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Protein

PHD finger protein 20

Gene

Phf20

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Contributes to methyllysine-dependent p53/TP53 stabilization and up-regulation after DNA damage (By similarity). Methyllysine-binding protein, component of the MOF histone acetyltransferase protein complex. Not required for maintaining the global histone H4 'Lys-16' acetylation (H4K16ac) levels or locus specific histone acetylation, but instead works downstream in transcriptional regulation of MOF target genes. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues.By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi257 – 269A.T hookAdd BLAST13
Zinc fingeri455 – 485C2H2-typePROSITE-ProRule annotationAdd BLAST31
Zinc fingeri657 – 703PHD-typeAdd BLAST47

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator, DNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-3214847 HATs acetylate histones
R-MMU-6804757 Regulation of TP53 Degradation
R-MMU-6804759 Regulation of TP53 Activity through Association with Co-factors
R-MMU-69541 Stabilization of p53

Names & Taxonomyi

Protein namesi
Recommended name:
PHD finger protein 20
Alternative name(s):
Hepatocellular carcinoma-associated antigen 58 homolog
Gene namesi
Name:Phf20
Synonyms:Hca58
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi

Organism-specific databases

MGIiMGI:2444148 Phf20

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice die shortly after birth and display a wide variety of phenotypes within the skeletal and hematopoietic systems.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi97W → A: Abolishes Methyllysine-binding. 1 Publication1
Mutagenesisi103Y → A: Abolishes Methyllysine-binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000593111 – 1010PHD finger protein 20Add BLAST1010

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi96Interchain (with C-100)By similarity
Disulfide bondi100Interchain (with C-96)By similarity
Modified residuei159PhosphoserineBy similarity1
Modified residuei841N6-acetyllysineBy similarity1
Modified residuei876PhosphoserineBy similarity1
Modified residuei878PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiQ8BLG0
PRIDEiQ8BLG0

PTM databases

iPTMnetiQ8BLG0
PhosphoSitePlusiQ8BLG0

Expressioni

Gene expression databases

BgeeiENSMUSG00000038116
CleanExiMM_PHF20
ExpressionAtlasiQ8BLG0 baseline and differential
GenevisibleiQ8BLG0 MM

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Component of the NSL complex at least composed of MOF/KAT8, KANSL1, KANSL2, KANSL3, MCRS1, PHF20, OGT1/OGT, WDR5 and HCFC1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi230779, 6 interactors
IntActiQ8BLG0, 4 interactors
STRINGi10090.ENSMUSP00000043138

Structurei

3D structure databases

ProteinModelPortaliQ8BLG0
SMRiQ8BLG0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 69Tudor 1Add BLAST66
Domaini83 – 147Tudor 2Add BLAST65

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi162 – 259Lys-richAdd BLAST98
Compositional biasi526 – 555Lys-richAdd BLAST30
Compositional biasi543 – 555Poly-LysAdd BLAST13

Domaini

The Tudor domain 2 mediates reading of dimethyl-lysine residues.
The Tudor domain 1 doesn't bind dimethyl-lysine residues, due to an atypical and occluded aromatic cage.By similarity

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri455 – 485C2H2-typePROSITE-ProRule annotationAdd BLAST31
Zinc fingeri657 – 703PHD-typeAdd BLAST47

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IMXB Eukaryota
ENOG410XX7Z LUCA
GeneTreeiENSGT00390000006451
HOGENOMiHOG000231997
HOVERGENiHBG053586
InParanoidiQ8BLG0
KOiK18402
OMAiDFSIHRR
OrthoDBiEOG091G01T4
PhylomeDBiQ8BLG0
TreeFamiTF106475

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR022255 DUF3776
IPR004092 Mbt
IPR037915 PHF20
IPR002999 Tudor
IPR019786 Zinc_finger_PHD-type_CS
IPR013087 Znf_C2H2_type
IPR011011 Znf_FYVE_PHD
IPR001965 Znf_PHD
IPR013083 Znf_RING/FYVE/PHD
PANTHERiPTHR15856:SF27 PTHR15856:SF27, 1 hit
PfamiView protein in Pfam
PF12618 DUF3776, 1 hit
PF02820 MBT, 1 hit
SMARTiView protein in SMART
SM00249 PHD, 1 hit
SM00333 TUDOR, 2 hits
SUPFAMiSSF57903 SSF57903, 1 hit
PROSITEiView protein in PROSITE
PS01359 ZF_PHD_1, 1 hit
PS00028 ZINC_FINGER_C2H2_1, 1 hit
PS50157 ZINC_FINGER_C2H2_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BLG0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTKHPPNRRG ISFEVGAQLE ARDRLKNWYP AHIEDIDYEE GRVLIHFKRW
60 70 80 90 100
NHRYDEWFCW DSPYLRPLEK IQLRKEGLHD EDGSSEFQIN QQVLACWSDC
110 120 130 140 150
RFYPARVTAV NKDGTYTVKF YDGVVQTVKH IHVKAFSKDQ NIVGNARPKE
160 170 180 190 200
TDHKSLSSSP EKREKFKEQR KVTVNVKKDK VEKALKTEKR PKQPDKEGKL
210 220 230 240 250
ICSEKGKVSE KSLPKNEKED KENISENERE YSGDAQVEKK PEKDLVKNPQ
260 270 280 290 300
ENLKEPKRKR GRPPSITPTA VDSNSQTLQP ITLELRRRKI SKRSDTPLKR
310 320 330 340 350
PRLDKNSPQE QSKKRSENSD KDLSRRRSSR LSTNGTREIL DPDSIVPDLV
360 370 380 390 400
HTVDTNPLPD KSPSAKDSAE GQLKSPLEAG QVSSALTCHP IGDGLGAADL
410 420 430 440 450
ELNCKSMGEN TMKTEPVSPL AEVQEVSTVE VPNTLKKVDD SVTLNVPAVD
460 470 480 490 500
LDHKFRCKVL DCLKFFRKAK LLHYHMKYFH GMEKSPEPEE GPGKTHVQTR
510 520 530 540 550
GSAVPDKTSQ ESLTRKRVSA SSPTAKEKEK TKEKKFKELV RVKPKKKKKK
560 570 580 590 600
KKKTKPECPC SEDISDTSQE PSPPKTFAVT RCGSSHKPGV HMSPQLHGSD
610 620 630 640 650
NGNHKGKLKT CEEDNLSESS SESFLWSDEE YGQDVDVTTN PDEELEGDDR
660 670 680 690 700
YDFEVVRCIC EVQEENDFMI QCEECQCWQH GVCMGLLEEN VPEKYTCYVC
710 720 730 740 750
QDPPGQRPGF KYWYDKEWLS RGHMHGLAFL DQNYSHQNAR KIVATHQLLG
760 770 780 790 800
DVQRVIQVLH GLQLKMSILQ SREHPDLQLW CQPWKQHSGE GRAHPRHIHI
810 820 830 840 850
TDARSEESPS YRTLNGAVEK PSPLPRSVEE SYITSEHCYQ KPRAYYPAVE
860 870 880 890 900
QRLVVETRGS ALDAAVSPLC ENGDDSLSPR LGWPIDQDRS RGDIDPKPSS
910 920 930 940 950
PKVREYISKN VLPEETPARK LLDRGGEGLV SSQHQWQFNL LTHVESLQDE
960 970 980 990 1000
VTHRMDSIEK ELDVLESWLD YTGELEPPEP LARLPQLKHC IKQLLTDLGK
1010
VQQIALCCST
Length:1,010
Mass (Da):115,280
Last modified:July 11, 2003 - v2
Checksum:i2BC32811A520342D
GO
Isoform 2 (identifier: Q8BLG0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-28: MTKHPPNRRGISFEVGAQLEARDRLKNW → GAARTVLLSVGLERRSRSGAVR

Note: Incomplete sequence. No experimental confirmation available.
Show »
Length:1,004
Mass (Da):114,284
Checksum:iF5D70ABE4C5CC87B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti817A → T in AAH11337 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0077621 – 28MTKHP…RLKNW → GAARTVLLSVGLERRSRSGA VR in isoform 2. CuratedAdd BLAST28

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK033017 mRNA Translation: BAC28129.1
AK038573 mRNA Translation: BAC30050.2
AK045309 mRNA Translation: BAC32304.1
BC011337 mRNA Translation: AAH11337.1
CCDSiCCDS38297.1 [Q8BLG0-1]
RefSeqiNP_766262.2, NM_172674.2 [Q8BLG0-1]
UniGeneiMm.427078

Genome annotation databases

EnsembliENSMUST00000037401; ENSMUSP00000043138; ENSMUSG00000038116 [Q8BLG0-1]
GeneIDi228829
KEGGimmu:228829
UCSCiuc008nmy.1 mouse [Q8BLG0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Entry informationi

Entry nameiPHF20_MOUSE
AccessioniPrimary (citable) accession number: Q8BLG0
Secondary accession number(s): Q8BMA2, Q8BYR4, Q921N1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2003
Last sequence update: July 11, 2003
Last modified: March 28, 2018
This is version 132 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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