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Q8BKH7

- SIN1_MOUSE

UniProt

Q8BKH7 - SIN1_MOUSE

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Protein
Target of rapamycin complex 2 subunit MAPKAP1
Gene
Mapkap1, Mip1, Sin1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Isoform 1 is involved in ciliogenesis, regulates cilia length through its interaction with CCDC28B independently of mTORC2 complex.1 Publication

GO - Molecular functioni

  1. phosphatidic acid binding Source: Ensembl
  2. phosphatidylinositol-3,4,5-trisphosphate binding Source: Ensembl
  3. phosphatidylinositol-3,4-bisphosphate binding Source: Ensembl
  4. phosphatidylinositol-3,5-bisphosphate binding Source: Ensembl
  5. phosphatidylinositol-4,5-bisphosphate binding Source: Ensembl
  6. protein binding Source: UniProtKB

GO - Biological processi

  1. negative regulation of Ras protein signal transduction Source: Ensembl
  2. response to stress Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Stress response

Enzyme and pathway databases

ReactomeiREACT_196588. Constitutive PI3K/AKT Signaling in Cancer.
REACT_226151. CD28 dependent PI3K/Akt signaling.
REACT_226341. PIP3 activates AKT signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Target of rapamycin complex 2 subunit MAPKAP1
Short name:
TORC2 subunit MAPKAP1
Alternative name(s):
Mitogen-activated protein kinase 2-associated protein 1
Stress-activated map kinase-interacting protein 1
Short name:
SAPK-interacting protein 1
Gene namesi
Name:Mapkap1
Synonyms:Mip1, Sin1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:2444554. Mapkap1.

Subcellular locationi

Cell membrane; Peripheral membrane protein By similarity. Cytoplasmic vesicle By similarity. Nucleus By similarity

GO - Cellular componenti

  1. Golgi apparatus Source: Ensembl
  2. cytoplasmic membrane-bounded vesicle Source: UniProtKB-SubCell
  3. nucleus Source: UniProtKB-SubCell
  4. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane, Nucleus

Pathology & Biotechi

Disruption phenotypei

Death during early embryonic stages.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 522521Target of rapamycin complex 2 subunit MAPKAP1
PRO_0000328033Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine By similarity
Modified residuei510 – 5101Phosphoserine By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiQ8BKH7.

PTM databases

PhosphoSiteiQ8BKH7.

Expressioni

Tissue specificityi

Uniquitously expresseed, with highest levels in testis, kidney and liver. Present in renal tubule cells (at protein level).1 Publication

Gene expression databases

ArrayExpressiQ8BKH7.
BgeeiQ8BKH7.
GenevestigatoriQ8BKH7.

Interactioni

Subunit structurei

All isoforms except isoform 4 can be incorporated into the mammalian target of rapamycin complex 2 (mTORC2) which contains MTOR, MLST8, PRR5, RICTOR, MAPKAP1 and DEPTOR. Contrary to mTORC1, mTORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Interacts with ATF2, MAP3K2 and MAPK8. Interacts with GTP-bound HRAS and KRAS. Interacts with IFNAR2 and SGK1. Isoform 2 interacts with NBN. Isoform 1 interacts with CCDC28B.1 Publication

Protein-protein interaction databases

DIPiDIP-57240N.
IntActiQ8BKH7. 7 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ8BKH7.
SMRiQ8BKH7. Positions 371-490.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 267266Interaction with NBN By similarity
Add
BLAST
Regioni2 – 184183Interaction with MAP3K2 By similarity
Add
BLAST
Regioni468 – 52255Interaction with ATF2 By similarity
Add
BLAST

Sequence similaritiesi

Belongs to the SIN1 family.

Phylogenomic databases

eggNOGiNOG303753.
GeneTreeiENSGT00390000000642.
HOVERGENiHBG023148.
InParanoidiQ8BKH7.
OMAiLCACDLV.
PhylomeDBiQ8BKH7.
TreeFamiTF315174.

Family and domain databases

InterProiIPR008828. SIN1.
[Graphical view]
PANTHERiPTHR13335. PTHR13335. 1 hit.
PfamiPF05422. SIN1. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8BKH7-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAFLDNPTII LAHIRQSHVT SDDTGMCEMV LIDHDVDLEK THPPSVPGDS    50
GSEVQGSSGE TQGYIYAQSV DITSSWDFGI RRRSNTAQRL ERLRKERQNQ 100
IKCKNIQWKE RNSKQSAQEL KSLFEKKSLK EKPPSSGKQS ILSVRLEQCP 150
LQLNNPFNEY SKFDGKGHVG TTATKKIDVY LPLHSSQDRL LPMTVVTMAS 200
ARVQDLIGLI CWQYTSEGRE PKLNDNVSAY CLHIAEDDGE VDTDFPPLDS 250
NEPIHKFGFS TLALVEKYSS PGLTSKESLF VRINAAHGFS LIQVDNTKVT 300
MKEILLKAVK RRKGSQKISG PQYRLEKQSE PNIAVDLEST LESQNAWEFC 350
LVRENSSRAD GVFEEDSQID IATVQDMLSS HHYKSFKVSM IHRLRFTTDV 400
QLGISGDKVE IDPVTNQKAS TKFWIKQKPI SIDCDLLCAC DLAEEKSPSH 450
AVFKLTYLSS HDYKHLYFES DAATVSEIVL KVNYILESRA STARADYLAQ 500
KQRKLNRRTS FSFQKEKKSG QQ 522
Length:522
Mass (Da):59,009
Last modified:March 1, 2003 - v1
Checksum:i994C669D04065926
GO
Isoform 2 (identifier: Q8BKH7-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     321-356: Missing.

Show »
Length:486
Mass (Da):54,802
Checksum:iF42F6C1899858727
GO
Isoform 3 (identifier: Q8BKH7-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     2-192: Missing.

Show »
Length:331
Mass (Da):37,434
Checksum:iCB7495E7C17E147F
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei2 – 192191Missing in isoform 3.
VSP_033208Add
BLAST
Alternative sequencei321 – 35636Missing in isoform 2.
VSP_033209Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti516 – 5161E → K in AAH43296. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK027932 mRNA. Translation: BAC25671.1.
AK052045 mRNA. Translation: BAC34838.1.
AK132263 mRNA. Translation: BAE21066.1.
AL845262, AL808102 Genomic DNA. Translation: CAM15450.1.
AL845262, AL808102 Genomic DNA. Translation: CAM15451.1.
AL845262, AL808102 Genomic DNA. Translation: CAM15454.1.
AL808102, AL845262 Genomic DNA. Translation: CAM17090.1.
AL808102, AL845262 Genomic DNA. Translation: CAM17091.1.
AL808102, AL845262 Genomic DNA. Translation: CAM17094.1.
BC027377 mRNA. Translation: AAH27377.1.
BC031579 mRNA. Translation: AAH31579.1.
BC043296 mRNA. Translation: AAH43296.1.
BC090644 mRNA. Translation: AAH90644.1.
BC096618 mRNA. Translation: AAH96618.1.
CCDSiCCDS15948.1. [Q8BKH7-1]
RefSeqiNP_001277554.1. NM_001290625.1. [Q8BKH7-1]
NP_001277555.1. NM_001290626.1.
NP_796319.1. NM_177345.4. [Q8BKH7-1]
XP_006498036.1. XM_006497973.1. [Q8BKH7-1]
XP_006498038.1. XM_006497975.1. [Q8BKH7-1]
XP_006498039.1. XM_006497976.1. [Q8BKH7-1]
XP_006498041.1. XM_006497978.1. [Q8BKH7-2]
UniGeneiMm.270866.
Mm.487284.

Genome annotation databases

EnsembliENSMUST00000113124; ENSMUSP00000108749; ENSMUSG00000038696. [Q8BKH7-2]
ENSMUST00000113126; ENSMUSP00000108751; ENSMUSG00000038696. [Q8BKH7-1]
ENSMUST00000147337; ENSMUSP00000116494; ENSMUSG00000038696. [Q8BKH7-1]
GeneIDi227743.
KEGGimmu:227743.
UCSCiuc008jil.1. mouse. [Q8BKH7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK027932 mRNA. Translation: BAC25671.1 .
AK052045 mRNA. Translation: BAC34838.1 .
AK132263 mRNA. Translation: BAE21066.1 .
AL845262 , AL808102 Genomic DNA. Translation: CAM15450.1 .
AL845262 , AL808102 Genomic DNA. Translation: CAM15451.1 .
AL845262 , AL808102 Genomic DNA. Translation: CAM15454.1 .
AL808102 , AL845262 Genomic DNA. Translation: CAM17090.1 .
AL808102 , AL845262 Genomic DNA. Translation: CAM17091.1 .
AL808102 , AL845262 Genomic DNA. Translation: CAM17094.1 .
BC027377 mRNA. Translation: AAH27377.1 .
BC031579 mRNA. Translation: AAH31579.1 .
BC043296 mRNA. Translation: AAH43296.1 .
BC090644 mRNA. Translation: AAH90644.1 .
BC096618 mRNA. Translation: AAH96618.1 .
CCDSi CCDS15948.1. [Q8BKH7-1 ]
RefSeqi NP_001277554.1. NM_001290625.1. [Q8BKH7-1 ]
NP_001277555.1. NM_001290626.1.
NP_796319.1. NM_177345.4. [Q8BKH7-1 ]
XP_006498036.1. XM_006497973.1. [Q8BKH7-1 ]
XP_006498038.1. XM_006497975.1. [Q8BKH7-1 ]
XP_006498039.1. XM_006497976.1. [Q8BKH7-1 ]
XP_006498041.1. XM_006497978.1. [Q8BKH7-2 ]
UniGenei Mm.270866.
Mm.487284.

3D structure databases

ProteinModelPortali Q8BKH7.
SMRi Q8BKH7. Positions 371-490.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-57240N.
IntActi Q8BKH7. 7 interactions.

PTM databases

PhosphoSitei Q8BKH7.

Proteomic databases

PRIDEi Q8BKH7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000113124 ; ENSMUSP00000108749 ; ENSMUSG00000038696 . [Q8BKH7-2 ]
ENSMUST00000113126 ; ENSMUSP00000108751 ; ENSMUSG00000038696 . [Q8BKH7-1 ]
ENSMUST00000147337 ; ENSMUSP00000116494 ; ENSMUSG00000038696 . [Q8BKH7-1 ]
GeneIDi 227743.
KEGGi mmu:227743.
UCSCi uc008jil.1. mouse. [Q8BKH7-1 ]

Organism-specific databases

CTDi 79109.
MGIi MGI:2444554. Mapkap1.

Phylogenomic databases

eggNOGi NOG303753.
GeneTreei ENSGT00390000000642.
HOVERGENi HBG023148.
InParanoidi Q8BKH7.
OMAi LCACDLV.
PhylomeDBi Q8BKH7.
TreeFami TF315174.

Enzyme and pathway databases

Reactomei REACT_196588. Constitutive PI3K/AKT Signaling in Cancer.
REACT_226151. CD28 dependent PI3K/Akt signaling.
REACT_226341. PIP3 activates AKT signaling.

Miscellaneous databases

ChiTaRSi MAPKAP1. mouse.
NextBioi 378816.
PROi Q8BKH7.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q8BKH7.
Bgeei Q8BKH7.
Genevestigatori Q8BKH7.

Family and domain databases

InterProi IPR008828. SIN1.
[Graphical view ]
PANTHERi PTHR13335. PTHR13335. 1 hit.
Pfami PF05422. SIN1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Embryo, Eye and Placenta.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6 and FVB/N.
    Tissue: Brain, Mammary tumor and Olfactory epithelium.
  4. "SIN1/MIP1 maintains rictor-mTOR complex integrity and regulates Akt phosphorylation and substrate specificity."
    Jacinto E., Facchinetti V., Liu D., Soto N., Wei S., Jung S.Y., Huang Q., Qin J., Su B.
    Cell 127:125-137(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  5. "Sin1 binds to both ATF-2 and p38 and enhances ATF-2-dependent transcription in an SAPK signaling pathway."
    Makino C., Sano Y., Shinagawa T., Millar J.B., Ishii S.
    Genes Cells 11:1239-1251(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. "mSIN1 protein mediates SGK1 protein interaction with mTORC2 protein complex and is required for selective activation of the epithelial sodium channel."
    Lu M., Wang J., Ives H.E., Pearce D.
    J. Biol. Chem. 286:30647-30654(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SGK1.

Entry informationi

Entry nameiSIN1_MOUSE
AccessioniPrimary (citable) accession number: Q8BKH7
Secondary accession number(s): A2AN72
, A2AN74, A2AR13, A2AR16, Q80UY4, Q8BMV5, Q8R2N9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: March 1, 2003
Last modified: September 3, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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