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Protein

Intraflagellar transport protein 74 homolog

Gene

Ift74

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the intraflagellar transport (IFT) complex B: together with IFT81, forms a tubulin-binding module that specifically mediates transport of tubulin within the cilium. Binds beta-tubulin via its basic region. Required for ciliogenesis (By similarity).By similarity

GO - Molecular functioni

  • beta-tubulin binding Source: UniProtKB
  • chromatin binding Source: MGI

GO - Biological processi

  • cilium assembly Source: UniProtKB
  • cilium morphogenesis Source: MGI
  • determination of left/right symmetry Source: MGI
  • epidermis development Source: MGI
  • heart development Source: MGI
  • intraciliary transport Source: GO_Central
  • intraciliary transport involved in cilium morphogenesis Source: UniProtKB
  • keratinocyte development Source: MGI
  • negative regulation of epithelial cell proliferation Source: MGI
  • Notch signaling pathway Source: MGI
  • positive regulation of cell adhesion mediated by integrin Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cilium biogenesis/degradation

Enzyme and pathway databases

ReactomeiR-MMU-5620924. Intraflagellar transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Intraflagellar transport protein 74 homolog
Alternative name(s):
Capillary morphogenesis gene 1 protein
Short name:
CMG-1
Coiled-coil domain-containing protein 2
Gene namesi
Name:Ift74
Synonyms:Ccdc2, Cmg1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1914944. Ift74.

Subcellular locationi

GO - Cellular componenti

  • centrosome Source: MGI
  • cilium Source: MGI
  • cytoplasmic, membrane-bounded vesicle Source: UniProtKB-SubCell
  • intraciliary transport particle B Source: UniProtKB
  • nucleus Source: MGI
  • primary cilium Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasmic vesicle

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 600600Intraflagellar transport protein 74 homologPRO_0000084170Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei73 – 731PhosphothreonineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8BKE9.
PaxDbiQ8BKE9.
PRIDEiQ8BKE9.

PTM databases

iPTMnetiQ8BKE9.
PhosphoSiteiQ8BKE9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000028576.
ExpressionAtlasiQ8BKE9. baseline and differential.
GenevisibleiQ8BKE9. MM.

Interactioni

Subunit structurei

Component of the IFT complex B, at least composed of IFT20, IFT22, HSPB11/IFT25, IFT27, IFT46, IFT52, TRAF3IP1/IFT54, IFT57, IFT74, IFT80, IFT81, and IFT88. Interacts with IFT81; the interaction is direct: within the IFT complex B, IFT74 and IFT81 mediate the transport of tubulin within the cilium. Interacts (via basic region) with beta-tubulin (via acidic region); interaction is direct. Interacts with ARL13B and IFT88.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi212373. 1 interaction.
STRINGi10090.ENSMUSP00000030311.

Structurei

3D structure databases

ProteinModelPortaliQ8BKE9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 9090Basic regionBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili97 – 390294Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi497 – 5004Poly-Lys

Sequence similaritiesi

Belongs to the IFT74 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IFCX. Eukaryota.
ENOG410XWAZ. LUCA.
GeneTreeiENSGT00390000007109.
HOGENOMiHOG000031494.
HOVERGENiHBG050820.
InParanoidiQ8BKE9.
KOiK19679.
OMAiREENMDA.
OrthoDBiEOG091G05UB.
PhylomeDBiQ8BKE9.
TreeFamiTF318352.

Family and domain databases

InterProiIPR029602. IFT74.
[Graphical view]
PANTHERiPTHR31432:SF0. PTHR31432:SF0. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8BKE9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASNHKSSAP RPISRGGIGL TGRPPSGIRP PSGNVRVATA MPPTTARPGS
60 70 80 90 100
RGGPLGTGGV LSSQIKVADR PVTQQGLSGM KTGMKGPQRQ ILDKSYYLGL
110 120 130 140 150
LRSKISELTT EINKLQKEIE MYNQENSVYL SYEKRAETLA VEIKDFQGQL
160 170 180 190 200
ADYNMLVDKL NTNTEMEEVM SDYNMLKAQN DRETQSMDVI FTERQAKEKQ
210 220 230 240 250
IRSVEEEVEQ EKQAADGIIK NMSPEKQVKY IEMKTTNEKL LQELDTLQQQ
260 270 280 290 300
LDSLNMKKES LETEIAHSQV KQEAVLLYEK LYELESHRDQ MIAEDKSMGS
310 320 330 340 350
PMEERERLLK QVKEDNQEIA SMERQLTDIK EKINQFSEEI RQLDMDLEEH
360 370 380 390 400
QGEMNQKYKE LKKREENMDA FIETFEETKN QELERKAQIE ASIITLLEHC
410 420 430 440 450
SRNINRMKQI SSITNQELKM MQDDLSFKST EMQKSQTTAR NLTSDSQRLQ
460 470 480 490 500
LDLQKMELLE SKMTEEQQSL KNKIKQMTAD LETYSDLAAL KSSAEEKKKK
510 520 530 540 550
LHQERTVLST HRNAFKKIME KLTSDYDTLK RQLQDNETHA QLTNLERKWQ
560 570 580 590 600
HLEQNNFVMK EFIATKSQES DYQPVIKNVM KQIAEYNKTI MDALHNASRS
Length:600
Mass (Da):69,301
Last modified:July 19, 2004 - v2
Checksum:i671606E5CA9096D5
GO

Sequence cautioni

The sequence BAB24617 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK006492 mRNA. Translation: BAB24617.1. Different initiation.
AK011690 mRNA. Translation: BAB27780.1.
AK014770 mRNA. Translation: BAB29542.3.
AK053369 mRNA. Translation: BAC35365.1.
AL732597, AL772277 Genomic DNA. Translation: CAM19637.1.
AL772277, AL732597 Genomic DNA. Translation: CAM23753.1.
CCDSiCCDS51232.1.
RefSeqiNP_001277497.1. NM_001290568.1.
NP_080595.2. NM_026319.3.
XP_006503387.1. XM_006503324.2.
XP_006503389.1. XM_006503326.1.
XP_011238899.1. XM_011240597.1.
UniGeneiMm.240619.

Genome annotation databases

EnsembliENSMUST00000030311; ENSMUSP00000030311; ENSMUSG00000028576.
GeneIDi67694.
KEGGimmu:67694.
UCSCiuc008tsg.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK006492 mRNA. Translation: BAB24617.1. Different initiation.
AK011690 mRNA. Translation: BAB27780.1.
AK014770 mRNA. Translation: BAB29542.3.
AK053369 mRNA. Translation: BAC35365.1.
AL732597, AL772277 Genomic DNA. Translation: CAM19637.1.
AL772277, AL732597 Genomic DNA. Translation: CAM23753.1.
CCDSiCCDS51232.1.
RefSeqiNP_001277497.1. NM_001290568.1.
NP_080595.2. NM_026319.3.
XP_006503387.1. XM_006503324.2.
XP_006503389.1. XM_006503326.1.
XP_011238899.1. XM_011240597.1.
UniGeneiMm.240619.

3D structure databases

ProteinModelPortaliQ8BKE9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212373. 1 interaction.
STRINGi10090.ENSMUSP00000030311.

PTM databases

iPTMnetiQ8BKE9.
PhosphoSiteiQ8BKE9.

Proteomic databases

MaxQBiQ8BKE9.
PaxDbiQ8BKE9.
PRIDEiQ8BKE9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030311; ENSMUSP00000030311; ENSMUSG00000028576.
GeneIDi67694.
KEGGimmu:67694.
UCSCiuc008tsg.2. mouse.

Organism-specific databases

CTDi80173.
MGIiMGI:1914944. Ift74.

Phylogenomic databases

eggNOGiENOG410IFCX. Eukaryota.
ENOG410XWAZ. LUCA.
GeneTreeiENSGT00390000007109.
HOGENOMiHOG000031494.
HOVERGENiHBG050820.
InParanoidiQ8BKE9.
KOiK19679.
OMAiREENMDA.
OrthoDBiEOG091G05UB.
PhylomeDBiQ8BKE9.
TreeFamiTF318352.

Enzyme and pathway databases

ReactomeiR-MMU-5620924. Intraflagellar transport.

Miscellaneous databases

PROiQ8BKE9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028576.
ExpressionAtlasiQ8BKE9. baseline and differential.
GenevisibleiQ8BKE9. MM.

Family and domain databases

InterProiIPR029602. IFT74.
[Graphical view]
PANTHERiPTHR31432:SF0. PTHR31432:SF0. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiIFT74_MOUSE
AccessioniPrimary (citable) accession number: Q8BKE9
Secondary accession number(s): B1AWG2
, Q80ZI3, Q9CSY1, Q9CUS0, Q9D9T5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 19, 2004
Last modified: September 7, 2016
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.