Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphatidylinositol 4-kinase beta

Gene

Pi4kb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation (By similarity). Involved in Golgi-to-plasma membrane trafficking (By similarity).By similarity

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate.By similarity

Enzyme regulationi

Inhibited by wortmannin. Increased kinase activity upon interaction with NCS1/FREQ.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-1660514 Synthesis of PIPs at the Golgi membrane

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 4-kinase beta (EC:2.7.1.67)
Short name:
PI4K-beta
Short name:
PI4Kbeta
Short name:
PtdIns 4-kinase beta
Gene namesi
Name:Pi4kb
Synonyms:Pik4cb
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1334433 Pi4kb

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000888302 – 816Phosphatidylinositol 4-kinase betaAdd BLAST815

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylglycineBy similarity1
Modified residuei258PhosphoserineBy similarity1
Modified residuei263PhosphothreonineBy similarity1
Modified residuei266PhosphoserineBy similarity1
Modified residuei275PhosphoserineCombined sources1
Modified residuei277PhosphoserineCombined sources1
Modified residuei284PhosphoserineCombined sources1
Modified residuei294PhosphoserineBy similarity1
Modified residuei428PhosphoserineCombined sources1
Modified residuei438PhosphothreonineBy similarity1
Modified residuei511PhosphoserineCombined sources1
Modified residuei517PhosphothreonineBy similarity1
Modified residuei519PhosphothreonineBy similarity1
Isoform 2 (identifier: Q8BKC8-2)
Modified residuei294PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ8BKC8
PeptideAtlasiQ8BKC8
PRIDEiQ8BKC8

PTM databases

iPTMnetiQ8BKC8
PhosphoSitePlusiQ8BKC8

Expressioni

Gene expression databases

BgeeiENSMUSG00000038861
ExpressionAtlasiQ8BKC8 baseline and differential
GenevisibleiQ8BKC8 MM

Interactioni

Subunit structurei

Interacts with ARF1 and ARF3 in the Golgi complex, but not with ARF4, ARF5 or ARF6 (By similarity). Interacts with NCS1/FREQ in a calcium-independent manner. Interacts with CALN1/CABP8 and CALN2/CABP7; in a calcium-dependent manner; this interaction competes with NCS1/FREQ binding (By similarity). Interacts with ACBD3. Interacts with ARMH3, YWHAB, YWHAE, YWHAG, YWHAH, YWHAQ, YWHAZ and SFN (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ8BKC8, 23 interactors
MINTiQ8BKC8
STRINGi10090.ENSMUSP00000072134

Structurei

3D structure databases

ProteinModelPortaliQ8BKC8
SMRiQ8BKC8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini52 – 242PIK helicalPROSITE-ProRule annotationAdd BLAST191
Domaini543 – 750PI3K/PI4KPROSITE-ProRule annotationCuratedAdd BLAST208

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 68Interaction with ACBD3By similarityAdd BLAST67

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0903 Eukaryota
ENOG410XPH3 LUCA
GeneTreeiENSGT00550000074892
HOGENOMiHOG000008289
HOVERGENiHBG053597
InParanoidiQ8BKC8
KOiK19801
TreeFamiTF102042

Family and domain databases

Gene3Di1.10.1070.11, 1 hit
InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000403 PI3/4_kinase_cat_dom
IPR036940 PI3/4_kinase_cat_sf
IPR018936 PI3/4_kinase_CS
IPR015433 PI_Kinase
IPR001263 PInositide-3_kin_accessory_dom
PANTHERiPTHR10048 PTHR10048, 2 hits
PfamiView protein in Pfam
PF00454 PI3_PI4_kinase, 1 hit
SMARTiView protein in SMART
SM00146 PI3Kc, 1 hit
SUPFAMiSSF56112 SSF56112, 2 hits
PROSITEiView protein in PROSITE
PS00915 PI3_4_KINASE_1, 1 hit
PS00916 PI3_4_KINASE_2, 1 hit
PS50290 PI3_4_KINASE_3, 1 hit
PS51545 PIK_HELICAL, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BKC8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGDMVVEPAT LKPTSEPTPS PSGNNGGSLL SVITEGVGEL SVIDPEVAQK
60 70 80 90 100
ACQEVLEKVK LLHGGVAISS KGTPLELVNG DGVDNEIRCL DDPPAQIREE
110 120 130 140 150
EDEMGAGVAS GTAKGARRRR QNNSAKQSWL LRLFESKLFD ISMAISYLYN
160 170 180 190 200
SKEPGVQAYI GNRLFYFRNE DVDFYLPQLL NMYIHMDEDV GDAIKPYIVH
210 220 230 240 250
RCRQSINFSL QCALLLGAYS SDMHISTQRH SRGTKLRKLI LSDELKPAHR
260 270 280 290 300
KRELPTLSPA PDTGLSPSKR THQRSKSDAT ASISLSSNLK RTASNPKVEN
310 320 330 340 350
EDEELSSSTE SIDNSFSSPV RLAPEREFIK SLMAIGKRLA TLPTKEQKTQ
360 370 380 390 400
RLISELSLLN HKLPARVWLP TAGFDHHVVR VPHTQAVVLN SKDKAPYLIY
410 420 430 440 450
VEVLECENFD TTSVPARIPE NRIRSTRSVE NLPECGITHE QRAGSFSTVP
460 470 480 490 500
NYDNDDEAWS VDDIGELQVE LPEVHTNSCD NISQFSVDSI TSQESKEPVF
510 520 530 540 550
IAAGDIRRRL SEQLAHTPTA FKRDPEDPSA VALKEPWQEK VRRIREGSPY
560 570 580 590 600
GHLPNWRLLS VIVKCGDDLR QELLAFQVLK QLQSIWEQER VPLWIKPYKI
610 620 630 640 650
LVISADSGMI EPVVNAVSIH QVKKQSQLSL LDYFLQEHGS YTTEAFLSAQ
660 670 680 690 700
RNFVQSCAGY CLVCYLLQVK DRHNGNILLD AEGHIIHIDF GFILSSSPRN
710 720 730 740 750
LGFETSAFKL TTEFVDVMGG LNGDMFNYYK MLMLQGLIAA RKHMDKVVQI
760 770 780 790 800
VEIMQQGSQL PCFHGSSTIR NLKERFHMSM TEEQLQLLVE QMVDGSMRSI
810
TTKLYDGFQY LTNGIM
Length:816
Mass (Da):91,515
Last modified:May 5, 2009 - v2
Checksum:iF14DC6C055193711
GO
Isoform 2 (identifier: Q8BKC8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     304-318: Missing.

Show »
Length:801
Mass (Da):89,944
Checksum:i843762F2E504CA07
GO
Isoform 3 (identifier: Q8BKC8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-332: Missing.

Show »
Length:484
Mass (Da):54,975
Checksum:i37547D184F258026
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti20S → T in BAC35448 (PubMed:16141072).Curated1
Sequence conflicti135E → D in BAE42812 (PubMed:16141072).Curated1
Sequence conflicti475H → R in BAE42812 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0371341 – 332Missing in isoform 3. 1 PublicationAdd BLAST332
Alternative sequenceiVSP_037135304 – 318Missing in isoform 2. 2 PublicationsAdd BLAST15

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028974 mRNA Translation: BAC26222.1
AK053614 mRNA Translation: BAC35448.1
AK157918 mRNA Translation: BAE34263.1
AK172074 mRNA Translation: BAE42812.1
CH466620 Genomic DNA Translation: EDL38762.1
BC079846 mRNA Translation: AAH79846.1
BC113176 mRNA Translation: AAI13177.1
BC113781 mRNA Translation: AAI13782.1
BC138457 mRNA Translation: AAI38458.1
BC138458 mRNA Translation: AAI38459.1
CCDSiCCDS17599.1 [Q8BKC8-2]
CCDS79978.1 [Q8BKC8-1]
CCDS79979.1 [Q8BKC8-3]
RefSeqiNP_001280644.1, NM_001293715.1 [Q8BKC8-1]
NP_001280645.1, NM_001293716.1 [Q8BKC8-3]
NP_780565.2, NM_175356.3 [Q8BKC8-2]
UniGeneiMm.217222

Genome annotation databases

EnsembliENSMUST00000072287; ENSMUSP00000072134; ENSMUSG00000038861 [Q8BKC8-2]
ENSMUST00000107251; ENSMUSP00000102872; ENSMUSG00000038861 [Q8BKC8-1]
ENSMUST00000167008; ENSMUSP00000132150; ENSMUSG00000038861 [Q8BKC8-3]
GeneIDi107650
KEGGimmu:107650
UCSCiuc008qhl.3 mouse [Q8BKC8-2]
uc008qhm.3 mouse [Q8BKC8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPI4KB_MOUSE
AccessioniPrimary (citable) accession number: Q8BKC8
Secondary accession number(s): Q14CH6
, Q14DJ4, Q3TA58, Q68FH2, Q8C146
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: May 5, 2009
Last modified: June 20, 2018
This is version 137 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health