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Protein

Transcription factor HES-7

Gene

Hes7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional repressor. Represses transcription from both N box- and E box-containing promoters. May with HES1, cooperatively regulate somite formation in the presomitic mesoderm (PSM). May function as a segmentation clock, which is essential for coordinated somite segmentation.3 Publications

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • transcription factor binding Source: UniProtKB

GO - Biological processi

  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • Notch signaling pathway Source: MGI
  • post-anal tail morphogenesis Source: MGI
  • regulation of somitogenesis Source: MGI
  • rhythmic process Source: MGI
  • skeletal system development Source: MGI
  • somitogenesis Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor HES-7
Short name:
mHes7
Alternative name(s):
Hairy and enhancer of split 7
bHLH factor Hes7
Gene namesi
Name:Hes7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2135679. Hes7.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi14 – 141K → R: No loss of repressor activity, somite segmentation disrupted at later stages. 1 Publication
Mutagenesisi17 – 171K → R: Loss of repressor activity. 1 Publication
Mutagenesisi22 – 221K → R: Loss of repressor activity. 1 Publication
Mutagenesisi25 – 251R → W: Loss of repressor activity. 1 Publication
Mutagenesisi52 – 521K → R: Loss of repressor activity. 1 Publication
Mutagenesisi55 – 551K → R: Loss of repressor activity. 1 Publication
Mutagenesisi58 – 581I → V: No loss of repressor activity. 1 Publication
Mutagenesisi129 – 1291K → R: No loss of repressor activity. 1 Publication
Mutagenesisi186 – 1861D → Y: Partial loss of repressor activity. 1 Publication
Mutagenesisi211 – 2111K → R: No loss of repressor activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 225225Transcription factor HES-7PRO_0000252423Add
BLAST

Proteomic databases

PaxDbiQ8BKT2.
PRIDEiQ8BKT2.

PTM databases

iPTMnetiQ8BKT2.
PhosphoSiteiQ8BKT2.

Expressioni

Developmental stagei

Dynamic expression in the presomitic mesoderm (PSM). At E8.5, expressed in two bilateral domains: rostral and caudal stripes. The rostral bands are located posterior to the newly formed somite, while the caudal bands extend to the most caudal tip. During E9.0-E12.0 stages, again specifically expressed in two domains of the PSM.2 Publications

Inductioni

By Notch-signaling.1 Publication

Gene expression databases

BgeeiQ8BKT2.
CleanExiMM_HES7.
GenevisibleiQ8BKT2. MM.

Interactioni

Subunit structurei

Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family.By similarity

GO - Molecular functioni

  • transcription factor binding Source: UniProtKB

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000024543.

Structurei

3D structure databases

ProteinModelPortaliQ8BKT2.
SMRiQ8BKT2. Positions 14-73.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 6958bHLHPROSITE-ProRule annotationAdd
BLAST
Domaini92 – 12231OrangePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi221 – 2244WRPW motif

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi128 – 22598Pro-richAdd
BLAST

Domaini

Has a particular type of basic domain which includes a helix-interrupting proline.
The C-terminal WRPW motif is a transcriptional repression motif which is necessary for interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.By similarity

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation
Contains 1 Orange domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4304. Eukaryota.
ENOG4111F0X. LUCA.
GeneTreeiENSGT00730000111282.
HOGENOMiHOG000236346.
HOVERGENiHBG096052.
InParanoidiQ8BKT2.
KOiK09087.
OMAiILGPALH.
OrthoDBiEOG77DJ80.
PhylomeDBiQ8BKT2.
TreeFamiTF351373.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR032644. HES-7.
IPR003650. Orange_dom.
[Graphical view]
PANTHERiPTHR10985:SF84. PTHR10985:SF84. 1 hit.
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
PS51054. ORANGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BKT2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTRERAENR DGPKMLKPLV EKRRRDRINR SLEELRLLLL ERTRDQNLRN
60 70 80 90 100
PKLEKAEILE FAVGYLRERS RVEPPGVPRS PGQDAEALAS CYLSGFRECL
110 120 130 140 150
LRLAAFAHDA SPAARSQLFS ALHGYRRPKP PRPEAVDPGL PAPRPPLDPA
160 170 180 190 200
SPILGPALHQ RPPVHQGPPS PRLAWSPSHC SSRAGDSGAP APLTGLLPPP
210 220
PPPYRQDGAP KAPSLPPPAF WRPWP
Length:225
Mass (Da):24,890
Last modified:March 1, 2003 - v1
Checksum:i4B22E7D71A7FF5E8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti214 – 2141S → P in BAB39526 (PubMed:11260262).Curated
Sequence conflicti214 – 2141S → P in BAB41134 (PubMed:11260262).Curated
Sequence conflicti214 – 2141S → P in AAI34379 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB049065 mRNA. Translation: BAB39526.1.
AB050104 Genomic DNA. Translation: BAB41134.1.
AK050791 mRNA. Translation: BAC34412.1.
AL645527 Genomic DNA. Translation: CAI35247.1.
BC134378 mRNA. Translation: AAI34379.1.
CCDSiCCDS24883.1.
RefSeqiNP_149030.2. NM_033041.4.
UniGeneiMm.98505.

Genome annotation databases

EnsembliENSMUST00000024543; ENSMUSP00000024543; ENSMUSG00000023781.
GeneIDi84653.
KEGGimmu:84653.
UCSCiuc007jpi.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB049065 mRNA. Translation: BAB39526.1.
AB050104 Genomic DNA. Translation: BAB41134.1.
AK050791 mRNA. Translation: BAC34412.1.
AL645527 Genomic DNA. Translation: CAI35247.1.
BC134378 mRNA. Translation: AAI34379.1.
CCDSiCCDS24883.1.
RefSeqiNP_149030.2. NM_033041.4.
UniGeneiMm.98505.

3D structure databases

ProteinModelPortaliQ8BKT2.
SMRiQ8BKT2. Positions 14-73.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000024543.

PTM databases

iPTMnetiQ8BKT2.
PhosphoSiteiQ8BKT2.

Proteomic databases

PaxDbiQ8BKT2.
PRIDEiQ8BKT2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000024543; ENSMUSP00000024543; ENSMUSG00000023781.
GeneIDi84653.
KEGGimmu:84653.
UCSCiuc007jpi.1. mouse.

Organism-specific databases

CTDi84667.
MGIiMGI:2135679. Hes7.

Phylogenomic databases

eggNOGiKOG4304. Eukaryota.
ENOG4111F0X. LUCA.
GeneTreeiENSGT00730000111282.
HOGENOMiHOG000236346.
HOVERGENiHBG096052.
InParanoidiQ8BKT2.
KOiK09087.
OMAiILGPALH.
OrthoDBiEOG77DJ80.
PhylomeDBiQ8BKT2.
TreeFamiTF351373.

Miscellaneous databases

PROiQ8BKT2.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BKT2.
CleanExiMM_HES7.
GenevisibleiQ8BKT2. MM.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR032644. HES-7.
IPR003650. Orange_dom.
[Graphical view]
PANTHERiPTHR10985:SF84. PTHR10985:SF84. 1 hit.
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
PS51054. ORANGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Hes7: a bHLH-type repressor gene regulated by Notch and expressed in the presomitic mesoderm."
    Bessho Y., Miyoshi G., Sakata R., Kageyama R.
    Genes Cells 6:175-185(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, DEVELOPMENTAL STAGE, INDUCTION.
    Tissue: Embryo.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Instability of Hes7 protein is crucial for the somite segmentation clock."
    Hirata H., Bessho Y., Kokubu H., Masamizu Y., Yamada S., Lewis J., Kageyama R.
    Nat. Genet. 36:750-754(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE, MUTAGENESIS OF LYS-14; LYS-17; LYS-22; LYS-52; LYS-55; LYS-129 AND LYS-211.
  6. "Mutation of Hairy-and-Enhancer-of-Split-7 in humans causes spondylocostal dysostosis."
    Sparrow D.B., Guillen-Navarro E., Fatkin D., Dunwoodie S.L.
    Hum. Mol. Genet. 17:3761-3766(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF ARG-25.
  7. "Two novel missense mutations in HAIRY-AND-ENHANCER-OF-SPLIT-7 in a family with spondylocostal dysostosis."
    Sparrow D.B., Sillence D., Wouters M.A., Turnpenny P.D., Dunwoodie S.L.
    Eur. J. Hum. Genet. 18:674-679(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF ILE-58 AND ASP-186.

Entry informationi

Entry nameiHES7_MOUSE
AccessioniPrimary (citable) accession number: Q8BKT2
Secondary accession number(s): A3KPD3, Q99JA6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: March 1, 2003
Last modified: June 8, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.