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Q8BKT6

- TSN12_MOUSE

UniProt

Q8BKT6 - TSN12_MOUSE

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Protein

Tetraspanin-12

Gene

Tspan12

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Regulator of cell surface receptor signal transduction. Acts as a regulator of membrane proteinases such as ADAM10 and MMP14/MT1-MMP. Activates ADAM10-dependent cleavage activity of amyloid precursor protein (APP). Activates MMP14/MT1-MMP-dependent cleavage activity (By similarity). Plays a central role in retinal vascularization by regulating norrin (NDP) signal transduction. Acts in concert with norrin (NDP) to promote FZD4 multimerization and subsequent activation of FZD4, leading to promote accumulation of beta-catenin (CTNNB1) and stimulate LEF/TCF-mediated transcriptional programs. Suprisingly, it only activate the norrin (NDP)-dependent activation of FZD4, while it does not activate the Wnt-dependent activation of FZD4, suggesting the existence of a Wnt-independent signaling that also promote accumulation the beta-catenin (CTNNB1).By similarity1 Publication

GO - Biological processi

  1. angiogenesis Source: UniProtKB-KW
  2. cell surface receptor signaling pathway Source: UniProtKB
  3. regulation of angiogenesis Source: UniProtKB
  4. retina layer formation Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Angiogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Tetraspanin-12
Short name:
Tspan-12
Alternative name(s):
Transmembrane 4 superfamily member 12
Gene namesi
Name:Tspan12
Synonyms:Tm4sf12
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:1889818. Tspan12.

Subcellular locationi

Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

GO - Cellular componenti

  1. integral component of plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are viable and fertile but display defects in retinal vascularization. In retinas between P5 and P12, the centrifugal outgrowth of the nerve fiber layer (NFL) vasculature is moderately delayed in retinas. At P11, vertical sprouts and plexiform layer (OPL) capillaries appear in wild-type mice, whereas both are completely absent in mutant mice. In adult mutant mice, the plexiform layer (OPL) remains avascular, confirming that the defect is not transient. The thickness of the outer nuclear layer in retinas is consistently reduced in adult mutant but not neonatal mice, indicating that neural cells are secondarily affected by the vascular defects.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 305305Tetraspanin-12PRO_0000290009Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi9 – 91S-palmitoyl cysteineBy similarity
Lipidationi12 – 121S-palmitoyl cysteineBy similarity
Lipidationi83 – 831S-palmitoyl cysteineBy similarity

Post-translational modificationi

Palmitoylated; required for interaction with ADAM10.By similarity

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PRIDEiQ8BKT6.

PTM databases

PhosphoSiteiQ8BKT6.

Expressioni

Tissue specificityi

Expressed in the neonatal retinal vasculature but not other retinal tissues. Also detected in the neonatal meningeal vasculature and in nonvascular cell types, such as the smooth muscle cells in the neonatal intestine.1 Publication

Gene expression databases

BgeeiQ8BKT6.
CleanExiMM_TSPAN12.
ExpressionAtlasiQ8BKT6. baseline and differential.
GenevestigatoriQ8BKT6.

Interactioni

Subunit structurei

Interacts (when palmitoylated) with ADAM10. Interacts with MMP14/MT1-MMP (By similarity). Component of a complex, at least composed of TSPAN12, FZD4 and norrin (NDP).By similarity1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000031678.

Structurei

3D structure databases

ProteinModelPortaliQ8BKT6.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1212CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini34 – 5926ExtracellularSequence AnalysisAdd
BLAST
Topological domaini81 – 899CytoplasmicSequence Analysis
Topological domaini111 – 224114ExtracellularSequence AnalysisAdd
BLAST
Topological domaini246 – 30560CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei13 – 3321HelicalSequence AnalysisAdd
BLAST
Transmembranei60 – 8021HelicalSequence AnalysisAdd
BLAST
Transmembranei90 – 11021HelicalSequence AnalysisAdd
BLAST
Transmembranei225 – 24521HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the tetraspanin (TM4SF) family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG257008.
GeneTreeiENSGT00510000047764.
HOGENOMiHOG000252919.
HOVERGENiHBG054253.
InParanoidiQ8BKT6.
KOiK17355.
OMAiTYEQEIM.
OrthoDBiEOG7SFHX2.
PhylomeDBiQ8BKT6.
TreeFamiTF316345.

Family and domain databases

InterProiIPR000301. Tetraspanin.
IPR018499. Tetraspanin/Peripherin.
IPR018503. Tetraspanin_CS.
IPR008952. Tetraspanin_EC2.
[Graphical view]
PfamiPF00335. Tetraspannin. 1 hit.
[Graphical view]
PIRSFiPIRSF002419. Tetraspanin. 1 hit.
PRINTSiPR00259. TMFOUR.
SUPFAMiSSF48652. SSF48652. 1 hit.
PROSITEiPS00421. TM4_1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8BKT6-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAREDSVKCL RCLLYALNLL FWLMSISVLA VSAWMRDYLN NVLTLTAETR
60 70 80 90 100
VEEAVILTYF PVVHPVMIAV CCFLIIVGML GYCGTVKRNL LLLAWYFGTL
110 120 130 140 150
LVIFCVELAC GVWTYEQEVM VPVQWSDMVT LKARMTNYGL PRYRWLTHAW
160 170 180 190 200
NYFQREFKCC GVVYFTDWLE MTEMDWPPDS CCVREFPGCS KQAHQEDLSD
210 220 230 240 250
LYQEGCGKKM YSFLRGTKQL QVLRFLGISI GVTQILAMIL TITLLWALYY
260 270 280 290 300
DRREPGTDQM LSLKNDTSQH LSCHSVELLK PSLSRIFEHT SMANSFNTHF

EMEEL
Length:305
Mass (Da):35,408
Last modified:March 1, 2003 - v1
Checksum:i1C5C36D37F002F06
GO
Isoform 2 (identifier: Q8BKT6-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     156-203: Missing.

Note: No experimental confirmation available.

Show »
Length:257
Mass (Da):29,757
Checksum:i63E3157D3EC429C2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti8 – 81K → E in BAC28354. (PubMed:16141072)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei156 – 20348Missing in isoform 2. 1 PublicationVSP_038526Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK033554 mRNA. Translation: BAC28354.1.
AK050737 mRNA. Translation: BAC34399.1.
CH466533 Genomic DNA. Translation: EDL13857.1.
BC065152 mRNA. Translation: AAH65152.1.
BC068240 mRNA. Translation: AAH68240.1.
CCDSiCCDS19935.1. [Q8BKT6-1]
RefSeqiNP_766595.1. NM_173007.3. [Q8BKT6-1]
XP_006505153.1. XM_006505090.1. [Q8BKT6-1]
XP_006505154.1. XM_006505091.1. [Q8BKT6-1]
UniGeneiMm.21950.

Genome annotation databases

EnsembliENSMUST00000031678; ENSMUSP00000031678; ENSMUSG00000029669. [Q8BKT6-1]
ENSMUST00000120965; ENSMUSP00000113384; ENSMUSG00000029669. [Q8BKT6-2]
GeneIDi269831.
KEGGimmu:269831.
UCSCiuc009bar.1. mouse. [Q8BKT6-1]
uc012eij.1. mouse. [Q8BKT6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK033554 mRNA. Translation: BAC28354.1 .
AK050737 mRNA. Translation: BAC34399.1 .
CH466533 Genomic DNA. Translation: EDL13857.1 .
BC065152 mRNA. Translation: AAH65152.1 .
BC068240 mRNA. Translation: AAH68240.1 .
CCDSi CCDS19935.1. [Q8BKT6-1 ]
RefSeqi NP_766595.1. NM_173007.3. [Q8BKT6-1 ]
XP_006505153.1. XM_006505090.1. [Q8BKT6-1 ]
XP_006505154.1. XM_006505091.1. [Q8BKT6-1 ]
UniGenei Mm.21950.

3D structure databases

ProteinModelPortali Q8BKT6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000031678.

PTM databases

PhosphoSitei Q8BKT6.

Proteomic databases

PRIDEi Q8BKT6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000031678 ; ENSMUSP00000031678 ; ENSMUSG00000029669 . [Q8BKT6-1 ]
ENSMUST00000120965 ; ENSMUSP00000113384 ; ENSMUSG00000029669 . [Q8BKT6-2 ]
GeneIDi 269831.
KEGGi mmu:269831.
UCSCi uc009bar.1. mouse. [Q8BKT6-1 ]
uc012eij.1. mouse. [Q8BKT6-2 ]

Organism-specific databases

CTDi 23554.
MGIi MGI:1889818. Tspan12.

Phylogenomic databases

eggNOGi NOG257008.
GeneTreei ENSGT00510000047764.
HOGENOMi HOG000252919.
HOVERGENi HBG054253.
InParanoidi Q8BKT6.
KOi K17355.
OMAi TYEQEIM.
OrthoDBi EOG7SFHX2.
PhylomeDBi Q8BKT6.
TreeFami TF316345.

Miscellaneous databases

NextBioi 393053.
PROi Q8BKT6.
SOURCEi Search...

Gene expression databases

Bgeei Q8BKT6.
CleanExi MM_TSPAN12.
ExpressionAtlasi Q8BKT6. baseline and differential.
Genevestigatori Q8BKT6.

Family and domain databases

InterProi IPR000301. Tetraspanin.
IPR018499. Tetraspanin/Peripherin.
IPR018503. Tetraspanin_CS.
IPR008952. Tetraspanin_EC2.
[Graphical view ]
Pfami PF00335. Tetraspannin. 1 hit.
[Graphical view ]
PIRSFi PIRSF002419. Tetraspanin. 1 hit.
PRINTSi PR00259. TMFOUR.
SUPFAMi SSF48652. SSF48652. 1 hit.
PROSITEi PS00421. TM4_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Colon.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6 and Czech II.
    Tissue: Brain and Mammary tumor.
  4. "TSPAN12 regulates retinal vascular development by promoting Norrin-but not Wnt-induced FZD4/beta-catenin signaling."
    Junge H.J., Yang S., Burton J.B., Paes K., Shu X., French D.M., Costa M., Rice D.S., Ye W.
    Cell 139:299-311(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, IDENTIFICATION IN A COMPLEX WITH FZD4 AND NDP, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiTSN12_MOUSE
AccessioniPrimary (citable) accession number: Q8BKT6
Secondary accession number(s): Q6P1C3, Q8BZU1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 12, 2007
Last sequence update: March 1, 2003
Last modified: October 29, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3