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Protein

Phosphatidylinositol 4-kinase beta

Gene

Pi4kb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation (By similarity). Involved in Golgi-to-plasma membrane trafficking (By similarity).By similarity

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate.By similarity

Enzyme regulationi

Inhibited by wortmannin. Increased kinase activity upon interaction with NCS1/FREQ.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-1660514. Synthesis of PIPs at the Golgi membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 4-kinase beta (EC:2.7.1.67)
Short name:
PI4K-beta
Short name:
PI4Kbeta
Short name:
PtdIns 4-kinase beta
Gene namesi
Name:Pi4kb
Synonyms:Pik4cb
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1334433. Pi4kb.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 816815Phosphatidylinositol 4-kinase betaPRO_0000088830Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylglycineBy similarity
Modified residuei258 – 2581PhosphoserineBy similarity
Modified residuei263 – 2631PhosphothreonineBy similarity
Modified residuei266 – 2661PhosphoserineBy similarity
Modified residuei275 – 2751PhosphoserineCombined sources
Modified residuei277 – 2771PhosphoserineCombined sources
Modified residuei284 – 2841PhosphoserineCombined sources
Modified residuei294 – 2941PhosphoserineBy similarity
Modified residuei428 – 4281PhosphoserineCombined sources
Modified residuei438 – 4381PhosphothreonineBy similarity
Modified residuei511 – 5111PhosphoserineCombined sources
Modified residuei519 – 5191PhosphothreonineBy similarity
Isoform 2 (identifier: Q8BKC8-2)
Modified residuei294 – 2941PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8BKC8.
MaxQBiQ8BKC8.
PaxDbiQ8BKC8.
PRIDEiQ8BKC8.

PTM databases

iPTMnetiQ8BKC8.
PhosphoSiteiQ8BKC8.

Expressioni

Gene expression databases

BgeeiQ8BKC8.
ExpressionAtlasiQ8BKC8. baseline and differential.
GenevisibleiQ8BKC8. MM.

Interactioni

Subunit structurei

Interacts with ARF1 and ARF3 in the Golgi complex, but not with ARF4, ARF5 or ARF6 (By similarity). Interacts with NCS1/FREQ in a calcium-independent manner. Interacts with CALN1/CABP8 and CALN2/CABP7; in a calcium-dependent manner; this interaction competes with NCS1/FREQ binding (By similarity). Interacts with ACBD3. Interacts with YWHAB, YWHAE, YWHAG, YWHAH, YWHAQ, YWHAZ and SFN (By similarity).By similarity

Protein-protein interaction databases

IntActiQ8BKC8. 23 interactions.
STRINGi10090.ENSMUSP00000072134.

Structurei

3D structure databases

ProteinModelPortaliQ8BKC8.
SMRiQ8BKC8. Positions 130-224, 321-421, 517-799.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini52 – 242191PIK helicalPROSITE-ProRule annotationAdd
BLAST
Domaini543 – 750208PI3K/PI4KPROSITE-ProRule annotationCuratedAdd
BLAST

Sequence similaritiesi

Contains 1 PI3K/PI4K domain.PROSITE-ProRule annotation
Contains 1 PIK helical domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0903. Eukaryota.
ENOG410XPH3. LUCA.
GeneTreeiENSGT00550000074892.
HOGENOMiHOG000008289.
HOVERGENiHBG053597.
InParanoidiQ8BKC8.
KOiK19801.
OrthoDBiEOG773XFQ.
TreeFamiTF102042.

Family and domain databases

Gene3Di1.10.1070.11. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR015433. PI_Kinase.
IPR001263. PInositide-3_kin_accessory_dom.
[Graphical view]
PANTHERiPTHR10048. PTHR10048. 1 hit.
PfamiPF00454. PI3_PI4_kinase. 1 hit.
[Graphical view]
SMARTiSM00146. PI3Kc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
PS51545. PIK_HELICAL. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BKC8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGDMVVEPAT LKPTSEPTPS PSGNNGGSLL SVITEGVGEL SVIDPEVAQK
60 70 80 90 100
ACQEVLEKVK LLHGGVAISS KGTPLELVNG DGVDNEIRCL DDPPAQIREE
110 120 130 140 150
EDEMGAGVAS GTAKGARRRR QNNSAKQSWL LRLFESKLFD ISMAISYLYN
160 170 180 190 200
SKEPGVQAYI GNRLFYFRNE DVDFYLPQLL NMYIHMDEDV GDAIKPYIVH
210 220 230 240 250
RCRQSINFSL QCALLLGAYS SDMHISTQRH SRGTKLRKLI LSDELKPAHR
260 270 280 290 300
KRELPTLSPA PDTGLSPSKR THQRSKSDAT ASISLSSNLK RTASNPKVEN
310 320 330 340 350
EDEELSSSTE SIDNSFSSPV RLAPEREFIK SLMAIGKRLA TLPTKEQKTQ
360 370 380 390 400
RLISELSLLN HKLPARVWLP TAGFDHHVVR VPHTQAVVLN SKDKAPYLIY
410 420 430 440 450
VEVLECENFD TTSVPARIPE NRIRSTRSVE NLPECGITHE QRAGSFSTVP
460 470 480 490 500
NYDNDDEAWS VDDIGELQVE LPEVHTNSCD NISQFSVDSI TSQESKEPVF
510 520 530 540 550
IAAGDIRRRL SEQLAHTPTA FKRDPEDPSA VALKEPWQEK VRRIREGSPY
560 570 580 590 600
GHLPNWRLLS VIVKCGDDLR QELLAFQVLK QLQSIWEQER VPLWIKPYKI
610 620 630 640 650
LVISADSGMI EPVVNAVSIH QVKKQSQLSL LDYFLQEHGS YTTEAFLSAQ
660 670 680 690 700
RNFVQSCAGY CLVCYLLQVK DRHNGNILLD AEGHIIHIDF GFILSSSPRN
710 720 730 740 750
LGFETSAFKL TTEFVDVMGG LNGDMFNYYK MLMLQGLIAA RKHMDKVVQI
760 770 780 790 800
VEIMQQGSQL PCFHGSSTIR NLKERFHMSM TEEQLQLLVE QMVDGSMRSI
810
TTKLYDGFQY LTNGIM
Length:816
Mass (Da):91,515
Last modified:May 5, 2009 - v2
Checksum:iF14DC6C055193711
GO
Isoform 2 (identifier: Q8BKC8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     304-318: Missing.

Show »
Length:801
Mass (Da):89,944
Checksum:i843762F2E504CA07
GO
Isoform 3 (identifier: Q8BKC8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-332: Missing.

Show »
Length:484
Mass (Da):54,975
Checksum:i37547D184F258026
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti20 – 201S → T in BAC35448 (PubMed:16141072).Curated
Sequence conflicti135 – 1351E → D in BAE42812 (PubMed:16141072).Curated
Sequence conflicti475 – 4751H → R in BAE42812 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 332332Missing in isoform 3. 1 PublicationVSP_037134Add
BLAST
Alternative sequencei304 – 31815Missing in isoform 2. 2 PublicationsVSP_037135Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028974 mRNA. Translation: BAC26222.1.
AK053614 mRNA. Translation: BAC35448.1.
AK157918 mRNA. Translation: BAE34263.1.
AK172074 mRNA. Translation: BAE42812.1.
CH466620 Genomic DNA. Translation: EDL38762.1.
BC079846 mRNA. Translation: AAH79846.1.
BC113176 mRNA. Translation: AAI13177.1.
BC113781 mRNA. Translation: AAI13782.1.
BC138457 mRNA. Translation: AAI38458.1.
BC138458 mRNA. Translation: AAI38459.1.
CCDSiCCDS17599.1. [Q8BKC8-2]
CCDS79978.1. [Q8BKC8-1]
CCDS79979.1. [Q8BKC8-3]
RefSeqiNP_001280644.1. NM_001293715.1. [Q8BKC8-1]
NP_001280645.1. NM_001293716.1. [Q8BKC8-3]
NP_780565.2. NM_175356.3. [Q8BKC8-2]
UniGeneiMm.217222.

Genome annotation databases

EnsembliENSMUST00000072287; ENSMUSP00000072134; ENSMUSG00000038861. [Q8BKC8-2]
ENSMUST00000107251; ENSMUSP00000102872; ENSMUSG00000038861. [Q8BKC8-1]
ENSMUST00000167008; ENSMUSP00000132150; ENSMUSG00000038861. [Q8BKC8-3]
GeneIDi107650.
KEGGimmu:107650.
UCSCiuc008qhl.3. mouse. [Q8BKC8-2]
uc008qhm.3. mouse. [Q8BKC8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028974 mRNA. Translation: BAC26222.1.
AK053614 mRNA. Translation: BAC35448.1.
AK157918 mRNA. Translation: BAE34263.1.
AK172074 mRNA. Translation: BAE42812.1.
CH466620 Genomic DNA. Translation: EDL38762.1.
BC079846 mRNA. Translation: AAH79846.1.
BC113176 mRNA. Translation: AAI13177.1.
BC113781 mRNA. Translation: AAI13782.1.
BC138457 mRNA. Translation: AAI38458.1.
BC138458 mRNA. Translation: AAI38459.1.
CCDSiCCDS17599.1. [Q8BKC8-2]
CCDS79978.1. [Q8BKC8-1]
CCDS79979.1. [Q8BKC8-3]
RefSeqiNP_001280644.1. NM_001293715.1. [Q8BKC8-1]
NP_001280645.1. NM_001293716.1. [Q8BKC8-3]
NP_780565.2. NM_175356.3. [Q8BKC8-2]
UniGeneiMm.217222.

3D structure databases

ProteinModelPortaliQ8BKC8.
SMRiQ8BKC8. Positions 130-224, 321-421, 517-799.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BKC8. 23 interactions.
STRINGi10090.ENSMUSP00000072134.

PTM databases

iPTMnetiQ8BKC8.
PhosphoSiteiQ8BKC8.

Proteomic databases

EPDiQ8BKC8.
MaxQBiQ8BKC8.
PaxDbiQ8BKC8.
PRIDEiQ8BKC8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000072287; ENSMUSP00000072134; ENSMUSG00000038861. [Q8BKC8-2]
ENSMUST00000107251; ENSMUSP00000102872; ENSMUSG00000038861. [Q8BKC8-1]
ENSMUST00000167008; ENSMUSP00000132150; ENSMUSG00000038861. [Q8BKC8-3]
GeneIDi107650.
KEGGimmu:107650.
UCSCiuc008qhl.3. mouse. [Q8BKC8-2]
uc008qhm.3. mouse. [Q8BKC8-1]

Organism-specific databases

CTDi5298.
MGIiMGI:1334433. Pi4kb.

Phylogenomic databases

eggNOGiKOG0903. Eukaryota.
ENOG410XPH3. LUCA.
GeneTreeiENSGT00550000074892.
HOGENOMiHOG000008289.
HOVERGENiHBG053597.
InParanoidiQ8BKC8.
KOiK19801.
OrthoDBiEOG773XFQ.
TreeFamiTF102042.

Enzyme and pathway databases

ReactomeiR-MMU-1660514. Synthesis of PIPs at the Golgi membrane.

Miscellaneous databases

ChiTaRSiPi4kb. mouse.
PROiQ8BKC8.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BKC8.
ExpressionAtlasiQ8BKC8. baseline and differential.
GenevisibleiQ8BKC8. MM.

Family and domain databases

Gene3Di1.10.1070.11. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR015433. PI_Kinase.
IPR001263. PInositide-3_kin_accessory_dom.
[Graphical view]
PANTHERiPTHR10048. PTHR10048. 1 hit.
PfamiPF00454. PI3_PI4_kinase. 1 hit.
[Graphical view]
SMARTiSM00146. PI3Kc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
PS51545. PIK_HELICAL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J and NOD.
    Tissue: Eye, Inner ear, Skin and Spleen.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
    Strain: C57BL/6J.
    Tissue: Embryonic brain.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-294 (ISOFORM 2), IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-275; SER-277; SER-284; SER-428 AND SER-511, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-294 (ISOFORM 2), IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiPI4KB_MOUSE
AccessioniPrimary (citable) accession number: Q8BKC8
Secondary accession number(s): Q14CH6
, Q14DJ4, Q3TA58, Q68FH2, Q8C146
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: May 5, 2009
Last modified: June 8, 2016
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.