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Protein

Pyrethroid hydrolase Ces2e

Gene

Ces2e

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Carboxylesterase that catalyzes the hydrolysis of pyrethroids pesticides. Hydrolyzes trans-permethrin at a rate about 22-fold higher than cis-permethrin. Also hydrolyzes trans-cypermethrin.1 Publication

Catalytic activityi

Trans-permethrin + H2O = (3-phenoxyphenyl)methanol + (1S,3R)-3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropanecarboxylate.1 Publication

Kineticsi

kcat is 94 sec(-1) with p-Nitrophenyl acetate as substrate. kcat is 0.12 sec(-1) with cypermethrin as substrate. kcat is 0.11 sec(-1) with (R/S)-alpha-cyano(6-methoxy-2-naphthyl)-methyl-(R/S)-trans/cis-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane carboxylate as substrate. kcat is 0.067 sec(-1) with (R/S)-alpha-cyano(6-methoxy-2-naphthyl)-methyl-(R)---2-(4-chlorophenyl)-3-methyl butanoate ((alphaR/S)(2R)-A3) as substrate. kcat is 0.099 sec(-1) with chrysanthemic acid as substrate.

  1. KM=286 µM for p-Nitrophenyl acetate1 Publication
  2. KM=0.5 µM for cypermethrin1 Publication
  3. KM=2.2 µM for (R/S)-alpha-cyano(6-methoxy-2-naphthyl)-methyl-(R/S)-trans/cis-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane carboxylate1 Publication
  4. KM=0.89 µM for (R/S)-alpha-cyano(6-methoxy-2-naphthyl)-methyl-(R)---2-(4-chlorophenyl)-3-methyl butanoate ((alphaR/S)(2R)-A3)1 Publication
  5. KM=0.96 µM for chrysanthemic acid1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei228 – 2281Acyl-ester intermediatePROSITE-ProRule annotation
    Active sitei345 – 3451Charge relay systemBy similarity
    Active sitei457 – 4571Charge relay systemBy similarity

    GO - Molecular functioni

    • carboxylic ester hydrolase activity Source: MGI
    • trans-permethrin hydrolase activity Source: UniProtKB-EC
    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase, Serine esterase

    Enzyme and pathway databases

    BRENDAi3.1.1.1. 3474.
    ReactomeiR-MMU-211945. Phase 1 - Functionalization of compounds.

    Protein family/group databases

    ESTHERimouse-Ces2e. Carb_B_Chordata.
    MEROPSiS09.998.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyrethroid hydrolase Ces2e (EC:3.1.1.88)
    Alternative name(s):
    carboxylesterase 2E
    Gene namesi
    Name:Ces2e
    Synonyms:Ces5
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    Proteomesi
    • UP000000589 Componenti: Chromosome 8

    Organism-specific databases

    MGIiMGI:2443170. Ces2e.

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Endoplasmic reticulum, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 26261 PublicationAdd
    BLAST
    Chaini27 – 559533Pyrethroid hydrolase Ces2ePRO_0000424211Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei27 – 271Pyrrolidone carboxylic acidBy similarity
    Disulfide bondi95 ↔ 123By similarity
    Glycosylationi276 – 2761N-linked (GlcNAc...)Sequence analysis
    Disulfide bondi280 ↔ 291By similarity
    Modified residuei552 – 5521PhosphoserineCombined sources

    Post-translational modificationi

    Glycosylated.1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein, Pyrrolidone carboxylic acid

    Proteomic databases

    MaxQBiQ8BK48.
    PaxDbiQ8BK48.
    PeptideAtlasiQ8BK48.
    PRIDEiQ8BK48.

    PTM databases

    iPTMnetiQ8BK48.
    PhosphoSiteiQ8BK48.

    Expressioni

    Gene expression databases

    BgeeiENSMUSG00000031886.
    GenevisibleiQ8BK48. MM.

    Interactioni

    Protein-protein interaction databases

    IntActiQ8BK48. 3 interactions.
    STRINGi10090.ENSMUSP00000034355.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BK48.
    SMRiQ8BK48. Positions 32-539.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiKOG1516. Eukaryota.
    COG2272. LUCA.
    GeneTreeiENSGT00760000118946.
    HOGENOMiHOG000091866.
    HOVERGENiHBG008839.
    InParanoidiQ8BK48.
    KOiK03927.
    OMAiACYLMAS.
    OrthoDBiEOG091G03ZC.
    PhylomeDBiQ8BK48.
    TreeFamiTF315470.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR002018. CarbesteraseB.
    IPR019826. Carboxylesterase_B_AS.
    IPR019819. Carboxylesterase_B_CS.
    [Graphical view]
    PfamiPF00135. COesterase. 1 hit.
    [Graphical view]
    SUPFAMiSSF53474. SSF53474. 1 hit.
    PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
    PS00941. CARBOXYLESTERASE_B_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8BK48-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MPLYKLLGWL NAVACGVLLL VLHVQGQDSA SPIRNTHTGQ VRGSLVHVKD
    60 70 80 90 100
    TDIAVHTFLG IPFAKPPVGP LRFAPPEAPE PWSGVRDGTS HPNMCLQNDN
    110 120 130 140 150
    LMGSEDLKMM NLILPPISMS EDCLYLNIYV PAHAHEGSNL PVMVWIHGGA
    160 170 180 190 200
    LTVGMASMYD GSMLAATEDV VVVAIQYRLG VLGFFSTGDQ HAKGNWGYLD
    210 220 230 240 250
    QVAALRWVQQ NIVHFGGNPD RVTIFGESAG GTSVSSHVVS PMSQGLFHGA
    260 270 280 290 300
    IMESGVAVLP DLISSSSEMV HRIVANLSGC AAVNSETLMC CLRGKNEAEM
    310 320 330 340 350
    LAINKVFKII PGVVDGEFLP KHPQELMASK DFHPVPSIIG INNDEYGWIL
    360 370 380 390 400
    PTIMDPAQKI EEITRKTLPA VLKSTALKMM LPPECGDLLM EEYMGDTEDP
    410 420 430 440 450
    ETLQAQFREM KGDFMFVIPA LQVAHFQRSH APVYFYEFQH RPSFFKDFRP
    460 470 480 490 500
    PYVKADHGDE IFLVFGYQFG NIKLPYTEEE EQLSRRIMKY WANFARHGNP
    510 520 530 540 550
    NSEGLPYWPV MDHDEQYLQL DIQPSVGRAL KARRLQFWTK TLPQKIQELK

    GSQERHKEL
    Length:559
    Mass (Da):62,318
    Last modified:March 1, 2003 - v1
    Checksum:iCC14C61034A122C3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti102 – 1021M → F AA sequence (PubMed:15123619).Curated
    Sequence conflicti487 – 4871I → M in BAC28361 (PubMed:16141072).Curated
    Sequence conflicti491 – 4911W → R in BAC28361 (PubMed:16141072).Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AK033563 mRNA. Translation: BAC28361.1.
    AK077248 mRNA. Translation: BAC36707.1.
    AC166833 Genomic DNA. No translation available.
    CH466525 Genomic DNA. Translation: EDL11239.1.
    BC022148 mRNA. Translation: AAH22148.1.
    BC055062 mRNA. Translation: AAH55062.1.
    CCDSiCCDS22588.1.
    RefSeqiNP_001157228.1. NM_001163756.1.
    NP_766347.1. NM_172759.3.
    UniGeneiMm.441134.

    Genome annotation databases

    EnsembliENSMUST00000034355; ENSMUSP00000034355; ENSMUSG00000031886.
    ENSMUST00000109410; ENSMUSP00000105037; ENSMUSG00000031886.
    GeneIDi234673.
    KEGGimmu:234673.
    UCSCiuc009nbg.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AK033563 mRNA. Translation: BAC28361.1.
    AK077248 mRNA. Translation: BAC36707.1.
    AC166833 Genomic DNA. No translation available.
    CH466525 Genomic DNA. Translation: EDL11239.1.
    BC022148 mRNA. Translation: AAH22148.1.
    BC055062 mRNA. Translation: AAH55062.1.
    CCDSiCCDS22588.1.
    RefSeqiNP_001157228.1. NM_001163756.1.
    NP_766347.1. NM_172759.3.
    UniGeneiMm.441134.

    3D structure databases

    ProteinModelPortaliQ8BK48.
    SMRiQ8BK48. Positions 32-539.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    IntActiQ8BK48. 3 interactions.
    STRINGi10090.ENSMUSP00000034355.

    Protein family/group databases

    ESTHERimouse-Ces2e. Carb_B_Chordata.
    MEROPSiS09.998.

    PTM databases

    iPTMnetiQ8BK48.
    PhosphoSiteiQ8BK48.

    Proteomic databases

    MaxQBiQ8BK48.
    PaxDbiQ8BK48.
    PeptideAtlasiQ8BK48.
    PRIDEiQ8BK48.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSMUST00000034355; ENSMUSP00000034355; ENSMUSG00000031886.
    ENSMUST00000109410; ENSMUSP00000105037; ENSMUSG00000031886.
    GeneIDi234673.
    KEGGimmu:234673.
    UCSCiuc009nbg.2. mouse.

    Organism-specific databases

    CTDi234673.
    MGIiMGI:2443170. Ces2e.

    Phylogenomic databases

    eggNOGiKOG1516. Eukaryota.
    COG2272. LUCA.
    GeneTreeiENSGT00760000118946.
    HOGENOMiHOG000091866.
    HOVERGENiHBG008839.
    InParanoidiQ8BK48.
    KOiK03927.
    OMAiACYLMAS.
    OrthoDBiEOG091G03ZC.
    PhylomeDBiQ8BK48.
    TreeFamiTF315470.

    Enzyme and pathway databases

    BRENDAi3.1.1.1. 3474.
    ReactomeiR-MMU-211945. Phase 1 - Functionalization of compounds.

    Miscellaneous databases

    ChiTaRSiCes5a. mouse.
    PROiQ8BK48.
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSMUSG00000031886.
    GenevisibleiQ8BK48. MM.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR002018. CarbesteraseB.
    IPR019826. Carboxylesterase_B_AS.
    IPR019819. Carboxylesterase_B_CS.
    [Graphical view]
    PfamiPF00135. COesterase. 1 hit.
    [Graphical view]
    SUPFAMiSSF53474. SSF53474. 1 hit.
    PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
    PS00941. CARBOXYLESTERASE_B_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiEST2E_MOUSE
    AccessioniPrimary (citable) accession number: Q8BK48
    Secondary accession number(s): Q8BM97, Q8VC02
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 16, 2013
    Last sequence update: March 1, 2003
    Last modified: September 7, 2016
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.