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Protein

F-box only protein 9

Gene

Fbxo9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of TTI1 and TELO2 in a CK2-dependent manner, thereby directly regulating mTOR signaling. SCF(FBXO9) recognizes and binds mTORC1-bound TTI1 and TELO2 when they are phosphorylated by CK2 following growth factor deprivation, leading to their degradation. In contrast, the SCF(FBXO9) does not mediate ubiquitination of TTI1 and TELO2 when they are part of the mTORC2 complex. As a consequence, mTORC1 is inactivated to restrain cell growth and protein translation, while mTORC2 is activated due to the relief of feedback inhibition by mTORC1 (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

  • fat cell differentiation Source: MGI
  • innate immune response Source: MGI
  • protein ubiquitination Source: UniProtKB
  • regulation of TOR signaling Source: UniProtKB
  • SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
F-box only protein 9
Gene namesi
Name:Fbxo9
ORF Names:MNCb-2471
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1918788. Fbxo9.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001198871 – 437F-box only protein 9Add BLAST437
Isoform 2 (identifier: Q8BK06-2)
Initiator methionineiRemovedCombined sources
Isoform 3 (identifier: Q8BK06-3)
Initiator methionineiRemovedCombined sources

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei126PhosphoserineCombined sources1
Isoform 2 (identifier: Q8BK06-2)
Modified residuei2N-acetylalanineCombined sources1
Modified residuei10PhosphoserineCombined sources1
Isoform 3 (identifier: Q8BK06-3)
Modified residuei2N-acetylalanineCombined sources1
Modified residuei10PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ8BK06.
PRIDEiQ8BK06.

PTM databases

iPTMnetiQ8BK06.
PhosphoSitePlusiQ8BK06.

Expressioni

Gene expression databases

BgeeiENSMUSG00000001366.
CleanExiMM_FBXO9.
ExpressionAtlasiQ8BK06. baseline and differential.
GenevisibleiQ8BK06. MM.

Interactioni

Subunit structurei

Part of the SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase complex SCF(FBXO9) composed of CUL1, SKP1, RBX1 and FBXO9. Interacts with TTI1 and TELO2; when TTI1 and TELO2 are phosphorylated by CK2 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000001402.

Structurei

3D structure databases

ProteinModelPortaliQ8BK06.
SMRiQ8BK06.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati84 – 117TPRAdd BLAST34
Domaini175 – 226F-boxPROSITE-ProRule annotationAdd BLAST52

Sequence similaritiesi

Contains 1 F-box domain.PROSITE-ProRule annotation
Contains 1 TPR repeat.Curated

Keywords - Domaini

TPR repeat

Phylogenomic databases

eggNOGiKOG2997. Eukaryota.
ENOG410XPVI. LUCA.
GeneTreeiENSGT00390000014256.
HOGENOMiHOG000047162.
HOVERGENiHBG001041.
InParanoidiQ8BK06.
KOiK10295.
OMAiLVWIHHS.
OrthoDBiEOG091G0BDF.
PhylomeDBiQ8BK06.
TreeFamiTF324797.

Family and domain databases

Gene3Di1.20.58.280. 1 hit.
InterProiIPR001810. F-box_dom.
IPR007330. MIT.
[Graphical view]
PfamiPF12937. F-box-like. 1 hit.
[Graphical view]
SUPFAMiSSF116846. SSF116846. 1 hit.
SSF81383. SSF81383. 1 hit.
PROSITEiPS50181. FBOX. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BK06-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSAEAEEDCH SDADRVGDEG NESPAERDLQ AQLQMFRAQW MFELTPGVGS
60 70 80 90 100
SHGETRPCRA GRSSMLKAAA DTKGRQELAK EEKARELFLQ AVEEEQNGAL
110 120 130 140 150
YEAIKFYRRA MQLVPDIEFK ITYTRSPDGD GVGSGYIEEN EDASKMADLL
160 170 180 190 200
SYFQQQLTLQ ESVLKLCQPE LETSQTHISV LPMEVLMYIF RWVVSSDLDL
210 220 230 240 250
RSLEQLSLVC RGFYICARDP EIWRLACLKV WGRSCMKLVP YASWREMFLE
260 270 280 290 300
RPRVRFDGVY ISKTTYIRQG EQSLDGFYRA WHQVEYYRYM RFFPDGHVMM
310 320 330 340 350
LTTPEEPPSI VPRLRTRNTR TDAILLGHYR LSQDADNQTK VFAVITKKKE
360 370 380 390 400
EKPLDHKYRY FRRVPVQEAD HSFHVGLQLC SSGHQRFNKL IWIHHSCHIT
410 420 430
YKATGETAVS AFEIDKMYTP LLFARVRSYT AFSERPL
Note: No experimental confirmation available.
Length:437
Mass (Da):50,760
Last modified:March 1, 2003 - v1
Checksum:i8950C6F658F61FCB
GO
Isoform 2 (identifier: Q8BK06-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-2: MS → M

Show »
Length:436
Mass (Da):50,673
Checksum:i3479C6C144A26805
GO
Isoform 3 (identifier: Q8BK06-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-2: MS → M
     180-195: VLPMEVLMYIFRWVVS → GVAPWLLSLRNPWTSG
     196-437: Missing.

Note: No experimental confirmation available.Combined sources
Show »
Length:194
Mass (Da):21,721
Checksum:i64D572335E85D415
GO

Sequence cautioni

The sequence BAA95068 differs from that shown. Reason: Frameshift at position 228.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti42F → C in BAB31231 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0129291 – 2MS → M in isoform 2 and isoform 3. 3 Publications2
Alternative sequenceiVSP_012930180 – 195VLPME…RWVVS → GVAPWLLSLRNPWTSG in isoform 3. 1 PublicationAdd BLAST16
Alternative sequenceiVSP_012931196 – 437Missing in isoform 3. 1 PublicationAdd BLAST242

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB041585 mRNA. Translation: BAA95068.1. Frameshift.
AK077607 mRNA. Translation: BAC36897.1.
AK018482 mRNA. Translation: BAB31231.1.
BC020074 mRNA. Translation: AAH20074.1.
CCDSiCCDS52863.1. [Q8BK06-2]
CCDS52864.1. [Q8BK06-1]
RefSeqiNP_001074959.1. NM_001081490.2. [Q8BK06-1]
NP_076094.2. NM_023605.2. [Q8BK06-2]
UniGeneiMm.28584.

Genome annotation databases

EnsembliENSMUST00000001402; ENSMUSP00000001402; ENSMUSG00000001366. [Q8BK06-1]
ENSMUST00000085311; ENSMUSP00000082419; ENSMUSG00000001366. [Q8BK06-2]
ENSMUST00000159099; ENSMUSP00000125381; ENSMUSG00000001366. [Q8BK06-3]
GeneIDi71538.
KEGGimmu:71538.
UCSCiuc033jlp.1. mouse. [Q8BK06-2]
uc033jlr.1. mouse. [Q8BK06-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB041585 mRNA. Translation: BAA95068.1. Frameshift.
AK077607 mRNA. Translation: BAC36897.1.
AK018482 mRNA. Translation: BAB31231.1.
BC020074 mRNA. Translation: AAH20074.1.
CCDSiCCDS52863.1. [Q8BK06-2]
CCDS52864.1. [Q8BK06-1]
RefSeqiNP_001074959.1. NM_001081490.2. [Q8BK06-1]
NP_076094.2. NM_023605.2. [Q8BK06-2]
UniGeneiMm.28584.

3D structure databases

ProteinModelPortaliQ8BK06.
SMRiQ8BK06.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000001402.

PTM databases

iPTMnetiQ8BK06.
PhosphoSitePlusiQ8BK06.

Proteomic databases

PaxDbiQ8BK06.
PRIDEiQ8BK06.

Protocols and materials databases

DNASUi71538.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000001402; ENSMUSP00000001402; ENSMUSG00000001366. [Q8BK06-1]
ENSMUST00000085311; ENSMUSP00000082419; ENSMUSG00000001366. [Q8BK06-2]
ENSMUST00000159099; ENSMUSP00000125381; ENSMUSG00000001366. [Q8BK06-3]
GeneIDi71538.
KEGGimmu:71538.
UCSCiuc033jlp.1. mouse. [Q8BK06-2]
uc033jlr.1. mouse. [Q8BK06-1]

Organism-specific databases

CTDi26268.
MGIiMGI:1918788. Fbxo9.

Phylogenomic databases

eggNOGiKOG2997. Eukaryota.
ENOG410XPVI. LUCA.
GeneTreeiENSGT00390000014256.
HOGENOMiHOG000047162.
HOVERGENiHBG001041.
InParanoidiQ8BK06.
KOiK10295.
OMAiLVWIHHS.
OrthoDBiEOG091G0BDF.
PhylomeDBiQ8BK06.
TreeFamiTF324797.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

ChiTaRSiFbxo9. mouse.
PROiQ8BK06.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000001366.
CleanExiMM_FBXO9.
ExpressionAtlasiQ8BK06. baseline and differential.
GenevisibleiQ8BK06. MM.

Family and domain databases

Gene3Di1.20.58.280. 1 hit.
InterProiIPR001810. F-box_dom.
IPR007330. MIT.
[Graphical view]
PfamiPF12937. F-box-like. 1 hit.
[Graphical view]
SUPFAMiSSF116846. SSF116846. 1 hit.
SSF81383. SSF81383. 1 hit.
PROSITEiPS50181. FBOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFBX9_MOUSE
AccessioniPrimary (citable) accession number: Q8BK06
Secondary accession number(s): Q8VDY6, Q9D349, Q9JJC5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.