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Protein

Ribosomal protein S6 kinase delta-1

Gene

Rps6kc1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in transmitting sphingosine-1 phosphate (SPP)-mediated signaling into the cell.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei823 – 8231ATPPROSITE-ProRule annotation
Active sitei919 – 9191Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi795 – 8039ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. phosphatidylinositol binding Source: InterPro
  3. protein serine/threonine kinase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal protein S6 kinase delta-1 (EC:2.7.11.1)
Short name:
S6K-delta-1
Alternative name(s):
52 kDa ribosomal protein S6 kinase
Gene namesi
Name:Rps6kc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:2443419. Rps6kc1.

Subcellular locationi

Cytoplasm. Membrane
Note: Also found in some small dot-like or ring-shaped early endosome structures.By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10561056Ribosomal protein S6 kinase delta-1PRO_0000233128Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei422 – 4221PhosphoserineBy similarity
Modified residuei423 – 4231Phosphoserine1 Publication
Modified residuei426 – 4261Phosphoserine1 Publication
Modified residuei599 – 5991Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8BLK9.
PaxDbiQ8BLK9.
PRIDEiQ8BLK9.

PTM databases

PhosphoSiteiQ8BLK9.

Expressioni

Gene expression databases

BgeeiQ8BLK9.
CleanExiMM_RPS6KC1.
ExpressionAtlasiQ8BLK9. baseline and differential.
GenevestigatoriQ8BLK9.

Interactioni

Subunit structurei

Interacts with SPHK1 and phosphatidylinositol 3-phosphate.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000061769.

Structurei

3D structure databases

ProteinModelPortaliQ8BLK9.
SMRiQ8BLK9. Positions 853-1056.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 132125PXPROSITE-ProRule annotationAdd
BLAST
Domaini276 – 30429MITAdd
BLAST
Domaini343 – 444102Protein kinase 1PROSITE-ProRule annotationAdd
BLAST
Domaini789 – 1046258Protein kinase 2PROSITE-ProRule annotationAdd
BLAST

Domaini

The first protein kinase domain appears to be a pseudokinase domain as it does not contain the classical characteristics, such as the ATP-binding motif, ATP-binding site and active site.

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. S6 kinase subfamily.PROSITE-ProRule annotation
Contains 1 MIT domain.Curated
Contains 2 protein kinase domains.PROSITE-ProRule annotation
Contains 1 PX (phox homology) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00530000063268.
HOGENOMiHOG000049101.
HOVERGENiHBG078266.
InParanoidiQ8BLK9.
PhylomeDBiQ8BLK9.

Family and domain databases

Gene3Di1.20.58.280. 1 hit.
3.30.1520.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR007330. MIT.
IPR001683. Phox.
IPR000719. Prot_kinase_dom.
[Graphical view]
PfamiPF04212. MIT. 1 hit.
PF00069. Pkinase. 1 hit.
PF00787. PX. 1 hit.
[Graphical view]
SMARTiSM00745. MIT. 1 hit.
SM00312. PX. 1 hit.
[Graphical view]
SUPFAMiSSF116846. SSF116846. 1 hit.
SSF56112. SSF56112. 2 hits.
SSF64268. SSF64268. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS50195. PX. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8BLK9-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTSPWGHSAD LARFYTVTEP QRHPRGYTVY KVTARVVSRR NPEDVQEIIV
60 70 80 90 100
WRRYSDFKKL HRELWQIHRN AFRHSELFPP FAKGTVFGRF DKTVIEERRQ
110 120 130 140 150
CAEDLLQFSA NIPALYNSRQ LQDFFKGGVI SDGSELIGPA EAYPDSPANA
160 170 180 190 200
FPECGTEGFS SDSDLLSLTV DADSLAEVDD GMASRQGSPS RTFGLSLSTD
210 220 230 240 250
SSAVGAVASD SEPSRVEDRE SRSLFPSSLK PRLGRRDYLE KAGELIKLAL
260 270 280 290 300
KKEEEDDYEA ASDFYRKGVD LLLEGVQGES SPTRREAVKR RTAEYLMRAE
310 320 330 340 350
SICSLRAAPQ LHTGPQPPGS LSSRPPWSLR SPAEELKAFR VLGVIDKVLL
360 370 380 390 400
VMDTRTEQTF ILKGLRKSSE CSRNRKTIIP RCVPNMVCLH TYIISEESVF
410 420 430 440 450
LVLQRAEGGK LWSYISKFLN RSSQESLDIK EGRPSMPPRV CLQQPSASPQ
460 470 480 490 500
GGSSFESRGS DTGSMLKALP LKTSLTPSSQ DDSNQEDDGQ PSSPKWLDSG
510 520 530 540 550
SSSEDECTAG YLTLCNEYGQ EKMDLVSLSE ESVMQPEGDK ADTQAVSSPA
560 570 580 590 600
SLATGSVSPS THLRVFSGGE DLEAVSSPPT SESLSRSKNS PMEFFRIDSK
610 620 630 640 650
DSTSELLGLD FGEKLHSLKP EPLKALFTLE DGDSPSQSLD PGESKRESEA
660 670 680 690 700
QDSVSRGSDD SVPVISFKEA AAEAISGAEE GRPDLLVNLP GELQPTKEAS
710 720 730 740 750
AMDPKFSQAS AGRLDSKLLE APDVLCLRLS SEQCHGLGPE GPEELSDPTE
760 770 780 790 800
FCPGGVIPEH DAQADPGVLF EAAVDHRSSP DQFLFSSLRS ESDRLGQVEV
810 820 830 840 850
VVTAQALQES LFHISSPCSG ANKEHSAYAD TATSEEVLLF TEPTKEEANS
860 870 880 890 900
LFQRGSEAQE RGVGAGEADK EIHQIFEDLD KRLAASSRFF IPEGCIQRWA
910 920 930 940 950
AEMVVALDAL HREGIVCRDL NPNNILLNDG GHIQLTYFSR WSEVEDSCDS
960 970 980 990 1000
DAVARMYCAP EVGAVTEETE ACDWWSLGAV LFELLTGKTL VECHPAGINT
1010 1020 1030 1040 1050
HTTLNMPGCV SEEARSLIQQ LLQFNPMERL GAGVAGVEDI KSHPFFTPVD

WAELTR

Note: No experimental confirmation available.

Length:1,056
Mass (Da):115,712
Last modified:May 2, 2006 - v2
Checksum:i315E6BB9926E4F42
GO
Isoform 2 (identifier: Q8BLK9-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     36-47: Missing.
     89-158: Missing.
     961-961: E → G
     962-1056: Missing.

Note: Due to a partial intron retention. No experimental confirmation available.

Show »
Length:879
Mass (Da):96,202
Checksum:i6B5E259A161D99B8
GO
Isoform 3 (identifier: Q8BLK9-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     317-347: Missing.
     932-947: HIQLTYFSRWSEVEDS → QELSQMHFFLFAVASN
     948-1056: Missing.

Note: Due to a partial intron retention. No experimental confirmation available.

Show »
Length:916
Mass (Da):100,333
Checksum:i329F9AC0A327F228
GO
Isoform 4 (identifier: Q8BLK9-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     279-336: ESSPTRREAV...WSLRSPAEEL → KVRQCHHWLE...IICRSVHSSK
     337-1056: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.

Show »
Length:336
Mass (Da):37,815
Checksum:iEE08EE484A7603DD
GO

Sequence cautioni

The sequence BAC33510.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti855 – 8573GSE → DSD in AAH58403. (PubMed:15489334)Curated
Sequence conflicti855 – 8551G → D in BAC33510. (PubMed:16141072)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei36 – 4712Missing in isoform 2. 1 PublicationVSP_018043Add
BLAST
Alternative sequencei89 – 15870Missing in isoform 2. 1 PublicationVSP_018044Add
BLAST
Alternative sequencei279 – 33658ESSPT…PAEEL → KVRQCHHWLESLGSLQKSER NLEKPSHTRAQQDIASPLVT KITKSHHLIICRSVHSSK in isoform 4. 1 PublicationVSP_018045Add
BLAST
Alternative sequencei317 – 34731Missing in isoform 3. 1 PublicationVSP_018046Add
BLAST
Alternative sequencei337 – 1056720Missing in isoform 4. 1 PublicationVSP_018047Add
BLAST
Alternative sequencei932 – 94716HIQLT…EVEDS → QELSQMHFFLFAVASN in isoform 3. 1 PublicationVSP_018048Add
BLAST
Alternative sequencei948 – 1056109Missing in isoform 3. 1 PublicationVSP_018049Add
BLAST
Alternative sequencei961 – 9611E → G in isoform 2. 1 PublicationVSP_018050
Alternative sequencei962 – 105695Missing in isoform 2. 1 PublicationVSP_018051Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030313 mRNA. Translation: BAC26894.1.
AK044809 mRNA. Translation: BAC32101.1.
AK049003 mRNA. Translation: BAC33510.1. Different initiation.
AK149858 mRNA. Translation: BAE29128.1.
BC058403 mRNA. Translation: AAH58403.1.
RefSeqiNP_848890.3. NM_178775.4.
UniGeneiMm.220912.

Genome annotation databases

EnsembliENSMUST00000159624; ENSMUSP00000125010; ENSMUSG00000089872. [Q8BLK9-4]
GeneIDi320119.
KEGGimmu:320119.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030313 mRNA. Translation: BAC26894.1.
AK044809 mRNA. Translation: BAC32101.1.
AK049003 mRNA. Translation: BAC33510.1. Different initiation.
AK149858 mRNA. Translation: BAE29128.1.
BC058403 mRNA. Translation: AAH58403.1.
RefSeqiNP_848890.3. NM_178775.4.
UniGeneiMm.220912.

3D structure databases

ProteinModelPortaliQ8BLK9.
SMRiQ8BLK9. Positions 853-1056.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000061769.

PTM databases

PhosphoSiteiQ8BLK9.

Proteomic databases

MaxQBiQ8BLK9.
PaxDbiQ8BLK9.
PRIDEiQ8BLK9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000159624; ENSMUSP00000125010; ENSMUSG00000089872. [Q8BLK9-4]
GeneIDi320119.
KEGGimmu:320119.

Organism-specific databases

CTDi26750.
MGIiMGI:2443419. Rps6kc1.

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00530000063268.
HOGENOMiHOG000049101.
HOVERGENiHBG078266.
InParanoidiQ8BLK9.
PhylomeDBiQ8BLK9.

Miscellaneous databases

ChiTaRSiRps6kc1. mouse.
NextBioi396068.
PROiQ8BLK9.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BLK9.
CleanExiMM_RPS6KC1.
ExpressionAtlasiQ8BLK9. baseline and differential.
GenevestigatoriQ8BLK9.

Family and domain databases

Gene3Di1.20.58.280. 1 hit.
3.30.1520.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR007330. MIT.
IPR001683. Phox.
IPR000719. Prot_kinase_dom.
[Graphical view]
PfamiPF04212. MIT. 1 hit.
PF00069. Pkinase. 1 hit.
PF00787. PX. 1 hit.
[Graphical view]
SMARTiSM00745. MIT. 1 hit.
SM00312. PX. 1 hit.
[Graphical view]
SUPFAMiSSF116846. SSF116846. 1 hit.
SSF56112. SSF56112. 2 hits.
SSF64268. SSF64268. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS50195. PX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 4), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 284-1056 (ISOFORM 3).
    Strain: C57BL/6J.
    Tissue: Bone marrow, Cerebellum, Ovary and Uterus.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 436-1056 (ISOFORM 1).
    Strain: C57BL/6.
    Tissue: Brain and Cerebellum.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-423; SER-426 AND SER-599, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  4. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiKS6C1_MOUSE
AccessioniPrimary (citable) accession number: Q8BLK9
Secondary accession number(s): Q3UDY3
, Q6PDY4, Q8BMQ5, Q8BX49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: May 2, 2006
Last modified: January 7, 2015
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.