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Q8BLK9 (KS6C1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribosomal protein S6 kinase delta-1

Short name=S6K-delta-1
EC=2.7.11.1
Alternative name(s):
52 kDa ribosomal protein S6 kinase
Gene names
Name:Rps6kc1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1056 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in transmitting sphingosine-1 phosphate (SPP)-mediated signaling into the cell By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subunit structure

Interacts with SPHK1 and phosphatidylinositol 3-phosphate By similarity.

Subcellular location

Cytoplasm. Membrane. Note: Also found in some small dot-like or ring-shaped early endosome structures By similarity.

Domain

The first protein kinase domain appears to be a pseudokinase domain as it does not contain the classical characteristics, such as the ATP-binding motif, ATP-binding site and active site.

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. S6 kinase subfamily.

Contains 1 MIT domain.

Contains 2 protein kinase domains.

Contains 1 PX (phox homology) domain.

Sequence caution

The sequence BAC33510.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
   Cellular componentCytoplasm
Membrane
   Coding sequence diversityAlternative splicing
   DomainRepeat
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphatidylinositol binding

Inferred from electronic annotation. Source: InterPro

protein serine/threonine kinase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8BLK9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform 2 (identifier: Q8BLK9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     36-47: Missing.
     89-158: Missing.
     961-961: E → G
     962-1056: Missing.
Note: Due to a partial intron retention. No experimental confirmation available.
Isoform 3 (identifier: Q8BLK9-3)

The sequence of this isoform differs from the canonical sequence as follows:
     317-347: Missing.
     932-947: HIQLTYFSRWSEVEDS → QELSQMHFFLFAVASN
     948-1056: Missing.
Note: Due to a partial intron retention. No experimental confirmation available.
Isoform 4 (identifier: Q8BLK9-4)

The sequence of this isoform differs from the canonical sequence as follows:
     279-336: ESSPTRREAV...WSLRSPAEEL → KVRQCHHWLE...IICRSVHSSK
     337-1056: Missing.
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10561056Ribosomal protein S6 kinase delta-1
PRO_0000233128

Regions

Domain8 – 132125PX
Domain276 – 30429MIT
Domain343 – 444102Protein kinase 1
Domain789 – 1046258Protein kinase 2
Nucleotide binding795 – 8039ATP By similarity

Sites

Active site9191Proton acceptor By similarity
Binding site8231ATP By similarity

Amino acid modifications

Modified residue4221Phosphoserine By similarity
Modified residue4231Phosphoserine Ref.3
Modified residue4261Phosphoserine Ref.3
Modified residue5991Phosphoserine Ref.3

Natural variations

Alternative sequence36 – 4712Missing in isoform 2.
VSP_018043
Alternative sequence89 – 15870Missing in isoform 2.
VSP_018044
Alternative sequence279 – 33658ESSPT…PAEEL → KVRQCHHWLESLGSLQKSER NLEKPSHTRAQQDIASPLVT KITKSHHLIICRSVHSSK in isoform 4.
VSP_018045
Alternative sequence317 – 34731Missing in isoform 3.
VSP_018046
Alternative sequence337 – 1056720Missing in isoform 4.
VSP_018047
Alternative sequence932 – 94716HIQLT…EVEDS → QELSQMHFFLFAVASN in isoform 3.
VSP_018048
Alternative sequence948 – 1056109Missing in isoform 3.
VSP_018049
Alternative sequence9611E → G in isoform 2.
VSP_018050
Alternative sequence962 – 105695Missing in isoform 2.
VSP_018051

Experimental info

Sequence conflict855 – 8573GSE → DSD in AAH58403. Ref.2
Sequence conflict8551G → D in BAC33510. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 2, 2006. Version 2.
Checksum: 315E6BB9926E4F42

FASTA1,056115,712
        10         20         30         40         50         60 
MTSPWGHSAD LARFYTVTEP QRHPRGYTVY KVTARVVSRR NPEDVQEIIV WRRYSDFKKL 

        70         80         90        100        110        120 
HRELWQIHRN AFRHSELFPP FAKGTVFGRF DKTVIEERRQ CAEDLLQFSA NIPALYNSRQ 

       130        140        150        160        170        180 
LQDFFKGGVI SDGSELIGPA EAYPDSPANA FPECGTEGFS SDSDLLSLTV DADSLAEVDD 

       190        200        210        220        230        240 
GMASRQGSPS RTFGLSLSTD SSAVGAVASD SEPSRVEDRE SRSLFPSSLK PRLGRRDYLE 

       250        260        270        280        290        300 
KAGELIKLAL KKEEEDDYEA ASDFYRKGVD LLLEGVQGES SPTRREAVKR RTAEYLMRAE 

       310        320        330        340        350        360 
SICSLRAAPQ LHTGPQPPGS LSSRPPWSLR SPAEELKAFR VLGVIDKVLL VMDTRTEQTF 

       370        380        390        400        410        420 
ILKGLRKSSE CSRNRKTIIP RCVPNMVCLH TYIISEESVF LVLQRAEGGK LWSYISKFLN 

       430        440        450        460        470        480 
RSSQESLDIK EGRPSMPPRV CLQQPSASPQ GGSSFESRGS DTGSMLKALP LKTSLTPSSQ 

       490        500        510        520        530        540 
DDSNQEDDGQ PSSPKWLDSG SSSEDECTAG YLTLCNEYGQ EKMDLVSLSE ESVMQPEGDK 

       550        560        570        580        590        600 
ADTQAVSSPA SLATGSVSPS THLRVFSGGE DLEAVSSPPT SESLSRSKNS PMEFFRIDSK 

       610        620        630        640        650        660 
DSTSELLGLD FGEKLHSLKP EPLKALFTLE DGDSPSQSLD PGESKRESEA QDSVSRGSDD 

       670        680        690        700        710        720 
SVPVISFKEA AAEAISGAEE GRPDLLVNLP GELQPTKEAS AMDPKFSQAS AGRLDSKLLE 

       730        740        750        760        770        780 
APDVLCLRLS SEQCHGLGPE GPEELSDPTE FCPGGVIPEH DAQADPGVLF EAAVDHRSSP 

       790        800        810        820        830        840 
DQFLFSSLRS ESDRLGQVEV VVTAQALQES LFHISSPCSG ANKEHSAYAD TATSEEVLLF 

       850        860        870        880        890        900 
TEPTKEEANS LFQRGSEAQE RGVGAGEADK EIHQIFEDLD KRLAASSRFF IPEGCIQRWA 

       910        920        930        940        950        960 
AEMVVALDAL HREGIVCRDL NPNNILLNDG GHIQLTYFSR WSEVEDSCDS DAVARMYCAP 

       970        980        990       1000       1010       1020 
EVGAVTEETE ACDWWSLGAV LFELLTGKTL VECHPAGINT HTTLNMPGCV SEEARSLIQQ 

      1030       1040       1050 
LLQFNPMERL GAGVAGVEDI KSHPFFTPVD WAELTR 

« Hide

Isoform 2 [UniParc].

Checksum: 6B5E259A161D99B8
Show »

FASTA87996,202
Isoform 3 [UniParc].

Checksum: 329F9AC0A327F228
Show »

FASTA916100,333
Isoform 4 [UniParc].

Checksum: EE08EE484A7603DD
Show »

FASTA33637,815

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 4), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 284-1056 (ISOFORM 3).
Strain: C57BL/6J.
Tissue: Bone marrow, Cerebellum, Ovary and Uterus.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 436-1056 (ISOFORM 1).
Strain: C57BL/6.
Tissue: Brain and Cerebellum.
[3]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-423; SER-426 AND SER-599, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[4]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK030313 mRNA. Translation: BAC26894.1.
AK044809 mRNA. Translation: BAC32101.1.
AK049003 mRNA. Translation: BAC33510.1. Different initiation.
AK149858 mRNA. Translation: BAE29128.1.
BC058403 mRNA. Translation: AAH58403.1.
RefSeqNP_848890.3. NM_178775.4.
UniGeneMm.220912.

3D structure databases

ProteinModelPortalQ8BLK9.
SMRQ8BLK9. Positions 878-1056.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000061769.

PTM databases

PhosphoSiteQ8BLK9.

Proteomic databases

PaxDbQ8BLK9.
PRIDEQ8BLK9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000159624; ENSMUSP00000125010; ENSMUSG00000089872. [Q8BLK9-4]
GeneID320119.
KEGGmmu:320119.

Organism-specific databases

CTD26750.
MGIMGI:2443419. Rps6kc1.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00530000063268.
HOGENOMHOG000049101.
HOVERGENHBG078266.
InParanoidQ8BLK9.
PhylomeDBQ8BLK9.

Gene expression databases

ArrayExpressQ8BLK9.
BgeeQ8BLK9.
CleanExMM_RPS6KC1.
GenevestigatorQ8BLK9.

Family and domain databases

Gene3D1.20.58.280. 1 hit.
3.30.1520.10. 1 hit.
InterProIPR011009. Kinase-like_dom.
IPR007330. MIT.
IPR001683. Phox.
IPR000719. Prot_kinase_dom.
[Graphical view]
PfamPF04212. MIT. 1 hit.
PF00069. Pkinase. 1 hit.
PF00787. PX. 1 hit.
[Graphical view]
SMARTSM00745. MIT. 1 hit.
SM00312. PX. 1 hit.
[Graphical view]
SUPFAMSSF116846. SSF116846. 1 hit.
SSF56112. SSF56112. 2 hits.
SSF64268. SSF64268. 1 hit.
PROSITEPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS50195. PX. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSRPS6KC1. mouse.
NextBio396068.
PROQ8BLK9.
SOURCESearch...

Entry information

Entry nameKS6C1_MOUSE
AccessionPrimary (citable) accession number: Q8BLK9
Secondary accession number(s): Q3UDY3 expand/collapse secondary AC list , Q6PDY4, Q8BMQ5, Q8BX49
Entry history
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: May 2, 2006
Last modified: April 16, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot