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Protein

E3 ubiquitin-protein ligase RNF144B

Gene

Rnf144b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death; But, it is not an anti-apoptotic protein per se (By similarity).By similarity

Caution

Lacks the His residue in the RING-type domain 2 that is one of the conserved features of the family.Curated

Catalytic activityi

[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei204By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri30 – 80RING-type 1; atypicalAdd BLAST51
Zinc fingeri101 – 166IBR-typeAdd BLAST66
Zinc fingeri191 – 220RING-type 2; degenerateAdd BLAST30

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processApoptosis, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF144B (EC:2.3.2.31By similarity)
Alternative name(s):
IBR domain-containing protein 2
RING finger protein 144B
Gene namesi
Name:Rnf144b
Synonyms:Ibrdc2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:2384986 Rnf144b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei256 – 276HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000559121 – 301E3 ubiquitin-protein ligase RNF144BAdd BLAST301

Post-translational modificationi

Auto-ubiquitinated.By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ8BKD6
PRIDEiQ8BKD6

PTM databases

PhosphoSitePlusiQ8BKD6

Expressioni

Gene expression databases

BgeeiENSMUSG00000038068
CleanExiMM_RNF144B
GenevisibleiQ8BKD6 MM

Interactioni

Subunit structurei

Interacts with UBE2L3, UBE2L6 and LCMT2 as well as with BAX.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000071017

Structurei

3D structure databases

ProteinModelPortaliQ8BKD6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Members of the RBR family are atypical E3 ligases. They interact with the E2 conjugating enzyme UBE2L3 and function like HECT-type E3 enzymes: they bind E2s via the first RING domain, but require an obligate trans-thiolation step during the ubiquitin transfer, requiring a conserved cysteine residue in the second RING domain.By similarity

Sequence similaritiesi

Belongs to the RBR family. RNF144 subfamily.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri30 – 80RING-type 1; atypicalAdd BLAST51
Zinc fingeri101 – 166IBR-typeAdd BLAST66
Zinc fingeri191 – 220RING-type 2; degenerateAdd BLAST30

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG1815 Eukaryota
ENOG410XP9Y LUCA
GeneTreeiENSGT00840000129738
HOGENOMiHOG000007696
HOVERGENiHBG052072
InParanoidiQ8BKD6
KOiK11975
OMAiVCRIYIE
OrthoDBiEOG091G0FQQ
PhylomeDBiQ8BKD6
TreeFamiTF324777

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR031127 E3_UB_ligase_RBR
IPR002867 IBR_dom
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PANTHERiPTHR11685 PTHR11685, 1 hit
PfamiView protein in Pfam
PF01485 IBR, 2 hits
SMARTiView protein in SMART
SM00647 IBR, 2 hits
SM00184 RING, 2 hits
PROSITEiView protein in PROSITE
PS00518 ZF_RING_1, 1 hit

Sequencei

Sequence statusi: Complete.

Q8BKD6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSVDGLQCL TMTAENPPSG DLIPAPLVTC KLCLCEQSLD KMTMLQECQC
60 70 80 90 100
IFCTPCLKQY MVLSIREGCG SPITCPDMVC LNHGTLQETE IACLVPLDEF
110 120 130 140 150
QLYQRLKFER EVHMDPLRTW CPVADCQTVC HISAGDPGQP VLVECPSCHL
160 170 180 190 200
KFCSCCKDAW HEESSCRDSQ SAMPEHGALF GTDADAPIKQ CPVCRIYIER
210 220 230 240 250
NEGCAQMMCK NCKHTFCWYC LQNLDNDIFL RHYDKGPCRN KLGHSRASVM
260 270 280 290 300
WNRTQVVGIL VGLGVIALVT SPLLLLASPC IICCVCKSCR GKKKKHDPST

T
Length:301
Mass (Da):33,495
Last modified:January 4, 2005 - v2
Checksum:iB31B474C83E7C575
GO

Sequence cautioni

The sequence AAH25007 differs from that shown. Reason: Frameshift at position 147.Curated
The sequence AAH25007 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti174P → T in BAC35416 (PubMed:16141072).Curated1
Sequence conflicti211N → K in BAC35416 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK040939 mRNA Translation: BAC30754.1
AK052846 mRNA Translation: BAC35173.1
AK053529 mRNA Translation: BAC35416.1
BC025007 mRNA Translation: AAH25007.1 Sequence problems.
CCDSiCCDS26491.1
RefSeqiNP_001164114.1, NM_001170643.1
NP_666154.3, NM_146042.4
UniGeneiMm.287609

Genome annotation databases

EnsembliENSMUST00000068891; ENSMUSP00000071017; ENSMUSG00000038068
ENSMUST00000110111; ENSMUSP00000105738; ENSMUSG00000038068
GeneIDi218215
KEGGimmu:218215
UCSCiuc007qhx.2 mouse

Similar proteinsi

Entry informationi

Entry nameiR144B_MOUSE
AccessioniPrimary (citable) accession number: Q8BKD6
Secondary accession number(s): Q8BG97, Q8R195
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: January 4, 2005
Last modified: May 23, 2018
This is version 124 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health