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Protein

Mdm2-binding protein

Gene

Mtbp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in MDM2-dependent p53/TP53 homeostasis in unstressed cells. Inhibits autoubiquitination of MDM2, thereby enhancing MDM2 stability. This promotes MDM2-mediated ubiquitination of p53/TP53 and its subsequent degradation. Inhibits cell migration in vitro and suppresses the invasive behavior of tumor cells.2 Publications

GO - Biological processi

  • cell cycle arrest Source: UniProtKB-KW
  • negative regulation of cell proliferation Source: MGI
  • negative regulation of mitotic nuclear division Source: MGI
  • protein localization to kinetochore Source: MGI
  • traversing start control point of mitotic cell cycle Source: MGI

Keywordsi

Biological processCell cycle, Growth arrest, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Mdm2-binding protein
Short name:
mMTBP
Gene namesi
Name:Mtbp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:2146005. Mtbp.

Subcellular locationi

Pathology & Biotechi

Disruption phenotypei

Death between E3.5 and E7.5. Mice containing a heterozygous deletion of this gene coupled with heterozygous deletion of TP53 exhibit an increased incidence of metastatic tumors.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003237461 – 894Mdm2-binding proteinAdd BLAST894

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei591PhosphoserineBy similarity1
Modified residuei633PhosphoserineCombined sources1
Modified residuei697PhosphoserineBy similarity1
Modified residuei701PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8BJS8.
PaxDbiQ8BJS8.
PeptideAtlasiQ8BJS8.
PRIDEiQ8BJS8.

PTM databases

iPTMnetiQ8BJS8.
PhosphoSitePlusiQ8BJS8.

Expressioni

Tissue specificityi

Expressed in small intestine and spleen at low levels, in the ovary at intermediate levels and in the testis and thymus at high levels.1 Publication

Gene expression databases

BgeeiENSMUSG00000022369.
ExpressionAtlasiQ8BJS8. baseline and differential.
GenevisibleiQ8BJS8. MM.

Interactioni

Subunit structurei

Interacts with MDM2.1 Publication

Protein-protein interaction databases

MINTiMINT-139747.
STRINGi10090.ENSMUSP00000022998.

Structurei

3D structure databases

ProteinModelPortaliQ8BJS8.
SMRiQ8BJS8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni515 – 894Interaction with MDM21 PublicationAdd BLAST380

Sequence similaritiesi

Belongs to the MTBP family.Curated

Phylogenomic databases

eggNOGiENOG410IIA4. Eukaryota.
ENOG410YVAG. LUCA.
GeneTreeiENSGT00390000003305.
HOGENOMiHOG000113675.
HOVERGENiHBG108152.
InParanoidiQ8BJS8.
OMAiFPEFCLK.
OrthoDBiEOG091G01OL.
PhylomeDBiQ8BJS8.
TreeFamiTF331503.

Family and domain databases

InterProiView protein in InterPro
IPR029418. MTBP_C.
IPR029420. MTBP_central.
IPR029421. MTBP_N.
PfamiView protein in Pfam
PF14920. MTBP_C. 1 hit.
PF14919. MTBP_mid. 1 hit.
PF14918. MTBP_N. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BJS8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDRYLLLVTW REGKFRSVAG GEIEPGTEAT SLESTDKQPD LTATNIYHLL
60 70 80 90 100
KRSISDSIHP DDSTFPACSV GGTPHSRKWF FAVQAICGFY QFCSSDWQEI
110 120 130 140 150
HFDAEKDKIE DVLQANIEEC QSAVECFEED DSNSRESLPL ADLYEESAEN
160 170 180 190 200
LHQLSDKLPA PGRAMIDIIL LPSDKDPPKL KECLPIVGAL KHLKEWHSAK
210 220 230 240 250
VIIAGSYCEI NCQKIAEYLS ASVVPLEEFR NAIDPRELWR GEIQMRERKF
260 270 280 290 300
GFEISFPEFC LKGVTPTNVS AYNLNTCFLA KKIASSKVFH YYGPALEFVQ
310 320 330 340 350
MIKLSDLPSC YMSDIEFELE VTGHCTRQNS MLLLEQISSL CGKVGALFVL
360 370 380 390 400
PCTVSNVLIP PPSQLASRKW KEYMAKKPKT ISVPDVAVKG EFSGYHLLLQ
410 420 430 440 450
GMGKRKCRAT LLHSASQING SFALSVIHGK MKTKAGEARP SFPFDFSSLP
460 470 480 490 500
RFSEEQVLQR EKQLASFQVL ALKECLKRRK AANQPEAFSA DELKSLLALT
510 520 530 540 550
RERFLGHFDV LPTEAALAQT DTVKAAGVVN DDGTVEPYSS SLMETNPLEW
560 570 580 590 600
PERHVLQNLE TSEKAKQKMR TGSLPRSSEQ LLGHKEGPRD SLTLLDAKEL
610 620 630 640 650
LKYFTSDGLP VGDLQPLHIQ RGEKPFVLTP ELSPGKLQVL PFEKASECHY
660 670 680 690 700
HGIEYCLDDQ KALERDGGFS ELQSRLIRYE TQTTCTRDSF PVPTVLSPLP
710 720 730 740 750
SPAVLSEPQS VPEGEALQGE LRTEVSGLKR RSKDPSCLYP QKRLTRSESS
760 770 780 790 800
DCLPSQASCN SNHHHHTGKP RKPQAERCVS GLPLPGREAS KDTSKTSSGQ
810 820 830 840 850
KRAHESKSSK QMKESRSQKH TRMLKEVVKD TLKRHHITEA HESFTACSQR
860 870 880 890
LFDISKFYLK DLKTSRGLFE EMKKTANNNV VQVIEWVVEK MSKK
Length:894
Mass (Da):100,314
Last modified:March 1, 2003 - v1
Checksum:i93528336ED081A27
GO
Isoform 2 (identifier: Q8BJS8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     623-629: EKPFVLT → IHQLVHV
     630-894: Missing.

Note: No experimental confirmation available.
Show »
Length:629
Mass (Da):70,400
Checksum:iF94D0B9DA698C1B7
GO
Isoform 3 (identifier: Q8BJS8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-373: Missing.

Note: No experimental confirmation available.
Show »
Length:521
Mass (Da):58,294
Checksum:i76E748D97E7F9C3E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti120C → R in CAC07970 (PubMed:10906133).Curated1
Sequence conflicti143L → V in CAC07970 (PubMed:10906133).Curated1
Sequence conflicti178P → R in CAC07970 (PubMed:10906133).Curated1
Sequence conflicti238L → V in CAC07970 (PubMed:10906133).Curated1
Sequence conflicti256F → L in CAC07970 (PubMed:10906133).Curated1
Sequence conflicti524K → R in AAH52197 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0321051 – 373Missing in isoform 3. 1 PublicationAdd BLAST373
Alternative sequenceiVSP_032106623 – 629EKPFVLT → IHQLVHV in isoform 2. 1 Publication7
Alternative sequenceiVSP_032107630 – 894Missing in isoform 2. 1 PublicationAdd BLAST265

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ278508 mRNA. Translation: CAC07970.1.
AK080036 mRNA. Translation: BAC37810.1.
AK088549 mRNA. Translation: BAC40418.1.
AK088652 mRNA. Translation: BAC40479.1.
AK133855 mRNA. Translation: BAE21889.1.
BC005500 mRNA. Translation: AAH05500.1.
BC052197 mRNA. Translation: AAH52197.1.
CCDSiCCDS27478.1. [Q8BJS8-1]
CCDS49609.1. [Q8BJS8-2]
RefSeqiNP_001161722.1. NM_001168250.1. [Q8BJS8-2]
NP_598853.3. NM_134092.3. [Q8BJS8-1]
UniGeneiMm.390829.

Genome annotation databases

EnsembliENSMUST00000022998; ENSMUSP00000022998; ENSMUSG00000022369. [Q8BJS8-1]
ENSMUST00000169667; ENSMUSP00000128615; ENSMUSG00000022369. [Q8BJS8-3]
ENSMUST00000170046; ENSMUSP00000129396; ENSMUSG00000022369. [Q8BJS8-2]
GeneIDi105837.
KEGGimmu:105837.
UCSCiuc007vsi.1. mouse. [Q8BJS8-1]
uc011zsz.1. mouse. [Q8BJS8-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMTBP_MOUSE
AccessioniPrimary (citable) accession number: Q8BJS8
Secondary accession number(s): Q80VU3
, Q8BTU9, Q8BTW0, Q99K25, Q9ER61
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 1, 2003
Last modified: May 10, 2017
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families