Q8BLB7 (LMBL3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lethal(3)malignant brain tumor-like protein 3
Short name=L(3)mbt-like protein 3

Alternative name(s):
MBT-1

Gene names

Name:	L3mbtl3
Synonyms:	Mbt1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	883 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Putative Polycomb group (PcG) protein. PcG proteins maintain the transcriptionally repressive state of genes, probably via a modification of chromatin, rendering it heritably changed in its expressibility By similarity. Required for normal maturation of myeloid progenitor cells. Ref.4
Subunit structure	Interacts with RNF2. Ref.4
Subcellular location	Nucleus Ref.4.
Tissue specificity	Detected in hematopoietic progenitor cells in fetal liver. Detected in adult bone marrow, heart, brain, spleen, lung, liver, kidney and testis. Ref.4
Disruption phenotype	Death at a late embryonic stage due defective erythropoiesis, defects in the maturation of other types of myeloid lineage cells and anemia. Ref.4
Sequence similarities	Contains 3 MBT repeats. Contains 1 SAM (sterile alpha motif) domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat
Molecular function	Chromatin regulator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8BLB7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8BLB7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     72-96: Missing.

Isoform 3 (identifier: Q8BLB7-3)

The sequence of this isoform differs from the canonical sequence as follows:
     290-302: CGYRIKLHFDGYS → SSALYLFGKRDDG
     303-883: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 883	883	Lethal(3)malignant brain tumor-like protein 3		PRO_0000084451
Regions
	Repeat	232 – 332	101	MBT 1
	Repeat	340 – 439	100	MBT 2
	Repeat	448 – 543	96	MBT 3
	Domain	811 – 875	65	SAM
	Compositional bias	667 – 770	104	Gln/Pro-rich
Natural variations
	Alternative sequence	72 – 96	25	Missing in isoform 2.		VSP_013509
	Alternative sequence	290 – 302	13	CGYRI…FDGYS → SSALYLFGKRDDG in isoform 3.		VSP_013510
	Alternative sequence	303 – 883	581	Missing in isoform 3.		VSP_013511
Experimental info
	Sequence conflict	146	1	H → Y in BAC98253. Ref.3
	Sequence conflict	275	1	P → H in BAC27386. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 7A6417202A0E5532	FASTA	883	99,137
	10 20 30 40 50 60 MTESASSTSG QEFDVFSVMD WKDGVGTLPG SDLKFRVNEF GALEVITDES EMESVKKATA 70 80 90 100 110 120 TTTWMVPTAQ DAPTSPPSSR PVFPPAYWTS PPGCPTVFSE KTGVPFRLKE QSKADGLQFC 130 140 150 160 170 180 ENCCQYGNGD ECLSGGKYCS QNCARHAKDK DQKDERDGGE DNDEEDPKCS RKKKPKLSLK 190 200 210 220 230 240 ADSKDDGEER DDEMENKQDG RILRGSQRAR RKRRGDSAVL KQGLPPKGKK TWCWASYLEE 250 260 270 280 290 300 EKAVAVPTKL FKEHQSFPYN KNGFKVGMKL EGVDPDHQAM YCVLTVAEVC GYRIKLHFDG 310 320 330 340 350 360 YSDCYDFWVN ADALDIHPVG WCEKTGHKLR PPKGYKEEEF NWQSYLKTCK AQAAPKSLFE 370 380 390 400 410 420 NQNITVIPSG FRVGMKLEAA DKKSPSVICV ATVTDMVDNR FLVHFDNWDE SYDYWCESNS 430 440 450 460 470 480 PHIHPVGWCK EHRRTLITPP GYSHVKHFSW DKYLEETNSL PAPARAFKVK PPHGFQKKMK 490 500 510 520 530 540 LEAVDKRNPL FIRVATVADT DDHRIKVHFD GWSSCYDYWI DADSPDIHPV GWCSKTGHPL 550 560 570 580 590 600 QAPLSPAELM EPSETGGCPT LGCRGVGHFK KSRYLGTQSG ANCPYSEINL SKERIFPDRL 610 620 630 640 650 660 SGDTSPPTTP SFPRSKRMDT RESSSSPETR EKHANNFKED SEKKKENEVK TSAEAKVVRE 670 680 690 700 710 720 EPTPSVQQSQ PPQQVQQVQH AQPPQQAQKA PQAQQAQQAQ QAQQAPQAPQ TPQPQQAPQV 730 740 750 760 770 780 QQAQQAPQAQ QAQQPQQAQQ PQQAPPVQQP QQVQQAQPTQ QQAQTQQQAQ RRSAVFLSFK 790 800 810 820 830 840 PPIPCLPLRW EQQSKLLPTV AGIPASRVSK WSTDEVSEFI QSLPGCEEHG KVFKDEQIDG 850 860 870 880 EAFLLMTQTD IVKIMSIKLG PALKIFNSIL MFKAAEKNSH NEL
	Isoform 2 [UniParc]. Checksum: 1F35B839BCA63AC5	FASTA	858	96,557
	10 20 30 40 50 60 MTESASSTSG QEFDVFSVMD WKDGVGTLPG SDLKFRVNEF GALEVITDES EMESVKKATA 70 80 90 100 110 120 TTTWMVPTAQ DVFSEKTGVP FRLKEQSKAD GLQFCENCCQ YGNGDECLSG GKYCSQNCAR 130 140 150 160 170 180 HAKDKDQKDE RDGGEDNDEE DPKCSRKKKP KLSLKADSKD DGEERDDEME NKQDGRILRG 190 200 210 220 230 240 SQRARRKRRG DSAVLKQGLP PKGKKTWCWA SYLEEEKAVA VPTKLFKEHQ SFPYNKNGFK 250 260 270 280 290 300 VGMKLEGVDP DHQAMYCVLT VAEVCGYRIK LHFDGYSDCY DFWVNADALD IHPVGWCEKT 310 320 330 340 350 360 GHKLRPPKGY KEEEFNWQSY LKTCKAQAAP KSLFENQNIT VIPSGFRVGM KLEAADKKSP 370 380 390 400 410 420 SVICVATVTD MVDNRFLVHF DNWDESYDYW CESNSPHIHP VGWCKEHRRT LITPPGYSHV 430 440 450 460 470 480 KHFSWDKYLE ETNSLPAPAR AFKVKPPHGF QKKMKLEAVD KRNPLFIRVA TVADTDDHRI 490 500 510 520 530 540 KVHFDGWSSC YDYWIDADSP DIHPVGWCSK TGHPLQAPLS PAELMEPSET GGCPTLGCRG 550 560 570 580 590 600 VGHFKKSRYL GTQSGANCPY SEINLSKERI FPDRLSGDTS PPTTPSFPRS KRMDTRESSS 610 620 630 640 650 660 SPETREKHAN NFKEDSEKKK ENEVKTSAEA KVVREEPTPS VQQSQPPQQV QQVQHAQPPQ 670 680 690 700 710 720 QAQKAPQAQQ AQQAQQAQQA PQAPQTPQPQ QAPQVQQAQQ APQAQQAQQP QQAQQPQQAP 730 740 750 760 770 780 PVQQPQQVQQ AQPTQQQAQT QQQAQRRSAV FLSFKPPIPC LPLRWEQQSK LLPTVAGIPA 790 800 810 820 830 840 SRVSKWSTDE VSEFIQSLPG CEEHGKVFKD EQIDGEAFLL MTQTDIVKIM SIKLGPALKI 850 FNSILMFKAA EKNSHNEL
	Isoform 3 [UniParc]. Checksum: E6BB01D49BB19B4B	FASTA	302	33,556
	10 20 30 40 50 60 MTESASSTSG QEFDVFSVMD WKDGVGTLPG SDLKFRVNEF GALEVITDES EMESVKKATA 70 80 90 100 110 120 TTTWMVPTAQ DAPTSPPSSR PVFPPAYWTS PPGCPTVFSE KTGVPFRLKE QSKADGLQFC 130 140 150 160 170 180 ENCCQYGNGD ECLSGGKYCS QNCARHAKDK DQKDERDGGE DNDEEDPKCS RKKKPKLSLK 190 200 210 220 230 240 ADSKDDGEER DDEMENKQDG RILRGSQRAR RKRRGDSAVL KQGLPPKGKK TWCWASYLEE 250 260 270 280 290 300 EKAVAVPTKL FKEHQSFPYN KNGFKVGMKL EGVDPDHQAM YCVLTVAEVS SALYLFGKRD DG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Strain: C57BL/6J. Tissue: Corpora quadrigemina and Fetal testis.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6. Tissue: Brain and Eye.
[3]	"Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H. DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 123-883. Tissue: Embryonic tail.
[4]	"Impaired maturation of myeloid progenitors in mice lacking novel Polycomb group protein MBT-1." Arai S., Miyazaki T. EMBO J. 24:1863-1873(2005) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK031398 mRNA. Translation: BAC27386.1. AK045667 mRNA. Translation: BAC32449.1. BC082309 mRNA. Translation: AAH82309.1. BC085192 mRNA. Translation: AAH85192.1. AK129443 mRNA. Translation: BAC98253.1.
IPI	IPI00222779. IPI00556705. IPI00556736.
RefSeq	NP_766375.1. NM_172787.2.
UniGene	Mm.338439.
3D structure databases
ProteinModelPortal	Q8BLB7.
SMR	Q8BLB7. Positions 232-545, 808-865.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000037619.
PTM databases
PhosphoSite	Q8BLB7.
Proteomic databases
PRIDE	Q8BLB7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000040219; ENSMUSP00000037619; ENSMUSG00000039089. ENSMUST00000105519; ENSMUSP00000101158; ENSMUSG00000039089. ENSMUST00000174766; ENSMUSP00000133479; ENSMUSG00000039089.
GeneID	237339.
KEGG	mmu:237339.
UCSC	uc007esa.1. mouse. uc007esb.1. mouse. uc007esd.1. mouse.
Organism-specific databases
CTD	84456.
MGI	MGI:2143628. L3mbtl3.
Phylogenomic databases
eggNOG	NOG281100.
GeneTree	ENSGT00560000076796.
HOGENOM	HOG000231019.
HOVERGEN	HBG071375.
InParanoid	Q8BLB7.
OMA	EYQSFPY.
OrthoDB	EOG41VK2D.
Gene expression databases
ArrayExpress	Q8BLB7.
Bgee	Q8BLB7.
CleanEx	MM_L3MBTL3.
Genevestigator	Q8BLB7.
GermOnline	ENSMUSG00000039089. Mus musculus.
Family and domain databases
Gene3D	1.10.150.50. 1 hit.
InterPro	IPR004092. Mbt. IPR001660. SAM. IPR013761. SAM/pointed. IPR021129. SAM_type1. [Graphical view]
Pfam	PF02820. MBT. 3 hits. PF00536. SAM_1. 1 hit. [Graphical view]
SMART	SM00561. MBT. 3 hits. SM00454. SAM. 1 hit. [Graphical view]
SUPFAM	SSF47769. SAM_homology. 1 hit.
PROSITE	PS51079. MBT. 3 hits. PS50105. SAM_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	383306.
SOURCE	Search...

Entry information

Entry name

LMBL3_MOUSE

Accession

Primary (citable) accession number: Q8BLB7
Secondary accession number(s): Q0VGT0 , Q641L7, Q6ZPI2, Q8C0G4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program