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Protein

GC-rich sequence DNA-binding factor 2

Gene

Gcfc2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Factor that represses transcription. It binds to the GC-rich sequences (5'-GCGGGGC-3') present in the epidermal growth factor receptor, beta-actin, and calcium-dependent protease promoters. Involved in pre-mRNA splicing through regulating spliceosome C complex formation. May play a role during late-stage splicing events and turnover of excised inrons (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

mRNA processing, mRNA splicing, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
GC-rich sequence DNA-binding factor 2
Alternative name(s):
GC-rich sequence DNA-binding factor
Transcription factor 9
Short name:
TCF-9
Gene namesi
Name:Gcfc2
Synonyms:Gcf, Tcf9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2141656. Gcfc2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 769769GC-rich sequence DNA-binding factor 2PRO_0000262647Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei16 – 161PhosphoserineBy similarity
Modified residuei17 – 171PhosphoserineBy similarity
Modified residuei19 – 191PhosphoserineBy similarity
Modified residuei85 – 851PhosphoserineBy similarity
Modified residuei86 – 861PhosphothreonineCombined sources
Modified residuei118 – 1181PhosphoserineCombined sources
Modified residuei203 – 2031PhosphoserineBy similarity
Modified residuei206 – 2061PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8BKT3.
MaxQBiQ8BKT3.
PaxDbiQ8BKT3.
PRIDEiQ8BKT3.

PTM databases

iPTMnetiQ8BKT3.
PhosphoSiteiQ8BKT3.

Expressioni

Gene expression databases

BgeeiQ8BKT3.
CleanExiMM_AW146020.
ExpressionAtlasiQ8BKT3. baseline and differential.
GenevisibleiQ8BKT3. MM.

Interactioni

Subunit structurei

Found in the Intron Large (IL) complex, a post-mRNA release spliceosomal complex containing the excised intron, U2, U5 and U6 snRNPs, and splicing factors. Interacts with TFIP11 and DHX15 (By similarity).By similarity

Protein-protein interaction databases

BioGridi236949. 3 interactions.
STRINGi10090.ENSMUSP00000035644.

Structurei

3D structure databases

ProteinModelPortaliQ8BKT3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili256 – 30853Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the GCF family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2136. Eukaryota.
ENOG410YU43. LUCA.
GeneTreeiENSGT00390000000455.
HOGENOMiHOG000112699.
HOVERGENiHBG101878.
InParanoidiQ8BKT3.
KOiK09061.
OMAiQDTWEQQ.
OrthoDBiEOG79KPGP.
PhylomeDBiQ8BKT3.
TreeFamiTF315109.

Family and domain databases

InterProiIPR012890. GCFC.
IPR022783. GCFC_dom.
[Graphical view]
PANTHERiPTHR12214. PTHR12214. 1 hit.
PfamiPF07842. GCFC. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BKT3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALRPQRTFR RRQVESSDSD SDSDGAKEQS AEEPASAGGR TEGAERPRGA
60 70 80 90 100
RSARGRGRVW ASSRRSPGAA PRGDGGAECR TAELSTDEEE GTHTLTGSKG
110 120 130 140 150
DRSPSSDSSC SLEERDVSPI VEIPDAAFIQ AARRKRELAR TPGDYISLDV
160 170 180 190 200
NHSCSTSDCK RSNEEDPESD PDDHEKRILF TPKPQTLRQR MAEETSIRSE
210 220 230 240 250
ESSEESQEDE NQDIWEQQQM RKAVRIPAGQ NTDLSHSSKS QTLKKFDTSI
260 270 280 290 300
SFPPVNLEII KKQLNNRLTL LQESHRSHQR EYEKYEQDIK SSKTAIQNLE
310 320 330 340 350
SASDHAQNYR FYRGMKSYVE NIIDCLNEKI VSIVELESSM YTLLLKRSEA
360 370 380 390 400
LLKRRQDELK CESSYLQQLS RKDETSANGS LAVDEKDQRI LEEIEARRMQ
410 420 430 440 450
RRQARELSGS CDHQEGMSSD DELSPAEMTN FHKCQGDILQ DCKKVFEDVH
460 470 480 490 500
DDFCNVQNIL LKFQQWREKF PDSYYEAFVG FCLPKLLSPL IRVQLLDWNP
510 520 530 540 550
LKMDSIGLDK MPWFTAITEF MESSMDDIGK EDGSDKKILA AVINKTVVPR
560 570 580 590 600
LTDFVETIWD PLSTSQTRSL TVHCRVAFEQ FASENEVSKN KQDLLKSIVA
610 620 630 640 650
RMKKSIEDDI FIPLYPKSSE EGKMSPHSKF QERQFWGALK LFRNILLWNG
660 670 680 690 700
LLPDDTLQDL GLGKLLNRYL IISLTNAVPG PDVVKKCSQI AACLPERWFE
710 720 730 740 750
NSAMRTSIPQ LENFIKFLLQ SAQKLSSSEF RNEVSEIILI LVKVKALTQA
760
ESLREERPLE PLPAQSTGV
Length:769
Mass (Da):87,442
Last modified:November 28, 2006 - v2
Checksum:iFCD6573BFE1C0DB7
GO
Isoform 2 (identifier: Q8BKT3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     399-405: MQRRQAR → YLYKVQT
     406-769: Missing.

Note: No experimental confirmation available.
Show »
Length:405
Mass (Da):45,927
Checksum:iF324386925D9BDF0
GO

Sequence cautioni

The sequence BAC34411.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti121 – 1211V → F in BAE22958 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei399 – 4057MQRRQAR → YLYKVQT in isoform 2. 1 PublicationVSP_021799
Alternative sequencei406 – 769364Missing in isoform 2. 1 PublicationVSP_021800Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK050789 mRNA. Translation: BAC34411.1. Different initiation.
AK077263 mRNA. Translation: BAC36717.1.
AK136387 mRNA. Translation: BAE22958.1.
AC129024 Genomic DNA. No translation available.
CCDSiCCDS20260.1. [Q8BKT3-1]
RefSeqiNP_808552.2. NM_177884.3. [Q8BKT3-1]
UniGeneiMm.474487.

Genome annotation databases

EnsembliENSMUST00000043195; ENSMUSP00000035644; ENSMUSG00000035125. [Q8BKT3-1]
GeneIDi330361.
KEGGimmu:330361.
UCSCiuc009clc.1. mouse. [Q8BKT3-2]
uc009cld.1. mouse. [Q8BKT3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK050789 mRNA. Translation: BAC34411.1. Different initiation.
AK077263 mRNA. Translation: BAC36717.1.
AK136387 mRNA. Translation: BAE22958.1.
AC129024 Genomic DNA. No translation available.
CCDSiCCDS20260.1. [Q8BKT3-1]
RefSeqiNP_808552.2. NM_177884.3. [Q8BKT3-1]
UniGeneiMm.474487.

3D structure databases

ProteinModelPortaliQ8BKT3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi236949. 3 interactions.
STRINGi10090.ENSMUSP00000035644.

PTM databases

iPTMnetiQ8BKT3.
PhosphoSiteiQ8BKT3.

Proteomic databases

EPDiQ8BKT3.
MaxQBiQ8BKT3.
PaxDbiQ8BKT3.
PRIDEiQ8BKT3.

Protocols and materials databases

DNASUi330361.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043195; ENSMUSP00000035644; ENSMUSG00000035125. [Q8BKT3-1]
GeneIDi330361.
KEGGimmu:330361.
UCSCiuc009clc.1. mouse. [Q8BKT3-2]
uc009cld.1. mouse. [Q8BKT3-1]

Organism-specific databases

CTDi6936.
MGIiMGI:2141656. Gcfc2.

Phylogenomic databases

eggNOGiKOG2136. Eukaryota.
ENOG410YU43. LUCA.
GeneTreeiENSGT00390000000455.
HOGENOMiHOG000112699.
HOVERGENiHBG101878.
InParanoidiQ8BKT3.
KOiK09061.
OMAiQDTWEQQ.
OrthoDBiEOG79KPGP.
PhylomeDBiQ8BKT3.
TreeFamiTF315109.

Miscellaneous databases

ChiTaRSiGcfc2. mouse.
NextBioi399313.
PROiQ8BKT3.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BKT3.
CleanExiMM_AW146020.
ExpressionAtlasiQ8BKT3. baseline and differential.
GenevisibleiQ8BKT3. MM.

Family and domain databases

InterProiIPR012890. GCFC.
IPR022783. GCFC_dom.
[Graphical view]
PANTHERiPTHR12214. PTHR12214. 1 hit.
PfamiPF07842. GCFC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 8-769 (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Embryo, Lung, Ovary and Uterus.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-118, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-86, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney and Testis.

Entry informationi

Entry nameiGCFC2_MOUSE
AccessioniPrimary (citable) accession number: Q8BKT3
Secondary accession number(s): Q3UWF7, Q8BK44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 28, 2006
Last modified: March 16, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.