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Protein

Importin-5

Gene

Ipo5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones.2 Publications

GO - Molecular functioni

GO - Biological processi

  • cellular response to amino acid stimulus Source: UniProtKB
  • NLS-bearing protein import into nucleus Source: UniProtKB
  • positive regulation of protein import into nucleus Source: UniProtKB
  • protein import into nucleus, docking Source: GO_Central
  • protein import into nucleus, translocation Source: GO_Central
  • ribosomal protein import into nucleus Source: MGI
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Importin-5
Short name:
Imp5
Alternative name(s):
Importin subunit beta-3
Karyopherin beta-3
Ran-binding protein 5
Short name:
RanBP5
Gene namesi
Name:Ipo5
Synonyms:Kpnb3, Ranbp5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1917822. Ipo5.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • Golgi apparatus Source: MGI
  • intracellular membrane-bounded organelle Source: MGI
  • membrane Source: MGI
  • nuclear membrane Source: MGI
  • nuclear periphery Source: GO_Central
  • nucleoplasm Source: MGI
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 10971096Importin-5PRO_0000120772Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei827 – 8271PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8BKC5.
MaxQBiQ8BKC5.
PaxDbiQ8BKC5.
PeptideAtlasiQ8BKC5.
PRIDEiQ8BKC5.

PTM databases

iPTMnetiQ8BKC5.
PhosphoSiteiQ8BKC5.
SwissPalmiQ8BKC5.

Expressioni

Gene expression databases

BgeeiQ8BKC5.
GenevisibleiQ8BKC5. MM.

Interactioni

Subunit structurei

Interacts with H2A, H2B, H3 and H4 histones. Binds RPL23A, RPS7 and RPL5 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
WasP703158EBI-643605,EBI-644195

Protein-protein interaction databases

BioGridi214142. 1 interaction.
IntActiQ8BKC5. 4 interactions.
MINTiMINT-1669038.
STRINGi10090.ENSMUSP00000032898.

Structurei

3D structure databases

ProteinModelPortaliQ8BKC5.
SMRiQ8BKC5. Positions 14-607.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati5 – 3834HEAT 1By similarity1 PublicationAdd
BLAST
Domaini28 – 9972Importin N-terminalPROSITE-ProRule annotationAdd
BLAST
Repeati43 – 7735HEAT 2By similarity1 PublicationAdd
BLAST
Repeati95 – 12228HEAT 3By similarity1 PublicationAdd
BLAST
Repeati130 – 15728HEAT 4By similarity1 PublicationAdd
BLAST
Repeati167 – 20135HEAT 5By similarity1 PublicationAdd
BLAST
Repeati210 – 24637HEAT 6By similarity1 PublicationAdd
BLAST
Repeati254 – 28936HEAT 7By similarity1 PublicationAdd
BLAST
Repeati298 – 35053HEAT 8By similarity1 PublicationAdd
BLAST
Repeati352 – 38635HEAT 9By similarity1 PublicationAdd
BLAST
Repeati390 – 43041HEAT 10By similarity1 PublicationAdd
BLAST
Repeati432 – 47241HEAT 11By similarity1 PublicationAdd
BLAST
Repeati475 – 52349HEAT 12By similarity1 PublicationAdd
BLAST
Repeati525 – 56844HEAT 13By similarity1 PublicationAdd
BLAST
Repeati570 – 61546HEAT 14By similarity1 PublicationAdd
BLAST
Repeati617 – 69276HEAT 15By similarity1 PublicationAdd
BLAST
Repeati695 – 73743HEAT 16By similarity1 PublicationAdd
BLAST
Repeati741 – 78040HEAT 17By similarity1 PublicationAdd
BLAST
Repeati787 – 85367HEAT 18By similarity1 PublicationAdd
BLAST
Repeati856 – 89540HEAT 19By similarity1 PublicationAdd
BLAST
Repeati903 – 93533HEAT 20By similarity1 PublicationAdd
BLAST
Repeati943 – 98341HEAT 21By similarity1 PublicationAdd
BLAST
Repeati990 – 102132HEAT 22By similarity1 PublicationAdd
BLAST
Repeati1032 – 106736HEAT 23By similarity1 PublicationAdd
BLAST
Repeati1070 – 109324HEAT 24By similarity1 PublicationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni325 – 37551Ran-GTP bindingBy similarityAdd
BLAST

Sequence similaritiesi

Contains 24 HEAT repeats.By similarity1 Publication
Contains 1 importin N-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2171. Eukaryota.
ENOG410XQAJ. LUCA.
GeneTreeiENSGT00550000074773.
HOGENOMiHOG000209725.
HOVERGENiHBG006156.
InParanoidiQ8BKC5.
OMAiDTNLNNM.
OrthoDBiEOG7Q5HCD.
PhylomeDBiQ8BKC5.
TreeFamiTF300344.

Family and domain databases

Gene3Di1.25.10.10. 3 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000357. HEAT.
IPR001494. Importin-beta_N.
[Graphical view]
PfamiPF02985. HEAT. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.
PROSITEiPS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BKC5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAAAEQQQ FYLLLGNLLS PDNVVRKQAE ETYENIPGRS KITFLLQAIR
60 70 80 90 100
NTTAAEEARQ MAAVLLRRLL SSAFDEVYPA LPSDVQTAIK SELLMIIQME
110 120 130 140 150
TQSSMRKKIC DIAAELARNL IDEDGNNQWP EGLKFLFDSV SSQNMGLREA
160 170 180 190 200
ALHIFWNFPG IFGNQQQHYL DVIKRMLVQC MQDQEHPSIR TLSARATAAF
210 220 230 240 250
ILANEHNVAL FKHFADLLPG FLQAVNDSCY QNDDSVLKSL VEIADTVPKY
260 270 280 290 300
LRPHLEATLQ LSLKLCGDTN LNNMQRQLAL EVIVTLSETA AAMLRKHTSL
310 320 330 340 350
IAQTIPQMLA MMVDLEEDED WANADELEDD DFDSNAVAGE SALDRMACGL
360 370 380 390 400
GGKLVLPMIK EHIMQMLQNP DWKYRHAGLM ALSAIGEGCH QQMEGILNEI
410 420 430 440 450
VNFVLLFLQD PHPRVRYAAC NAVGQMATDF APGFQKKFHE KVIAALLQTM
460 470 480 490 500
EDQGNQRVQA HAAAALINFT EDCPKSLLIP YLDNLVKHLH SIMVLKLQEL
510 520 530 540 550
IQKGTKLVLE QVVTSIASVA DTAEEKFVPY YDLFMPSLKH IVENAVQKEL
560 570 580 590 600
RLLRGKTIEC ISLIGLAVGK EKFMQDASDV MQLLLKTQTD FNDMEDDDPQ
610 620 630 640 650
ISYMISAWAR MCKILGKEFQ QYLPVVMGPL MKTASIKPEV ALLDTQDMEN
660 670 680 690 700
MSDDDGWEFV NLGDQQSFGI KTAGLEEKST ACQMLVCYAK ELKEGFVEYT
710 720 730 740 750
EQVVKLMVPL LKFYFHDGVR VAAAESMPLL LECARVRGPE YLTQMWHFMC
760 770 780 790 800
DALIKAIGTE PDSDVLSEIM HSFAKCIEVM GDGCLNNEHF EELGGILKAK
810 820 830 840 850
LEEHFKNQEL RQVKRQDEDY DEQVEESLQD EDDNDVYILT KVSDILHSIF
860 870 880 890 900
SSYKEKVLPW FEQLLPLIVN LICPQRPWPD RQWGLCIFDD IVEHCSPASF
910 920 930 940 950
KYAEYFISPM LQYVCDNSPE VRQAAAYGLG VMAQFGGDNY RPFCTDALPL
960 970 980 990 1000
LVRVIQAPEA KTKENVNATE NCISAVGKIM KFKPDCVNVE EVLPHWLSWL
1010 1020 1030 1040 1050
PLHEDKEEAV QTFSYLCDLI ESNHPIVLGP NNTNLPKIFS IIAEGEMHEA
1060 1070 1080 1090
IKHEDPCAKR LANVVRQVQT SGGLWTECIA QLSPEQQAAI QELLNSA
Length:1,097
Mass (Da):123,591
Last modified:January 23, 2007 - v3
Checksum:iB3197CFC72F3377D
GO
Isoform 2 (identifier: Q8BKC5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-60: Missing.

Note: No experimental confirmation available.
Show »
Length:1,037
Mass (Da):116,915
Checksum:i85D843A6337BD753
GO

Sequence cautioni

The sequence AAG45965.2 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21A → TA (Ref. 1) Curated
Sequence conflicti397 – 3971L → V in AAG45965 (Ref. 1) Curated
Sequence conflicti800 – 8012KL → NV in AAG45965 (Ref. 1) Curated
Sequence conflicti853 – 8531Y → H in AAH54814 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 6060Missing in isoform 2. 1 PublicationVSP_009658Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF294327 mRNA. Translation: AAG45965.2. Different initiation.
AK053681 mRNA. Translation: BAC35471.1.
AK169664 mRNA. Translation: BAE41286.1.
BC052392 mRNA. Translation: AAH52392.1.
BC054814 mRNA. Translation: AAH54814.1.
CCDSiCCDS37014.1. [Q8BKC5-1]
RefSeqiNP_076068.1. NM_023579.4. [Q8BKC5-1]
XP_006519603.1. XM_006519540.1. [Q8BKC5-2]
UniGeneiMm.221452.

Genome annotation databases

EnsembliENSMUST00000032898; ENSMUSP00000032898; ENSMUSG00000030662. [Q8BKC5-1]
GeneIDi70572.
KEGGimmu:70572.
UCSCiuc007uzz.1. mouse. [Q8BKC5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF294327 mRNA. Translation: AAG45965.2. Different initiation.
AK053681 mRNA. Translation: BAC35471.1.
AK169664 mRNA. Translation: BAE41286.1.
BC052392 mRNA. Translation: AAH52392.1.
BC054814 mRNA. Translation: AAH54814.1.
CCDSiCCDS37014.1. [Q8BKC5-1]
RefSeqiNP_076068.1. NM_023579.4. [Q8BKC5-1]
XP_006519603.1. XM_006519540.1. [Q8BKC5-2]
UniGeneiMm.221452.

3D structure databases

ProteinModelPortaliQ8BKC5.
SMRiQ8BKC5. Positions 14-607.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi214142. 1 interaction.
IntActiQ8BKC5. 4 interactions.
MINTiMINT-1669038.
STRINGi10090.ENSMUSP00000032898.

PTM databases

iPTMnetiQ8BKC5.
PhosphoSiteiQ8BKC5.
SwissPalmiQ8BKC5.

Proteomic databases

EPDiQ8BKC5.
MaxQBiQ8BKC5.
PaxDbiQ8BKC5.
PeptideAtlasiQ8BKC5.
PRIDEiQ8BKC5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032898; ENSMUSP00000032898; ENSMUSG00000030662. [Q8BKC5-1]
GeneIDi70572.
KEGGimmu:70572.
UCSCiuc007uzz.1. mouse. [Q8BKC5-1]

Organism-specific databases

CTDi3843.
MGIiMGI:1917822. Ipo5.

Phylogenomic databases

eggNOGiKOG2171. Eukaryota.
ENOG410XQAJ. LUCA.
GeneTreeiENSGT00550000074773.
HOGENOMiHOG000209725.
HOVERGENiHBG006156.
InParanoidiQ8BKC5.
OMAiDTNLNNM.
OrthoDBiEOG7Q5HCD.
PhylomeDBiQ8BKC5.
TreeFamiTF300344.

Miscellaneous databases

ChiTaRSiIpo5. mouse.
PROiQ8BKC5.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BKC5.
GenevisibleiQ8BKC5. MM.

Family and domain databases

Gene3Di1.25.10.10. 3 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000357. HEAT.
IPR001494. Importin-beta_N.
[Graphical view]
PfamiPF02985. HEAT. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.
PROSITEiPS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of testicular germ cell gene expression by differential display analysis."
    Anway M.D., Li Y., Griswold M.D.
    Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: BALB/cJ.
    Tissue: Testis.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J and NOD.
    Tissue: Eye and Thymus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Brain.
  4. "Multiple pathways contribute to nuclear import of core histones."
    Muehlhaeusser P., Mueller E.-C., Otto A., Kutay U.
    EMBO Rep. 2:690-696(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH HISTONES H2B; H2A; H3 AND H4.
  5. Cited for: FUNCTION, SUBCELLULAR LOCATION.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-827, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
  7. "Structural basis for cell-cycle-dependent nuclear import mediated by the karyopherin Kap121p."
    Kobayashi J., Matsuura Y.
    J. Mol. Biol. 425:1852-1868(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: REPEAT STRUCTURE.

Entry informationi

Entry nameiIPO5_MOUSE
AccessioniPrimary (citable) accession number: Q8BKC5
Secondary accession number(s): Q3TEG2, Q7TQC6, Q9EQ30
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 124 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.