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Q8BLA8

- TRPA1_MOUSE

UniProt

Q8BLA8 - TRPA1_MOUSE

Protein

Transient receptor potential cation channel subfamily A member 1

Gene

Trpa1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Receptor-activated non-selective cation channel involved in detection of pain and possibly also in cold perception and inner ear function. Has a central role in the pain response to endogenous inflammatory mediators and to a diverse array of volatile irritants, such as mustard oil, garlic and acrolein, an irritant from tears gas and vehicule exhaust fumes. Acts also as a ionotropic cannabinoid receptor by being activated by delta(9)-tetrahydrocannabinol (THC), the psychoactive component of marijuana. Not involved in menthol sensation. May be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. Probably operated by a phosphatidylinositol second messenger system.4 Publications

    Enzyme regulationi

    Inhibited by ruthenium red, a potent blocker of TRPV channels.

    GO - Molecular functioni

    1. calcium channel activity Source: MGI
    2. channel activity Source: MGI
    3. ion channel activity Source: MGI

    GO - Biological processi

    1. calcium ion transmembrane transport Source: GOC
    2. calcium ion transport Source: MGI
    3. detection of chemical stimulus involved in sensory perception of pain Source: MGI
    4. detection of mechanical stimulus involved in sensory perception of pain Source: MGI
    5. ion transmembrane transport Source: GOC
    6. response to cold Source: MGI
    7. response to drug Source: MGI
    8. response to hydrogen peroxide Source: MGI
    9. response to pain Source: MGI
    10. thermoception Source: MGI
    11. transmembrane transport Source: GOC

    Keywords - Molecular functioni

    Ion channel

    Keywords - Biological processi

    Ion transport, Sensory transduction, Transport

    Enzyme and pathway databases

    ReactomeiREACT_227861. TRP channels.

    Protein family/group databases

    TCDBi1.A.4.6.1. the transient receptor potential ca(2+) channel (trp-cc) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transient receptor potential cation channel subfamily A member 1
    Alternative name(s):
    Ankyrin-like with transmembrane domains protein 1
    Gene namesi
    Name:Trpa1
    Synonyms:Anktm1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:3522699. Trpa1.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: MGI
    2. stereocilium bundle Source: MGI

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Mice display normal cold sensitivity and unimpaired auditory function, suggesting that this channel is not required for the initial detection of noxious cold or sound. However, they exhibit pronounced deficits in bradykinin-evoked nociceptor excitation and pain hypersensitivity.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11251125Transient receptor potential cation channel subfamily A member 1PRO_0000215370Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi193 ↔ 666Alternate1 Publication
    Disulfide bondi463 ↔ 666Alternate1 Publication
    Disulfide bondi609 ↔ 622Alternate1 Publication
    Disulfide bondi622 ↔ 666Alternate1 Publication
    Glycosylationi749 – 7491N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi755 – 7551N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    TRPA1 activation by electrophiles occurs though covalent modification of specific cysteine residues in the N-terminal cytoplasmic domain.

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiQ8BLA8.

    PTM databases

    PhosphoSiteiQ8BLA8.

    Expressioni

    Tissue specificityi

    Expressed in inner ear (at protein level). Specifically expressed in a subset of nociceptive neurons. Expressed in the same neurons that TRPV1. In contrast, it is not expressed in neurons expressing TRPM8. Expressed in the superior cervical ganglion.4 Publications

    Developmental stagei

    During utricle development, it is first expressed at E15 and E16 and peaks at E17. It drops at E18 but increases again at E19, possibly corresponding to a second wave of hair cells that are generated at E15.1 Publication

    Gene expression databases

    BgeeiQ8BLA8.
    GenevestigatoriQ8BLA8.

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BLA8.
    SMRiQ8BLA8. Positions 30-640, 793-973.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 719719CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini741 – 76727ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini789 – 81123CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini854 – 87623CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini898 – 94346ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini965 – 1125161CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei720 – 74021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei768 – 78821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei812 – 83221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei833 – 85321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei877 – 89721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei944 – 96421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati63 – 9432ANK 1Add
    BLAST
    Repeati98 – 12730ANK 2Add
    BLAST
    Repeati131 – 16131ANK 3Add
    BLAST
    Repeati165 – 19430ANK 4Add
    BLAST
    Repeati198 – 22730ANK 5Add
    BLAST
    Repeati239 – 26830ANK 6Add
    BLAST
    Repeati272 – 30130ANK 7Add
    BLAST
    Repeati309 – 33830ANK 8Add
    BLAST
    Repeati342 – 37130ANK 9Add
    BLAST
    Repeati413 – 44230ANK 10Add
    BLAST
    Repeati446 – 47530ANK 11Add
    BLAST
    Repeati482 – 51130ANK 12Add
    BLAST
    Repeati514 – 54330ANK 13Add
    BLAST
    Repeati548 – 57730ANK 14Add
    BLAST

    Sequence similaritiesi

    Contains 14 ANK repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    ANK repeat, Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0666.
    GeneTreeiENSGT00740000115514.
    HOGENOMiHOG000044486.
    HOVERGENiHBG059027.
    InParanoidiQ8BLA8.
    KOiK04984.
    OMAiWLAYGFR.
    OrthoDBiEOG7P02H2.
    PhylomeDBiQ8BLA8.
    TreeFamiTF317264.

    Family and domain databases

    Gene3Di1.25.40.20. 4 hits.
    InterProiIPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    [Graphical view]
    PfamiPF12796. Ank_2. 6 hits.
    [Graphical view]
    PRINTSiPR01415. ANKYRIN.
    SMARTiSM00248. ANK. 14 hits.
    [Graphical view]
    SUPFAMiSSF48403. SSF48403. 2 hits.
    PROSITEiPS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 9 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8BLA8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKRGLRRILL PEERKEVQGV VYRGVGEDMD CSKESFKVDI EGDMCRLEDF     50
    IKNRRKLSKY EDENLCPLHH AAAEGQVELM ELIINGSSCE VLNIMDGYGN 100
    TPLHCAAEKN QVESVKFLLS QGANPNLRNR NMMSPLHIAV HGMYNEVIKV 150
    LTEHKATNIN LEGENGNTAL MSTCAKDNSE ALQILLEKGA KLCKSNKWGD 200
    YPVHQAAFSG AKKCMELILA YGEKNGYSRE THINFVNHKK ASPLHLAVQS 250
    GDLDMIKMCL DNGAHIDMME NAKCMALHFA ATQGATDIVK LMISSYTGSS 300
    DIVNAVDGNQ ETLLHRASLF DHHDLAEYLI SVGADINSTD SEGRSPLILA 350
    TASASWNIVN LLLCKGAKVD IKDHLGRNFL HLTVQQPYGL RNLRPEFMQM 400
    QHIKELVMDE DNDGCTPLHY ACRQGVPVSV NNLLGFNVSI HSKSKDKKSP 450
    LHFAASYGRI NTCQRLLQDI SDTRLLNEGD LHGMTPLHLA AKNGHDKVVQ 500
    LLLKKGALFL SDHNGWTALH HASMGGYTQT MKVILDTNLK CTDRLDEEGN 550
    TALHFAAREG HAKAVAMLLS YNADILLNKK QASFLHIALH NKRKEVVLTT 600
    IRNKRWDECL QVFTHNSPSN RCPIMEMVEY LPECMKVLLD FCMIPSTEDK 650
    SCQDYHIEYN FKYLQCPLSM TKKVAPTQDV VYEPLTILNV MVQHNRIELL 700
    NHPVCREYLL MKWCAYGFRA HMMNLGSYCL GLIPMTLLVV KIQPGMAFNS 750
    TGIINGTSST HEERIDTLNS FPIKICMILV FLSSIFGYCK EVIQIFQQKR 800
    NYFLDYNNAL EWVIYTTSII FVLPLFLNIP AYMQWQCGAI AIFFYWMNFL 850
    LYLQRFENCG IFIVMLEVIF KTLLRSTGVF IFLLLAFGLS FYVLLNFQDA 900
    FSTPLLSLIQ TFSMMLGDIN YRDAFLEPLF RNELAYPVLT FGQLIAFTMF 950
    VPIVLMNLLI GLAVGDIAEV QKHASLKRIA MQVELHTNLE KKLPLWYLRK 1000
    VDQRSTIVYP NRPRHGRMLR FFHYFLNMQE TRQEVPNIDT CLEMEILKQK 1050
    YRLKDLTSLL EKQHELIKLI IQKMEIISET EDEDNHCSFQ DRFKKERLEQ 1100
    MHSKWNFVLN AVKTKTHCSI SHPDF 1125
    Length:1,125
    Mass (Da):128,467
    Last modified:March 1, 2003 - v1
    Checksum:iCD718BF3ED4AC291
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY231177 mRNA. Translation: AAO43183.1.
    AK045771 mRNA. Translation: BAC32487.1.
    BC120563 mRNA. Translation: AAI20564.1.
    BC131963 mRNA. Translation: AAI31964.1.
    CCDSiCCDS35516.1.
    RefSeqiNP_808449.1. NM_177781.4.
    XP_006495588.1. XM_006495525.1.
    UniGeneiMm.186329.

    Genome annotation databases

    EnsembliENSMUST00000041447; ENSMUSP00000043594; ENSMUSG00000032769.
    GeneIDi277328.
    KEGGimmu:277328.
    UCSCiuc007ajc.1. mouse.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    The power behind pain - Issue 82 of May 2007

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY231177 mRNA. Translation: AAO43183.1 .
    AK045771 mRNA. Translation: BAC32487.1 .
    BC120563 mRNA. Translation: AAI20564.1 .
    BC131963 mRNA. Translation: AAI31964.1 .
    CCDSi CCDS35516.1.
    RefSeqi NP_808449.1. NM_177781.4.
    XP_006495588.1. XM_006495525.1.
    UniGenei Mm.186329.

    3D structure databases

    ProteinModelPortali Q8BLA8.
    SMRi Q8BLA8. Positions 30-640, 793-973.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi Q8BLA8.
    ChEMBLi CHEMBL1075310.
    GuidetoPHARMACOLOGYi 485.

    Protein family/group databases

    TCDBi 1.A.4.6.1. the transient receptor potential ca(2+) channel (trp-cc) family.

    PTM databases

    PhosphoSitei Q8BLA8.

    Proteomic databases

    PRIDEi Q8BLA8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000041447 ; ENSMUSP00000043594 ; ENSMUSG00000032769 .
    GeneIDi 277328.
    KEGGi mmu:277328.
    UCSCi uc007ajc.1. mouse.

    Organism-specific databases

    CTDi 8989.
    MGIi MGI:3522699. Trpa1.

    Phylogenomic databases

    eggNOGi COG0666.
    GeneTreei ENSGT00740000115514.
    HOGENOMi HOG000044486.
    HOVERGENi HBG059027.
    InParanoidi Q8BLA8.
    KOi K04984.
    OMAi WLAYGFR.
    OrthoDBi EOG7P02H2.
    PhylomeDBi Q8BLA8.
    TreeFami TF317264.

    Enzyme and pathway databases

    Reactomei REACT_227861. TRP channels.

    Miscellaneous databases

    NextBioi 393737.
    PROi Q8BLA8.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8BLA8.
    Genevestigatori Q8BLA8.

    Family and domain databases

    Gene3Di 1.25.40.20. 4 hits.
    InterProi IPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    [Graphical view ]
    Pfami PF12796. Ank_2. 6 hits.
    [Graphical view ]
    PRINTSi PR01415. ANKYRIN.
    SMARTi SM00248. ANK. 14 hits.
    [Graphical view ]
    SUPFAMi SSF48403. SSF48403. 2 hits.
    PROSITEi PS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 9 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "ANKTM1, a TRP-like channel expressed in nociceptive neurons, is activated by cold temperatures."
      Story G.M., Peier A.M., Reeve A.J., Eid S.R., Mosbacher J., Hricik T.R., Earley T.J., Hergarden A.C., Anderson D.A., Hwang S.W., McIntyre P., Jegla T., Bevan S., Patapoutian A.
      Cell 112:819-829(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
      Strain: C57BL/6.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Corpora quadrigemina.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    4. Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    5. "Cold-sensitive, menthol-insensitive neurons in the murine sympathetic nervous system."
      Smith M.P., Beacham D., Ensor E., Koltzenburg M.
      NeuroReport 15:1399-1403(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    6. "Noxious cold ion channel TRPA1 is activated by pungent compounds and bradykinin."
      Bandell M., Story G.M., Hwang S.W., Viswanath V., Eid S.R., Petrus M.J., Earley T.J., Patapoutian A.
      Neuron 41:849-857(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Nociceptor and hair cell transducer properties of TRPA1, a channel for pain and hearing."
      Nagata K., Duggan A., Kumar G., Garcia-Anoveros J.
      J. Neurosci. 25:4052-4061(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.
    8. "TRPA1 mediates the inflammatory actions of environmental irritants and proalgesic agents."
      Bautista D.M., Jordt S.E., Nikai T., Tsuruda P.R., Read A.J., Poblete J., Yamoah E.N., Basbaum A.I., Julius D.
      Cell 124:1269-1282(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
    9. "Identification of in vivo disulfide conformation of TRPA1 ion channel."
      Wang L., Cvetkov T.L., Chance M.R., Moiseenkova-Bell V.Y.
      J. Biol. Chem. 287:6169-6176(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISULFIDE BONDS.
    10. "Molecular architecture and subunit organization of TRPA1 ion channel revealed by electron microscopy."
      Cvetkov T.L., Huynh K.W., Cohen M.R., Moiseenkova-Bell V.Y.
      J. Biol. Chem. 286:38168-38176(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: ELECTRON MICROSCOPY (16 ANGSTROMS), SUBUNIT.

    Entry informationi

    Entry nameiTRPA1_MOUSE
    AccessioniPrimary (citable) accession number: Q8BLA8
    Secondary accession number(s): Q0VBN5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 21, 2004
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3