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Protein

DNA-directed RNA polymerase, mitochondrial

Gene

Polrmt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei893By similarity1
Active sitei962By similarity1
Active sitei1121By similarity1

GO - Molecular functioni

  • DNA-directed 5'-3' RNA polymerase activity Source: MGI
  • sequence-specific DNA binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processTranscription

Enzyme and pathway databases

ReactomeiR-MMU-163282 Mitochondrial transcription initiation

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase, mitochondrial (EC:2.7.7.6)
Short name:
MtRPOL
Gene namesi
Name:Polrmt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1915843 Polrmt

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

DNA-directed RNA polymerase, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 41MitochondrionSequence analysisAdd BLAST41
ChainiPRO_000003106942 – 1207DNA-directed RNA polymerase, mitochondrialAdd BLAST1166

Proteomic databases

EPDiQ8BKF1
MaxQBiQ8BKF1
PaxDbiQ8BKF1
PRIDEiQ8BKF1

PTM databases

iPTMnetiQ8BKF1
PhosphoSitePlusiQ8BKF1

Expressioni

Gene expression databases

BgeeiENSMUSG00000020329
CleanExiMM_POLRMT
ExpressionAtlasiQ8BKF1 baseline and differential
GenevisibleiQ8BKF1 MM

Interactioni

Subunit structurei

Interacts with TFB1M and TFB2M, leading to the stimulation of transcription. Interacts with TEFM (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020580

Structurei

3D structure databases

ProteinModelPortaliQ8BKF1
SMRiQ8BKF1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati232 – 266PPR 1Add BLAST35
Repeati267 – 302PPR 2Add BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni773 – 1207Mediates interaction with TEFMBy similarityAdd BLAST435

Sequence similaritiesi

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiKOG1038 Eukaryota
COG5108 LUCA
GeneTreeiENSGT00390000008060
HOGENOMiHOG000044061
HOVERGENiHBG078730
InParanoidiQ8BKF1
KOiK10908
OMAiDWFTECA
OrthoDBiEOG091G00YJ
PhylomeDBiQ8BKF1
TreeFamiTF105700

Family and domain databases

Gene3Di1.10.1320.10, 1 hit
1.25.40.10, 1 hit
InterProiView protein in InterPro
IPR002092 DNA-dir_Rpol_phage-type
IPR002885 Pentatricopeptide_repeat
IPR037159 RNA_POL_N_sf
IPR029262 RPOL_N
IPR011990 TPR-like_helical_dom_sf
PANTHERiPTHR10102 PTHR10102, 1 hit
PfamiView protein in Pfam
PF00940 RNA_pol, 1 hit
PF14700 RPOL_N, 1 hit
SMARTiView protein in SMART
SM01311 RPOL_N, 1 hit
TIGRFAMsiTIGR00756 PPR, 1 hit
PROSITEiView protein in PROSITE
PS51375 PPR, 2 hits
PS00900 RNA_POL_PHAGE_1, 1 hit
PS00489 RNA_POL_PHAGE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8BKF1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSALRWTRSA AGLGRVLRSP GPHRPPSEEG TFGGFCSSRR SSAASPREQH
60 70 80 90 100
VLREWGHAEL LEVLEARVRQ LRAEGTPEMR VKKVQVDRPP QGHSSRWAQK
110 120 130 140 150
LEAEKRVKQR RQKEVDQQKQ ALTQEFWTLH KEPKIWNKKL AGYLQPSKKG
160 170 180 190 200
TPTNSEEKQL AQALQAALGR LSSREAEALA RKKAKAVEAQ ILVLQQKFLA
210 220 230 240 250
FFECCVCTGQ VPLAHHVLVT HHNNGDRQQV LTLHMYNTVM LGWARKGSFR
260 270 280 290 300
ELVYVFLMLK DAGLSPDLCS YAAALQCMGR RDQDVRTIQR CLKQMMEEGF
310 320 330 340 350
QPQLLFTDLV LEEEDRAALL RAVVKAEPAF RPPPQAPSPV NTSTLLKDIY
360 370 380 390 400
SKEGPVSYPK LHLPLDTLQD LFYQQLHVEL SSSVCVQSVE KAPVMSKEVI
410 420 430 440 450
EARKTLQALR EQWEVELLRV LRETKATMGR QAYEGQPTLY PFLCLLSEGE
460 470 480 490 500
FVSILMQVLK VLPAQGEPLI QLAHNLGLRV LNRHLVKQKQ VTNHVQKLGQ
510 520 530 540 550
RYSQYLQLLA SDTQVAPCLP REYWESLGPL EAPAQQPWSV PVLLQLGKQL
560 570 580 590 600
AELLVQAVQM PRSLAARQGA QRSIPVLYHV YSFRSYRQVG ILKPHPAFTH
610 620 630 640 650
LLETAAEPTL TFETTEVPML CPPLPWTSLH SGAYLLSSTK LMRATEGTTQ
660 670 680 690 700
HQRLLEQCPP AQLHGPLDAL TQLGNCAWRV NGHLLDLVLQ IFRDKGCMPL
710 720 730 740 750
GVPPPRSEAP RPARYQLPPG STPVHKSELR KELARCLKVA REMHSLRSEA
760 770 780 790 800
LYRLSLAQHL RHRVFWLPHN MDFRGRTYPC PPHFNHLGSD LARALLEFAE
810 820 830 840 850
GRPLGPRGLD WLKIHLINLT GLKKGDSLRM RLAFADEVME EILDSADNPL
860 870 880 890 900
TGRKWWMEAD EPWQTLACCM EVAHAVRSPD PAAYISHLPV HQDGSCNGLQ
910 920 930 940 950
HYAALGRDSV GAASVNLTPS DLPQDVYREV ATQVEEFRQQ DAKEGLRVAQ
960 970 980 990 1000
VLEGFISRKV VKQTVMTVVY GVTRYGGRLQ IEKRLRELSD FPQEFVWEAS
1010 1020 1030 1040 1050
HYLVRQVFKS LQEMFTSTRA IQHWLTESAN LISHAGWPVE WVTPLGIPII
1060 1070 1080 1090 1100
QPYHRESKVQ VKGGLQSITL TSSVDESQKP NTLKQKNGFP PNFIHSLDSS
1110 1120 1130 1140 1150
HMMLTALHCY RKGLIFVSVH DCFWTHAADI PTMNEVCREQ FVRLHSQPIL
1160 1170 1180 1190 1200
EDLAKFLKKR FCSVSSIKSL KSSERALVTK LQETLQSLPK TGTFDLGQVI

RSTYFFS
Length:1,207
Mass (Da):136,705
Last modified:March 1, 2003 - v1
Checksum:i674601A6BC001CE4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti23H → Q in BAC39500 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK053356 mRNA Translation: BAC35360.1
AK085666 mRNA Translation: BAC39500.1
CCDSiCCDS23987.1
RefSeqiNP_766139.2, NM_172551.3
UniGeneiMm.39001

Genome annotation databases

EnsembliENSMUST00000020580; ENSMUSP00000020580; ENSMUSG00000020329
GeneIDi216151
KEGGimmu:216151
UCSCiuc007fzo.2 mouse

Similar proteinsi

Entry informationi

Entry nameiRPOM_MOUSE
AccessioniPrimary (citable) accession number: Q8BKF1
Secondary accession number(s): Q8BJE0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: March 1, 2003
Last modified: April 25, 2018
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health