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Protein

Testican-3

Gene

Spock3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

May participate in diverse steps of neurogenesis. Inhibits the processing of pro-matrix metalloproteinase 2 (MMP-2) by MT1-MMP and MT3-MMP. May interfere with tumor invasion (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Metalloenzyme inhibitor, Metalloprotease inhibitor, Protease inhibitor

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiR-MMU-1592389. Activation of Matrix Metalloproteinases.

Protein family/group databases

MEROPSiI31.007.

Names & Taxonomyi

Protein namesi
Recommended name:
Testican-3
Alternative name(s):
SPARC/osteonectin, CWCV, and Kazal-like domains proteoglycan 3
Gene namesi
Name:Spock3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1920152. Spock3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000002670423 – 436Testican-3Add BLAST414

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi90 ↔ 101By similarity
Disulfide bondi95 ↔ 111By similarity
Disulfide bondi139 ↔ 169By similarity
Disulfide bondi142 ↔ 162By similarity
Disulfide bondi151 ↔ 183By similarity
Disulfide bondi317 ↔ 341By similarity
Disulfide bondi352 ↔ 359By similarity
Disulfide bondi361 ↔ 380By similarity
Glycosylationi387O-linked (Xyl...) (glycosaminoglycan)Sequence analysis1
Glycosylationi392O-linked (Xyl...) (glycosaminoglycan)Sequence analysis1

Post-translational modificationi

Contains chondroitin sulfate and heparan sulfate O-linked oligosaccharides.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Heparan sulfate, Proteoglycan

Proteomic databases

PaxDbiQ8BKV0.
PRIDEiQ8BKV0.

PTM databases

iPTMnetiQ8BKV0.
PhosphoSitePlusiQ8BKV0.

Expressioni

Tissue specificityi

Expressed in brain.

Gene expression databases

BgeeiENSMUSG00000054162.
GenevisibleiQ8BKV0. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000091192.

Structurei

3D structure databases

ProteinModelPortaliQ8BKV0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini133 – 185Kazal-likePROSITE-ProRule annotationAdd BLAST53
Domaini314 – 380Thyroglobulin type-1PROSITE-ProRule annotationAdd BLAST67

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi394 – 436Asp-richAdd BLAST43

Sequence similaritiesi

Contains 1 Kazal-like domain.PROSITE-ProRule annotation
Contains 1 thyroglobulin type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3555. Eukaryota.
ENOG410Y6A8. LUCA.
GeneTreeiENSGT00510000046429.
HOGENOMiHOG000293295.
HOVERGENiHBG026595.
InParanoidiQ8BKV0.
KOiK08136.
OMAiRLTHSMK.
OrthoDBiEOG091G086I.
TreeFamiTF317779.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
4.10.800.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR002350. Kazal_dom.
IPR019577. SPARC/Testican_Ca-bd-dom.
IPR000716. Thyroglobulin_1.
[Graphical view]
PfamiPF07648. Kazal_2. 1 hit.
PF10591. SPARC_Ca_bdg. 1 hit.
PF00086. Thyroglobulin_1. 1 hit.
[Graphical view]
SMARTiSM00280. KAZAL. 1 hit.
SM00211. TY. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF57610. SSF57610. 1 hit.
PROSITEiPS51465. KAZAL_2. 1 hit.
PS00484. THYROGLOBULIN_1_1. 1 hit.
PS51162. THYROGLOBULIN_1_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BKV0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLKVSALLCV CAAAWCSQTL AAAAAVAVAG GRSDGGNFLD EKQWLTTISQ
60 70 80 90 100
YDKEVGQWNK FRDEVEDDYF RTWNPGKPFD QALDPAKDPC LKTKCSRHKV
110 120 130 140 150
CITQDAQTAL CISHRRLTHS MKEVGGSHKQ WRGLPSSTCK PCPIAYASPV
160 170 180 190 200
CGSDGHSYSS QCKLEYQACV LGKQISIKCE GRCPCPSDKS MNIGRNVKRA
210 220 230 240 250
CSDLEFREVA NRLRDWFKAL HESGSQNKKT KALLRPERSR FDTSILPICK
260 270 280 290 300
DSLGWMFNRL DTNYDLLLDQ SELGSIYLDK NEQCTKAFFN SCDTYKDSLI
310 320 330 340 350
SNNEWCYCFQ RQQDPPCHTE LSNIQKRQGI KKLLGQYIPL CDEDGYYKPT
360 370 380 390 400
QCHGSVGQCW CVDRYGNEVV GSRINGVADC AIDFEISGDF ASGDFREWTD
410 420 430
DEGEEDDIMN DKDDIEDDDE DEGDDDDDGD VHDGYI
Length:436
Mass (Da):49,098
Last modified:May 10, 2005 - v2
Checksum:i6FFD8A13815E9BC5
GO
Isoform 2 (identifier: Q8BKV0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     64-66: Missing.

Show »
Length:433
Mass (Da):48,740
Checksum:iC1F293C7B7271799
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01363364 – 66Missing in isoform 2. 1 Publication3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ278998 mRNA. Translation: CAC08506.1.
AK013644 mRNA. Translation: BAB28935.1.
AK049843 mRNA. Translation: BAC33951.1.
BC017601 mRNA. Translation: AAH17601.1.
BC053334 mRNA. Translation: AAH53334.1.
CCDSiCCDS22326.1. [Q8BKV0-1]
CCDS57622.1. [Q8BKV0-2]
RefSeqiNP_001239549.1. NM_001252620.1. [Q8BKV0-1]
NP_001239550.1. NM_001252621.1. [Q8BKV0-2]
NP_076178.1. NM_023689.3. [Q8BKV0-1]
XP_006509581.1. XM_006509518.1. [Q8BKV0-2]
UniGeneiMm.334552.

Genome annotation databases

EnsembliENSMUST00000093480; ENSMUSP00000091192; ENSMUSG00000054162. [Q8BKV0-1]
ENSMUST00000117377; ENSMUSP00000113797; ENSMUSG00000054162. [Q8BKV0-2]
ENSMUST00000118003; ENSMUSP00000113683; ENSMUSG00000054162. [Q8BKV0-1]
ENSMUST00000119068; ENSMUSP00000112930; ENSMUSG00000054162. [Q8BKV0-1]
GeneIDi72902.
KEGGimmu:72902.
UCSCiuc009lur.1. mouse. [Q8BKV0-2]
uc009lus.2. mouse. [Q8BKV0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ278998 mRNA. Translation: CAC08506.1.
AK013644 mRNA. Translation: BAB28935.1.
AK049843 mRNA. Translation: BAC33951.1.
BC017601 mRNA. Translation: AAH17601.1.
BC053334 mRNA. Translation: AAH53334.1.
CCDSiCCDS22326.1. [Q8BKV0-1]
CCDS57622.1. [Q8BKV0-2]
RefSeqiNP_001239549.1. NM_001252620.1. [Q8BKV0-1]
NP_001239550.1. NM_001252621.1. [Q8BKV0-2]
NP_076178.1. NM_023689.3. [Q8BKV0-1]
XP_006509581.1. XM_006509518.1. [Q8BKV0-2]
UniGeneiMm.334552.

3D structure databases

ProteinModelPortaliQ8BKV0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000091192.

Protein family/group databases

MEROPSiI31.007.

PTM databases

iPTMnetiQ8BKV0.
PhosphoSitePlusiQ8BKV0.

Proteomic databases

PaxDbiQ8BKV0.
PRIDEiQ8BKV0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000093480; ENSMUSP00000091192; ENSMUSG00000054162. [Q8BKV0-1]
ENSMUST00000117377; ENSMUSP00000113797; ENSMUSG00000054162. [Q8BKV0-2]
ENSMUST00000118003; ENSMUSP00000113683; ENSMUSG00000054162. [Q8BKV0-1]
ENSMUST00000119068; ENSMUSP00000112930; ENSMUSG00000054162. [Q8BKV0-1]
GeneIDi72902.
KEGGimmu:72902.
UCSCiuc009lur.1. mouse. [Q8BKV0-2]
uc009lus.2. mouse. [Q8BKV0-1]

Organism-specific databases

CTDi50859.
MGIiMGI:1920152. Spock3.

Phylogenomic databases

eggNOGiKOG3555. Eukaryota.
ENOG410Y6A8. LUCA.
GeneTreeiENSGT00510000046429.
HOGENOMiHOG000293295.
HOVERGENiHBG026595.
InParanoidiQ8BKV0.
KOiK08136.
OMAiRLTHSMK.
OrthoDBiEOG091G086I.
TreeFamiTF317779.

Enzyme and pathway databases

ReactomeiR-MMU-1592389. Activation of Matrix Metalloproteinases.

Miscellaneous databases

PROiQ8BKV0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000054162.
GenevisibleiQ8BKV0. MM.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
4.10.800.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR002350. Kazal_dom.
IPR019577. SPARC/Testican_Ca-bd-dom.
IPR000716. Thyroglobulin_1.
[Graphical view]
PfamiPF07648. Kazal_2. 1 hit.
PF10591. SPARC_Ca_bdg. 1 hit.
PF00086. Thyroglobulin_1. 1 hit.
[Graphical view]
SMARTiSM00280. KAZAL. 1 hit.
SM00211. TY. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF57610. SSF57610. 1 hit.
PROSITEiPS51465. KAZAL_2. 1 hit.
PS00484. THYROGLOBULIN_1_1. 1 hit.
PS51162. THYROGLOBULIN_1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTICN3_MOUSE
AccessioniPrimary (citable) accession number: Q8BKV0
Secondary accession number(s): Q9ER59
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: November 2, 2016
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.