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Q8BKF1 (RPOM_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-directed RNA polymerase, mitochondrial

Short name=MtRPOL
EC=2.7.7.6
Gene names
Name:Polrmt
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1207 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates.

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Subunit structure

Interacts with TFB1M and TFB2M, leading to the stimulation of transcription. Interacts with TEFM By similarity.

Subcellular location

Mitochondrion By similarity.

Sequence similarities

Belongs to the phage and mitochondrial RNA polymerase family.

Contains 2 PPR (pentatricopeptide) repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4141Mitochondrion Potential
Chain42 – 12071166DNA-directed RNA polymerase, mitochondrial
PRO_0000031069

Regions

Repeat232 – 26635PPR 1
Repeat267 – 30236PPR 2
Region773 – 1207435Mediates interaction with TEFM By similarity

Sites

Active site8931 By similarity
Active site9621 By similarity
Active site11211 By similarity

Experimental info

Sequence conflict231H → Q in BAC39500. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q8BKF1 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 674601A6BC001CE4

FASTA1,207136,705
        10         20         30         40         50         60 
MSALRWTRSA AGLGRVLRSP GPHRPPSEEG TFGGFCSSRR SSAASPREQH VLREWGHAEL 

        70         80         90        100        110        120 
LEVLEARVRQ LRAEGTPEMR VKKVQVDRPP QGHSSRWAQK LEAEKRVKQR RQKEVDQQKQ 

       130        140        150        160        170        180 
ALTQEFWTLH KEPKIWNKKL AGYLQPSKKG TPTNSEEKQL AQALQAALGR LSSREAEALA 

       190        200        210        220        230        240 
RKKAKAVEAQ ILVLQQKFLA FFECCVCTGQ VPLAHHVLVT HHNNGDRQQV LTLHMYNTVM 

       250        260        270        280        290        300 
LGWARKGSFR ELVYVFLMLK DAGLSPDLCS YAAALQCMGR RDQDVRTIQR CLKQMMEEGF 

       310        320        330        340        350        360 
QPQLLFTDLV LEEEDRAALL RAVVKAEPAF RPPPQAPSPV NTSTLLKDIY SKEGPVSYPK 

       370        380        390        400        410        420 
LHLPLDTLQD LFYQQLHVEL SSSVCVQSVE KAPVMSKEVI EARKTLQALR EQWEVELLRV 

       430        440        450        460        470        480 
LRETKATMGR QAYEGQPTLY PFLCLLSEGE FVSILMQVLK VLPAQGEPLI QLAHNLGLRV 

       490        500        510        520        530        540 
LNRHLVKQKQ VTNHVQKLGQ RYSQYLQLLA SDTQVAPCLP REYWESLGPL EAPAQQPWSV 

       550        560        570        580        590        600 
PVLLQLGKQL AELLVQAVQM PRSLAARQGA QRSIPVLYHV YSFRSYRQVG ILKPHPAFTH 

       610        620        630        640        650        660 
LLETAAEPTL TFETTEVPML CPPLPWTSLH SGAYLLSSTK LMRATEGTTQ HQRLLEQCPP 

       670        680        690        700        710        720 
AQLHGPLDAL TQLGNCAWRV NGHLLDLVLQ IFRDKGCMPL GVPPPRSEAP RPARYQLPPG 

       730        740        750        760        770        780 
STPVHKSELR KELARCLKVA REMHSLRSEA LYRLSLAQHL RHRVFWLPHN MDFRGRTYPC 

       790        800        810        820        830        840 
PPHFNHLGSD LARALLEFAE GRPLGPRGLD WLKIHLINLT GLKKGDSLRM RLAFADEVME 

       850        860        870        880        890        900 
EILDSADNPL TGRKWWMEAD EPWQTLACCM EVAHAVRSPD PAAYISHLPV HQDGSCNGLQ 

       910        920        930        940        950        960 
HYAALGRDSV GAASVNLTPS DLPQDVYREV ATQVEEFRQQ DAKEGLRVAQ VLEGFISRKV 

       970        980        990       1000       1010       1020 
VKQTVMTVVY GVTRYGGRLQ IEKRLRELSD FPQEFVWEAS HYLVRQVFKS LQEMFTSTRA 

      1030       1040       1050       1060       1070       1080 
IQHWLTESAN LISHAGWPVE WVTPLGIPII QPYHRESKVQ VKGGLQSITL TSSVDESQKP 

      1090       1100       1110       1120       1130       1140 
NTLKQKNGFP PNFIHSLDSS HMMLTALHCY RKGLIFVSVH DCFWTHAADI PTMNEVCREQ 

      1150       1160       1170       1180       1190       1200 
FVRLHSQPIL EDLAKFLKKR FCSVSSIKSL KSSERALVTK LQETLQSLPK TGTFDLGQVI 


RSTYFFS 

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References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Eye and Mammary gland.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK053356 mRNA. Translation: BAC35360.1.
AK085666 mRNA. Translation: BAC39500.1.
CCDSCCDS23987.1.
RefSeqNP_766139.2. NM_172551.3.
UniGeneMm.39001.

3D structure databases

ProteinModelPortalQ8BKF1.
SMRQ8BKF1. Positions 195-1207.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000020580.

PTM databases

PhosphoSiteQ8BKF1.

Proteomic databases

PaxDbQ8BKF1.
PRIDEQ8BKF1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000020580; ENSMUSP00000020580; ENSMUSG00000020329.
GeneID216151.
KEGGmmu:216151.
UCSCuc007fzo.2. mouse.

Organism-specific databases

CTD5442.
MGIMGI:1915843. Polrmt.

Phylogenomic databases

eggNOGCOG5108.
GeneTreeENSGT00390000008060.
HOGENOMHOG000044061.
HOVERGENHBG078730.
InParanoidQ8BKF1.
KOK10908.
OMAREMFTST.
OrthoDBEOG7RBZ7T.
PhylomeDBQ8BKF1.
TreeFamTF105700.

Gene expression databases

ArrayExpressQ8BKF1.
BgeeQ8BKF1.
CleanExMM_POLRMT.
GenevestigatorQ8BKF1.

Family and domain databases

Gene3D1.10.1320.10. 1 hit.
1.10.287.260. 1 hit.
InterProIPR024075. DNA-dir_RNA_pol_helix_hairpin.
IPR002092. DNA-dir_Rpol_phage-type.
IPR002885. Pentatricopeptide_repeat.
IPR029262. RPOL_N.
[Graphical view]
PANTHERPTHR10102. PTHR10102. 1 hit.
PfamPF00940. RNA_pol. 1 hit.
PF14700. RPOL_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR00756. PPR. 1 hit.
PROSITEPS51375. PPR. 2 hits.
PS00900. RNA_POL_PHAGE_1. 1 hit.
PS00489. RNA_POL_PHAGE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio375012.
PROQ8BKF1.
SOURCESearch...

Entry information

Entry nameRPOM_MOUSE
AccessionPrimary (citable) accession number: Q8BKF1
Secondary accession number(s): Q8BJE0
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot