Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8BL66 (EEA1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Early endosome antigen 1
Gene names
Name:Eea1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1411 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking By similarity.

Subunit structure

Homodimer. Binds STX6. Binds RAB5A, RAB5B, RAB5C and RAB22A that have been activated by GTP-binding. Interacts with ERBB2 By similarity. Interacts with RAB31. May interact with PLEKHF2 By similarity. Ref.3

Subcellular location

Cytoplasm By similarity. Early endosome membrane; Peripheral membrane protein By similarity Ref.3.

Domain

The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns3P-enriched membranes is substantially increased in acidic conditions By similarity.

Sequence similarities

Contains 1 C2H2-type zinc finger.

Contains 1 FYVE-type zinc finger.

Sequence caution

The sequence BAC32647.1 differs from that shown. Reason: Erroneous termination at position 105. Translated as Tyr.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 14111411Early endosome antigen 1
PRO_0000098707

Regions

Zinc finger41 – 6424C2H2-type
Zinc finger1352 – 141059FYVE-type
Coiled coil78 – 13481271 Potential
Compositional bias396 – 706311Gln/Glu/Lys-rich

Amino acid modifications

Modified residue701Phosphoserine By similarity

Experimental info

Sequence conflict2251V → I in BAC32647. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q8BL66 [UniParc].

Last modified September 27, 2005. Version 2.
Checksum: 2365A51EF92019FD

FASTA1,411160,915
        10         20         30         40         50         60 
MFRRILQRTP GRVGSQGSDL DSSATPINTV DVNNESSSEG FICPQCMKSL GSADELFKHY 

        70         80         90        100        110        120 
QAVHDAGNDS GHGGEAGLAL TRDDITLLRQ EVQDLQASLK EEKWYSEELK KELEKYQGLQ 

       130        140        150        160        170        180 
QQEAKSDGLV TDSSAELQAL EQQLEEAQTE NFNIKQMKDL FEQKAAQLAT EIADIKSKYD 

       190        200        210        220        230        240 
EEKSLRAAAE QKVTHLTEDL NKQTTVIQDL KTELLQRPGI EDVAVLKKEL VQVQTLMDNM 

       250        260        270        280        290        300 
TLERERESEK LKDECKKLQS EHAHLEATIN QLRSELAKGP QEVAVYVQEI QKLKGSINEL 

       310        320        330        340        350        360 
TQKNQNLTEK LQKKDLDYTH LEEKHNEESA SRKTLQASLH QRDLDCQQLQ ARLTASESSL 

       370        380        390        400        410        420 
QRAQGELSEK AEAAQKLREE LREVESTRQH LKVEVKQLQQ QREEKEQHGL QLQGEVSQLH 

       430        440        450        460        470        480 
CKLLETERQL GEAHGRLKEQ RQLSSEKLME KEQQVADLQL KLSRLEEQLK EKVTNSTELQ 

       490        500        510        520        530        540 
HQLEKSKQQH QEQQALQQSA TAKLREAQND LEQVLRQIGD KDQKIQNLEA LLQKGKESVS 

       550        560        570        580        590        600 
LLEKEREDLY AKIQAGEGET AVLNQLQEKN HALQQQLTQL TEKLKNQSES HKQAEENLHD 

       610        620        630        640        650        660 
QVQEQKAHLR AAQDRVLSLE TSVSELSSQL NESKEKVSQL DIQIKAKTEL LLSAEAAKAA 

       670        680        690        700        710        720 
QRADLQNHLD TAQHALQDKQ QELNKVSVQL DQLTAKFQEK QEHCIQLESH LKDHKEKHLS 

       730        740        750        760        770        780 
LEQKVEDLEG HIKKLEADAL EVKASKEQAL QSLQQQRQLS TDLELRNAEL SRELQEQEEV 

       790        800        810        820        830        840 
VSCTKLDLQN KSEILENIKQ TLTKKEEENV VLKQEFEKLS QDSKTQHKEL GDRMQAAVTE 

       850        860        870        880        890        900 
LTAVKAQKDA LLAELSTTKE KLSKVSDSLK NSKSEFEKEN QKGKAAVLDL EKACKELKHQ 

       910        920        930        940        950        960 
LQVQAESALK EQEDLKKSLE KEKETSQQLK IELNSVKGEV SQAQNTLKQK EKDEQQLQGT 

       970        980        990       1000       1010       1020 
INQLKQSAEQ KKKQIEALQG EVKNAVSQKT VLENKLQQQS SQAAQELAAE KGKLSALQSN 

      1030       1040       1050       1060       1070       1080 
YEKCQADLKQ LQSDLYGKES ELLATRQDLK SVEEKLTLAQ EDLISNRNQI GNQNKSIQEL 

      1090       1100       1110       1120       1130       1140 
QAAKASLEQD SAKKEALLKE QSKALEDAQR EKSVKEKELV AEKSKLAEME EIKCRQEKEI 

      1150       1160       1170       1180       1190       1200 
TKLNEELKSH KQESIKEITN LKDAKQLLIQ QKLELQGRVD SLKAALEQEK ESQQLMREQV 

      1210       1220       1230       1240       1250       1260 
KKEEEKRKEE FSEKEAKLHS EIKEKEAGMK KHEENEAKLT MQVTTLNENL GTVKKEWQSS 

      1270       1280       1290       1300       1310       1320 
QRRVSELEKQ TDDLRGEIAV LEATVQNNQD ERRALLERCL KGEGEIEKLQ TKALELQRKL 

      1330       1340       1350       1360       1370       1380 
DNTTAAVQEL GRENQSLQIK HTQALNRKWA EDNEVQNCMS CGKCFSVTVR RHHCRQCGNI 

      1390       1400       1410 
FCAECSTKNA LTPSSKKPVR VCDACFNDLQ G 

« Hide

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-946.
Strain: C57BL/6J.
Tissue: Brain.
[3]"p75 neurotrophin receptor regulates glucose homeostasis and insulin sensitivity."
Baeza-Raja B., Li P., Le Moan N., Sachs B.D., Schachtrup C., Davalos D., Vagena E., Bridges D., Kim C., Saltiel A.R., Olefsky J.M., Akassoglou K.
Proc. Natl. Acad. Sci. U.S.A. 109:5838-5843(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH RAB31.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC075637 mRNA. Translation: AAH75637.1.
AK046231 mRNA. Translation: BAC32647.1. Sequence problems.
CCDSCCDS36042.1.
RefSeqNP_001001932.1. NM_001001932.3.
UniGeneMm.210035.
Mm.490373.

3D structure databases

ProteinModelPortalQ8BL66.
SMRQ8BL66. Positions 36-69, 1289-1411.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid229728. 3 interactions.
IntActQ8BL66. 11 interactions.
MINTMINT-1865447.

PTM databases

PhosphoSiteQ8BL66.

2D gel databases

REPRODUCTION-2DPAGEIPI00453776.

Proteomic databases

MaxQBQ8BL66.
PaxDbQ8BL66.
PRIDEQ8BL66.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000053484; ENSMUSP00000061493; ENSMUSG00000036499.
GeneID216238.
KEGGmmu:216238.
UCSCuc007gwu.1. mouse.

Organism-specific databases

CTD8411.
MGIMGI:2442192. Eea1.

Phylogenomic databases

eggNOGNOG12793.
GeneTreeENSGT00730000110715.
HOGENOMHOG000112329.
HOVERGENHBG039440.
InParanoidQ8BL66.
KOK12478.
OMAKHYEVVH.
OrthoDBEOG754HNM.
PhylomeDBQ8BL66.
TreeFamTF329698.

Gene expression databases

BgeeQ8BL66.
CleanExMM_EEA1.
GenevestigatorQ8BL66.

Family and domain databases

Gene3D3.30.40.10. 1 hit.
InterProIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamPF01363. FYVE. 1 hit.
[Graphical view]
SMARTSM00064. FYVE. 1 hit.
SM00355. ZnF_C2H2. 1 hit.
[Graphical view]
SUPFAMSSF57903. SSF57903. 1 hit.
PROSITEPS50178. ZF_FYVE. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio375093.
PROQ8BL66.
SOURCESearch...

Entry information

Entry nameEEA1_MOUSE
AccessionPrimary (citable) accession number: Q8BL66
Secondary accession number(s): Q6DIC2
Entry history
Integrated into UniProtKB/Swiss-Prot: August 22, 2003
Last sequence update: September 27, 2005
Last modified: July 9, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot