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Q8BL66

- EEA1_MOUSE

UniProt

Q8BL66 - EEA1_MOUSE

Protein

Early endosome antigen 1

Gene

Eea1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 2 (27 Sep 2005)
      Previous versions | rss
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    Functioni

    Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri41 – 6424C2H2-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri1352 – 141059FYVE-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. 1-phosphatidylinositol binding Source: UniProtKB
    2. metal ion binding Source: UniProtKB-KW
    3. protein binding Source: MGI

    GO - Biological processi

    1. endocytosis Source: UniProtKB
    2. vesicle fusion Source: Ensembl

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Early endosome antigen 1
    Gene namesi
    Name:Eea1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:2442192. Eea1.

    Subcellular locationi

    Cytoplasm By similarity. Early endosome membrane By similarity; Peripheral membrane protein By similarity

    GO - Cellular componenti

    1. axonal spine Source: MGI
    2. cytoplasmic vesicle Source: MGI
    3. cytosol Source: UniProtKB
    4. early endosome Source: UniProtKB
    5. early endosome membrane Source: UniProtKB-SubCell
    6. endosome Source: MGI
    7. extrinsic component of plasma membrane Source: UniProtKB
    8. recycling endosome Source: Ensembl
    9. serine-pyruvate aminotransferase complex Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Endosome, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 14111411Early endosome antigen 1PRO_0000098707Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei70 – 701PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ8BL66.
    PaxDbiQ8BL66.
    PRIDEiQ8BL66.

    2D gel databases

    REPRODUCTION-2DPAGEIPI00453776.

    PTM databases

    PhosphoSiteiQ8BL66.

    Expressioni

    Gene expression databases

    BgeeiQ8BL66.
    CleanExiMM_EEA1.
    GenevestigatoriQ8BL66.

    Interactioni

    Subunit structurei

    Homodimer. Binds STX6. Binds RAB5A, RAB5B, RAB5C and RAB22A that have been activated by GTP-binding. Interacts with ERBB2 By similarity. Interacts with RAB31. May interact with PLEKHF2 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi229728. 3 interactions.
    IntActiQ8BL66. 11 interactions.
    MINTiMINT-1865447.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BL66.
    SMRiQ8BL66. Positions 36-69, 1289-1411.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili78 – 13481271Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi396 – 706311Gln/Glu/Lys-richAdd
    BLAST

    Domaini

    The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns3P-enriched membranes is substantially increased in acidic conditions By similarity.By similarity

    Sequence similaritiesi

    Contains 1 C2H2-type zinc finger.PROSITE-ProRule annotation
    Contains 1 FYVE-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri41 – 6424C2H2-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri1352 – 141059FYVE-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Coiled coil, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG12793.
    GeneTreeiENSGT00730000110715.
    HOGENOMiHOG000112329.
    HOVERGENiHBG039440.
    InParanoidiQ8BL66.
    KOiK12478.
    OMAiKHYEVVH.
    OrthoDBiEOG754HNM.
    PhylomeDBiQ8BL66.
    TreeFamiTF329698.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    InterProiIPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR000306. Znf_FYVE.
    IPR017455. Znf_FYVE-rel.
    IPR011011. Znf_FYVE_PHD.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view]
    PfamiPF01363. FYVE. 1 hit.
    [Graphical view]
    SMARTiSM00064. FYVE. 1 hit.
    SM00355. ZnF_C2H2. 1 hit.
    [Graphical view]
    SUPFAMiSSF57903. SSF57903. 1 hit.
    PROSITEiPS50178. ZF_FYVE. 1 hit.
    PS00028. ZINC_FINGER_C2H2_1. 1 hit.
    PS50157. ZINC_FINGER_C2H2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8BL66-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFRRILQRTP GRVGSQGSDL DSSATPINTV DVNNESSSEG FICPQCMKSL     50
    GSADELFKHY QAVHDAGNDS GHGGEAGLAL TRDDITLLRQ EVQDLQASLK 100
    EEKWYSEELK KELEKYQGLQ QQEAKSDGLV TDSSAELQAL EQQLEEAQTE 150
    NFNIKQMKDL FEQKAAQLAT EIADIKSKYD EEKSLRAAAE QKVTHLTEDL 200
    NKQTTVIQDL KTELLQRPGI EDVAVLKKEL VQVQTLMDNM TLERERESEK 250
    LKDECKKLQS EHAHLEATIN QLRSELAKGP QEVAVYVQEI QKLKGSINEL 300
    TQKNQNLTEK LQKKDLDYTH LEEKHNEESA SRKTLQASLH QRDLDCQQLQ 350
    ARLTASESSL QRAQGELSEK AEAAQKLREE LREVESTRQH LKVEVKQLQQ 400
    QREEKEQHGL QLQGEVSQLH CKLLETERQL GEAHGRLKEQ RQLSSEKLME 450
    KEQQVADLQL KLSRLEEQLK EKVTNSTELQ HQLEKSKQQH QEQQALQQSA 500
    TAKLREAQND LEQVLRQIGD KDQKIQNLEA LLQKGKESVS LLEKEREDLY 550
    AKIQAGEGET AVLNQLQEKN HALQQQLTQL TEKLKNQSES HKQAEENLHD 600
    QVQEQKAHLR AAQDRVLSLE TSVSELSSQL NESKEKVSQL DIQIKAKTEL 650
    LLSAEAAKAA QRADLQNHLD TAQHALQDKQ QELNKVSVQL DQLTAKFQEK 700
    QEHCIQLESH LKDHKEKHLS LEQKVEDLEG HIKKLEADAL EVKASKEQAL 750
    QSLQQQRQLS TDLELRNAEL SRELQEQEEV VSCTKLDLQN KSEILENIKQ 800
    TLTKKEEENV VLKQEFEKLS QDSKTQHKEL GDRMQAAVTE LTAVKAQKDA 850
    LLAELSTTKE KLSKVSDSLK NSKSEFEKEN QKGKAAVLDL EKACKELKHQ 900
    LQVQAESALK EQEDLKKSLE KEKETSQQLK IELNSVKGEV SQAQNTLKQK 950
    EKDEQQLQGT INQLKQSAEQ KKKQIEALQG EVKNAVSQKT VLENKLQQQS 1000
    SQAAQELAAE KGKLSALQSN YEKCQADLKQ LQSDLYGKES ELLATRQDLK 1050
    SVEEKLTLAQ EDLISNRNQI GNQNKSIQEL QAAKASLEQD SAKKEALLKE 1100
    QSKALEDAQR EKSVKEKELV AEKSKLAEME EIKCRQEKEI TKLNEELKSH 1150
    KQESIKEITN LKDAKQLLIQ QKLELQGRVD SLKAALEQEK ESQQLMREQV 1200
    KKEEEKRKEE FSEKEAKLHS EIKEKEAGMK KHEENEAKLT MQVTTLNENL 1250
    GTVKKEWQSS QRRVSELEKQ TDDLRGEIAV LEATVQNNQD ERRALLERCL 1300
    KGEGEIEKLQ TKALELQRKL DNTTAAVQEL GRENQSLQIK HTQALNRKWA 1350
    EDNEVQNCMS CGKCFSVTVR RHHCRQCGNI FCAECSTKNA LTPSSKKPVR 1400
    VCDACFNDLQ G 1411
    Length:1,411
    Mass (Da):160,915
    Last modified:September 27, 2005 - v2
    Checksum:i2365A51EF92019FD
    GO

    Sequence cautioni

    The sequence BAC32647.1 differs from that shown. Reason: Erroneous termination at position 105. Translated as Tyr.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti225 – 2251V → I in BAC32647. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC075637 mRNA. Translation: AAH75637.1.
    AK046231 mRNA. Translation: BAC32647.1. Sequence problems.
    CCDSiCCDS36042.1.
    RefSeqiNP_001001932.1. NM_001001932.3.
    UniGeneiMm.210035.
    Mm.490373.

    Genome annotation databases

    EnsembliENSMUST00000053484; ENSMUSP00000061493; ENSMUSG00000036499.
    GeneIDi216238.
    KEGGimmu:216238.
    UCSCiuc007gwu.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC075637 mRNA. Translation: AAH75637.1 .
    AK046231 mRNA. Translation: BAC32647.1 . Sequence problems.
    CCDSi CCDS36042.1.
    RefSeqi NP_001001932.1. NM_001001932.3.
    UniGenei Mm.210035.
    Mm.490373.

    3D structure databases

    ProteinModelPortali Q8BL66.
    SMRi Q8BL66. Positions 36-69, 1289-1411.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 229728. 3 interactions.
    IntActi Q8BL66. 11 interactions.
    MINTi MINT-1865447.

    PTM databases

    PhosphoSitei Q8BL66.

    2D gel databases

    REPRODUCTION-2DPAGE IPI00453776.

    Proteomic databases

    MaxQBi Q8BL66.
    PaxDbi Q8BL66.
    PRIDEi Q8BL66.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000053484 ; ENSMUSP00000061493 ; ENSMUSG00000036499 .
    GeneIDi 216238.
    KEGGi mmu:216238.
    UCSCi uc007gwu.1. mouse.

    Organism-specific databases

    CTDi 8411.
    MGIi MGI:2442192. Eea1.

    Phylogenomic databases

    eggNOGi NOG12793.
    GeneTreei ENSGT00730000110715.
    HOGENOMi HOG000112329.
    HOVERGENi HBG039440.
    InParanoidi Q8BL66.
    KOi K12478.
    OMAi KHYEVVH.
    OrthoDBi EOG754HNM.
    PhylomeDBi Q8BL66.
    TreeFami TF329698.

    Miscellaneous databases

    NextBioi 375093.
    PROi Q8BL66.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8BL66.
    CleanExi MM_EEA1.
    Genevestigatori Q8BL66.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    InterProi IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR000306. Znf_FYVE.
    IPR017455. Znf_FYVE-rel.
    IPR011011. Znf_FYVE_PHD.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view ]
    Pfami PF01363. FYVE. 1 hit.
    [Graphical view ]
    SMARTi SM00064. FYVE. 1 hit.
    SM00355. ZnF_C2H2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57903. SSF57903. 1 hit.
    PROSITEi PS50178. ZF_FYVE. 1 hit.
    PS00028. ZINC_FINGER_C2H2_1. 1 hit.
    PS50157. ZINC_FINGER_C2H2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-946.
      Strain: C57BL/6J.
      Tissue: Brain.
    3. Cited for: SUBCELLULAR LOCATION, INTERACTION WITH RAB31.

    Entry informationi

    Entry nameiEEA1_MOUSE
    AccessioniPrimary (citable) accession number: Q8BL66
    Secondary accession number(s): Q6DIC2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 22, 2003
    Last sequence update: September 27, 2005
    Last modified: October 1, 2014
    This is version 102 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3