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Q8BLF1

- NCEH1_MOUSE

UniProt

Q8BLF1 - NCEH1_MOUSE

Protein

Neutral cholesterol ester hydrolase 1

Gene

Nceh1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Hydrolyzes 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. May be responsible for cholesterol ester hydrolysis in macrophages. Also involved in organ detoxification by hydrolyzing exogenous organophosphorus compounds.4 Publications

    Enzyme regulationi

    Inhibited by bulky trifluoromethyl ketones.1 Publication

    pH dependencei

    Optimum pH is 7.2 for cholesterol ester hydrolysis.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei191 – 19111 Publication
    Active sitei348 – 34811 Publication
    Active sitei378 – 37811 Publication

    GO - Molecular functioni

    1. carboxylic ester hydrolase activity Source: InterPro
    2. phosphate ion binding Source: MGI
    3. serine hydrolase activity Source: MGI

    GO - Biological processi

    1. lipid catabolic process Source: UniProtKB-KW
    2. protein dephosphorylation Source: MGI
    3. xenobiotic metabolic process Source: MGI

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Protein family/group databases

    MEROPSiS09.992.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neutral cholesterol ester hydrolase 1 (EC:3.1.1.-)
    Short name:
    NCEH
    Alternative name(s):
    Arylacetamide deacetylase-like 1
    Chlorpyrifos oxon-binding protein
    Short name:
    CPO-BP
    Gene namesi
    Name:Nceh1
    Synonyms:Aadacl1, Kiaa1363
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:2443191. Nceh1.

    Subcellular locationi

    Membrane By similarity; Single-pass type II membrane protein By similarity. Microsome 1 Publication

    GO - Cellular componenti

    1. endoplasmic reticulum Source: UniProtKB-KW
    2. integral component of membrane Source: UniProtKB-KW
    3. membrane Source: MGI

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    Pathology & Biotechi

    Disruption phenotypei

    Mice are phenotypically normal but more sensitive to organophosphorus insecticide toxicity.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 408408Neutral cholesterol ester hydrolase 1PRO_0000265940Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi270 – 2701N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi367 – 3671N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi389 – 3891N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    N-glycosylated.1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiQ8BLF1.
    PaxDbiQ8BLF1.
    PRIDEiQ8BLF1.

    PTM databases

    PhosphoSiteiQ8BLF1.

    Expressioni

    Tissue specificityi

    Present in brain, heart, kidney, lung, spinal cord and testis but not liver (at protein level). Expressed in peritoneal macrophages and kidney.2 Publications

    Gene expression databases

    BgeeiQ8BLF1.
    CleanExiMM_AADACL1.
    GenevestigatoriQ8BLF1.

    Interactioni

    Protein-protein interaction databases

    IntActiQ8BLF1. 3 interactions.
    MINTiMINT-4113633.
    STRINGi10090.ENSMUSP00000045864.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BLF1.
    SMRiQ8BLF1. Positions 61-262, 293-385.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 44CytoplasmicSequence Analysis
    Topological domaini26 – 408383LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei5 – 2521Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi113 – 1153Involved in the stabilization of the negatively charged intermediate by the formation of the oxyanion holeBy similarity

    Sequence similaritiesi

    Belongs to the 'GDXG' lipolytic enzyme family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0657.
    GeneTreeiENSGT00550000074556.
    HOGENOMiHOG000033738.
    HOVERGENiHBG058974.
    InParanoidiQ8BLF1.
    KOiK14349.
    OMAiALGQQFT.
    OrthoDBiEOG7HB599.
    PhylomeDBiQ8BLF1.
    TreeFamiTF314978.

    Family and domain databases

    Gene3Di3.40.50.1820. 2 hits.
    InterProiIPR029058. AB_hydrolase.
    IPR013094. AB_hydrolase_3.
    IPR017157. Arylacetamide_deacetylase.
    IPR002168. Lipase_GDXG_AS.
    [Graphical view]
    PfamiPF07859. Abhydrolase_3. 2 hits.
    [Graphical view]
    PIRSFiPIRSF037251. Arylacetamide_deacetylase. 1 hit.
    SUPFAMiSSF53474. SSF53474. 2 hits.
    PROSITEiPS01174. LIPASE_GDXG_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8BLF1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRSSCVLLAA LLALAAYYVY IPLPSAVSDP WKLMLLDATF RGAQQVSNLI    50
    HSLGLNHHLI ALNFIITSFG KQSARSSPKV KVTDTDFDGV EVRVFEGSPK 100
    PEEPLRRSVI YIHGGGWALA SAKISYYDQL CTTMAEELNA VIVSIEYRLV 150
    PQVYFPEQIH DVIRATKYFL QPEVLDKYKV DPGRVGISGD SAGGNLAAAL 200
    GQQFTYVASL KNKLKLQALV YPVLQALDFN TPSYQQSMNT PILPRHVMVR 250
    YWLDYFKGNY DFVEAMIVNN HTSLDVERAA ALRARLDWTS LLPSSIKKNY 300
    KPIMQTTGNA RIVQEIPQLL DAAASPLIAE QEVLEALPKT YILTCEHDVL 350
    RDDGIMYAKR LESAGVNVTL DHFEDGFHGC MIFTSWPTNF SVGIRTRNSY 400
    IKWLDQNL 408
    Length:408
    Mass (Da):45,740
    Last modified:March 1, 2003 - v1
    Checksum:iFFEC0EA1CDCB59E7
    GO

    Sequence cautioni

    The sequence BAD32436.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti93 – 931R → Q in BAC28680. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK173158 mRNA. Translation: BAD32436.1. Different initiation.
    AK034339 mRNA. Translation: BAC28680.1.
    AK045363 mRNA. Translation: BAC32327.1.
    AK135837 mRNA. Translation: BAE22685.1.
    BC082569 mRNA. Translation: AAH82569.1.
    CCDSiCCDS17271.1.
    RefSeqiNP_848887.1. NM_178772.3.
    UniGeneiMm.24576.

    Genome annotation databases

    EnsembliENSMUST00000046515; ENSMUSP00000045864; ENSMUSG00000027698.
    GeneIDi320024.
    KEGGimmu:320024.
    UCSCiuc008otl.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK173158 mRNA. Translation: BAD32436.1 . Different initiation.
    AK034339 mRNA. Translation: BAC28680.1 .
    AK045363 mRNA. Translation: BAC32327.1 .
    AK135837 mRNA. Translation: BAE22685.1 .
    BC082569 mRNA. Translation: AAH82569.1 .
    CCDSi CCDS17271.1.
    RefSeqi NP_848887.1. NM_178772.3.
    UniGenei Mm.24576.

    3D structure databases

    ProteinModelPortali Q8BLF1.
    SMRi Q8BLF1. Positions 61-262, 293-385.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8BLF1. 3 interactions.
    MINTi MINT-4113633.
    STRINGi 10090.ENSMUSP00000045864.

    Chemistry

    BindingDBi Q8BLF1.
    ChEMBLi CHEMBL5428.

    Protein family/group databases

    MEROPSi S09.992.

    PTM databases

    PhosphoSitei Q8BLF1.

    Proteomic databases

    MaxQBi Q8BLF1.
    PaxDbi Q8BLF1.
    PRIDEi Q8BLF1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000046515 ; ENSMUSP00000045864 ; ENSMUSG00000027698 .
    GeneIDi 320024.
    KEGGi mmu:320024.
    UCSCi uc008otl.1. mouse.

    Organism-specific databases

    CTDi 57552.
    MGIi MGI:2443191. Nceh1.
    Rougei Search...

    Phylogenomic databases

    eggNOGi COG0657.
    GeneTreei ENSGT00550000074556.
    HOGENOMi HOG000033738.
    HOVERGENi HBG058974.
    InParanoidi Q8BLF1.
    KOi K14349.
    OMAi ALGQQFT.
    OrthoDBi EOG7HB599.
    PhylomeDBi Q8BLF1.
    TreeFami TF314978.

    Miscellaneous databases

    ChiTaRSi NCEH1. mouse.
    NextBioi 395889.
    PROi Q8BLF1.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8BLF1.
    CleanExi MM_AADACL1.
    Genevestigatori Q8BLF1.

    Family and domain databases

    Gene3Di 3.40.50.1820. 2 hits.
    InterProi IPR029058. AB_hydrolase.
    IPR013094. AB_hydrolase_3.
    IPR017157. Arylacetamide_deacetylase.
    IPR002168. Lipase_GDXG_AS.
    [Graphical view ]
    Pfami PF07859. Abhydrolase_3. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF037251. Arylacetamide_deacetylase. 1 hit.
    SUPFAMi SSF53474. SSF53474. 2 hits.
    PROSITEi PS01174. LIPASE_GDXG_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
      DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Embryonic intestine.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Corpora quadrigemina and Diencephalon.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Eye.
    4. "Discovering potent and selective reversible inhibitors of enzymes in complex proteomes."
      Leung D., Hardouin C., Boger D.L., Cravatt B.F.
      Nat. Biotechnol. 21:687-691(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: ENZYME REGULATION, GLYCOSYLATION.
    5. Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    6. "Serine hydrolase KIAA1363: toxicological and structural features with emphasis on organophosphate interactions."
      Nomura D.K., Durkin K.A., Chiang K.P., Quistad G.B., Cravatt B.F., Casida J.E.
      Chem. Res. Toxicol. 19:1142-1150(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ACTIVE SITE, TISSUE SPECIFICITY.
    7. Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    8. "Dual roles of brain serine hydrolase KIAA1363 in ether lipid metabolism and organophosphate detoxification."
      Nomura D.K., Fujioka K., Issa R.S., Ward A.M., Cravatt B.F., Casida J.E.
      Toxicol. Appl. Pharmacol. 228:42-48(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiNCEH1_MOUSE
    AccessioniPrimary (citable) accession number: Q8BLF1
    Secondary accession number(s): Q69ZL0, Q8BZK3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 12, 2006
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3