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Q8BLF2 (CDKL3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclin-dependent kinase-like 3

EC=2.7.11.22
Gene names
Name:Cdkl3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length595 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subcellular location

Cytoplasm By similarity.

Domain

The [NKR]KIAxRE motif seems to be a cyclin-binding region.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

Ontologies

Keywords
   Cellular componentCytoplasm
   Coding sequence diversityAlternative splicing
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

cyclin-dependent protein serine/threonine kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 7 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8BLF2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8BLF2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     576-585: GGDGDCEGKN → QSARHLPGQC
     586-595: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q8BLF2-7)

The sequence of this isoform differs from the canonical sequence as follows:
     576-581: GGDGDC → SLTMYL
     582-595: Missing.
Note: Gene prediction based on EST data.
Isoform 4 (identifier: Q8BLF2-4)

The sequence of this isoform differs from the canonical sequence as follows:
     295-353: FIPELRAKLL...LYGKEVDRDK → KWTETKGPRS...HWTRSLLSWN
     354-595: Missing.
Note: No experimental confirmation available.
Isoform 5 (identifier: Q8BLF2-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-17: MEMYETLGKVGEGSYGT → MKPLEKWEREVMEP
     576-581: GGDGDC → SLTMYL
     582-595: Missing.
Note: No experimental confirmation available.
Isoform 6 (identifier: Q8BLF2-6)

The sequence of this isoform differs from the canonical sequence as follows:
     458-595: Missing.
Note: No experimental confirmation available.
Isoform 7 (identifier: Q8BLF2-8)

The sequence of this isoform differs from the canonical sequence as follows:
     576-595: GGDGDCEGKNLKRNRFFFSR → QPVKQVNSNQ
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 595595Cyclin-dependent kinase-like 3
PRO_0000085822

Regions

Domain4 – 286283Protein kinase
Nucleotide binding10 – 189ATP By similarity
Motif45 – 517[NKR]KIAxRE

Sites

Active site1251Proton acceptor By similarity
Binding site331ATP By similarity

Natural variations

Alternative sequence1 – 1717MEMYE…GSYGT → MKPLEKWEREVMEP in isoform 5.
VSP_016149
Alternative sequence295 – 35359FIPEL…VDRDK → KWTETKGPRSSKSESLRPKG AKEMSQTRRSQSMKATTASR AQLMTHSPHHWTRSLLSWN in isoform 4.
VSP_016150
Alternative sequence354 – 595242Missing in isoform 4.
VSP_016151
Alternative sequence458 – 595138Missing in isoform 6.
VSP_016152
Alternative sequence576 – 59520GGDGD…FFFSR → QPVKQVNSNQ in isoform 7.
VSP_030154
Alternative sequence576 – 58510GGDGDCEGKN → QSARHLPGQC in isoform 2.
VSP_016154
Alternative sequence576 – 5816GGDGDC → SLTMYL in isoform 3 and isoform 5.
VSP_016155
Alternative sequence582 – 59514Missing in isoform 3 and isoform 5.
VSP_016156
Alternative sequence586 – 59510Missing in isoform 2.
VSP_016157

Experimental info

Sequence conflict121E → Q in BAC32701. Ref.1
Sequence conflict231H → Y in BAC37400. Ref.1
Sequence conflict1531P → H in BAC32324. Ref.1
Sequence conflict3521D → E in BAC37400. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 22, 2005. Version 2.
Checksum: 3DB415A3EEBC7C4B

FASTA59567,739
        10         20         30         40         50         60 
MEMYETLGKV GEGSYGTVMK CKHKDTGRIV AIKIFYEKPE KSVNKIATRE IKFLKQFRHE 

        70         80         90        100        110        120 
NLVNLIEVFR QKKKIHLVFE FIDHTVLDEL QHYCHGLESK RLRKYLFQIL RAIEYLHNNN 

       130        140        150        160        170        180 
IIHRDIKPEN ILVSQSGITK LCDFGFARTL AAPGDVYTDY VATRWYRAPE LVLKDTSYGK 

       190        200        210        220        230        240 
PVDIWALGCM IIEMATGHPF LPSSSDLDLL HKIVLKVGNL TPHLHNIFSK SPIFAGVVLP 

       250        260        270        280        290        300 
QVQHPKTARK KYPKLNGLLA DIVHACLQID PAERTSSTDL LRHDYFTRDG FIEKFIPELR 

       310        320        330        340        350        360 
AKLLQEAKVN SFIKPKENFK ENEPVRDEKK SVFTNTLLYG NPSLYGKEVD RDKRAKELKV 

       370        380        390        400        410        420 
RVIKAKGGKG DVPDQKKPEY EGDHRQQGTA DDTQPSSLDK KPSVLELTNP LNPSENSDGV 

       430        440        450        460        470        480 
KEDPHAGGCM IMPPINLTSS NLLAANLSSN LSHPNSRLTE RTKKRRTSSQ TIGQTLSNSR 

       490        500        510        520        530        540 
QEDTGPTQVQ TEKGAFNERT GQNDQISSGN KRKLNFPKCD RKEFHFPELP FTVQAKEMKG 

       550        560        570        580        590 
MEVKQIKVLK RESKKTDSSK IPTLLSMDPN QEKQEGGDGD CEGKNLKRNR FFFSR 

« Hide

Isoform 2 [UniParc].

Checksum: 619B377FACD9486D
Show »

FASTA58566,531
Isoform 3 [UniParc].

Checksum: 9CC3EAE169D206C9
Show »

FASTA58166,162
Isoform 4 [UniParc].

Checksum: 7A645EE9FCAE2328
Show »

FASTA35340,619
Isoform 5 [UniParc].

Checksum: B62583F530F130E8
Show »

FASTA57866,112
Isoform 6 [UniParc].

Checksum: 6FCDD36F51152B61
Show »

FASTA45751,901
Isoform 7 [UniParc].

Checksum: 6126BAAC992E6F42
Show »

FASTA58566,576

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2; 5 AND 6), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 368-595 (ISOFORM 7).
Strain: C57BL/6J.
Tissue: Brain cortex, Corpora quadrigemina, Embryo and Embryonic lung.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
Strain: FVB/N.
Tissue: Kidney.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK043995 mRNA. Translation: BAC31729.2.
AK045356 mRNA. Translation: BAC32324.1.
AK046394 mRNA. Translation: BAC32701.1.
AK050990 mRNA. Translation: BAC34488.1.
AK078804 mRNA. Translation: BAC37400.1.
AL669920 Genomic DNA. Translation: CAM19001.1.
AL669920 Genomic DNA. Translation: CAM19002.1.
AL669920 Genomic DNA. Translation: CAM19003.1.
AL669920 Genomic DNA. Translation: CAM19004.1.
AL669920 Genomic DNA. Translation: CAM19005.1.
BC028871 mRNA. Translation: AAH28871.1.
RefSeqNP_001160125.1. NM_001166653.1.
NP_001160126.1. NM_001166654.1.
NP_001160127.1. NM_001166655.1.
NP_001160128.1. NM_001166656.1.
NP_001160129.1. NM_001166657.1.
NP_722480.3. NM_153785.4.
UniGeneMm.280557.

3D structure databases

ProteinModelPortalQ8BLF2.
SMRQ8BLF2. Positions 1-354.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ8BLF2.

Proteomic databases

PaxDbQ8BLF2.
PRIDEQ8BLF2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000063303; ENSMUSP00000064315; ENSMUSG00000020389. [Q8BLF2-1]
ENSMUST00000063321; ENSMUSP00000065128; ENSMUSG00000020389. [Q8BLF2-7]
ENSMUST00000109076; ENSMUSP00000104704; ENSMUSG00000020389. [Q8BLF2-4]
ENSMUST00000109077; ENSMUSP00000104705; ENSMUSG00000020389. [Q8BLF2-4]
ENSMUST00000109078; ENSMUSP00000104706; ENSMUSG00000020389. [Q8BLF2-6]
ENSMUST00000109079; ENSMUSP00000104707; ENSMUSG00000020389. [Q8BLF2-7]
ENSMUST00000109081; ENSMUSP00000104709; ENSMUSG00000020389. [Q8BLF2-2]
ENSMUST00000121591; ENSMUSP00000112477; ENSMUSG00000020389. [Q8BLF2-2]
GeneID213084.
KEGGmmu:213084.
UCSCuc007iuv.2. mouse. [Q8BLF2-1]
uc007iux.2. mouse. [Q8BLF2-4]
uc007iuz.2. mouse. [Q8BLF2-2]
uc011xul.1. mouse. [Q8BLF2-7]

Organism-specific databases

CTD51265.
MGIMGI:2388268. Cdkl3.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00650000093115.
HOVERGENHBG080204.
KOK08824.
OrthoDBEOG7992PS.
TreeFamTF101031.

Gene expression databases

ArrayExpressQ8BLF2.
BgeeQ8BLF2.
CleanExMM_CDKL3.
GenevestigatorQ8BLF2.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio373855.
PROQ8BLF2.
SOURCESearch...

Entry information

Entry nameCDKL3_MOUSE
AccessionPrimary (citable) accession number: Q8BLF2
Secondary accession number(s): A2ACR7 expand/collapse secondary AC list , A2ACR8, A2ACR9, A2ACS0, A2ACS1, Q5M6W2, Q8BKR2, Q8BL49, Q8BLN9, Q8BVE0, Q8K134
Entry history
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: April 16, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot