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Q8BLA8 (TRPA1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transient receptor potential cation channel subfamily A member 1
Alternative name(s):
Ankyrin-like with transmembrane domains protein 1
Gene names
Name:Trpa1
Synonyms:Anktm1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1125 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor-activated non-selective cation channel involved in detection of pain and possibly also in cold perception and inner ear function. Has a central role in the pain response to endogenous inflammatory mediators and to a diverse array of volatile irritants, such as mustard oil, garlic and acrolein, an irritant from tears gas and vehicule exhaust fumes. Acts also as a ionotropic cannabinoid receptor by being activated by delta(9)-tetrahydrocannabinol (THC), the psychoactive component of marijuana. Not involved in menthol sensation. May be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. Probably operated by a phosphatidylinositol second messenger system. Ref.1 Ref.4 Ref.6 Ref.7

Enzyme regulation

Inhibited by ruthenium red, a potent blocker of TRPV channels.

Subunit structure

Homotetramer. Ref.10

Subcellular location

Membrane; Multi-pass membrane protein Probable.

Tissue specificity

Expressed in inner ear (at protein level). Specifically expressed in a subset of nociceptive neurons. Expressed in the same neurons that TRPV1. In contrast, it is not expressed in neurons expressing TRPM8. Expressed in the superior cervical ganglion. Ref.1 Ref.4 Ref.5 Ref.7

Developmental stage

During utricle development, it is first expressed at E15 and E16 and peaks at E17. It drops at E18 but increases again at E19, possibly corresponding to a second wave of hair cells that are generated at E15. Ref.4

Post-translational modification

TRPA1 activation by electrophiles occurs though covalent modification of specific cysteine residues in the N-terminal cytoplasmic domain.

Disruption phenotype

Mice display normal cold sensitivity and unimpaired auditory function, suggesting that this channel is not required for the initial detection of noxious cold or sound. However, they exhibit pronounced deficits in bradykinin-evoked nociceptor excitation and pain hypersensitivity. Ref.8

Sequence similarities

Belongs to the transient receptor (TC 1.A.4) family. [View classification]

Contains 14 ANK repeats.

Ontologies

Keywords
   Biological processIon transport
Sensory transduction
Transport
   Cellular componentMembrane
   DomainANK repeat
Repeat
Transmembrane
Transmembrane helix
   Molecular functionIon channel
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcalcium ion transmembrane transport

Inferred from direct assay Ref.1PubMed 17588549. Source: GOC

calcium ion transport

Inferred from direct assay PubMed 17588549. Source: MGI

detection of chemical stimulus involved in sensory perception of pain

Inferred from direct assay PubMed 18272293. Source: MGI

detection of mechanical stimulus involved in sensory perception of pain

Inferred from mutant phenotype PubMed 16630838. Source: MGI

ion transmembrane transport

Inferred from direct assay Ref.7. Source: GOC

response to cold

Inferred from direct assay Ref.1. Source: MGI

response to drug

Inferred from direct assay PubMed 18272293. Source: MGI

response to hydrogen peroxide

Inferred from mutant phenotype PubMed 18398506. Source: MGI

response to pain

Inferred from direct assay PubMed 18272293. Source: MGI

thermoception

Inferred from direct assay Ref.1. Source: MGI

transmembrane transport

Inferred from direct assay Ref.4. Source: GOC

   Cellular_componentintegral component of membrane

Inferred from sequence or structural similarity Ref.1. Source: MGI

stereocilium bundle

Inferred from direct assay Ref.4Ref.7. Source: MGI

   Molecular_functioncalcium channel activity

Inferred from direct assay Ref.1PubMed 17588549. Source: MGI

channel activity

Inferred from direct assay Ref.4. Source: MGI

ion channel activity

Inferred from direct assay Ref.7. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11251125Transient receptor potential cation channel subfamily A member 1
PRO_0000215370

Regions

Topological domain1 – 719719Cytoplasmic Potential
Transmembrane720 – 74021Helical; Potential
Topological domain741 – 76727Extracellular Potential
Transmembrane768 – 78821Helical; Potential
Topological domain789 – 81123Cytoplasmic Potential
Transmembrane812 – 83221Helical; Potential
Transmembrane833 – 85321Helical; Potential
Topological domain854 – 87623Cytoplasmic Potential
Transmembrane877 – 89721Helical; Potential
Topological domain898 – 94346Extracellular Potential
Transmembrane944 – 96421Helical; Potential
Topological domain965 – 1125161Cytoplasmic Potential
Repeat63 – 9432ANK 1
Repeat98 – 12730ANK 2
Repeat131 – 16131ANK 3
Repeat165 – 19430ANK 4
Repeat198 – 22730ANK 5
Repeat239 – 26830ANK 6
Repeat272 – 30130ANK 7
Repeat309 – 33830ANK 8
Repeat342 – 37130ANK 9
Repeat413 – 44230ANK 10
Repeat446 – 47530ANK 11
Repeat482 – 51130ANK 12
Repeat514 – 54330ANK 13
Repeat548 – 57730ANK 14

Amino acid modifications

Glycosylation7491N-linked (GlcNAc...) Potential
Glycosylation7551N-linked (GlcNAc...) Potential
Disulfide bond193 ↔ 666Alternate Ref.9
Disulfide bond463 ↔ 666Alternate Ref.9
Disulfide bond609 ↔ 622Alternate Ref.9
Disulfide bond622 ↔ 666Alternate Ref.9

Sequences

Sequence LengthMass (Da)Tools
Q8BLA8 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: CD718BF3ED4AC291

FASTA1,125128,467
        10         20         30         40         50         60 
MKRGLRRILL PEERKEVQGV VYRGVGEDMD CSKESFKVDI EGDMCRLEDF IKNRRKLSKY 

        70         80         90        100        110        120 
EDENLCPLHH AAAEGQVELM ELIINGSSCE VLNIMDGYGN TPLHCAAEKN QVESVKFLLS 

       130        140        150        160        170        180 
QGANPNLRNR NMMSPLHIAV HGMYNEVIKV LTEHKATNIN LEGENGNTAL MSTCAKDNSE 

       190        200        210        220        230        240 
ALQILLEKGA KLCKSNKWGD YPVHQAAFSG AKKCMELILA YGEKNGYSRE THINFVNHKK 

       250        260        270        280        290        300 
ASPLHLAVQS GDLDMIKMCL DNGAHIDMME NAKCMALHFA ATQGATDIVK LMISSYTGSS 

       310        320        330        340        350        360 
DIVNAVDGNQ ETLLHRASLF DHHDLAEYLI SVGADINSTD SEGRSPLILA TASASWNIVN 

       370        380        390        400        410        420 
LLLCKGAKVD IKDHLGRNFL HLTVQQPYGL RNLRPEFMQM QHIKELVMDE DNDGCTPLHY 

       430        440        450        460        470        480 
ACRQGVPVSV NNLLGFNVSI HSKSKDKKSP LHFAASYGRI NTCQRLLQDI SDTRLLNEGD 

       490        500        510        520        530        540 
LHGMTPLHLA AKNGHDKVVQ LLLKKGALFL SDHNGWTALH HASMGGYTQT MKVILDTNLK 

       550        560        570        580        590        600 
CTDRLDEEGN TALHFAAREG HAKAVAMLLS YNADILLNKK QASFLHIALH NKRKEVVLTT 

       610        620        630        640        650        660 
IRNKRWDECL QVFTHNSPSN RCPIMEMVEY LPECMKVLLD FCMIPSTEDK SCQDYHIEYN 

       670        680        690        700        710        720 
FKYLQCPLSM TKKVAPTQDV VYEPLTILNV MVQHNRIELL NHPVCREYLL MKWCAYGFRA 

       730        740        750        760        770        780 
HMMNLGSYCL GLIPMTLLVV KIQPGMAFNS TGIINGTSST HEERIDTLNS FPIKICMILV 

       790        800        810        820        830        840 
FLSSIFGYCK EVIQIFQQKR NYFLDYNNAL EWVIYTTSII FVLPLFLNIP AYMQWQCGAI 

       850        860        870        880        890        900 
AIFFYWMNFL LYLQRFENCG IFIVMLEVIF KTLLRSTGVF IFLLLAFGLS FYVLLNFQDA 

       910        920        930        940        950        960 
FSTPLLSLIQ TFSMMLGDIN YRDAFLEPLF RNELAYPVLT FGQLIAFTMF VPIVLMNLLI 

       970        980        990       1000       1010       1020 
GLAVGDIAEV QKHASLKRIA MQVELHTNLE KKLPLWYLRK VDQRSTIVYP NRPRHGRMLR 

      1030       1040       1050       1060       1070       1080 
FFHYFLNMQE TRQEVPNIDT CLEMEILKQK YRLKDLTSLL EKQHELIKLI IQKMEIISET 

      1090       1100       1110       1120 
EDEDNHCSFQ DRFKKERLEQ MHSKWNFVLN AVKTKTHCSI SHPDF 

« Hide

References

« Hide 'large scale' references
[1]"ANKTM1, a TRP-like channel expressed in nociceptive neurons, is activated by cold temperatures."
Story G.M., Peier A.M., Reeve A.J., Eid S.R., Mosbacher J., Hricik T.R., Earley T.J., Hergarden A.C., Anderson D.A., Hwang S.W., McIntyre P., Jegla T., Bevan S., Patapoutian A.
Cell 112:819-829(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
Strain: C57BL/6.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Corpora quadrigemina.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[4]"TRPA1 is a candidate for the mechanosensitive transduction channel of vertebrate hair cells."
Corey D.P., Garcia-Anoveros J., Holt J.R., Kwan K.Y., Lin S.-Y., Vollrath M.A., Amalfitano A., Cheung E.-L.M., Derfler B.H., Duggan A., Geleoc G.S.G., Gray P.A., Hoffman M.P., Rehm H.L., Tamasauskas D., Zhang D.-S.
Nature 432:723-730(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[5]"Cold-sensitive, menthol-insensitive neurons in the murine sympathetic nervous system."
Smith M.P., Beacham D., Ensor E., Koltzenburg M.
NeuroReport 15:1399-1403(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[6]"Noxious cold ion channel TRPA1 is activated by pungent compounds and bradykinin."
Bandell M., Story G.M., Hwang S.W., Viswanath V., Eid S.R., Petrus M.J., Earley T.J., Patapoutian A.
Neuron 41:849-857(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Nociceptor and hair cell transducer properties of TRPA1, a channel for pain and hearing."
Nagata K., Duggan A., Kumar G., Garcia-Anoveros J.
J. Neurosci. 25:4052-4061(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
[8]"TRPA1 mediates the inflammatory actions of environmental irritants and proalgesic agents."
Bautista D.M., Jordt S.E., Nikai T., Tsuruda P.R., Read A.J., Poblete J., Yamoah E.N., Basbaum A.I., Julius D.
Cell 124:1269-1282(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
[9]"Identification of in vivo disulfide conformation of TRPA1 ion channel."
Wang L., Cvetkov T.L., Chance M.R., Moiseenkova-Bell V.Y.
J. Biol. Chem. 287:6169-6176(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: DISULFIDE BONDS.
[10]"Molecular architecture and subunit organization of TRPA1 ion channel revealed by electron microscopy."
Cvetkov T.L., Huynh K.W., Cohen M.R., Moiseenkova-Bell V.Y.
J. Biol. Chem. 286:38168-38176(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: ELECTRON MICROSCOPY (16 ANGSTROMS), SUBUNIT.
+Additional computationally mapped references.

Web resources

Protein Spotlight

The power behind pain - Issue 82 of May 2007

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY231177 mRNA. Translation: AAO43183.1.
AK045771 mRNA. Translation: BAC32487.1.
BC120563 mRNA. Translation: AAI20564.1.
BC131963 mRNA. Translation: AAI31964.1.
CCDSCCDS35516.1.
RefSeqNP_808449.1. NM_177781.4.
XP_006495588.1. XM_006495525.1.
UniGeneMm.186329.

3D structure databases

ProteinModelPortalQ8BLA8.
SMRQ8BLA8. Positions 30-640, 793-973.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBQ8BLA8.
ChEMBLCHEMBL1075310.
GuidetoPHARMACOLOGY485.

Protein family/group databases

TCDB1.A.4.6.1. the transient receptor potential ca(2+) channel (trp-cc) family.

PTM databases

PhosphoSiteQ8BLA8.

Proteomic databases

PRIDEQ8BLA8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000041447; ENSMUSP00000043594; ENSMUSG00000032769.
GeneID277328.
KEGGmmu:277328.
UCSCuc007ajc.1. mouse.

Organism-specific databases

CTD8989.
MGIMGI:3522699. Trpa1.

Phylogenomic databases

eggNOGCOG0666.
GeneTreeENSGT00740000115514.
HOGENOMHOG000044486.
HOVERGENHBG059027.
InParanoidQ8BLA8.
KOK04984.
OMAWLAYGFR.
OrthoDBEOG7P02H2.
PhylomeDBQ8BLA8.
TreeFamTF317264.

Gene expression databases

BgeeQ8BLA8.
GenevestigatorQ8BLA8.

Family and domain databases

Gene3D1.25.40.20. 4 hits.
InterProIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
PfamPF12796. Ank_2. 6 hits.
[Graphical view]
PRINTSPR01415. ANKYRIN.
SMARTSM00248. ANK. 14 hits.
[Graphical view]
SUPFAMSSF48403. SSF48403. 2 hits.
PROSITEPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 9 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio393737.
PROQ8BLA8.
SOURCESearch...

Entry information

Entry nameTRPA1_MOUSE
AccessionPrimary (citable) accession number: Q8BLA8
Secondary accession number(s): Q0VBN5
Entry history
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot