGSMLKALP LKTSLTPSSQ 490 500 510 520 530 540 DDSNQEDDGQ PSSPKWLDSG SSSEDECTAG YLTLCNEYGQ EKMDLVSLSE ESVMQPEGDK 550 560 570 580 590 600 ADTQAVSSPA SLATGSVSPS THLRVFSGGE DLEAVSSPPT SESLSRSKNS PMEFFRIDSK 610 620 630 640 650 660 DSTSELLGLD FGEKLHSLKP EPLKALFTLE DGDSPSQSLD PGESKRESEA QDSVSRGSDD 670 680 690 700 710 720 SVPVISFKEA AAEAISGAEE GRPDLLVNLP GELQPTKEAS AMDPKFSQAS AGRLDSKLLE 730 740 750 760 770 780 APDVLCLRLS SEQCHGLGPE GPEELSDPTE FCPGGVIPEH DAQADPGVLF EAAVDHRSSP 790 800 810 820 830 840 DQFLFSSLRS ESDRLGQVEV VVTAQALQES LFHISSPCSG ANKEHSAYAD TATSEEVLLF 850 860 870 880 890 900 TEPTKEEANS LFQRGSEAQE RGVGAGEADK EIHQIFEDLD KRLAASSRFF IPEGCIQRWA 910 920 930 940 950 960 AEMVVALDAL HREGIVCRDL NPNNILLNDG GHIQLTYFSR WSEVEDSCDS DAVARMYCAP 970 980 990 1000 1010 1020 EVGAVTEETE ACDWWSLGAV LFELLTGKTL VECHPAGINT HTTLNMPGCV SEEARSLIQQ 1030 1040 1050 LLQFNPMERL GAGVAGVEDI KSHPFFTPVD WAELTR
	Isoform 2 [UniParc]. Checksum: 6B5E259A161D99B8	FASTA	879	96,202
	10 20 30 40 50 60 MTSPWGHSAD LARFYTVTEP QRHPRGYTVY KVTARIIVWR RYSDFKKLHR ELWQIHRNAF 70 80 90 100 110 120 RHSELFPPFA KGTVFGFSSD SDLLSLTVDA DSLAEVDDGM ASRQGSPSRT FGLSLSTDSS 130 140 150 160 170 180 AVGAVASDSE PSRVEDRESR SLFPSSLKPR LGRRDYLEKA GELIKLALKK EEEDDYEAAS 190 200 210 220 230 240 DFYRKGVDLL LEGVQGESSP TRREAVKRRT AEYLMRAESI CSLRAAPQLH TGPQPPGSLS 250 260 270 280 290 300 SRPPWSLRSP AEELKAFRVL GVIDKVLLVM DTRTEQTFIL KGLRKSSECS RNRKTIIPRC 310 320 330 340 350 360 VPNMVCLHTY IISEESVFLV LQRAEGGKLW SYISKFLNRS SQESLDIKEG RPSMPPRVCL 370 380 390 400 410 420 QQPSASPQGG SSFESRGSDT GSMLKALPLK TSLTPSSQDD SNQEDDGQPS SPKWLDSGSS 430 440 450 460 470 480 SEDECTAGYL TLCNEYGQEK MDLVSLSEES VMQPEGDKAD TQAVSSPASL ATGSVSPSTH 490 500 510 520 530 540 LRVFSGGEDL EAVSSPPTSE SLSRSKNSPM EFFRIDSKDS TSELLGLDFG EKLHSLKPEP 550 560 570 580 590 600 LKALFTLEDG DSPSQSLDPG ESKRESEAQD SVSRGSDDSV PVISFKEAAA EAISGAEEGR 610 620 630 640 650 660 PDLLVNLPGE LQPTKEASAM DPKFSQASAG RLDSKLLEAP DVLCLRLSSE QCHGLGPEGP 670 680 690 700 710 720 EELSDPTEFC PGGVIPEHDA QADPGVLFEA AVDHRSSPDQ FLFSSLRSES DRLGQVEVVV 730 740 750 760 770 780 TAQALQESLF HISSPCSGAN KEHSAYADTA TSEEVLLFTE PTKEEANSLF QRGSEAQERG 790 800 810 820 830 840 VGAGEADKEI HQIFEDLDKR LAASSRFFIP EGCIQRWAAE MVVALDALHR EGIVCRDLNP 850 860 870 NNILLNDGGH IQLTYFSRWS EVEDSCDSDA VARMYCAPG
	Isoform 3 [UniParc]. Checksum: 329F9AC0A327F228	FASTA	916	100,333
	10 20 30 40 50 60 MTSPWGHSAD LARFYTVTEP QRHPRGYTVY KVTARVVSRR NPEDVQEIIV WRRYSDFKKL 70 80 90 100 110 120 HRELWQIHRN AFRHSELFPP FAKGTVFGRF DKTVIEERRQ CAEDLLQFSA NIPALYNSRQ 130 140 150 160 170 180 LQDFFKGGVI SDGSELIGPA EAYPDSPANA FPECGTEGFS SDSDLLSLTV DADSLAEVDD 190 200 210 220 230 240 GMASRQGSPS RTFGLSLSTD SSAVGAVASD SEPSRVEDRE SRSLFPSSLK PRLGRRDYLE 250 260 270 280 290 300 KAGELIKLAL KKEEEDDYEA ASDFYRKGVD LLLEGVQGES SPTRREAVKR RTAEYLMRAE 310 320 330 340 350 360 SICSLRAAPQ LHTGPQVLLV MDTRTEQTFI LKGLRKSSEC SRNRKTIIPR CVPNMVCLHT 370 380 390 400 410 420 YIISEESVFL VLQRAEGGKL WSYISKFLNR SSQESLDIKE GRPSMPPRVC LQQPSASPQG 430 440 450 460 470 480 GSSFESRGSD TGSMLKALPL KTSLTPSSQD DSNQEDDGQP SSPKWLDSGS SSEDECTAGY 490 500 510 520 530 540 LTLCNEYGQE KMDLVSLSEE SVMQPEGDKA DTQAVSSPAS LATGSVSPST HLRVFSGGED 550 560 570 580 590 600 LEAVSSPPTS ESLSRSKNSP MEFFRIDSKD STSELLGLDF GEKLHSLKPE PLKALFTLED 610 620 630 640 650 660 GDSPSQSLDP GESKRESEAQ DSVSRGSDDS VPVISFKEAA AEAISGAEEG RPDLLVNLPG 670 680 690 700 710 720 ELQPTKEASA MDPKFSQASA GRLDSKLLEA PDVLCLRLSS EQCHGLGPEG PEELSDPTEF 730 740 750 760 770 780 CPGGVIPEHD AQADPGVLFE AAVDHRSSPD QFLFSSLRSE SDRLGQVEVV VTAQALQESL 790 800 810 820 830 840 FHISSPCSGA NKEHSAYADT ATSEEVLLFT EPTKEEANSL FQRGSEAQER GVGAGEADKE 850 860 870 880 890 900 IHQIFEDLDK RLAASSRFFI PEGCIQRWAA EMVVALDALH REGIVCRDLN PNNILLNDGG 910 QELSQMHFFL FAVASN
	Isoform 4 [UniParc]. Checksum: EE08EE484A7603DD	FASTA	336	37,815
	10 20 30 40 50 60 MTSPWGHSAD LARFYTVTEP QRHPRGYTVY KVTARVVSRR NPEDVQEIIV WRRYSDFKKL 70 80 90 100 110 120 HRELWQIHRN AFRHSELFPP FAKGTVFGRF DKTVIEERRQ CAEDLLQFSA NIPALYNSRQ 130 140 150 160 170 180 LQDFFKGGVI SDGSELIGPA EAYPDSPANA FPECGTEGFS SDSDLLSLTV DADSLAEVDD 190 200 210 220 230 240 GMASRQGSPS RTFGLSLSTD SSAVGAVASD SEPSRVEDRE SRSLFPSSLK PRLGRRDYLE 250 260 270 280 290 300 KAGELIKLAL KKEEEDDYEA ASDFYRKGVD LLLEGVQGKV RQCHHWLESL GSLQKSERNL 310 320 330 EKPSHTRAQQ DIASPLVTKI TKSHHLIICR SVHSSK

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 4), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 284-1056 (ISOFORM 3). Strain: C57BL/6J. Tissue: Bone marrow, Cerebellum, Ovary and Uterus.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 436-1056 (ISOFORM 1). Strain: C57BL/6. Tissue: Brain and Cerebellum.
[3]	"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-577, MASS SPECTROMETRY. Tissue: Liver.
[4]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-423 AND SER-634, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK030313 mRNA. Translation: BAC26894.1. AK044809 mRNA. Translation: BAC32101.1. AK049003 mRNA. Translation: BAC33510.1. Different initiation. AK149858 mRNA. Translation: BAE29128.1. BC058403 mRNA. Translation: AAH58403.1.
IPI	IPI00223078. IPI00268901. IPI00308854. IPI00750491.
RefSeq	NP_848890.3. NM_178775.4.
UniGene	Mm.220912.
3D structure databases
ProteinModelPortal	Q8BLK9.
SMR	Q8BLK9. Positions 239-305, 878-1056.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000061769.
PTM databases
PhosphoSite	Q8BLK9.
Proteomic databases
PaxDb	Q8BLK9.
PRIDE	Q8BLK9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000159624; ENSMUSP00000125010; ENSMUSG00000089872.
GeneID	320119.
KEGG	mmu:320119.
UCSC	uc007ebf.1. mouse. uc007ebg.1. mouse. uc007ebh.2. mouse.
Organism-specific databases
CTD	26750.
MGI	MGI:2443419. Rps6kc1.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00530000063268.
HOGENOM	HOG000049101.
HOVERGEN	HBG078266.
InParanoid	Q8BLK9.
OrthoDB	EOG4TXBR9.
Gene expression databases
ArrayExpress	Q8BLK9.
Bgee	Q8BLK9.
CleanEx	MM_RPS6KC1.
Genevestigator	Q8BLK9.
Family and domain databases
Gene3D	3.30.1520.10. 1 hit.
InterPro	IPR011009. Kinase-like_dom. IPR007330. MIT. IPR001683. Phox. IPR000719. Prot_kinase_cat_dom. [Graphical view]
Pfam	PF04212. MIT. 1 hit. PF00069. Pkinase. 1 hit. PF00787. PX. 1 hit. [Graphical view]
SMART	SM00745. MIT. 1 hit. SM00312. PX. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit. SSF64268. PX. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. False negative. PS50195. PX. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	RPS6KC1. mouse.
NextBio	396068.
SOURCE	Search...

Entry information

Entry name

KS6C1_MOUSE

Accession

Primary (citable) accession number: Q8BLK9
Secondary accession number(s): Q3UDY3 , Q6PDY4, Q8BMQ5, Q8BX49

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	May 2, 2006
Last modified:	May 1, 2013
This is version 99 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program