Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pyrethroid hydrolase Ces2e

Gene

Ces2e

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Carboxylesterase that catalyzes the hydrolysis of pyrethroids pesticides. Hydrolyzes trans-permethrin at a rate about 22-fold higher than cis-permethrin. Also hydrolyzes trans-cypermethrin.1 Publication

Catalytic activityi

Trans-permethrin + H2O = (3-phenoxyphenyl)methanol + (1S,3R)-3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropanecarboxylate.1 Publication

Kineticsi

kcat is 94 sec(-1) with p-Nitrophenyl acetate as substrate. kcat is 0.12 sec(-1) with cypermethrin as substrate. kcat is 0.11 sec(-1) with (R/S)-alpha-cyano(6-methoxy-2-naphthyl)-methyl-(R/S)-trans/cis-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane carboxylate as substrate. kcat is 0.067 sec(-1) with (R/S)-alpha-cyano(6-methoxy-2-naphthyl)-methyl-(R)---2-(4-chlorophenyl)-3-methyl butanoate ((alphaR/S)(2R)-A3) as substrate. kcat is 0.099 sec(-1) with chrysanthemic acid as substrate.
  1. KM=286 µM for p-Nitrophenyl acetate1 Publication
  2. KM=0.5 µM for cypermethrin1 Publication
  3. KM=2.2 µM for (R/S)-alpha-cyano(6-methoxy-2-naphthyl)-methyl-(R/S)-trans/cis-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane carboxylate1 Publication
  4. KM=0.89 µM for (R/S)-alpha-cyano(6-methoxy-2-naphthyl)-methyl-(R)---2-(4-chlorophenyl)-3-methyl butanoate ((alphaR/S)(2R)-A3)1 Publication
  5. KM=0.96 µM for chrysanthemic acid1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei228Acyl-ester intermediatePROSITE-ProRule annotation1
    Active sitei345Charge relay systemBy similarity1
    Active sitei457Charge relay systemBy similarity1

    GO - Molecular functioni

    • carboxylic ester hydrolase activity Source: MGI
    • trans-permethrin hydrolase activity Source: UniProtKB-EC

    Keywordsi

    Molecular functionHydrolase, Serine esterase

    Enzyme and pathway databases

    BRENDAi3.1.1.1 3474
    ReactomeiR-MMU-211945 Phase I - Functionalization of compounds

    Protein family/group databases

    ESTHERimouse-Ces2e Carb_B_Chordata
    MEROPSiS09.998

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyrethroid hydrolase Ces2e (EC:3.1.1.88)
    Alternative name(s):
    carboxylesterase 2E
    Gene namesi
    Name:Ces2e
    Synonyms:Ces5
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
    Proteomesi
    • UP000000589 Componenti: Chromosome 8

    Organism-specific databases

    MGIiMGI:2443170 Ces2e

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Endoplasmic reticulum, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 261 PublicationAdd BLAST26
    ChainiPRO_000042421127 – 559Pyrethroid hydrolase Ces2eAdd BLAST533

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei27Pyrrolidone carboxylic acidBy similarity1
    Disulfide bondi95 ↔ 123By similarity
    Glycosylationi276N-linked (GlcNAc...) asparagineSequence analysis1
    Disulfide bondi280 ↔ 291By similarity
    Modified residuei552PhosphoserineCombined sources1

    Post-translational modificationi

    Glycosylated.1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein, Pyrrolidone carboxylic acid

    Proteomic databases

    MaxQBiQ8BK48
    PaxDbiQ8BK48
    PeptideAtlasiQ8BK48
    PRIDEiQ8BK48

    PTM databases

    iPTMnetiQ8BK48
    PhosphoSitePlusiQ8BK48

    Expressioni

    Gene expression databases

    BgeeiENSMUSG00000031886
    GenevisibleiQ8BK48 MM

    Interactioni

    Protein-protein interaction databases

    IntActiQ8BK48, 3 interactors
    MINTiQ8BK48
    STRINGi10090.ENSMUSP00000034355

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BK48
    SMRiQ8BK48
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiKOG1516 Eukaryota
    COG2272 LUCA
    GeneTreeiENSGT00760000118946
    HOGENOMiHOG000091866
    HOVERGENiHBG008839
    InParanoidiQ8BK48
    KOiK03927
    OMAiIMESAPI
    OrthoDBiEOG091G03ZC
    PhylomeDBiQ8BK48
    TreeFamiTF315470

    Family and domain databases

    Gene3Di3.40.50.1820, 1 hit
    InterProiView protein in InterPro
    IPR029058 AB_hydrolase
    IPR002018 CarbesteraseB
    IPR019826 Carboxylesterase_B_AS
    IPR019819 Carboxylesterase_B_CS
    PfamiView protein in Pfam
    PF00135 COesterase, 1 hit
    SUPFAMiSSF53474 SSF53474, 1 hit
    PROSITEiView protein in PROSITE
    PS00122 CARBOXYLESTERASE_B_1, 1 hit
    PS00941 CARBOXYLESTERASE_B_2, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8BK48-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MPLYKLLGWL NAVACGVLLL VLHVQGQDSA SPIRNTHTGQ VRGSLVHVKD
    60 70 80 90 100
    TDIAVHTFLG IPFAKPPVGP LRFAPPEAPE PWSGVRDGTS HPNMCLQNDN
    110 120 130 140 150
    LMGSEDLKMM NLILPPISMS EDCLYLNIYV PAHAHEGSNL PVMVWIHGGA
    160 170 180 190 200
    LTVGMASMYD GSMLAATEDV VVVAIQYRLG VLGFFSTGDQ HAKGNWGYLD
    210 220 230 240 250
    QVAALRWVQQ NIVHFGGNPD RVTIFGESAG GTSVSSHVVS PMSQGLFHGA
    260 270 280 290 300
    IMESGVAVLP DLISSSSEMV HRIVANLSGC AAVNSETLMC CLRGKNEAEM
    310 320 330 340 350
    LAINKVFKII PGVVDGEFLP KHPQELMASK DFHPVPSIIG INNDEYGWIL
    360 370 380 390 400
    PTIMDPAQKI EEITRKTLPA VLKSTALKMM LPPECGDLLM EEYMGDTEDP
    410 420 430 440 450
    ETLQAQFREM KGDFMFVIPA LQVAHFQRSH APVYFYEFQH RPSFFKDFRP
    460 470 480 490 500
    PYVKADHGDE IFLVFGYQFG NIKLPYTEEE EQLSRRIMKY WANFARHGNP
    510 520 530 540 550
    NSEGLPYWPV MDHDEQYLQL DIQPSVGRAL KARRLQFWTK TLPQKIQELK

    GSQERHKEL
    Length:559
    Mass (Da):62,318
    Last modified:March 1, 2003 - v1
    Checksum:iCC14C61034A122C3
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti102M → F AA sequence (PubMed:15123619).Curated1
    Sequence conflicti487I → M in BAC28361 (PubMed:16141072).Curated1
    Sequence conflicti491W → R in BAC28361 (PubMed:16141072).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AK033563 mRNA Translation: BAC28361.1
    AK077248 mRNA Translation: BAC36707.1
    AC166833 Genomic DNA No translation available.
    CH466525 Genomic DNA Translation: EDL11239.1
    BC022148 mRNA Translation: AAH22148.1
    BC055062 mRNA Translation: AAH55062.1
    CCDSiCCDS22588.1
    RefSeqiNP_001157228.1, NM_001163756.1
    NP_766347.1, NM_172759.3
    UniGeneiMm.441134

    Genome annotation databases

    EnsembliENSMUST00000034355; ENSMUSP00000034355; ENSMUSG00000031886
    ENSMUST00000109410; ENSMUSP00000105037; ENSMUSG00000031886
    GeneIDi234673
    KEGGimmu:234673
    UCSCiuc009nbg.2 mouse

    Similar proteinsi

    Entry informationi

    Entry nameiEST2E_MOUSE
    AccessioniPrimary (citable) accession number: Q8BK48
    Secondary accession number(s): Q8BM97, Q8VC02
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 16, 2013
    Last sequence update: March 1, 2003
    Last modified: March 28, 2018
    This is version 129 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health