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Protein

Trinucleotide repeat-containing gene 6B protein

Gene

Tnrc6b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent translational repression and siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins As scaffoldng protein associates with argonaute proteins bound to partially complementary mRNAs and simultaneously can recruit CCR4-NOT and PAN deadenylase complexes (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

RNA-mediated gene silencing, Translation regulation

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-426496. Post-transcriptional silencing by small RNAs.

Names & Taxonomyi

Protein namesi
Recommended name:
Trinucleotide repeat-containing gene 6B protein
Gene namesi
Name:Tnrc6b
Synonyms:Kiaa1093
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:2443730. Tnrc6b.

Subcellular locationi

  • CytoplasmP-body By similarity

  • Note: Mammalian P-bodies are also known as GW bodies (GWBs).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 18101810Trinucleotide repeat-containing gene 6B proteinPRO_0000373981Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei913 – 9131PhosphoserineBy similarity
Modified residuei1409 – 14091PhosphoserineCombined sources
Modified residuei1426 – 14261PhosphothreonineCombined sources
Modified residuei1438 – 14381PhosphoserineCombined sources
Modified residuei1441 – 14411PhosphothreonineCombined sources
Modified residuei1793 – 17931PhosphoserineBy similarity
Modified residuei1809 – 18091PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8BKI2.
MaxQBiQ8BKI2.
PaxDbiQ8BKI2.
PRIDEiQ8BKI2.

PTM databases

iPTMnetiQ8BKI2.
PhosphoSiteiQ8BKI2.

Expressioni

Gene expression databases

BgeeiQ8BKI2.
GenevisibleiQ8BKI2. MM.

Interactioni

Subunit structurei

Interacts with AGO1, AGO2, AGO3 and AGO4. Interacts with CNOT1; the interaction mediates the association with the CCR4-NOT complex. Interacts with PAN3; the interaction mediates the association with the PAN complex (By similarity).By similarity

Protein-protein interaction databases

BioGridi229484. 1 interaction.
DIPiDIP-48568N.
IntActiQ8BKI2. 1 interaction.
STRINGi10090.ENSMUSP00000064336.

Structurei

3D structure databases

ProteinModelPortaliQ8BKI2.
SMRiQ8BKI2. Positions 1624-1704.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1625 – 169773RRMAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni37 – 1028992Interaction with argonaute proteinsBy similarityAdd
BLAST
Regioni1191 – 1700510Silencing domain; interaction with CNOT1 and PAN3By similarityAdd
BLAST
Regioni1449 – 146719PABPC1-interacting motif-2 (PAM2)By similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili33 – 7543Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the GW182 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IET2. Eukaryota.
ENOG410YDKH. LUCA.
GeneTreeiENSGT00410000025966.
HOGENOMiHOG000049171.
InParanoidiQ8BKI2.
KOiK18412.
OMAiMPRPEGK.
OrthoDBiEOG7353VZ.
PhylomeDBiQ8BKI2.
TreeFamiTF329702.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR019486. Argonaute_hook_dom.
IPR012677. Nucleotide-bd_a/b_plait.
IPR032226. TNRC6_PABC-bd.
[Graphical view]
PfamiPF10427. Ago_hook. 1 hit.
PF16608. TNRC6-PABC_bdg. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BKI2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQTNEGEVEE ESSSQVEQED FVMEGHGKTP PPGEESKQEK EQEREEQLME
60 70 80 90 100
DKKRKKEDKK KKEATQKVTE QKTKVPEVTK PSLSQPTAAS PIGSSPSPPV
110 120 130 140 150
NGGNNAKRVA VPNGQPPSAA RYMPREVPPR FRCQQDHKVL LKRGQPPPPS
160 170 180 190 200
CMLLGGGAGP PPCTAPGANP NNNAQVTGAL LQSESGTAPE STLGGAAASN
210 220 230 240 250
YANSTWGPGA SSNSGASPNP IHIWDKVIVD GSDMEEWPCI ASKDTESSSE
260 270 280 290 300
NTTDNNSASN PGSEKSSLPG STTSNKGKGS QCQAASSGNE CNLGVWKSDP
310 320 330 340 350
KAKSVQPPNS TSDSNNGLGN WRSTSGQDRI GPGSGFSNFN PNSNPSAWPA
360 370 380 390 400
LVQEGTSRKG ALETESSSSS AQVSTVGQAS REQQSKMENA GVNFVVSGRE
410 420 430 440 450
QAQIHNTDGP KNGNTNSLNL SSPNPMENKG MPFGMGLGNT SRSTDAPSQS
460 470 480 490 500
TGDRKTGSVG SWGAARGPSG TDTVSGQSNS GNNGNNGKDR EDSWKGASVP
510 520 530 540 550
KPTGSKSDSW DNNNRSTGGS WNFGPQDNND NKWGEGNKMT SGVSQGEWKQ
560 570 580 590 600
PTGSDELKIG EWSGPNQPNS STGAWDNQKG HPLPENQGNA QAPCWGRSSS
610 620 630 640 650
SAGSEVGGQS TGSNHKAGSS DSHNSGRRSY RPTHPDCQAV LQTLLSRTDL
660 670 680 690 700
DPRVLSNTGW GQTQIKQDTV WDIEEVPRPE GKSDKGTEGW ESAATQTKNS
710 720 730 740 750
GGWGDAPSQS NQMKSGWGEL SASTEWKDPK STGGWNDYKN NNSSNWGGGR
760 770 780 790 800
ADEKTPSSWN ESSCKDQGWG GGRQPNQGWT SGKNGWGEEV DQVKNNNWES
810 820 830 840 850
SANKPVSGWG EGGQNEIGTW GNGGNTNLAS KGGWEDCKRS PAWNETGRQP
860 870 880 890 900
NSWNKQHQQQ QQPPPPQPEA SGSWGGPPPP PQGNVRPSNS NWSSGPQPTT
910 920 930 940 950
PKDDEPSGWE EPSPQSISRK MDIDDGTSAW GDPNSYNYKN VNLWDKNSQG
960 970 980 990 1000
GPAPREPNLP TPMTGKSASV WSKSTPPAPD NGTSAWGEPN ESSPGWGEMD
1010 1020 1030 1040 1050
DAGASTTGWG NTPANAPNAM KPNSKSMQDG WGESDGPVTG ARHPSWEEED
1060 1070 1080 1090 1100
DGGVWNTAGS QGSTSSHNSA SWGQGGKKQM KCSLKGGNND SWMNPLAKQF
1110 1120 1130 1140 1150
SNMGLLSQTE DNPSSKMDLS VDKKFDVDKR TMNLGDFNDI MRKDRPGFRP
1160 1170 1180 1190 1200
PNSKDLGTTD SGPYFEKGGS HGLFGNSTAQ SRGLHTPVQP LSSSPGLRAQ
1210 1220 1230 1240 1250
VPPQFISPQV SASMLKQFPN SGLNPGLFNV GPQLSPQQIA MLSQLPQIPQ
1260 1270 1280 1290 1300
FQLACQLLLQ QQQQQQQLLQ NQRKISQAVR QQQEQQLARM VSALQQQQQQ
1310 1320 1330 1340 1350
QQQQQRQPSM KHSPSHPVGP KPHLDNMVPN ALNVGLPDLP TKGPIPGYGS
1360 1370 1380 1390 1400
GFSSGGMDYG MVGGKEAGTE SRFKQWTSMM EGLPSVATQE ATMHKNGAIV
1410 1420 1430 1440 1450
APGKTRGGSP YNQFDIIPGD TLGGHTGPAG DSWLPAKSPP TNKIGSKSSN
1460 1470 1480 1490 1500
ASWPPEFQPG VPWKGIQNID PESDPYVTPG SVLGGTTTSP IVDTDHQLLR
1510 1520 1530 1540 1550
DNTTGSNSSL NTSLPSPGAW PYSASDNSFT NVHSTSAKFP DYKSTWSPDP
1560 1570 1580 1590 1600
IGHNPTHLSN KMWKNHISSR NTTPLTRPPP GLTNPKPASP WSSTAPRSVR
1610 1620 1630 1640 1650
GWGTQDSRIA SASTWSDGGS VRPSYWLVLH NLTPQIDGST LRTICMQHGP
1660 1670 1680 1690 1700
LLTFHLNLTQ GTALIRYSTK QEAAKAQTAL HMCVLGNTTI LAEFATEDEV
1710 1720 1730 1740 1750
SRFLAQAQPP TPAATPSAPA TGWQSLETSQ NQADPVGPAL NLFGGSTGLG
1760 1770 1780 1790 1800
QWSSSAGGSS GADLAGTSLW GPPNYSSSLW GVPTVEDPHR MGSPAPLLPG
1810
DLLGGGSDSI
Length:1,810
Mass (Da):191,963
Last modified:May 5, 2009 - v2
Checksum:i70F0A3A88CE7C7C5
GO
Isoform 2 (identifier: Q8BKI2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-36: Missing.
     37-38: KQ → MR
     970-979: VWSKSTPPAP → GRYQLFSHRS
     980-1810: Missing.

Show »
Length:943
Mass (Da):99,891
Checksum:iB11D333CE3B24E15
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti904 – 9041D → Y in BAC34813 (PubMed:16141072).Curated
Sequence conflicti1576 – 15761T → P in BAC65722 (PubMed:12693553).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 3636Missing in isoform 2. 1 PublicationVSP_037294Add
BLAST
Alternative sequencei37 – 382KQ → MR in isoform 2. 1 PublicationVSP_037295
Alternative sequencei970 – 97910VWSKSTPPAP → GRYQLFSHRS in isoform 2. 1 PublicationVSP_037296
Alternative sequencei980 – 1810831Missing in isoform 2. 1 PublicationVSP_037297Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC125540 Genomic DNA. No translation available.
AC126682 Genomic DNA. No translation available.
AK051922 mRNA. Translation: BAC34813.1.
AK122440 mRNA. Translation: BAC65722.1.
CCDSiCCDS37146.1. [Q8BKI2-1]
RefSeqiNP_659061.2. NM_144812.2. [Q8BKI2-1]
UniGeneiMm.131328.
Mm.412092.

Genome annotation databases

EnsembliENSMUST00000067689; ENSMUSP00000064336; ENSMUSG00000047888. [Q8BKI2-1]
GeneIDi213988.
KEGGimmu:213988.
UCSCiuc007wvt.1. mouse. [Q8BKI2-1]
uc007wvu.2. mouse. [Q8BKI2-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC125540 Genomic DNA. No translation available.
AC126682 Genomic DNA. No translation available.
AK051922 mRNA. Translation: BAC34813.1.
AK122440 mRNA. Translation: BAC65722.1.
CCDSiCCDS37146.1. [Q8BKI2-1]
RefSeqiNP_659061.2. NM_144812.2. [Q8BKI2-1]
UniGeneiMm.131328.
Mm.412092.

3D structure databases

ProteinModelPortaliQ8BKI2.
SMRiQ8BKI2. Positions 1624-1704.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi229484. 1 interaction.
DIPiDIP-48568N.
IntActiQ8BKI2. 1 interaction.
STRINGi10090.ENSMUSP00000064336.

PTM databases

iPTMnetiQ8BKI2.
PhosphoSiteiQ8BKI2.

Proteomic databases

EPDiQ8BKI2.
MaxQBiQ8BKI2.
PaxDbiQ8BKI2.
PRIDEiQ8BKI2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000067689; ENSMUSP00000064336; ENSMUSG00000047888. [Q8BKI2-1]
GeneIDi213988.
KEGGimmu:213988.
UCSCiuc007wvt.1. mouse. [Q8BKI2-1]
uc007wvu.2. mouse. [Q8BKI2-2]

Organism-specific databases

CTDi23112.
MGIiMGI:2443730. Tnrc6b.
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410IET2. Eukaryota.
ENOG410YDKH. LUCA.
GeneTreeiENSGT00410000025966.
HOGENOMiHOG000049171.
InParanoidiQ8BKI2.
KOiK18412.
OMAiMPRPEGK.
OrthoDBiEOG7353VZ.
PhylomeDBiQ8BKI2.
TreeFamiTF329702.

Enzyme and pathway databases

ReactomeiR-MMU-426496. Post-transcriptional silencing by small RNAs.

Miscellaneous databases

ChiTaRSiTnrc6b. mouse.
PROiQ8BKI2.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BKI2.
GenevisibleiQ8BKI2. MM.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR019486. Argonaute_hook_dom.
IPR012677. Nucleotide-bd_a/b_plait.
IPR032226. TNRC6_PABC-bd.
[Graphical view]
PfamiPF10427. Ago_hook. 1 hit.
PF16608. TNRC6-PABC_bdg. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Eye.
  3. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
    DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1240-1810 (ISOFORM 1).
    Tissue: Brain.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1409; THR-1426; SER-1438 AND THR-1441, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiTNR6B_MOUSE
AccessioniPrimary (citable) accession number: Q8BKI2
Secondary accession number(s): Q80TK4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 5, 2009
Last modified: June 8, 2016
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.