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Protein

Crossover junction endonuclease EME1

Gene

Eme1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.1 Publication

Cofactori

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-5693568. Resolution of D-loop Structures through Holliday Junction Intermediates.
R-MMU-6783310. Fanconi Anemia Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endonuclease EME1 (EC:3.1.22.-)
Gene namesi
Name:Eme1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:3576783. Eme1.

Subcellular locationi

  • Nucleusnucleolus

  • Note: Recruited to regions of DNA damage in S-phase cells.By similarity

GO - Cellular componenti

  • cytoplasm Source: MGI
  • Holliday junction resolvase complex Source: GO_Central
  • nuclear chromatin Source: MGI
  • nuclear heterochromatin Source: MGI
  • nucleolus Source: UniProtKB-SubCell
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002236311 – 570Crossover junction endonuclease EME1Add BLAST570

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei12PhosphoserineBy similarity1
Modified residuei15PhosphoserineBy similarity1
Modified residuei84PhosphoserineBy similarity1
Modified residuei85PhosphoserineBy similarity1
Modified residuei87PhosphoserineBy similarity1
Modified residuei117PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8BJW7.
MaxQBiQ8BJW7.
PaxDbiQ8BJW7.
PeptideAtlasiQ8BJW7.
PRIDEiQ8BJW7.

PTM databases

iPTMnetiQ8BJW7.
PhosphoSitePlusiQ8BJW7.

Expressioni

Tissue specificityi

Weakly expressed in brain, heart, kidney, liver, lung, muscle, skin, small intestine, spleen, stomach, testis and thymus (PubMed:14609959, PubMed:27010503). Expressed in bone marrow (PubMed:27010503). Also expressed in embryonic stem cells (ES cells) (PubMed:14609959).2 Publications

Gene expression databases

BgeeiENSMUSG00000039055.
GenevisibleiQ8BJW7. MM.

Interactioni

Subunit structurei

May self-associate (By similarity). Interacts with MUS81.By similarity1 Publication

Protein-protein interaction databases

BioGridi234502. 2 interactors.
STRINGi10090.ENSMUSP00000036361.

Structurei

3D structure databases

ProteinModelPortaliQ8BJW7.
SMRiQ8BJW7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the EME1/MMS4 family.Curated

Phylogenomic databases

eggNOGiENOG410IE76. Eukaryota.
ENOG41110FW. LUCA.
GeneTreeiENSGT00530000063937.
HOGENOMiHOG000112362.
HOVERGENiHBG081476.
InParanoidiQ8BJW7.
KOiK10882.
OMAiSCDWKKQ.
OrthoDBiEOG091G0CET.
PhylomeDBiQ8BJW7.
TreeFamiTF325310.

Family and domain databases

InterProiIPR006166. ERCC4_domain.
IPR033310. Mms4/EME1/EME2.
[Graphical view]
PANTHERiPTHR21077. PTHR21077. 1 hit.
PfamiPF02732. ERCC4. 1 hit.
[Graphical view]
SMARTiSM00891. ERCC4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BJW7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALRRLSLSR LSTESDSEDL PTFAFLKKEP SSTNRKPPQR AKNIVVVTSD
60 70 80 90 100
SEASCPPSPG LKGPPCVPSA AGAPPQAGPV RVLSSSSEDE DVFVPLAERI
110 120 130 140 150
TCKLLTSKQL CPELSSSSLK TGLDGQNNAS APCDWKRQPW PKIPDVPLHG
160 170 180 190 200
ALEKSAANDE DSLLDDQCRQ LPTYQATCRE LAVSKTNSDR PLPKKRTKHI
210 220 230 240 250
QTVQSGGSQG CWRPGQASRK ENTPRQHERK KKAEMIKRLK AQRPEECLKH
260 270 280 290 300
IVVVLDPVLL QMEGGGQLLG ALQAMECSCV IEVQAIPRSI TWRRRRTELV
310 320 330 340 350
EDGDDWMEEP TILVLVLAEV FMSMAYNLKQ ASPSSTEKGK ETLRSFVTDV
360 370 380 390 400
TAKTGKALSL VIVDQEKCFR PQNPPRRRKS GMANKQAKAK HQQRQESSTG
410 420 430 440 450
LMVSRADMEK ALVDLQLYTE AQAWMVQSWK ELADFTCAFT KAVAEAPFKK
460 470 480 490 500
LRDQVTFSFF LEKDWAGGMK VDQSGRGLAL IWRRQIQQLN RVSSEMASAI
510 520 530 540 550
VDAYPSPQLL VQAYQRCFSE QERQNLLADI QVRRGEGVTA TSRRVGPELS
560 570
RRIYLQMTTA QPDLILDSVD
Length:570
Mass (Da):63,481
Last modified:March 1, 2003 - v1
Checksum:iF1FC9B68AD1A76A0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK078516 mRNA. Translation: BAC37318.1.
AL645764 Genomic DNA. Translation: CAI24965.1.
BC089459 mRNA. Translation: AAH89459.1.
CCDSiCCDS25262.1.
RefSeqiNP_808420.1. NM_177752.4.
UniGeneiMm.358937.

Genome annotation databases

EnsembliENSMUST00000039949; ENSMUSP00000036361; ENSMUSG00000039055.
GeneIDi268465.
KEGGimmu:268465.
UCSCiuc007kzf.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK078516 mRNA. Translation: BAC37318.1.
AL645764 Genomic DNA. Translation: CAI24965.1.
BC089459 mRNA. Translation: AAH89459.1.
CCDSiCCDS25262.1.
RefSeqiNP_808420.1. NM_177752.4.
UniGeneiMm.358937.

3D structure databases

ProteinModelPortaliQ8BJW7.
SMRiQ8BJW7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi234502. 2 interactors.
STRINGi10090.ENSMUSP00000036361.

PTM databases

iPTMnetiQ8BJW7.
PhosphoSitePlusiQ8BJW7.

Proteomic databases

EPDiQ8BJW7.
MaxQBiQ8BJW7.
PaxDbiQ8BJW7.
PeptideAtlasiQ8BJW7.
PRIDEiQ8BJW7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000039949; ENSMUSP00000036361; ENSMUSG00000039055.
GeneIDi268465.
KEGGimmu:268465.
UCSCiuc007kzf.1. mouse.

Organism-specific databases

CTDi146956.
MGIiMGI:3576783. Eme1.

Phylogenomic databases

eggNOGiENOG410IE76. Eukaryota.
ENOG41110FW. LUCA.
GeneTreeiENSGT00530000063937.
HOGENOMiHOG000112362.
HOVERGENiHBG081476.
InParanoidiQ8BJW7.
KOiK10882.
OMAiSCDWKKQ.
OrthoDBiEOG091G0CET.
PhylomeDBiQ8BJW7.
TreeFamiTF325310.

Enzyme and pathway databases

ReactomeiR-MMU-5693568. Resolution of D-loop Structures through Holliday Junction Intermediates.
R-MMU-6783310. Fanconi Anemia Pathway.

Miscellaneous databases

PROiQ8BJW7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000039055.
GenevisibleiQ8BJW7. MM.

Family and domain databases

InterProiIPR006166. ERCC4_domain.
IPR033310. Mms4/EME1/EME2.
[Graphical view]
PANTHERiPTHR21077. PTHR21077. 1 hit.
PfamiPF02732. ERCC4. 1 hit.
[Graphical view]
SMARTiSM00891. ERCC4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEME1_MOUSE
AccessioniPrimary (citable) accession number: Q8BJW7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.