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Protein

Heparan sulfate glucosamine 3-O-sulfotransferase 3A1

Gene

Hs3st3a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to an N-unsubstituted glucosamine linked to a 2-O-sulfo iduronic acid unit on heparan sulfate. Catalyzes the O-sulfation of glucosamine in IdoUA2S-GlcNS and also in IdoUA2S-GlcNH2. Unlike 3-OST-1, does not convert non-anticoagulant heparan sulfate to anticoagulant heparan sulfate (By similarity).By similarity

Catalytic activityi

3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei230 – 2301PAPSBy similarity
Binding sitei238 – 2381PAPSBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi149 – 1535PAPSBy similarity
Nucleotide bindingi355 – 3595PAPSBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

ReactomeiR-MMU-2022928. HS-GAG biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Heparan sulfate glucosamine 3-O-sulfotransferase 3A1 (EC:2.8.2.23)
Alternative name(s):
Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 3A1
Short name:
Heparan sulfate 3-O-sulfotransferase 3A1
Gene namesi
Name:Hs3st3a1
Synonyms:3ost3a1, Hs3st3a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1333861. Hs3st3a1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2424CytoplasmicSequence analysisAdd
BLAST
Transmembranei25 – 4319Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini44 – 393350LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 393393Heparan sulfate glucosamine 3-O-sulfotransferase 3A1PRO_0000085218Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi260 – 2601N-linked (GlcNAc...)Sequence analysis
Glycosylationi331 – 3311N-linked (GlcNAc...)Sequence analysis
Disulfide bondi338 ↔ 350By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8BKN6.
PRIDEiQ8BKN6.

PTM databases

iPTMnetiQ8BKN6.
PhosphoSiteiQ8BKN6.

Expressioni

Gene expression databases

BgeeiQ8BKN6.
ExpressionAtlasiQ8BKN6. baseline and differential.
GenevisibleiQ8BKN6. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000055930.

Structurei

3D structure databases

ProteinModelPortaliQ8BKN6.
SMRiQ8BKN6. Positions 126-393.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni171 – 1777Substrate bindingBy similarity
Regioni202 – 2054Substrate bindingBy similarity
Regioni270 – 2712Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the sulfotransferase 1 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3704. Eukaryota.
ENOG410XS59. LUCA.
GeneTreeiENSGT00760000119023.
HOGENOMiHOG000036663.
HOVERGENiHBG053377.
InParanoidiQ8BKN6.
KOiK07809.
OMAiLAMWPAG.
OrthoDBiEOG7PS1GM.
PhylomeDBiQ8BKN6.
TreeFamiTF350755.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8BKN6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPSGPTGAQ PSPAEPLSRS IFRKFLLMLC SLLTSLYVFY CLAERCPPGS
60 70 80 90 100
GPVAGVPGRG VPAGPRELAM WPAGAPRKRL LQLRQRRRRG RSGPGDSSDQ
110 120 130 140 150
EEQSPGLAAA PGGSGAGSSV AEAQPGTLAL LLDEGSKQLP QAIIIGVKKG
160 170 180 190 200
GTRALLEFLR VHPDVRAVGA EPHFFDRSYH KGLAWYRDLM PRTLEGQITM
210 220 230 240 250
EKTPSYFVTR EAPARISAMS KDTKLIVVVR DPVTRAISDY TQTLSKRPDI
260 270 280 290 300
PSFESLTFRN RSAGLIDTSW SAIQIGLYAK HLEPWLRHFP LGQMLFVSGE
310 320 330 340 350
RLVSDPAGEL RRVQDFLGLK RIITDKHFYF NQTKGFPCLK KAEGSGKPHC
360 370 380 390
LGKTKGRAHP TIAREVLRQL RDFYRPFNRK FYQMTGRDFG WDG
Length:393
Mass (Da):43,483
Last modified:March 1, 2003 - v1
Checksum:i0B3EFF6EBDB80412
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK051284 mRNA. Translation: BAC34592.1.
AL646051, AL672141 Genomic DNA. Translation: CAI24639.1.
AL672141, AL646051 Genomic DNA. Translation: CAI24606.1.
CCDSiCCDS24840.1.
RefSeqiNP_849201.1. NM_178870.5.
UniGeneiMm.340214.

Genome annotation databases

EnsembliENSMUST00000058652; ENSMUSP00000055930; ENSMUSG00000047759.
GeneIDi15478.
KEGGimmu:15478.
UCSCiuc007jkr.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK051284 mRNA. Translation: BAC34592.1.
AL646051, AL672141 Genomic DNA. Translation: CAI24639.1.
AL672141, AL646051 Genomic DNA. Translation: CAI24606.1.
CCDSiCCDS24840.1.
RefSeqiNP_849201.1. NM_178870.5.
UniGeneiMm.340214.

3D structure databases

ProteinModelPortaliQ8BKN6.
SMRiQ8BKN6. Positions 126-393.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000055930.

PTM databases

iPTMnetiQ8BKN6.
PhosphoSiteiQ8BKN6.

Proteomic databases

PaxDbiQ8BKN6.
PRIDEiQ8BKN6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000058652; ENSMUSP00000055930; ENSMUSG00000047759.
GeneIDi15478.
KEGGimmu:15478.
UCSCiuc007jkr.1. mouse.

Organism-specific databases

CTDi9955.
MGIiMGI:1333861. Hs3st3a1.

Phylogenomic databases

eggNOGiKOG3704. Eukaryota.
ENOG410XS59. LUCA.
GeneTreeiENSGT00760000119023.
HOGENOMiHOG000036663.
HOVERGENiHBG053377.
InParanoidiQ8BKN6.
KOiK07809.
OMAiLAMWPAG.
OrthoDBiEOG7PS1GM.
PhylomeDBiQ8BKN6.
TreeFamiTF350755.

Enzyme and pathway databases

ReactomeiR-MMU-2022928. HS-GAG biosynthesis.

Miscellaneous databases

PROiQ8BKN6.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BKN6.
ExpressionAtlasiQ8BKN6. baseline and differential.
GenevisibleiQ8BKN6. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Spinal ganglion.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.

Entry informationi

Entry nameiHS3SA_MOUSE
AccessioniPrimary (citable) accession number: Q8BKN6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 1, 2003
Last modified: June 8, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.