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Q8BLK9

- KS6C1_MOUSE

UniProt

Q8BLK9 - KS6C1_MOUSE

Protein

Ribosomal protein S6 kinase delta-1

Gene

Rps6kc1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 2 (02 May 2006)
      Previous versions | rss
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    Functioni

    May be involved in transmitting sphingosine-1 phosphate (SPP)-mediated signaling into the cell.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei823 – 8231ATPPROSITE-ProRule annotation
    Active sitei919 – 9191Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi795 – 8039ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. phosphatidylinositol binding Source: InterPro
    3. protein serine/threonine kinase activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribosomal protein S6 kinase delta-1 (EC:2.7.11.1)
    Short name:
    S6K-delta-1
    Alternative name(s):
    52 kDa ribosomal protein S6 kinase
    Gene namesi
    Name:Rps6kc1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:2443419. Rps6kc1.

    Subcellular locationi

    Cytoplasm. Membrane
    Note: Also found in some small dot-like or ring-shaped early endosome structures.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10561056Ribosomal protein S6 kinase delta-1PRO_0000233128Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei422 – 4221PhosphoserineBy similarity
    Modified residuei423 – 4231Phosphoserine1 Publication
    Modified residuei426 – 4261Phosphoserine1 Publication
    Modified residuei599 – 5991Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ8BLK9.
    PaxDbiQ8BLK9.
    PRIDEiQ8BLK9.

    PTM databases

    PhosphoSiteiQ8BLK9.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8BLK9.
    BgeeiQ8BLK9.
    CleanExiMM_RPS6KC1.
    GenevestigatoriQ8BLK9.

    Interactioni

    Subunit structurei

    Interacts with SPHK1 and phosphatidylinositol 3-phosphate.By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000061769.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BLK9.
    SMRiQ8BLK9. Positions 850-1056.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini8 – 132125PXPROSITE-ProRule annotationAdd
    BLAST
    Domaini276 – 30429MITAdd
    BLAST
    Domaini343 – 444102Protein kinase 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini789 – 1046258Protein kinase 2PROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The first protein kinase domain appears to be a pseudokinase domain as it does not contain the classical characteristics, such as the ATP-binding motif, ATP-binding site and active site.

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. S6 kinase subfamily.PROSITE-ProRule annotation
    Contains 1 MIT domain.Curated
    Contains 2 protein kinase domains.PROSITE-ProRule annotation
    Contains 1 PX (phox homology) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00530000063268.
    HOGENOMiHOG000049101.
    HOVERGENiHBG078266.
    InParanoidiQ8BLK9.
    OMAiIVILKIN.
    PhylomeDBiQ8BLK9.

    Family and domain databases

    Gene3Di1.20.58.280. 1 hit.
    3.30.1520.10. 1 hit.
    InterProiIPR011009. Kinase-like_dom.
    IPR007330. MIT.
    IPR001683. Phox.
    IPR000719. Prot_kinase_dom.
    [Graphical view]
    PfamiPF04212. MIT. 1 hit.
    PF00069. Pkinase. 1 hit.
    PF00787. PX. 1 hit.
    [Graphical view]
    SMARTiSM00745. MIT. 1 hit.
    SM00312. PX. 1 hit.
    [Graphical view]
    SUPFAMiSSF116846. SSF116846. 1 hit.
    SSF56112. SSF56112. 2 hits.
    SSF64268. SSF64268. 1 hit.
    PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS50195. PX. 1 hit.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8BLK9-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTSPWGHSAD LARFYTVTEP QRHPRGYTVY KVTARVVSRR NPEDVQEIIV     50
    WRRYSDFKKL HRELWQIHRN AFRHSELFPP FAKGTVFGRF DKTVIEERRQ 100
    CAEDLLQFSA NIPALYNSRQ LQDFFKGGVI SDGSELIGPA EAYPDSPANA 150
    FPECGTEGFS SDSDLLSLTV DADSLAEVDD GMASRQGSPS RTFGLSLSTD 200
    SSAVGAVASD SEPSRVEDRE SRSLFPSSLK PRLGRRDYLE KAGELIKLAL 250
    KKEEEDDYEA ASDFYRKGVD LLLEGVQGES SPTRREAVKR RTAEYLMRAE 300
    SICSLRAAPQ LHTGPQPPGS LSSRPPWSLR SPAEELKAFR VLGVIDKVLL 350
    VMDTRTEQTF ILKGLRKSSE CSRNRKTIIP RCVPNMVCLH TYIISEESVF 400
    LVLQRAEGGK LWSYISKFLN RSSQESLDIK EGRPSMPPRV CLQQPSASPQ 450
    GGSSFESRGS DTGSMLKALP LKTSLTPSSQ DDSNQEDDGQ PSSPKWLDSG 500
    SSSEDECTAG YLTLCNEYGQ EKMDLVSLSE ESVMQPEGDK ADTQAVSSPA 550
    SLATGSVSPS THLRVFSGGE DLEAVSSPPT SESLSRSKNS PMEFFRIDSK 600
    DSTSELLGLD FGEKLHSLKP EPLKALFTLE DGDSPSQSLD PGESKRESEA 650
    QDSVSRGSDD SVPVISFKEA AAEAISGAEE GRPDLLVNLP GELQPTKEAS 700
    AMDPKFSQAS AGRLDSKLLE APDVLCLRLS SEQCHGLGPE GPEELSDPTE 750
    FCPGGVIPEH DAQADPGVLF EAAVDHRSSP DQFLFSSLRS ESDRLGQVEV 800
    VVTAQALQES LFHISSPCSG ANKEHSAYAD TATSEEVLLF TEPTKEEANS 850
    LFQRGSEAQE RGVGAGEADK EIHQIFEDLD KRLAASSRFF IPEGCIQRWA 900
    AEMVVALDAL HREGIVCRDL NPNNILLNDG GHIQLTYFSR WSEVEDSCDS 950
    DAVARMYCAP EVGAVTEETE ACDWWSLGAV LFELLTGKTL VECHPAGINT 1000
    HTTLNMPGCV SEEARSLIQQ LLQFNPMERL GAGVAGVEDI KSHPFFTPVD 1050
    WAELTR 1056

    Note: No experimental confirmation available.

    Length:1,056
    Mass (Da):115,712
    Last modified:May 2, 2006 - v2
    Checksum:i315E6BB9926E4F42
    GO
    Isoform 2 (identifier: Q8BLK9-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         36-47: Missing.
         89-158: Missing.
         961-961: E → G
         962-1056: Missing.

    Note: Due to a partial intron retention. No experimental confirmation available.

    Show »
    Length:879
    Mass (Da):96,202
    Checksum:i6B5E259A161D99B8
    GO
    Isoform 3 (identifier: Q8BLK9-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         317-347: Missing.
         932-947: HIQLTYFSRWSEVEDS → QELSQMHFFLFAVASN
         948-1056: Missing.

    Note: Due to a partial intron retention. No experimental confirmation available.

    Show »
    Length:916
    Mass (Da):100,333
    Checksum:i329F9AC0A327F228
    GO
    Isoform 4 (identifier: Q8BLK9-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         279-336: ESSPTRREAV...WSLRSPAEEL → KVRQCHHWLE...IICRSVHSSK
         337-1056: Missing.

    Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.

    Show »
    Length:336
    Mass (Da):37,815
    Checksum:iEE08EE484A7603DD
    GO

    Sequence cautioni

    The sequence BAC33510.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti855 – 8573GSE → DSD in AAH58403. (PubMed:15489334)Curated
    Sequence conflicti855 – 8551G → D in BAC33510. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei36 – 4712Missing in isoform 2. 1 PublicationVSP_018043Add
    BLAST
    Alternative sequencei89 – 15870Missing in isoform 2. 1 PublicationVSP_018044Add
    BLAST
    Alternative sequencei279 – 33658ESSPT…PAEEL → KVRQCHHWLESLGSLQKSER NLEKPSHTRAQQDIASPLVT KITKSHHLIICRSVHSSK in isoform 4. 1 PublicationVSP_018045Add
    BLAST
    Alternative sequencei317 – 34731Missing in isoform 3. 1 PublicationVSP_018046Add
    BLAST
    Alternative sequencei337 – 1056720Missing in isoform 4. 1 PublicationVSP_018047Add
    BLAST
    Alternative sequencei932 – 94716HIQLT…EVEDS → QELSQMHFFLFAVASN in isoform 3. 1 PublicationVSP_018048Add
    BLAST
    Alternative sequencei948 – 1056109Missing in isoform 3. 1 PublicationVSP_018049Add
    BLAST
    Alternative sequencei961 – 9611E → G in isoform 2. 1 PublicationVSP_018050
    Alternative sequencei962 – 105695Missing in isoform 2. 1 PublicationVSP_018051Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK030313 mRNA. Translation: BAC26894.1.
    AK044809 mRNA. Translation: BAC32101.1.
    AK049003 mRNA. Translation: BAC33510.1. Different initiation.
    AK149858 mRNA. Translation: BAE29128.1.
    BC058403 mRNA. Translation: AAH58403.1.
    RefSeqiNP_848890.3. NM_178775.4.
    UniGeneiMm.220912.

    Genome annotation databases

    EnsembliENSMUST00000159624; ENSMUSP00000125010; ENSMUSG00000089872. [Q8BLK9-4]
    GeneIDi320119.
    KEGGimmu:320119.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK030313 mRNA. Translation: BAC26894.1 .
    AK044809 mRNA. Translation: BAC32101.1 .
    AK049003 mRNA. Translation: BAC33510.1 . Different initiation.
    AK149858 mRNA. Translation: BAE29128.1 .
    BC058403 mRNA. Translation: AAH58403.1 .
    RefSeqi NP_848890.3. NM_178775.4.
    UniGenei Mm.220912.

    3D structure databases

    ProteinModelPortali Q8BLK9.
    SMRi Q8BLK9. Positions 850-1056.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000061769.

    PTM databases

    PhosphoSitei Q8BLK9.

    Proteomic databases

    MaxQBi Q8BLK9.
    PaxDbi Q8BLK9.
    PRIDEi Q8BLK9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000159624 ; ENSMUSP00000125010 ; ENSMUSG00000089872 . [Q8BLK9-4 ]
    GeneIDi 320119.
    KEGGi mmu:320119.

    Organism-specific databases

    CTDi 26750.
    MGIi MGI:2443419. Rps6kc1.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00530000063268.
    HOGENOMi HOG000049101.
    HOVERGENi HBG078266.
    InParanoidi Q8BLK9.
    OMAi IVILKIN.
    PhylomeDBi Q8BLK9.

    Miscellaneous databases

    ChiTaRSi RPS6KC1. mouse.
    NextBioi 396068.
    PROi Q8BLK9.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8BLK9.
    Bgeei Q8BLK9.
    CleanExi MM_RPS6KC1.
    Genevestigatori Q8BLK9.

    Family and domain databases

    Gene3Di 1.20.58.280. 1 hit.
    3.30.1520.10. 1 hit.
    InterProi IPR011009. Kinase-like_dom.
    IPR007330. MIT.
    IPR001683. Phox.
    IPR000719. Prot_kinase_dom.
    [Graphical view ]
    Pfami PF04212. MIT. 1 hit.
    PF00069. Pkinase. 1 hit.
    PF00787. PX. 1 hit.
    [Graphical view ]
    SMARTi SM00745. MIT. 1 hit.
    SM00312. PX. 1 hit.
    [Graphical view ]
    SUPFAMi SSF116846. SSF116846. 1 hit.
    SSF56112. SSF56112. 2 hits.
    SSF64268. SSF64268. 1 hit.
    PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS50195. PX. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 4), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 284-1056 (ISOFORM 3).
      Strain: C57BL/6J.
      Tissue: Bone marrow, Cerebellum, Ovary and Uterus.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 436-1056 (ISOFORM 1).
      Strain: C57BL/6.
      Tissue: Brain and Cerebellum.
    3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-423; SER-426 AND SER-599, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    4. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiKS6C1_MOUSE
    AccessioniPrimary (citable) accession number: Q8BLK9
    Secondary accession number(s): Q3UDY3
    , Q6PDY4, Q8BMQ5, Q8BX49
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 2, 2006
    Last sequence update: May 2, 2006
    Last modified: October 1, 2014
    This is version 111 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3