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Protein

Eukaryotic translation initiation factor 2A

Gene

Eif2a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • positive regulation of signal transduction Source: MGI
  • protein phosphorylation Source: MGI
  • regulation of translation Source: UniProtKB
  • ribosome assembly Source: UniProtKB
  • SREBP signaling pathway Source: MGI
  • translation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis, Translation regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 2A
Short name:
eIF-2A
Cleaved into the following chain:
Gene namesi
Name:Eif2a
Synonyms:D3Ertd194e
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1098684. Eif2a.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 581581Eukaryotic translation initiation factor 2APRO_0000286077Add
BLAST
Initiator methionineiRemoved; alternateBy similarity
Chaini2 – 581580Eukaryotic translation initiation factor 2A, N-terminally processedPRO_0000424467Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei2 – 21N-acetylalanine; in Eukaryotic translation initiation factor 2A, N-terminally processedBy similarity
Modified residuei503 – 5031PhosphoserineBy similarity
Modified residuei513 – 5131PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8BJW6.
MaxQBiQ8BJW6.
PaxDbiQ8BJW6.
PRIDEiQ8BJW6.

PTM databases

iPTMnetiQ8BJW6.
PhosphoSiteiQ8BJW6.

Expressioni

Gene expression databases

BgeeiQ8BJW6.
CleanExiMM_EIF2A.
ExpressionAtlasiQ8BJW6. baseline and differential.
GenevisibleiQ8BJW6. MM.

Interactioni

Protein-protein interaction databases

BioGridi230831. 5 interactions.
IntActiQ8BJW6. 3 interactions.
MINTiMINT-1858166.
STRINGi10090.ENSMUSP00000029387.

Structurei

3D structure databases

ProteinModelPortaliQ8BJW6.
SMRiQ8BJW6. Positions 11-415.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati23 – 6341WD 1Add
BLAST
Repeati125 – 16339WD 2Add
BLAST
Repeati356 – 40146WD 3Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili527 – 57852Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the WD repeat EIF2A family.Curated
Contains 3 WD repeats.Curated

Keywords - Domaini

Coiled coil, Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG2315. Eukaryota.
COG5354. LUCA.
GeneTreeiENSGT00730000111053.
HOGENOMiHOG000193775.
HOVERGENiHBG107842.
InParanoidiQ8BJW6.
KOiK15026.
OMAiPIYDVAW.
OrthoDBiEOG7GBFX2.
PhylomeDBiQ8BJW6.
TreeFamiTF105866.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR011387. TIF2A.
IPR013979. TIF_beta_prop-like.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08662. eIF2A. 1 hit.
[Graphical view]
PIRSFiPIRSF017222. eIF2A. 1 hit.
SUPFAMiSSF50978. SSF50978. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BJW6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPSTPLLTV RGSEGLYMVN GPPHFTESTV LPRESGRNCK VYTFSKDGTL
60 70 80 90 100
FAWSNGEKVN IINVANKGLL HSFDLPKAVC LEFSPNNTVL ATWQPYTTSK
110 120 130 140 150
DGTAGTPNLQ LYDMKTGACL KSFIQKKMQN WCPSWSDDEI ICARNVNNEV
160 170 180 190 200
HFFENNNFNT IANKLHLQKV NDFNLSPGTQ PYKVAVYVPG SKGAPSFVRL
210 220 230 240 250
YQYPNFAGPQ AALANKSFFK ADKVTMLWNK KATAVLVIAS TEVDKTGASY
260 270 280 290 300
YGEQTLHYIA TNGESAVVQL PKNGPIYDVV WNSSSTEFCA VYGFMPAKAT
310 320 330 340 350
VFNLKCDPVF DFGTGPRNAA FYSPHGHILV LAGFGNLRGQ MEVWDVKNYK
360 370 380 390 400
LISKPVASDS TYFAWCPDGE HILTATCAPR LRVNNGYKIW HYTGSLLHKY
410 420 430 440 450
DVPSNGELWQ VSWQPFLDGI FPAKTIKYQA VPSEVPSEEP KVATAYRPPA
460 470 480 490 500
LRNKPVTNSK LHEEEPPQNM KPHPGSDKPL SKTALKNQRK HEAKKAAKQE
510 520 530 540 550
ARSDAAPTPV PQSAPRNTVT QSASGDPEVD KKIKNLKKKL KAIEQLKEQA
560 570 580
AAGKQLEKNQ LEKIQKETAL LQELEDLELG V
Length:581
Mass (Da):64,403
Last modified:May 1, 2007 - v2
Checksum:iD485C05ACE5D0908
GO
Isoform 2 (identifier: Q8BJW6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-94: Missing.
     95-97: PYT → MEK

Note: No experimental confirmation available.
Show »
Length:487
Mass (Da):54,136
Checksum:i369EDD5BDBCAC95E
GO

Sequence cautioni

The sequence BAC37320.1 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 9494Missing in isoform 2. 1 PublicationVSP_024977Add
BLAST
Alternative sequencei95 – 973PYT → MEK in isoform 2. 1 PublicationVSP_024978

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK078519 mRNA. Translation: BAC37320.1. Different initiation.
BC030447 mRNA. Translation: AAH30447.1.
BC082557 mRNA. Translation: AAH82557.1.
CCDSiCCDS17367.1. [Q8BJW6-1]
RefSeqiNP_001005509.1. NM_001005509.2. [Q8BJW6-1]
UniGeneiMm.21617.

Genome annotation databases

EnsembliENSMUST00000029387; ENSMUSP00000029387; ENSMUSG00000027810. [Q8BJW6-1]
GeneIDi229317.
KEGGimmu:229317.
UCSCiuc008phu.2. mouse. [Q8BJW6-1]
uc008phv.2. mouse. [Q8BJW6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK078519 mRNA. Translation: BAC37320.1. Different initiation.
BC030447 mRNA. Translation: AAH30447.1.
BC082557 mRNA. Translation: AAH82557.1.
CCDSiCCDS17367.1. [Q8BJW6-1]
RefSeqiNP_001005509.1. NM_001005509.2. [Q8BJW6-1]
UniGeneiMm.21617.

3D structure databases

ProteinModelPortaliQ8BJW6.
SMRiQ8BJW6. Positions 11-415.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230831. 5 interactions.
IntActiQ8BJW6. 3 interactions.
MINTiMINT-1858166.
STRINGi10090.ENSMUSP00000029387.

PTM databases

iPTMnetiQ8BJW6.
PhosphoSiteiQ8BJW6.

Proteomic databases

EPDiQ8BJW6.
MaxQBiQ8BJW6.
PaxDbiQ8BJW6.
PRIDEiQ8BJW6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029387; ENSMUSP00000029387; ENSMUSG00000027810. [Q8BJW6-1]
GeneIDi229317.
KEGGimmu:229317.
UCSCiuc008phu.2. mouse. [Q8BJW6-1]
uc008phv.2. mouse. [Q8BJW6-2]

Organism-specific databases

CTDi83939.
MGIiMGI:1098684. Eif2a.

Phylogenomic databases

eggNOGiKOG2315. Eukaryota.
COG5354. LUCA.
GeneTreeiENSGT00730000111053.
HOGENOMiHOG000193775.
HOVERGENiHBG107842.
InParanoidiQ8BJW6.
KOiK15026.
OMAiPIYDVAW.
OrthoDBiEOG7GBFX2.
PhylomeDBiQ8BJW6.
TreeFamiTF105866.

Miscellaneous databases

ChiTaRSiEif2a. mouse.
PROiQ8BJW6.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BJW6.
CleanExiMM_EIF2A.
ExpressionAtlasiQ8BJW6. baseline and differential.
GenevisibleiQ8BJW6. MM.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR011387. TIF2A.
IPR013979. TIF_beta_prop-like.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08662. eIF2A. 1 hit.
[Graphical view]
PIRSFiPIRSF017222. eIF2A. 1 hit.
SUPFAMiSSF50978. SSF50978. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Muellerian duct.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Eye.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiEIF2A_MOUSE
AccessioniPrimary (citable) accession number: Q8BJW6
Secondary accession number(s): Q05C11, Q640P8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: May 1, 2007
Last modified: June 8, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.