UniProtKB - Q8BJW7 (EME1_MOUSE)
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Protein
Crossover junction endonuclease EME1
Gene
Eme1
Organism
Mus musculus (Mouse)
Status
Functioni
Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.1 Publication
Cofactori
GO - Molecular functioni
- DNA binding Source: InterPro
- endonuclease activity Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- double-strand break repair Source: GO_Central
- intra-S DNA damage checkpoint Source: GO_Central
- replication fork processing Source: GO_Central
- resolution of meiotic recombination intermediates Source: GO_Central
- response to intra-S DNA damage checkpoint signaling Source: MGI
Keywordsi
Molecular function | Endonuclease, Hydrolase, Nuclease |
Biological process | DNA damage, DNA recombination, DNA repair |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-5693568. Resolution of D-loop Structures through Holliday Junction Intermediates. R-MMU-6783310. Fanconi Anemia Pathway. |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Eme1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:3576783. Eme1. |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000223631 | 1 – 570 | Crossover junction endonuclease EME1Add BLAST | 570 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 12 | PhosphoserineBy similarity | 1 | |
Modified residuei | 15 | PhosphoserineBy similarity | 1 | |
Modified residuei | 84 | PhosphoserineBy similarity | 1 | |
Modified residuei | 85 | PhosphoserineBy similarity | 1 | |
Modified residuei | 87 | PhosphoserineBy similarity | 1 | |
Cross-linki | 103 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 117 | PhosphoserineBy similarity | 1 | |
Cross-linki | 136 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 142 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q8BJW7. |
MaxQBi | Q8BJW7. |
PaxDbi | Q8BJW7. |
PeptideAtlasi | Q8BJW7. |
PRIDEi | Q8BJW7. |
PTM databases
iPTMneti | Q8BJW7. |
PhosphoSitePlusi | Q8BJW7. |
Expressioni
Tissue specificityi
Weakly expressed in brain, heart, kidney, liver, lung, muscle, skin, small intestine, spleen, stomach, testis and thymus (PubMed:14609959, PubMed:27010503). Expressed in bone marrow (PubMed:27010503). Also expressed in embryonic stem cells (ES cells) (PubMed:14609959).2 Publications
Gene expression databases
Bgeei | ENSMUSG00000039055. |
Genevisiblei | Q8BJW7. MM. |
Interactioni
Subunit structurei
May self-associate (By similarity). Interacts with MUS81.By similarity1 Publication
Protein-protein interaction databases
BioGridi | 234502. 2 interactors. |
STRINGi | 10090.ENSMUSP00000036361. |
Structurei
3D structure databases
ProteinModelPortali | Q8BJW7. |
SMRi | Q8BJW7. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the EME1/MMS4 family.Curated
Phylogenomic databases
eggNOGi | ENOG410IE76. Eukaryota. ENOG41110FW. LUCA. |
GeneTreei | ENSGT00530000063937. |
HOGENOMi | HOG000112362. |
HOVERGENi | HBG081476. |
InParanoidi | Q8BJW7. |
KOi | K10882. |
OMAi | EAQAVPC. |
OrthoDBi | EOG091G0CET. |
PhylomeDBi | Q8BJW7. |
TreeFami | TF325310. |
Family and domain databases
InterProi | View protein in InterPro IPR006166. ERCC4_domain. IPR033310. Mms4/EME1/EME2. |
PANTHERi | PTHR21077. PTHR21077. 1 hit. |
Pfami | View protein in Pfam PF02732. ERCC4. 1 hit. |
SMARTi | View protein in SMART SM00891. ERCC4. 1 hit. |
i Sequence
Sequence statusi: Complete.
Q8BJW7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MALRRLSLSR LSTESDSEDL PTFAFLKKEP SSTNRKPPQR AKNIVVVTSD
60 70 80 90 100
SEASCPPSPG LKGPPCVPSA AGAPPQAGPV RVLSSSSEDE DVFVPLAERI
110 120 130 140 150
TCKLLTSKQL CPELSSSSLK TGLDGQNNAS APCDWKRQPW PKIPDVPLHG
160 170 180 190 200
ALEKSAANDE DSLLDDQCRQ LPTYQATCRE LAVSKTNSDR PLPKKRTKHI
210 220 230 240 250
QTVQSGGSQG CWRPGQASRK ENTPRQHERK KKAEMIKRLK AQRPEECLKH
260 270 280 290 300
IVVVLDPVLL QMEGGGQLLG ALQAMECSCV IEVQAIPRSI TWRRRRTELV
310 320 330 340 350
EDGDDWMEEP TILVLVLAEV FMSMAYNLKQ ASPSSTEKGK ETLRSFVTDV
360 370 380 390 400
TAKTGKALSL VIVDQEKCFR PQNPPRRRKS GMANKQAKAK HQQRQESSTG
410 420 430 440 450
LMVSRADMEK ALVDLQLYTE AQAWMVQSWK ELADFTCAFT KAVAEAPFKK
460 470 480 490 500
LRDQVTFSFF LEKDWAGGMK VDQSGRGLAL IWRRQIQQLN RVSSEMASAI
510 520 530 540 550
VDAYPSPQLL VQAYQRCFSE QERQNLLADI QVRRGEGVTA TSRRVGPELS
560 570
RRIYLQMTTA QPDLILDSVD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK078516 mRNA. Translation: BAC37318.1. AL645764 Genomic DNA. No translation available. BC089459 mRNA. Translation: AAH89459.1. |
CCDSi | CCDS25262.1. |
RefSeqi | NP_808420.1. NM_177752.4. |
UniGenei | Mm.358937. |
Genome annotation databases
Ensembli | ENSMUST00000039949; ENSMUSP00000036361; ENSMUSG00000039055. |
GeneIDi | 268465. |
KEGGi | mmu:268465. |
UCSCi | uc007kzf.1. mouse. |
Similar proteinsi
Entry informationi
Entry namei | EME1_MOUSE | |
Accessioni | Q8BJW7Primary (citable) accession number: Q8BJW7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 21, 2006 |
Last sequence update: | March 1, 2003 | |
Last modified: | January 31, 2018 | |
This is version 105 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |