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Protein

Target of rapamycin complex 2 subunit MAPKAP1

Gene

Mapkap1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Isoform 1 is involved in ciliogenesis, regulates cilia length through its interaction with CCDC28B independently of mTORC2 complex.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Stress response

Enzyme and pathway databases

ReactomeiR-MMU-1257604. PIP3 activates AKT signaling.
R-MMU-389357. CD28 dependent PI3K/Akt signaling.
R-MMU-5218920. VEGFR2 mediated vascular permeability.
R-MMU-6804757. Regulation of TP53 Degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Target of rapamycin complex 2 subunit MAPKAP1
Short name:
TORC2 subunit MAPKAP1
Alternative name(s):
Mitogen-activated protein kinase 2-associated protein 1
Stress-activated map kinase-interacting protein 1
Short name:
SAPK-interacting protein 1
Gene namesi
Name:Mapkap1
Synonyms:Mip1, Sin1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2444554. Mapkap1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane, Nucleus

Pathology & Biotechi

Disruption phenotypei

Death during early embryonic stages.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00003280332 – 522Target of rapamycin complex 2 subunit MAPKAP1Add BLAST521

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei186PhosphoserineBy similarity1
Modified residuei510PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ8BKH7.
PeptideAtlasiQ8BKH7.
PRIDEiQ8BKH7.

PTM databases

iPTMnetiQ8BKH7.
PhosphoSitePlusiQ8BKH7.

Expressioni

Tissue specificityi

Uniquitously expresseed, with highest levels in testis, kidney and liver. Present in renal tubule cells (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000038696.
ExpressionAtlasiQ8BKH7. baseline and differential.
GenevisibleiQ8BKH7. MM.

Interactioni

Subunit structurei

All isoforms except isoform 4 can be incorporated into the mammalian target of rapamycin complex 2 (mTORC2) which contains MTOR, MLST8, PRR5, RICTOR, MAPKAP1 and DEPTOR. Contrary to mTORC1, mTORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Interacts with ATF2, MAP3K2 and MAPK8. Interacts with GTP-bound HRAS and KRAS. Interacts with IFNAR2 and SGK1. Isoform 2 interacts with NBN. Isoform 1 interacts with CCDC28B.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi230681. 3 interactors.
DIPiDIP-57240N.
IntActiQ8BKH7. 7 interactors.
STRINGi10090.ENSMUSP00000108751.

Structurei

3D structure databases

ProteinModelPortaliQ8BKH7.
SMRiQ8BKH7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 267Interaction with NBNBy similarityAdd BLAST266
Regioni2 – 184Interaction with MAP3K2By similarityAdd BLAST183
Regioni468 – 522Interaction with ATF2By similarityAdd BLAST55

Sequence similaritiesi

Belongs to the SIN1 family.Curated

Phylogenomic databases

eggNOGiKOG3739. Eukaryota.
ENOG410XR57. LUCA.
GeneTreeiENSGT00390000000642.
HOVERGENiHBG023148.
InParanoidiQ8BKH7.
KOiK20410.
OMAiNAKFWPQ.
OrthoDBiEOG091G0AVW.
PhylomeDBiQ8BKH7.
TreeFamiTF315174.

Family and domain databases

InterProiIPR031567. CRIM_dom.
IPR008828. Sin1/Avo1.
IPR032679. Sin1_N.
IPR031313. Sin1_PH_dom.
[Graphical view]
PANTHERiPTHR13335. PTHR13335. 1 hit.
PfamiPF16978. CRIM. 1 hit.
PF05422. SIN1. 1 hit.
PF16979. SIN1_PH. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BKH7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAFLDNPTII LAHIRQSHVT SDDTGMCEMV LIDHDVDLEK THPPSVPGDS
60 70 80 90 100
GSEVQGSSGE TQGYIYAQSV DITSSWDFGI RRRSNTAQRL ERLRKERQNQ
110 120 130 140 150
IKCKNIQWKE RNSKQSAQEL KSLFEKKSLK EKPPSSGKQS ILSVRLEQCP
160 170 180 190 200
LQLNNPFNEY SKFDGKGHVG TTATKKIDVY LPLHSSQDRL LPMTVVTMAS
210 220 230 240 250
ARVQDLIGLI CWQYTSEGRE PKLNDNVSAY CLHIAEDDGE VDTDFPPLDS
260 270 280 290 300
NEPIHKFGFS TLALVEKYSS PGLTSKESLF VRINAAHGFS LIQVDNTKVT
310 320 330 340 350
MKEILLKAVK RRKGSQKISG PQYRLEKQSE PNIAVDLEST LESQNAWEFC
360 370 380 390 400
LVRENSSRAD GVFEEDSQID IATVQDMLSS HHYKSFKVSM IHRLRFTTDV
410 420 430 440 450
QLGISGDKVE IDPVTNQKAS TKFWIKQKPI SIDCDLLCAC DLAEEKSPSH
460 470 480 490 500
AVFKLTYLSS HDYKHLYFES DAATVSEIVL KVNYILESRA STARADYLAQ
510 520
KQRKLNRRTS FSFQKEKKSG QQ
Length:522
Mass (Da):59,009
Last modified:March 1, 2003 - v1
Checksum:i994C669D04065926
GO
Isoform 2 (identifier: Q8BKH7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     321-356: Missing.

Show »
Length:486
Mass (Da):54,802
Checksum:iF42F6C1899858727
GO
Isoform 3 (identifier: Q8BKH7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-192: Missing.

Show »
Length:330
Mass (Da):37,303
Checksum:iFA1ED1D41CF5CEA6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti516E → K in AAH43296 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0332081 – 192Missing in isoform 3. CuratedAdd BLAST192
Alternative sequenceiVSP_033209321 – 356Missing in isoform 2. CuratedAdd BLAST36

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK027932 mRNA. Translation: BAC25671.1.
AK052045 mRNA. Translation: BAC34838.1.
AK132263 mRNA. Translation: BAE21066.1.
AL845262, AL808102 Genomic DNA. Translation: CAM15450.1.
AL845262, AL808102 Genomic DNA. Translation: CAM15451.1.
AL845262, AL808102 Genomic DNA. Translation: CAM15454.1.
AL808102, AL845262 Genomic DNA. Translation: CAM17090.1.
AL808102, AL845262 Genomic DNA. Translation: CAM17091.1.
AL808102, AL845262 Genomic DNA. Translation: CAM17094.1.
BC027377 mRNA. Translation: AAH27377.1.
BC031579 mRNA. Translation: AAH31579.1.
BC043296 mRNA. Translation: AAH43296.1.
BC090644 mRNA. Translation: AAH90644.1.
BC096618 mRNA. Translation: AAH96618.1.
CCDSiCCDS15948.1. [Q8BKH7-1]
CCDS71035.1. [Q8BKH7-3]
RefSeqiNP_001277554.1. NM_001290625.1. [Q8BKH7-1]
NP_001277555.1. NM_001290626.1. [Q8BKH7-3]
NP_796319.1. NM_177345.4. [Q8BKH7-1]
XP_006498038.1. XM_006497975.2. [Q8BKH7-1]
XP_006498041.1. XM_006497978.3. [Q8BKH7-2]
XP_006498045.1. XM_006497982.2. [Q8BKH7-3]
XP_006498046.1. XM_006497983.2. [Q8BKH7-3]
XP_011237384.1. XM_011239082.2. [Q8BKH7-1]
XP_011237385.1. XM_011239083.2. [Q8BKH7-1]
XP_011237386.1. XM_011239084.2. [Q8BKH7-1]
XP_011237387.1. XM_011239085.2. [Q8BKH7-3]
XP_017172932.1. XM_017317443.1. [Q8BKH7-2]
UniGeneiMm.270866.
Mm.487284.

Genome annotation databases

EnsembliENSMUST00000113123; ENSMUSP00000108748; ENSMUSG00000038696. [Q8BKH7-3]
ENSMUST00000113124; ENSMUSP00000108749; ENSMUSG00000038696. [Q8BKH7-2]
ENSMUST00000113126; ENSMUSP00000108751; ENSMUSG00000038696. [Q8BKH7-1]
ENSMUST00000147337; ENSMUSP00000116494; ENSMUSG00000038696. [Q8BKH7-1]
GeneIDi227743.
KEGGimmu:227743.
UCSCiuc008jil.2. mouse. [Q8BKH7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK027932 mRNA. Translation: BAC25671.1.
AK052045 mRNA. Translation: BAC34838.1.
AK132263 mRNA. Translation: BAE21066.1.
AL845262, AL808102 Genomic DNA. Translation: CAM15450.1.
AL845262, AL808102 Genomic DNA. Translation: CAM15451.1.
AL845262, AL808102 Genomic DNA. Translation: CAM15454.1.
AL808102, AL845262 Genomic DNA. Translation: CAM17090.1.
AL808102, AL845262 Genomic DNA. Translation: CAM17091.1.
AL808102, AL845262 Genomic DNA. Translation: CAM17094.1.
BC027377 mRNA. Translation: AAH27377.1.
BC031579 mRNA. Translation: AAH31579.1.
BC043296 mRNA. Translation: AAH43296.1.
BC090644 mRNA. Translation: AAH90644.1.
BC096618 mRNA. Translation: AAH96618.1.
CCDSiCCDS15948.1. [Q8BKH7-1]
CCDS71035.1. [Q8BKH7-3]
RefSeqiNP_001277554.1. NM_001290625.1. [Q8BKH7-1]
NP_001277555.1. NM_001290626.1. [Q8BKH7-3]
NP_796319.1. NM_177345.4. [Q8BKH7-1]
XP_006498038.1. XM_006497975.2. [Q8BKH7-1]
XP_006498041.1. XM_006497978.3. [Q8BKH7-2]
XP_006498045.1. XM_006497982.2. [Q8BKH7-3]
XP_006498046.1. XM_006497983.2. [Q8BKH7-3]
XP_011237384.1. XM_011239082.2. [Q8BKH7-1]
XP_011237385.1. XM_011239083.2. [Q8BKH7-1]
XP_011237386.1. XM_011239084.2. [Q8BKH7-1]
XP_011237387.1. XM_011239085.2. [Q8BKH7-3]
XP_017172932.1. XM_017317443.1. [Q8BKH7-2]
UniGeneiMm.270866.
Mm.487284.

3D structure databases

ProteinModelPortaliQ8BKH7.
SMRiQ8BKH7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230681. 3 interactors.
DIPiDIP-57240N.
IntActiQ8BKH7. 7 interactors.
STRINGi10090.ENSMUSP00000108751.

PTM databases

iPTMnetiQ8BKH7.
PhosphoSitePlusiQ8BKH7.

Proteomic databases

PaxDbiQ8BKH7.
PeptideAtlasiQ8BKH7.
PRIDEiQ8BKH7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000113123; ENSMUSP00000108748; ENSMUSG00000038696. [Q8BKH7-3]
ENSMUST00000113124; ENSMUSP00000108749; ENSMUSG00000038696. [Q8BKH7-2]
ENSMUST00000113126; ENSMUSP00000108751; ENSMUSG00000038696. [Q8BKH7-1]
ENSMUST00000147337; ENSMUSP00000116494; ENSMUSG00000038696. [Q8BKH7-1]
GeneIDi227743.
KEGGimmu:227743.
UCSCiuc008jil.2. mouse. [Q8BKH7-1]

Organism-specific databases

CTDi79109.
MGIiMGI:2444554. Mapkap1.

Phylogenomic databases

eggNOGiKOG3739. Eukaryota.
ENOG410XR57. LUCA.
GeneTreeiENSGT00390000000642.
HOVERGENiHBG023148.
InParanoidiQ8BKH7.
KOiK20410.
OMAiNAKFWPQ.
OrthoDBiEOG091G0AVW.
PhylomeDBiQ8BKH7.
TreeFamiTF315174.

Enzyme and pathway databases

ReactomeiR-MMU-1257604. PIP3 activates AKT signaling.
R-MMU-389357. CD28 dependent PI3K/Akt signaling.
R-MMU-5218920. VEGFR2 mediated vascular permeability.
R-MMU-6804757. Regulation of TP53 Degradation.

Miscellaneous databases

ChiTaRSiMapkap1. mouse.
PROiQ8BKH7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038696.
ExpressionAtlasiQ8BKH7. baseline and differential.
GenevisibleiQ8BKH7. MM.

Family and domain databases

InterProiIPR031567. CRIM_dom.
IPR008828. Sin1/Avo1.
IPR032679. Sin1_N.
IPR031313. Sin1_PH_dom.
[Graphical view]
PANTHERiPTHR13335. PTHR13335. 1 hit.
PfamiPF16978. CRIM. 1 hit.
PF05422. SIN1. 1 hit.
PF16979. SIN1_PH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSIN1_MOUSE
AccessioniPrimary (citable) accession number: Q8BKH7
Secondary accession number(s): A2AN72
, A2AN74, A2AR13, A2AR16, Q80UY4, Q8BMV5, Q8R2N9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.