Reviewed, UniProtKB/Swiss-Prot Q8BKC8 (PI4KB_MOUSE)

Names and origin

Protein names

Recommended name:
    Phosphatidylinositol 4-kinase beta
      Short name=PtdIns 4-kinase beta
      Short name=PI4K-beta
      Short name=PI4Kbeta
    EC=2.7.1.67

Gene names

Name:	Pi4kb
Synonyms:	Pik4cb

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	816 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation By similarity. Involved in Golgi-to-plasma membrane trafficking By similarity.
Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate. UniProtKB Q9UBF8
Enzyme regulation	Inhibited by wortmannin By similarity. Increased kinase acitvity upon interaction with FREQ/NCS1 By similarity. UniProtKB Q9UBF8
Subunit structure	Interacts with FREQ/NCS1 in a calcium-independent manner By similarity. Interacts with CALN1/CABP8 and CALN2/CABP7; in a calcium-dependent manner By similarity. This binding competes with FREQ/NCS1 binding By similarity. Interacts with ARF1 and ARF3 By similarity.
Subcellular location	Endomembrane system By similarity. Mitochondrion outer membrane; Peripheral membrane protein By similarity. Rough endoplasmic reticulum membrane; Peripheral membrane protein By similarity. Golgi apparatus By similarity. Note: Found in the outer membrane of mitochondria and membranes of the rough endoplasmic reticulum. Recruited to the Golgi complex by the small GTPase ARF to stimulate the synthesis of phosphatidylinositol 4,5-biphosphate (PIP2) on the Golgi complex By similarity.
Sequence similarities	Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily. Contains 1 PI3K/PI4K domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Golgi apparatus Membrane Mitochondrion Mitochondrion outer membrane
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell endomembrane system Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-phosphatidylinositol 4-kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8BKC8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8BKC8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     304-318: Missing.

Isoform 3 (identifier: Q8BKC8-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-332: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 816	816	Phosphatidylinositol 4-kinase beta		PRO_0000088830
Regions
	Domain	543 – 750	208	PI3K/PI4K
Amino acid modifications
	Modified residue	258	1	Phosphoserine By similarity UniProtKB Q9UBF8
	Modified residue	263	1	Phosphothreonine By similarity UniProtKB Q9UBF8
	Modified residue	266	1	Phosphoserine By similarity UniProtKB Q9UBF8
	Modified residue	277	1	Phosphoserine By similarity UniProtKB Q9UBF8
	Modified residue	280	1	Phosphothreonine By similarity
	Modified residue	294	1	Phosphoserine By similarity UniProtKB Q9UBF8
	Modified residue	428	1	Phosphoserine Ref.4
	Modified residue	438	1	Phosphothreonine By similarity
	Modified residue	511	1	Phosphoserine Ref.4
	Modified residue	517	1	Phosphothreonine By similarity
	Modified residue	519	1	Phosphothreonine By similarity
Natural variations
	Alternative sequence	1 – 332	332	Missing in isoform 3.		VSP_037134
	Alternative sequence	304 – 318	15	Missing in isoform 2.		VSP_037135
Experimental info
	Sequence conflict	20	1	S → T in BAC35448. Ref.1
	Sequence conflict	135	1	E → D in BAE42812. Ref.1
	Sequence conflict	475	1	H → R in BAE42812. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 5, 2009. Version 2. Checksum: F14DC6C055193711	FASTA	816	91,515
	10 20 30 40 50 60 MGDMVVEPAT LKPTSEPTPS PSGNNGGSLL SVITEGVGEL SVIDPEVAQK ACQEVLEKVK 70 80 90 100 110 120 LLHGGVAISS KGTPLELVNG DGVDNEIRCL DDPPAQIREE EDEMGAGVAS GTAKGARRRR 130 140 150 160 170 180 QNNSAKQSWL LRLFESKLFD ISMAISYLYN SKEPGVQAYI GNRLFYFRNE DVDFYLPQLL 190 200 210 220 230 240 NMYIHMDEDV GDAIKPYIVH RCRQSINFSL QCALLLGAYS SDMHISTQRH SRGTKLRKLI 250 260 270 280 290 300 LSDELKPAHR KRELPTLSPA PDTGLSPSKR THQRSKSDAT ASISLSSNLK RTASNPKVEN 310 320 330 340 350 360 EDEELSSSTE SIDNSFSSPV RLAPEREFIK SLMAIGKRLA TLPTKEQKTQ RLISELSLLN 370 380 390 400 410 420 HKLPARVWLP TAGFDHHVVR VPHTQAVVLN SKDKAPYLIY VEVLECENFD TTSVPARIPE 430 440 450 460 470 480 NRIRSTRSVE NLPECGITHE QRAGSFSTVP NYDNDDEAWS VDDIGELQVE LPEVHTNSCD 490 500 510 520 530 540 NISQFSVDSI TSQESKEPVF IAAGDIRRRL SEQLAHTPTA FKRDPEDPSA VALKEPWQEK 550 560 570 580 590 600 VRRIREGSPY GHLPNWRLLS VIVKCGDDLR QELLAFQVLK QLQSIWEQER VPLWIKPYKI 610 620 630 640 650 660 LVISADSGMI EPVVNAVSIH QVKKQSQLSL LDYFLQEHGS YTTEAFLSAQ RNFVQSCAGY 670 680 690 700 710 720 CLVCYLLQVK DRHNGNILLD AEGHIIHIDF GFILSSSPRN LGFETSAFKL TTEFVDVMGG 730 740 750 760 770 780 LNGDMFNYYK MLMLQGLIAA RKHMDKVVQI VEIMQQGSQL PCFHGSSTIR NLKERFHMSM 790 800 810 TEEQLQLLVE QMVDGSMRSI TTKLYDGFQY LTNGIM
	Isoform 2. Checksum: 843762F2E504CA07	FASTA	801	89,944
	10 20 30 40 50 60 MGDMVVEPAT LKPTSEPTPS PSGNNGGSLL SVITEGVGEL SVIDPEVAQK ACQEVLEKVK 70 80 90 100 110 120 LLHGGVAISS KGTPLELVNG DGVDNEIRCL DDPPAQIREE EDEMGAGVAS GTAKGARRRR 130 140 150 160 170 180 QNNSAKQSWL LRLFESKLFD ISMAISYLYN SKEPGVQAYI GNRLFYFRNE DVDFYLPQLL 190 200 210 220 230 240 NMYIHMDEDV GDAIKPYIVH RCRQSINFSL QCALLLGAYS SDMHISTQRH SRGTKLRKLI 250 260 270 280 290 300 LSDELKPAHR KRELPTLSPA PDTGLSPSKR THQRSKSDAT ASISLSSNLK RTASNPKVEN 310 320 330 340 350 360 EDEPVRLAPE REFIKSLMAI GKRLATLPTK EQKTQRLISE LSLLNHKLPA RVWLPTAGFD 370 380 390 400 410 420 HHVVRVPHTQ AVVLNSKDKA PYLIYVEVLE CENFDTTSVP ARIPENRIRS TRSVENLPEC 430 440 450 460 470 480 GITHEQRAGS FSTVPNYDND DEAWSVDDIG ELQVELPEVH TNSCDNISQF SVDSITSQES 490 500 510 520 530 540 KEPVFIAAGD IRRRLSEQLA HTPTAFKRDP EDPSAVALKE PWQEKVRRIR EGSPYGHLPN 550 560 570 580 590 600 WRLLSVIVKC GDDLRQELLA FQVLKQLQSI WEQERVPLWI KPYKILVISA DSGMIEPVVN 610 620 630 640 650 660 AVSIHQVKKQ SQLSLLDYFL QEHGSYTTEA FLSAQRNFVQ SCAGYCLVCY LLQVKDRHNG 670 680 690 700 710 720 NILLDAEGHI IHIDFGFILS SSPRNLGFET SAFKLTTEFV DVMGGLNGDM FNYYKMLMLQ 730 740 750 760 770 780 GLIAARKHMD KVVQIVEIMQ QGSQLPCFHG SSTIRNLKER FHMSMTEEQL QLLVEQMVDG 790 800 SMRSITTKLY DGFQYLTNGI M
	Isoform 3. Checksum: 37547D184F258026	FASTA	484	54,975
	10 20 30 40 50 60 MAIGKRLATL PTKEQKTQRL ISELSLLNHK LPARVWLPTA GFDHHVVRVP HTQAVVLNSK 70 80 90 100 110 120 DKAPYLIYVE VLECENFDTT SVPARIPENR IRSTRSVENL PECGITHEQR AGSFSTVPNY 130 140 150 160 170 180 DNDDEAWSVD DIGELQVELP EVHTNSCDNI SQFSVDSITS QESKEPVFIA AGDIRRRLSE 190 200 210 220 230 240 QLAHTPTAFK RDPEDPSAVA LKEPWQEKVR RIREGSPYGH LPNWRLLSVI VKCGDDLRQE 250 260 270 280 290 300 LLAFQVLKQL QSIWEQERVP LWIKPYKILV ISADSGMIEP VVNAVSIHQV KKQSQLSLLD 310 320 330 340 350 360 YFLQEHGSYT TEAFLSAQRN FVQSCAGYCL VCYLLQVKDR HNGNILLDAE GHIIHIDFGF 370 380 390 400 410 420 ILSSSPRNLG FETSAFKLTT EFVDVMGGLN GDMFNYYKML MLQGLIAARK HMDKVVQIVE 430 440 450 460 470 480 IMQQGSQLPC FHGSSTIRNL KERFHMSMTE EQLQLLVEQM VDGSMRSITT KLYDGFQYLT NGIM

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6J and NOD. Tissue: Eye, Inner ear, Skin and Spleen.
[2]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3). Strain: C57BL/6. Tissue: Embryonic brain.
[4]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed: 18973353] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-428 AND SER-511, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK028974 mRNA. Translation: BAC26222.1. AK053614 mRNA. Translation: BAC35448.1. AK157918 mRNA. Translation: BAE34263.1. AK172074 mRNA. Translation: BAE42812.1. CH466620 Genomic DNA. Translation: EDL38762.1. BC079846 mRNA. Translation: AAH79846.1. BC113176 mRNA. Translation: AAI13177.1. BC113781 mRNA. Translation: AAI13782.1. BC138457 mRNA. Translation: AAI38458.1. BC138458 mRNA. Translation: AAI38459.1.
IPI	IPI00341034. IPI00830658. IPI00929848.
RefSeq	NP_780565.2.
UniGene	Mm.217222
3D structure databases
SMR	Q8BKC8. Positions 560-815.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8BKC8.
PTM databases
PhosphoSite	Q8BKC8.
Proteomic databases
PRIDE	Q8BKC8.
Genome annotation databases
Ensembl	ENSMUST00000107251; ENSMUSP00000102872; ENSMUSG00000038861; Mus musculus. [Genome view]
GeneID	107650.
KEGG	mmu:107650.
UCSC	uc008qhl.1. mouse.
Organism-specific databases
CTD	107650.
MGI	MGI:1334433. Pi4kb.
Phylogenomic databases
eggNOG	roNOG09115.
HOGENOM	HBG389654.
HOVERGEN	Q8BKC8.
OMA	DMVVEPA.
PhylomeDB	Q8BKC8.
Enzyme and pathway databases
BRENDA	2.7.1.67. 244.
Gene expression databases
ArrayExpress	Q8BKC8.
Bgee	Q8BKC8.
Genevestigator	Q8BKC8.
GermOnline	ENSMUSG00000038861. Mus musculus.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000403. PI3/4_kinase_cat. IPR018936. PI3/4_kinase_CS. [Graphical view]
Gene3D	G3DSA:1.10.1070.11. PI3/4_kinase_cat. 1 hit.
Pfam	PF00454. PI3_PI4_kinase. 1 hit. [Graphical view]
SMART	SM00146. PI3Kc. 1 hit. [Graphical view]
PROSITE	PS00915. PI3_4_KINASE_1. 1 hit. PS00916. PI3_4_KINASE_2. 1 hit. PS50290. PI3_4_KINASE_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	359188.
SOURCE	Search...

Entry information

Entry name

PI4KB_MOUSE

Accession

Primary (citable) accession number: Q8BKC8
Secondary accession number(s): Q14CH6 , Q14DJ4, Q3TA58, Q68FH2, Q8C146

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 14, 2003
Last sequence update:	May 5, 2009
Last modified:	January 19, 2010
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)