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Q8BLA8

- TRPA1_MOUSE

UniProt

Q8BLA8 - TRPA1_MOUSE

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Protein

Transient receptor potential cation channel subfamily A member 1

Gene
Trpa1, Anktm1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Receptor-activated non-selective cation channel involved in detection of pain and possibly also in cold perception and inner ear function. Has a central role in the pain response to endogenous inflammatory mediators and to a diverse array of volatile irritants, such as mustard oil, garlic and acrolein, an irritant from tears gas and vehicule exhaust fumes. Acts also as a ionotropic cannabinoid receptor by being activated by delta(9)-tetrahydrocannabinol (THC), the psychoactive component of marijuana. Not involved in menthol sensation. May be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. Probably operated by a phosphatidylinositol second messenger system.4 Publications

Enzyme regulationi

Inhibited by ruthenium red, a potent blocker of TRPV channels.

GO - Molecular functioni

  1. calcium channel activity Source: MGI
  2. channel activity Source: MGI
  3. ion channel activity Source: MGI

GO - Biological processi

  1. calcium ion transmembrane transport Source: GOC
  2. calcium ion transport Source: MGI
  3. detection of chemical stimulus involved in sensory perception of pain Source: MGI
  4. detection of mechanical stimulus involved in sensory perception of pain Source: MGI
  5. ion transmembrane transport Source: GOC
  6. response to cold Source: MGI
  7. response to drug Source: MGI
  8. response to hydrogen peroxide Source: MGI
  9. response to pain Source: MGI
  10. thermoception Source: MGI
  11. transmembrane transport Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Ion channel

Keywords - Biological processi

Ion transport, Sensory transduction, Transport

Enzyme and pathway databases

ReactomeiREACT_227861. TRP channels.

Protein family/group databases

TCDBi1.A.4.6.1. the transient receptor potential ca(2+) channel (trp-cc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Transient receptor potential cation channel subfamily A member 1
Alternative name(s):
Ankyrin-like with transmembrane domains protein 1
Gene namesi
Name:Trpa1
Synonyms:Anktm1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:3522699. Trpa1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 719719Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei720 – 74021Helical; Reviewed predictionAdd
BLAST
Topological domaini741 – 76727Extracellular Reviewed predictionAdd
BLAST
Transmembranei768 – 78821Helical; Reviewed predictionAdd
BLAST
Topological domaini789 – 81123Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei812 – 83221Helical; Reviewed predictionAdd
BLAST
Transmembranei833 – 85321Helical; Reviewed predictionAdd
BLAST
Topological domaini854 – 87623Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei877 – 89721Helical; Reviewed predictionAdd
BLAST
Topological domaini898 – 94346Extracellular Reviewed predictionAdd
BLAST
Transmembranei944 – 96421Helical; Reviewed predictionAdd
BLAST
Topological domaini965 – 1125161Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: MGI
  2. stereocilium bundle Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Mice display normal cold sensitivity and unimpaired auditory function, suggesting that this channel is not required for the initial detection of noxious cold or sound. However, they exhibit pronounced deficits in bradykinin-evoked nociceptor excitation and pain hypersensitivity.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11251125Transient receptor potential cation channel subfamily A member 1PRO_0000215370Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi193 ↔ 666Alternate1 Publication
Disulfide bondi463 ↔ 666Alternate1 Publication
Disulfide bondi609 ↔ 622Alternate1 Publication
Disulfide bondi622 ↔ 666Alternate1 Publication
Glycosylationi749 – 7491N-linked (GlcNAc...) Reviewed prediction
Glycosylationi755 – 7551N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

TRPA1 activation by electrophiles occurs though covalent modification of specific cysteine residues in the N-terminal cytoplasmic domain.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ8BLA8.

PTM databases

PhosphoSiteiQ8BLA8.

Expressioni

Tissue specificityi

Expressed in inner ear (at protein level). Specifically expressed in a subset of nociceptive neurons. Expressed in the same neurons that TRPV1. In contrast, it is not expressed in neurons expressing TRPM8. Expressed in the superior cervical ganglion.4 Publications

Developmental stagei

During utricle development, it is first expressed at E15 and E16 and peaks at E17. It drops at E18 but increases again at E19, possibly corresponding to a second wave of hair cells that are generated at E15.1 Publication

Gene expression databases

BgeeiQ8BLA8.
GenevestigatoriQ8BLA8.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ8BLA8.
SMRiQ8BLA8. Positions 30-640, 793-973.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati63 – 9432ANK 1Add
BLAST
Repeati98 – 12730ANK 2Add
BLAST
Repeati131 – 16131ANK 3Add
BLAST
Repeati165 – 19430ANK 4Add
BLAST
Repeati198 – 22730ANK 5Add
BLAST
Repeati239 – 26830ANK 6Add
BLAST
Repeati272 – 30130ANK 7Add
BLAST
Repeati309 – 33830ANK 8Add
BLAST
Repeati342 – 37130ANK 9Add
BLAST
Repeati413 – 44230ANK 10Add
BLAST
Repeati446 – 47530ANK 11Add
BLAST
Repeati482 – 51130ANK 12Add
BLAST
Repeati514 – 54330ANK 13Add
BLAST
Repeati548 – 57730ANK 14Add
BLAST

Sequence similaritiesi

Contains 14 ANK repeats.

Keywords - Domaini

ANK repeat, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0666.
GeneTreeiENSGT00740000115514.
HOGENOMiHOG000044486.
HOVERGENiHBG059027.
InParanoidiQ8BLA8.
KOiK04984.
OMAiWLAYGFR.
OrthoDBiEOG7P02H2.
PhylomeDBiQ8BLA8.
TreeFamiTF317264.

Family and domain databases

Gene3Di1.25.40.20. 4 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
PfamiPF12796. Ank_2. 6 hits.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 14 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 2 hits.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 9 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BLA8-1 [UniParc]FASTAAdd to Basket

« Hide

MKRGLRRILL PEERKEVQGV VYRGVGEDMD CSKESFKVDI EGDMCRLEDF     50
IKNRRKLSKY EDENLCPLHH AAAEGQVELM ELIINGSSCE VLNIMDGYGN 100
TPLHCAAEKN QVESVKFLLS QGANPNLRNR NMMSPLHIAV HGMYNEVIKV 150
LTEHKATNIN LEGENGNTAL MSTCAKDNSE ALQILLEKGA KLCKSNKWGD 200
YPVHQAAFSG AKKCMELILA YGEKNGYSRE THINFVNHKK ASPLHLAVQS 250
GDLDMIKMCL DNGAHIDMME NAKCMALHFA ATQGATDIVK LMISSYTGSS 300
DIVNAVDGNQ ETLLHRASLF DHHDLAEYLI SVGADINSTD SEGRSPLILA 350
TASASWNIVN LLLCKGAKVD IKDHLGRNFL HLTVQQPYGL RNLRPEFMQM 400
QHIKELVMDE DNDGCTPLHY ACRQGVPVSV NNLLGFNVSI HSKSKDKKSP 450
LHFAASYGRI NTCQRLLQDI SDTRLLNEGD LHGMTPLHLA AKNGHDKVVQ 500
LLLKKGALFL SDHNGWTALH HASMGGYTQT MKVILDTNLK CTDRLDEEGN 550
TALHFAAREG HAKAVAMLLS YNADILLNKK QASFLHIALH NKRKEVVLTT 600
IRNKRWDECL QVFTHNSPSN RCPIMEMVEY LPECMKVLLD FCMIPSTEDK 650
SCQDYHIEYN FKYLQCPLSM TKKVAPTQDV VYEPLTILNV MVQHNRIELL 700
NHPVCREYLL MKWCAYGFRA HMMNLGSYCL GLIPMTLLVV KIQPGMAFNS 750
TGIINGTSST HEERIDTLNS FPIKICMILV FLSSIFGYCK EVIQIFQQKR 800
NYFLDYNNAL EWVIYTTSII FVLPLFLNIP AYMQWQCGAI AIFFYWMNFL 850
LYLQRFENCG IFIVMLEVIF KTLLRSTGVF IFLLLAFGLS FYVLLNFQDA 900
FSTPLLSLIQ TFSMMLGDIN YRDAFLEPLF RNELAYPVLT FGQLIAFTMF 950
VPIVLMNLLI GLAVGDIAEV QKHASLKRIA MQVELHTNLE KKLPLWYLRK 1000
VDQRSTIVYP NRPRHGRMLR FFHYFLNMQE TRQEVPNIDT CLEMEILKQK 1050
YRLKDLTSLL EKQHELIKLI IQKMEIISET EDEDNHCSFQ DRFKKERLEQ 1100
MHSKWNFVLN AVKTKTHCSI SHPDF 1125
Length:1,125
Mass (Da):128,467
Last modified:March 1, 2003 - v1
Checksum:iCD718BF3ED4AC291
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY231177 mRNA. Translation: AAO43183.1.
AK045771 mRNA. Translation: BAC32487.1.
BC120563 mRNA. Translation: AAI20564.1.
BC131963 mRNA. Translation: AAI31964.1.
CCDSiCCDS35516.1.
RefSeqiNP_808449.1. NM_177781.4.
XP_006495588.1. XM_006495525.1.
UniGeneiMm.186329.

Genome annotation databases

EnsembliENSMUST00000041447; ENSMUSP00000043594; ENSMUSG00000032769.
GeneIDi277328.
KEGGimmu:277328.
UCSCiuc007ajc.1. mouse.

Cross-referencesi

Web resourcesi

Protein Spotlight

The power behind pain - Issue 82 of May 2007

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY231177 mRNA. Translation: AAO43183.1 .
AK045771 mRNA. Translation: BAC32487.1 .
BC120563 mRNA. Translation: AAI20564.1 .
BC131963 mRNA. Translation: AAI31964.1 .
CCDSi CCDS35516.1.
RefSeqi NP_808449.1. NM_177781.4.
XP_006495588.1. XM_006495525.1.
UniGenei Mm.186329.

3D structure databases

ProteinModelPortali Q8BLA8.
SMRi Q8BLA8. Positions 30-640, 793-973.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi Q8BLA8.
ChEMBLi CHEMBL1075310.
GuidetoPHARMACOLOGYi 485.

Protein family/group databases

TCDBi 1.A.4.6.1. the transient receptor potential ca(2+) channel (trp-cc) family.

PTM databases

PhosphoSitei Q8BLA8.

Proteomic databases

PRIDEi Q8BLA8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000041447 ; ENSMUSP00000043594 ; ENSMUSG00000032769 .
GeneIDi 277328.
KEGGi mmu:277328.
UCSCi uc007ajc.1. mouse.

Organism-specific databases

CTDi 8989.
MGIi MGI:3522699. Trpa1.

Phylogenomic databases

eggNOGi COG0666.
GeneTreei ENSGT00740000115514.
HOGENOMi HOG000044486.
HOVERGENi HBG059027.
InParanoidi Q8BLA8.
KOi K04984.
OMAi WLAYGFR.
OrthoDBi EOG7P02H2.
PhylomeDBi Q8BLA8.
TreeFami TF317264.

Enzyme and pathway databases

Reactomei REACT_227861. TRP channels.

Miscellaneous databases

NextBioi 393737.
PROi Q8BLA8.
SOURCEi Search...

Gene expression databases

Bgeei Q8BLA8.
Genevestigatori Q8BLA8.

Family and domain databases

Gene3Di 1.25.40.20. 4 hits.
InterProi IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view ]
Pfami PF12796. Ank_2. 6 hits.
[Graphical view ]
PRINTSi PR01415. ANKYRIN.
SMARTi SM00248. ANK. 14 hits.
[Graphical view ]
SUPFAMi SSF48403. SSF48403. 2 hits.
PROSITEi PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 9 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "ANKTM1, a TRP-like channel expressed in nociceptive neurons, is activated by cold temperatures."
    Story G.M., Peier A.M., Reeve A.J., Eid S.R., Mosbacher J., Hricik T.R., Earley T.J., Hergarden A.C., Anderson D.A., Hwang S.W., McIntyre P., Jegla T., Bevan S., Patapoutian A.
    Cell 112:819-829(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Strain: C57BL/6.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Corpora quadrigemina.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  5. "Cold-sensitive, menthol-insensitive neurons in the murine sympathetic nervous system."
    Smith M.P., Beacham D., Ensor E., Koltzenburg M.
    NeuroReport 15:1399-1403(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. "Noxious cold ion channel TRPA1 is activated by pungent compounds and bradykinin."
    Bandell M., Story G.M., Hwang S.W., Viswanath V., Eid S.R., Petrus M.J., Earley T.J., Patapoutian A.
    Neuron 41:849-857(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Nociceptor and hair cell transducer properties of TRPA1, a channel for pain and hearing."
    Nagata K., Duggan A., Kumar G., Garcia-Anoveros J.
    J. Neurosci. 25:4052-4061(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  8. "TRPA1 mediates the inflammatory actions of environmental irritants and proalgesic agents."
    Bautista D.M., Jordt S.E., Nikai T., Tsuruda P.R., Read A.J., Poblete J., Yamoah E.N., Basbaum A.I., Julius D.
    Cell 124:1269-1282(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  9. "Identification of in vivo disulfide conformation of TRPA1 ion channel."
    Wang L., Cvetkov T.L., Chance M.R., Moiseenkova-Bell V.Y.
    J. Biol. Chem. 287:6169-6176(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISULFIDE BONDS.
  10. "Molecular architecture and subunit organization of TRPA1 ion channel revealed by electron microscopy."
    Cvetkov T.L., Huynh K.W., Cohen M.R., Moiseenkova-Bell V.Y.
    J. Biol. Chem. 286:38168-38176(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: ELECTRON MICROSCOPY (16 ANGSTROMS), SUBUNIT.

Entry informationi

Entry nameiTRPA1_MOUSE
AccessioniPrimary (citable) accession number: Q8BLA8
Secondary accession number(s): Q0VBN5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: March 1, 2003
Last modified: September 3, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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