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Protein

Eukaryotic translation initiation factor 2A

Gene

Eif2a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • positive regulation of signal transduction Source: MGI
  • protein phosphorylation Source: MGI
  • regulation of translation Source: UniProtKB
  • response to amino acid starvation Source: Ensembl
  • ribosome assembly Source: UniProtKB
  • SREBP signaling pathway Source: MGI
  • translation Source: MGI

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis, Translation regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 2A
Short name:
eIF-2A
Cleaved into the following chain:
Gene namesi
Name:Eif2a
Synonyms:D3Ertd194e
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1098684. Eif2a.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004244671 – 581Eukaryotic translation initiation factor 2AAdd BLAST581
Initiator methionineiRemoved; alternateBy similarity
ChainiPRO_00002860772 – 581Eukaryotic translation initiation factor 2A, N-terminally processedAdd BLAST580

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei2N-acetylalanine; in Eukaryotic translation initiation factor 2A, N-terminally processedBy similarity1
Modified residuei5PhosphothreonineBy similarity1
Modified residuei503PhosphoserineBy similarity1
Modified residuei513PhosphoserineBy similarity1
Modified residuei522PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8BJW6.
PaxDbiQ8BJW6.
PeptideAtlasiQ8BJW6.
PRIDEiQ8BJW6.

PTM databases

iPTMnetiQ8BJW6.
PhosphoSitePlusiQ8BJW6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000027810.
CleanExiMM_EIF2A.
ExpressionAtlasiQ8BJW6. baseline and differential.
GenevisibleiQ8BJW6. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi230831. 5 interactors.
IntActiQ8BJW6. 3 interactors.
MINTiMINT-1858166.
STRINGi10090.ENSMUSP00000029387.

Structurei

3D structure databases

ProteinModelPortaliQ8BJW6.
SMRiQ8BJW6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati23 – 63WD 1Add BLAST41
Repeati125 – 163WD 2Add BLAST39
Repeati356 – 401WD 3Add BLAST46

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili527 – 578Sequence analysisAdd BLAST52

Sequence similaritiesi

Belongs to the WD repeat EIF2A family.Curated

Keywords - Domaini

Coiled coil, Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG2315. Eukaryota.
COG5354. LUCA.
GeneTreeiENSGT00730000111053.
HOGENOMiHOG000193775.
HOVERGENiHBG107842.
InParanoidiQ8BJW6.
KOiK15026.
OMAiWQPYTTS.
OrthoDBiEOG091G04PE.
PhylomeDBiQ8BJW6.
TreeFamiTF105866.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiView protein in InterPro
IPR011387. TIF2A.
IPR013979. TIF_beta_prop-like.
IPR015943. WD40/YVTN_repeat-like_dom_sf.
IPR036322. WD40_repeat_dom_sf.
PfamiView protein in Pfam
PF08662. eIF2A. 1 hit.
PIRSFiPIRSF017222. eIF2A. 1 hit.
SUPFAMiSSF50978. SSF50978. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BJW6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPSTPLLTV RGSEGLYMVN GPPHFTESTV LPRESGRNCK VYTFSKDGTL
60 70 80 90 100
FAWSNGEKVN IINVANKGLL HSFDLPKAVC LEFSPNNTVL ATWQPYTTSK
110 120 130 140 150
DGTAGTPNLQ LYDMKTGACL KSFIQKKMQN WCPSWSDDEI ICARNVNNEV
160 170 180 190 200
HFFENNNFNT IANKLHLQKV NDFNLSPGTQ PYKVAVYVPG SKGAPSFVRL
210 220 230 240 250
YQYPNFAGPQ AALANKSFFK ADKVTMLWNK KATAVLVIAS TEVDKTGASY
260 270 280 290 300
YGEQTLHYIA TNGESAVVQL PKNGPIYDVV WNSSSTEFCA VYGFMPAKAT
310 320 330 340 350
VFNLKCDPVF DFGTGPRNAA FYSPHGHILV LAGFGNLRGQ MEVWDVKNYK
360 370 380 390 400
LISKPVASDS TYFAWCPDGE HILTATCAPR LRVNNGYKIW HYTGSLLHKY
410 420 430 440 450
DVPSNGELWQ VSWQPFLDGI FPAKTIKYQA VPSEVPSEEP KVATAYRPPA
460 470 480 490 500
LRNKPVTNSK LHEEEPPQNM KPHPGSDKPL SKTALKNQRK HEAKKAAKQE
510 520 530 540 550
ARSDAAPTPV PQSAPRNTVT QSASGDPEVD KKIKNLKKKL KAIEQLKEQA
560 570 580
AAGKQLEKNQ LEKIQKETAL LQELEDLELG V
Length:581
Mass (Da):64,403
Last modified:May 1, 2007 - v2
Checksum:iD485C05ACE5D0908
GO
Isoform 2 (identifier: Q8BJW6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-94: Missing.
     95-97: PYT → MEK

Note: No experimental confirmation available.
Show »
Length:487
Mass (Da):54,136
Checksum:i369EDD5BDBCAC95E
GO

Sequence cautioni

The sequence BAC37320 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0249771 – 94Missing in isoform 2. 1 PublicationAdd BLAST94
Alternative sequenceiVSP_02497895 – 97PYT → MEK in isoform 2. 1 Publication3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK078519 mRNA. Translation: BAC37320.1. Different initiation.
BC030447 mRNA. Translation: AAH30447.1.
BC082557 mRNA. Translation: AAH82557.1.
CCDSiCCDS17367.1. [Q8BJW6-1]
RefSeqiNP_001005509.1. NM_001005509.2. [Q8BJW6-1]
UniGeneiMm.21617.

Genome annotation databases

EnsembliENSMUST00000029387; ENSMUSP00000029387; ENSMUSG00000027810. [Q8BJW6-1]
GeneIDi229317.
KEGGimmu:229317.
UCSCiuc008phu.2. mouse. [Q8BJW6-1]
uc008phv.2. mouse. [Q8BJW6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiEIF2A_MOUSE
AccessioniPrimary (citable) accession number: Q8BJW6
Secondary accession number(s): Q05C11, Q640P8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: May 1, 2007
Last modified: November 22, 2017
This is version 115 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families