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Protein

Pumilio homolog 3

Gene

Pum3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits the poly(ADP-ribosyl)ation activity of PARP1 and the degradation of PARP1 by CASP3 following genotoxic stress. Binds to double-stranded RNA or DNA without sequence specificity. Involved in development of the eye and of primordial germ cells.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

DNA-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Pumilio homolog 3Curated
Gene namesi
Name:Pum3Imported
Synonyms:D19Bwg1357eImported, Kiaa0020Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:106253. Pum3.

Subcellular locationi

  • Nucleusnucleolus By similarity
  • Nucleusnucleoplasm By similarity
  • Chromosome By similarity

  • Note: Localizes predominantly in the nucleolus with minor punctate signals in the nucleoplasm.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 647647Pumilio homolog 3PRO_0000075930Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei33 – 331N6-acetyllysineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ8BKS9.
MaxQBiQ8BKS9.
PaxDbiQ8BKS9.
PeptideAtlasiQ8BKS9.
PRIDEiQ8BKS9.

PTM databases

iPTMnetiQ8BKS9.
PhosphoSiteiQ8BKS9.

Expressioni

Tissue specificityi

In the adult eye, expressed primarily in retinal ganglion cells and, to a lesser extent, in the pigmented cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000041360.

Interactioni

Subunit structurei

Interacts with PARP1 (via catalytic domain).By similarity

Protein-protein interaction databases

BioGridi206863. 2 interactions.
IntActiQ8BKS9. 1 interaction.
MINTiMINT-1844030.
STRINGi10090.ENSMUSP00000075573.

Structurei

3D structure databases

ProteinModelPortaliQ8BKS9.
SMRiQ8BKS9. Positions 130-645.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini142 – 509368PUM-HDPROSITE-ProRule annotationAdd
BLAST
Repeati176 – 21136Pumilio 1By similarityAdd
BLAST
Repeati212 – 24736Pumilio 2By similarityAdd
BLAST
Repeati248 – 27629Pumilio 3By similarityAdd
BLAST
Repeati288 – 32437Pumilio 4By similarityAdd
BLAST
Repeati325 – 36036Pumilio 5By similarityAdd
BLAST
Repeati361 – 39636Pumilio 6By similarityAdd
BLAST
Repeati397 – 43438Pumilio 7By similarityAdd
BLAST
Repeati435 – 50369Pumilio 8By similarityAdd
BLAST
Repeati504 – 55047Pumilio 9By similarityAdd
BLAST
Repeati551 – 59545Pumilio 10By similarityAdd
BLAST
Repeati596 – 63540Pumilio 11By similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi105 – 11713Nuclear localization signalBy similarityAdd
BLAST

Domaini

A 90 degree bend between Pumilio repeats 3 and 4 gives rise to a L-shaped protein.By similarity

Sequence similaritiesi

Contains 1 PUM-HD domain.PROSITE-ProRule annotation
Contains 11 pumilio repeats.By similarity

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2050. Eukaryota.
ENOG410XSHY. LUCA.
HOGENOMiHOG000237322.
HOVERGENiHBG052166.
InParanoidiQ8BKS9.
KOiK14844.
OMAiYLQEHAQ.
PhylomeDBiQ8BKS9.
TreeFamiTF312954.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR012959. CPL_dom.
IPR033133. PUM-HD.
IPR001313. Pumilio_RNA-bd_rpt.
[Graphical view]
PfamiPF08144. CPL. 1 hit.
[Graphical view]
SMARTiSM00025. Pumilio. 6 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
PROSITEiPS50302. PUM. 6 hits.
PS50303. PUM_HD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BKS9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVKGKKKFT GKSPQTSQGK NKFHKNSESS SSKTFPRKAV KEGGPKVTSK
60 70 80 90 100
NFEKGATKPG KKGVKQFKNK PQGGKGPKDK FQKANKFSKK RKFQPDGESD
110 120 130 140 150
ESGAKKPKWD DFKKKKKELK QSRQLSDKTN YDIVVRAKHI WESLRRKDCD
160 170 180 190 200
KEKRVKLMSD LQKLIQGKIK TIAFAHDSTR VIQCFIQYGN EEQRKQAFQE
210 220 230 240 250
LQGDLVELSK AKYSRNIVKK FLMYGSKPQV AEIIRSFKGH VRKMLRHSEA
260 270 280 290 300
SAIVEYAYND KAILEQRNML TEELYGNTFQ LYKSADHPTL DKVLELQPAK
310 320 330 340 350
LELIMDEMKQ ILTPMAQKEA VIKHSLVHKV FLDFFTYAPP KPRSELIEAI
360 370 380 390 400
REAVVYLAHT HDGARVAMHC LWHGTPKDRK VIVKTMKTYV EKVANGQYSH
410 420 430 440 450
LVLLAAFDCI DDTKLVKQII ISEIISSLPS IVNDKYGRKV LLYLMSPRDP
460 470 480 490 500
AHTVPELIEL LQKGDGNAHS KKDTAIRRRE LLESISPALL SYLQGHTQEV
510 520 530 540 550
VLDKSACVLV SDMLGSATGD VQPAMDAIAS LAAAELHPGG KDGELHVAEH
560 570 580 590 600
PAGHLVLKWL LEQDKKMKES GKEGCFAKTL VERVGMKNLK SWASINRGAI
610 620 630 640
ILSSLLQSCD QEVVNKVKGG LKPLIPTLEK NKSSSRGIQT LLEKLTA
Length:647
Mass (Da):72,800
Last modified:November 8, 2005 - v2
Checksum:i193533640A3AFE50
GO

Sequence cautioni

The sequence BAC34427 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAC40135 differs from that shown. Reason: Frameshift at positions 79 and 632. Curated
The sequence BAE24873 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK050829 mRNA. Translation: BAC34427.1. Different initiation.
AK088081 mRNA. Translation: BAC40135.1. Frameshift.
AK141893 mRNA. Translation: BAE24873.1. Different initiation.
AK172872 mRNA. Translation: BAD32150.1.
RefSeqiNP_803425.1. NM_177474.5.
XP_006527246.1. XM_006527183.2.
UniGeneiMm.261027.

Genome annotation databases

GeneIDi52874.
KEGGimmu:52874.
UCSCiuc008hby.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK050829 mRNA. Translation: BAC34427.1. Different initiation.
AK088081 mRNA. Translation: BAC40135.1. Frameshift.
AK141893 mRNA. Translation: BAE24873.1. Different initiation.
AK172872 mRNA. Translation: BAD32150.1.
RefSeqiNP_803425.1. NM_177474.5.
XP_006527246.1. XM_006527183.2.
UniGeneiMm.261027.

3D structure databases

ProteinModelPortaliQ8BKS9.
SMRiQ8BKS9. Positions 130-645.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi206863. 2 interactions.
IntActiQ8BKS9. 1 interaction.
MINTiMINT-1844030.
STRINGi10090.ENSMUSP00000075573.

PTM databases

iPTMnetiQ8BKS9.
PhosphoSiteiQ8BKS9.

Proteomic databases

EPDiQ8BKS9.
MaxQBiQ8BKS9.
PaxDbiQ8BKS9.
PeptideAtlasiQ8BKS9.
PRIDEiQ8BKS9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi52874.
KEGGimmu:52874.
UCSCiuc008hby.1. mouse.

Organism-specific databases

CTDi9933.
MGIiMGI:106253. Pum3.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG2050. Eukaryota.
ENOG410XSHY. LUCA.
HOGENOMiHOG000237322.
HOVERGENiHBG052166.
InParanoidiQ8BKS9.
KOiK14844.
OMAiYLQEHAQ.
PhylomeDBiQ8BKS9.
TreeFamiTF312954.

Miscellaneous databases

PROiQ8BKS9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000041360.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR012959. CPL_dom.
IPR033133. PUM-HD.
IPR001313. Pumilio_RNA-bd_rpt.
[Graphical view]
PfamiPF08144. CPL. 1 hit.
[Graphical view]
SMARTiSM00025. Pumilio. 6 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
PROSITEiPS50302. PUM. 6 hits.
PS50303. PUM_HD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPUM3_MOUSE
AccessioniPrimary (citable) accession number: Q8BKS9
Secondary accession number(s): Q6A0E6, Q8BU15
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: September 7, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.