Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Transcription factor HES-7

Gene

Hes7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional repressor. Represses transcription from both N box- and E box-containing promoters. May with HES1, cooperatively regulate somite formation in the presomitic mesoderm (PSM). May function as a segmentation clock, which is essential for coordinated somite segmentation.3 Publications

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • transcription factor binding Source: UniProtKB

GO - Biological processi

  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • Notch signaling pathway Source: MGI
  • post-anal tail morphogenesis Source: MGI
  • regulation of somitogenesis Source: MGI
  • rhythmic process Source: MGI
  • skeletal system development Source: MGI
  • somitogenesis Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor HES-7
Short name:
mHes7
Alternative name(s):
Hairy and enhancer of split 7
bHLH factor Hes7
Gene namesi
Name:Hes7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2135679. Hes7.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi14K → R: No loss of repressor activity, somite segmentation disrupted at later stages. 1 Publication1
Mutagenesisi17K → R: Loss of repressor activity. 1 Publication1
Mutagenesisi22K → R: Loss of repressor activity. 1 Publication1
Mutagenesisi25R → W: Loss of repressor activity. 1 Publication1
Mutagenesisi52K → R: Loss of repressor activity. 1 Publication1
Mutagenesisi55K → R: Loss of repressor activity. 1 Publication1
Mutagenesisi58I → V: No loss of repressor activity. 1 Publication1
Mutagenesisi129K → R: No loss of repressor activity. 1 Publication1
Mutagenesisi186D → Y: Partial loss of repressor activity. 1 Publication1
Mutagenesisi211K → R: No loss of repressor activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002524231 – 225Transcription factor HES-7Add BLAST225

Proteomic databases

PaxDbiQ8BKT2.
PRIDEiQ8BKT2.

PTM databases

iPTMnetiQ8BKT2.
PhosphoSitePlusiQ8BKT2.

Expressioni

Developmental stagei

Dynamic expression in the presomitic mesoderm (PSM). At E8.5, expressed in two bilateral domains: rostral and caudal stripes. The rostral bands are located posterior to the newly formed somite, while the caudal bands extend to the most caudal tip. During E9.0-E12.0 stages, again specifically expressed in two domains of the PSM.2 Publications

Inductioni

By Notch-signaling.1 Publication

Gene expression databases

BgeeiENSMUSG00000023781.
CleanExiMM_HES7.
GenevisibleiQ8BKT2. MM.

Interactioni

Subunit structurei

Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family.By similarity

GO - Molecular functioni

  • transcription factor binding Source: UniProtKB

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000024543.

Structurei

3D structure databases

ProteinModelPortaliQ8BKT2.
SMRiQ8BKT2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 69bHLHPROSITE-ProRule annotationAdd BLAST58
Domaini92 – 122OrangePROSITE-ProRule annotationAdd BLAST31

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi221 – 224WRPW motif4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi128 – 225Pro-richAdd BLAST98

Domaini

Has a particular type of basic domain which includes a helix-interrupting proline.
The C-terminal WRPW motif is a transcriptional repression motif which is necessary for interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.By similarity

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation
Contains 1 Orange domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4304. Eukaryota.
ENOG4111F0X. LUCA.
GeneTreeiENSGT00730000111282.
HOGENOMiHOG000236346.
HOVERGENiHBG096052.
InParanoidiQ8BKT2.
KOiK09087.
OMAiILGPALH.
OrthoDBiEOG091G0XFV.
PhylomeDBiQ8BKT2.
TreeFamiTF351373.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR032644. HES-7.
IPR003650. Orange_dom.
[Graphical view]
PANTHERiPTHR10985:SF84. PTHR10985:SF84. 1 hit.
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
PS51054. ORANGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BKT2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTRERAENR DGPKMLKPLV EKRRRDRINR SLEELRLLLL ERTRDQNLRN
60 70 80 90 100
PKLEKAEILE FAVGYLRERS RVEPPGVPRS PGQDAEALAS CYLSGFRECL
110 120 130 140 150
LRLAAFAHDA SPAARSQLFS ALHGYRRPKP PRPEAVDPGL PAPRPPLDPA
160 170 180 190 200
SPILGPALHQ RPPVHQGPPS PRLAWSPSHC SSRAGDSGAP APLTGLLPPP
210 220
PPPYRQDGAP KAPSLPPPAF WRPWP
Length:225
Mass (Da):24,890
Last modified:March 1, 2003 - v1
Checksum:i4B22E7D71A7FF5E8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti214S → P in BAB39526 (PubMed:11260262).Curated1
Sequence conflicti214S → P in BAB41134 (PubMed:11260262).Curated1
Sequence conflicti214S → P in AAI34379 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB049065 mRNA. Translation: BAB39526.1.
AB050104 Genomic DNA. Translation: BAB41134.1.
AK050791 mRNA. Translation: BAC34412.1.
AL645527 Genomic DNA. Translation: CAI35247.1.
BC134378 mRNA. Translation: AAI34379.1.
CCDSiCCDS24883.1.
RefSeqiNP_149030.2. NM_033041.4.
UniGeneiMm.98505.

Genome annotation databases

EnsembliENSMUST00000024543; ENSMUSP00000024543; ENSMUSG00000023781.
GeneIDi84653.
KEGGimmu:84653.
UCSCiuc007jpi.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB049065 mRNA. Translation: BAB39526.1.
AB050104 Genomic DNA. Translation: BAB41134.1.
AK050791 mRNA. Translation: BAC34412.1.
AL645527 Genomic DNA. Translation: CAI35247.1.
BC134378 mRNA. Translation: AAI34379.1.
CCDSiCCDS24883.1.
RefSeqiNP_149030.2. NM_033041.4.
UniGeneiMm.98505.

3D structure databases

ProteinModelPortaliQ8BKT2.
SMRiQ8BKT2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000024543.

PTM databases

iPTMnetiQ8BKT2.
PhosphoSitePlusiQ8BKT2.

Proteomic databases

PaxDbiQ8BKT2.
PRIDEiQ8BKT2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000024543; ENSMUSP00000024543; ENSMUSG00000023781.
GeneIDi84653.
KEGGimmu:84653.
UCSCiuc007jpi.1. mouse.

Organism-specific databases

CTDi84667.
MGIiMGI:2135679. Hes7.

Phylogenomic databases

eggNOGiKOG4304. Eukaryota.
ENOG4111F0X. LUCA.
GeneTreeiENSGT00730000111282.
HOGENOMiHOG000236346.
HOVERGENiHBG096052.
InParanoidiQ8BKT2.
KOiK09087.
OMAiILGPALH.
OrthoDBiEOG091G0XFV.
PhylomeDBiQ8BKT2.
TreeFamiTF351373.

Miscellaneous databases

PROiQ8BKT2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000023781.
CleanExiMM_HES7.
GenevisibleiQ8BKT2. MM.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR032644. HES-7.
IPR003650. Orange_dom.
[Graphical view]
PANTHERiPTHR10985:SF84. PTHR10985:SF84. 1 hit.
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
PS51054. ORANGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHES7_MOUSE
AccessioniPrimary (citable) accession number: Q8BKT2
Secondary accession number(s): A3KPD3, Q99JA6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.