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Protein

Vesicular glutamate transporter 2

Gene

Slc17a6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.4 Publications

GO - Molecular functioni

  • L-glutamate transmembrane transporter activity Source: MGI
  • symporter activity Source: UniProtKB-KW

GO - Biological processi

  • L-glutamate transmembrane transport Source: GOC
  • neurotransmitter uptake Source: MGI
  • sodium ion transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Ion transport, Neurotransmitter transport, Sodium transport, Symport, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

SABIO-RKQ8BLE7.

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicular glutamate transporter 2
Short name:
VGluT2
Alternative name(s):
Differentiation-associated BNPI
Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter
Solute carrier family 17 member 6
Gene namesi
Name:Slc17a6
Synonyms:Dnpi, Vglut2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2156052. Slc17a6.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7171CytoplasmicSequence analysisAdd
BLAST
Transmembranei72 – 9221HelicalSequence analysisAdd
BLAST
Topological domaini93 – 12533VesicularSequence analysisAdd
BLAST
Transmembranei126 – 14621HelicalSequence analysisAdd
BLAST
Topological domaini147 – 1482CytoplasmicSequence analysis
Transmembranei149 – 16921HelicalSequence analysisAdd
BLAST
Topological domaini170 – 1778VesicularSequence analysis
Transmembranei178 – 19821HelicalSequence analysisAdd
BLAST
Topological domaini199 – 21618CytoplasmicSequence analysisAdd
BLAST
Transmembranei217 – 23721HelicalSequence analysisAdd
BLAST
Topological domaini238 – 2447VesicularSequence analysis
Transmembranei245 – 26521HelicalSequence analysisAdd
BLAST
Topological domaini266 – 31045CytoplasmicSequence analysisAdd
BLAST
Transmembranei311 – 33121HelicalSequence analysisAdd
BLAST
Topological domaini332 – 34918VesicularSequence analysisAdd
BLAST
Transmembranei350 – 37021HelicalSequence analysisAdd
BLAST
Topological domaini371 – 38616CytoplasmicSequence analysisAdd
BLAST
Transmembranei387 – 40721HelicalSequence analysisAdd
BLAST
Topological domaini408 – 4092VesicularSequence analysis
Transmembranei410 – 43021HelicalSequence analysisAdd
BLAST
Topological domaini431 – 44313CytoplasmicSequence analysisAdd
BLAST
Transmembranei444 – 46421HelicalSequence analysisAdd
BLAST
Topological domaini465 – 47713VesicularSequence analysisAdd
BLAST
Transmembranei478 – 49821HelicalSequence analysisAdd
BLAST
Topological domaini499 – 58284CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Membrane, Synapse, Synaptosome

Pathology & Biotechi

Disruption phenotypei

Mice exhibit an elevated rate of perinatal lethality. Surviving animals display a strong reduction in evoked glutamergic responses in thalamic neurons. Reduction of protein level in homozygous and heterozygous knockouts leads to a graded reduction in the amplitude of the postsynaptic response to single vesicle fusion in thalamic neurons, consistent with a role for this protein in determining quantal size.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 582582Vesicular glutamate transporter 2PRO_0000318170Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi100 – 1001N-linked (GlcNAc...)Sequence analysis
Glycosylationi101 – 1011N-linked (GlcNAc...)Sequence analysis
Glycosylationi470 – 4701N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ8BLE7.
PaxDbiQ8BLE7.
PRIDEiQ8BLE7.

PTM databases

iPTMnetiQ8BLE7.
PhosphoSiteiQ8BLE7.

Expressioni

Tissue specificityi

Expressed in brain. Expressed in hippocampal neurons (at protein level).4 Publications

Developmental stagei

Expressed in brain throughout development. Transiently expressed in hippocampal neurons during the first week after birth, with expression decreasing thereafter.2 Publications

Gene expression databases

BgeeiQ8BLE7.

Interactioni

Protein-protein interaction databases

IntActiQ8BLE7. 2 interactions.
MINTiMINT-3089499.
STRINGi10090.ENSMUSP00000032710.

Structurei

3D structure databases

ProteinModelPortaliQ8BLE7.
SMRiQ8BLE7. Positions 217-249.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2532. Eukaryota.
ENOG410XPWC. LUCA.
HOGENOMiHOG000230812.
HOVERGENiHBG008834.
InParanoidiQ8BLE7.
KOiK12302.
OrthoDBiEOG789C9Z.
PhylomeDBiQ8BLE7.
TreeFamiTF313535.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BLE7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESVKQRILA PGKEGIKNFA GKSLGQIYRV LEKKQDNRET IELTEDGKPL
60 70 80 90 100
EVPEKKAPLC DCTCFGLPRR YIIAIMSGLG FCISFGIRCN LGVAIVDMVN
110 120 130 140 150
NSTIHRGGKV IKEKAKFNWD PETVGMIHGS FFWGYIITQI PGGYIASRLA
160 170 180 190 200
ANRVFGAAIL LTSTLNMLIP SAARVHYGCV IFVRILQGLV EGVTYPACHG
210 220 230 240 250
IWSKWAPPLE RSRLATTSFC GSYAGAVIAM PLAGILVQYT GWSSVFYVYG
260 270 280 290 300
SFGMVWYMFW LLVSYESPAK HPTITDEERR YIEESIGESA NLLGAMEKFK
310 320 330 340 350
TPWRKFFTSM PVYAIIVANF CRSWTFYLLL ISQPAYFEEV FGFEISKVGM
360 370 380 390 400
LSAVPHLVMT IIVPIGGQIA DFLRSKQILS TTTVRKIMNC GGFGMEATLL
410 420 430 440 450
LVVGYSHTRG VAISFLVLAV GFSGFAISGF NVNHLDIAPR YASILMGISD
460 470 480 490 500
GVGTLSGMVC PIIVGAMTKN KSREEWQYVF LIAALVHYGG VIFYALFASG
510 520 530 540 550
EKQPWADPEE TSEEKCGFIH EDELDEETGD ITQNYINYGT TKSYGATSQE
560 570 580
NGGWPNGWEK KEEFVQEGAQ DAYTYKDRDD YS
Length:582
Mass (Da):64,561
Last modified:March 1, 2003 - v1
Checksum:i9F7A47C0E0C7A64D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF324864 mRNA. Translation: AAL08941.1.
AK045409 mRNA. Translation: BAC32349.1.
BC038375 mRNA. Translation: AAH38375.1.
CCDSiCCDS39969.1.
RefSeqiNP_543129.3. NM_080853.3.
UniGeneiMm.256618.

Genome annotation databases

GeneIDi140919.
KEGGimmu:140919.
UCSCiuc009hcf.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF324864 mRNA. Translation: AAL08941.1.
AK045409 mRNA. Translation: BAC32349.1.
BC038375 mRNA. Translation: AAH38375.1.
CCDSiCCDS39969.1.
RefSeqiNP_543129.3. NM_080853.3.
UniGeneiMm.256618.

3D structure databases

ProteinModelPortaliQ8BLE7.
SMRiQ8BLE7. Positions 217-249.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BLE7. 2 interactions.
MINTiMINT-3089499.
STRINGi10090.ENSMUSP00000032710.

PTM databases

iPTMnetiQ8BLE7.
PhosphoSiteiQ8BLE7.

Proteomic databases

MaxQBiQ8BLE7.
PaxDbiQ8BLE7.
PRIDEiQ8BLE7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi140919.
KEGGimmu:140919.
UCSCiuc009hcf.2. mouse.

Organism-specific databases

CTDi57084.
MGIiMGI:2156052. Slc17a6.

Phylogenomic databases

eggNOGiKOG2532. Eukaryota.
ENOG410XPWC. LUCA.
HOGENOMiHOG000230812.
HOVERGENiHBG008834.
InParanoidiQ8BLE7.
KOiK12302.
OrthoDBiEOG789C9Z.
PhylomeDBiQ8BLE7.
TreeFamiTF313535.

Enzyme and pathway databases

SABIO-RKQ8BLE7.

Miscellaneous databases

NextBioi370045.
PROiQ8BLE7.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BLE7.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular and functional analysis of a novel neuronal vesicular glutamate transporter."
    Bai L., Xu H., Collins J.F., Ghishan F.K.
    J. Biol. Chem. 276:36764-36769(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Corpora quadrigemina.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.
  4. "Molecular cloning and functional identification of mouse vesicular glutamate transporter 3 and its expression in subsets of novel excitatory neurons."
    Schaefer M.K.-H., Varoqui H., Defamie N., Weihe E., Erickson J.D.
    J. Biol. Chem. 277:50734-50748(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  5. "An essential role for vesicular glutamate transporter 1 (VGLUT1) in postnatal development and control of quantal size."
    Wojcik S.M., Rhee J.S., Herzog E., Sigler A., Jahn R., Takamori S., Brose N., Rosenmund C.
    Proc. Natl. Acad. Sci. U.S.A. 101:7158-7163(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  6. "Vesicular glutamate transporters 1 and 2 target to functionally distinct synaptic release sites."
    Fremeau R.T. Jr., Kam K., Qureshi T., Johnson J., Copenhagen D.R., Storm-Mathisen J., Chaudhry F.A., Nicoll R.A., Edwards R.H.
    Science 304:1815-1819(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  7. "Synaptic and vesicular co-localization of the glutamate transporters VGLUT1 and VGLUT2 in the mouse hippocampus."
    Herzog E., Takamori S., Jahn R., Brose N., Wojcik S.M.
    J. Neurochem. 99:1011-1018(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  8. "Vesicular glutamate transporter VGLUT2 expression levels control quantal size and neuropathic pain."
    Moechars D., Weston M.C., Leo S., Callaerts-Vegh Z., Goris I., Daneels G., Buist A., Cik M., van der Spek P., Kass S., Meert T., D'Hooge R., Rosenmund C., Hampson R.M.
    J. Neurosci. 26:12055-12066(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiVGLU2_MOUSE
AccessioniPrimary (citable) accession number: Q8BLE7
Secondary accession number(s): Q920B7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: March 1, 2003
Last modified: May 11, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

The phosphorylation site Tyr-195 is located in a predicted transmembrane region.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.