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Protein

Chondroitin sulfate N-acetylgalactosaminyltransferase 1

Gene

Csgalnact1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transfers 1,4-N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of glucuronic acid (GlcUA). Required for addition of the first GalNAc to the core tetrasaccharide linker and for elongation of chondroitin chains. Important role in chondroitin chain biosynthesis in cartilage formation, and subsequent endochondral ossification (PubMed:17145758, PubMed:21148564). Moreover, is involved in the metabolism of aggrecan (PubMed:21148564).2 Publications

Catalytic activityi

UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-(1->3)-D-galactosyl-proteoglycan = UDP + N-acetyl-D-galactosaminyl-(1->4)-beta-D-glucuronyl-(1->3)-beta-D-galactosylproteoglycan.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi358 – 3581Divalent metal cationSequence analysis
Metal bindingi475 – 4751Divalent metal cationSequence analysis

GO - Molecular functioni

GO - Biological processi

  • cartilage development Source: MGI
  • chondroitin sulfate biosynthetic process Source: UniProtKB
  • chondroitin sulfate metabolic process Source: MGI
  • chondroitin sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process Source: UniProtKB
  • endochondral ossification Source: MGI
  • extracellular matrix organization Source: MGI
  • UDP-glucuronate metabolic process Source: UniProtKB
  • UDP-N-acetylgalactosamine metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-2022870. Chondroitin sulfate biosynthesis.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

Names & Taxonomyi

Protein namesi
Recommended name:
Chondroitin sulfate N-acetylgalactosaminyltransferase 1 (EC:2.4.1.174)
Short name:
CsGalNAcT-1
Alternative name(s):
Chondroitin beta-1,4-N-acetylgalactosaminyltransferase 1
Short name:
Beta4GalNAcT-1
Gene namesi
Name:Csgalnact1
Synonyms:Chgn, Galnact1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:2442354. Csgalnact1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1212CytoplasmicSequence analysisAdd
BLAST
Transmembranei13 – 3321Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini34 – 530497LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Deficient mice are viable and fertile, but decreased body weight and length and abnormal cartilage (PubMed:21148564).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 530530Chondroitin sulfate N-acetylgalactosaminyltransferase 1PRO_0000189565Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi313 – 3131N-linked (GlcNAc...)Sequence analysis
Glycosylationi322 – 3221N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ8BJQ9.
PaxDbiQ8BJQ9.
PRIDEiQ8BJQ9.

PTM databases

iPTMnetiQ8BJQ9.
PhosphoSiteiQ8BJQ9.

Expressioni

Developmental stagei

High expression in developing cartilage and during chondrocyte differentiation.1 Publication

Gene expression databases

BgeeiENSMUSG00000036356.
GenevisibleiQ8BJQ9. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000077459.

Structurei

3D structure databases

ProteinModelPortaliQ8BJQ9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili57 – 9337Sequence analysisAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3588. Eukaryota.
ENOG410XNYM. LUCA.
GeneTreeiENSGT00760000119143.
HOVERGENiHBG050930.
InParanoidiQ8BJQ9.
KOiK00746.
OMAiGKEEMNE.
OrthoDBiEOG091G070I.
PhylomeDBiQ8BJQ9.
TreeFamiTF318303.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR008428. Chond_GalNAc.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF05679. CHGN. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 2 hits.

Sequencei

Sequence statusi: Complete.

Q8BJQ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRRGLLGWI SRVVILLVLL CCAISVLYML ACTPKGDQEQ LGLPRANGPT
60 70 80 90 100
GKDGYQAVLQ EREEQHRNYV NSLKRQIAQL KDELQARSEQ FRSGQDQASD
110 120 130 140 150
ATSLRSGWDR EPKAQADLLA FLRGQVDKAE VHAGVKLATE YAAVPFDSFT
160 170 180 190 200
LQKVYQLETG LTRHPEEKPV RKDKRDELVE AIESALESLN SPVESSPHQR
210 220 230 240 250
PYTAADFIEG IYRTERDKGT LYELTFKGDH KHEFQRLVLF RPFGPIMKVK
260 270 280 290 300
KEKLNLANTL INVIVPLARR VDKFRHFMQN FREMCIQQDG RVHLTVVYFG
310 320 330 340 350
KEEMNEVKGI LENTSKAANF RNFTFIQLNG EFSRGKGLDV GARFWKGSNV
360 370 380 390 400
LLFFCDVDIY FTSEFLNTCR LNTQPGKKVF YPVLFSQYNP GVIYGHHDAV
410 420 430 440 450
PPLGQQLVIK KETGFWRDFG FGMTCQYRSD FINIGGFDLD IKGWGGEDVH
460 470 480 490 500
LYRKYLHSNL IVVRTPVRGL FHLWHEKHCM DELTPEQYKM CMQSKAMNEA
510 520 530
SHGQLGMLVF RHEIEAHLRK QKQKASSKKT
Length:530
Mass (Da):60,917
Last modified:March 1, 2003 - v1
Checksum:i0DE695EDEB7F7765
GO

Sequence cautioni

The sequence BAE25692 differs from that shown. Reason: Erroneous termination at position 491. Translated as Cys.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti163 – 1631R → P in BAE25692 (PubMed:16141072).Curated
Sequence conflicti186 – 1861L → M in BAC26066 (PubMed:16141072).Curated
Sequence conflicti189 – 1891L → V in BAC28340 (PubMed:16141072).Curated
Sequence conflicti393 – 3931I → V in AAH89162 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028688 mRNA. Translation: BAC26066.1.
AK033522 mRNA. Translation: BAC28340.1.
AK049770 mRNA. Translation: BAC33912.1.
AK080687 mRNA. Translation: BAC37982.1.
AK133567 mRNA. Translation: BAE21729.1.
AK144089 mRNA. Translation: BAE25692.1. Sequence problems.
BC026599 mRNA. Translation: AAH26599.1.
BC089162 mRNA. Translation: AAH89162.1.
CCDSiCCDS22341.1.
RefSeqiNP_001239552.1. NM_001252623.1.
NP_766341.4. NM_172753.5.
XP_006509687.1. XM_006509624.2.
XP_006509688.1. XM_006509625.2.
XP_006509689.1. XM_006509626.1.
XP_006509690.1. XM_006509627.2.
XP_006509691.1. XM_006509628.2.
XP_006509692.1. XM_006509629.1.
XP_006509693.1. XM_006509630.2.
XP_011240592.1. XM_011242290.1.
UniGeneiMm.334569.
Mm.489712.

Genome annotation databases

EnsembliENSMUST00000078350; ENSMUSP00000077459; ENSMUSG00000036356.
ENSMUST00000130214; ENSMUSP00000119817; ENSMUSG00000036356.
GeneIDi234356.
KEGGimmu:234356.
UCSCiuc009lwf.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028688 mRNA. Translation: BAC26066.1.
AK033522 mRNA. Translation: BAC28340.1.
AK049770 mRNA. Translation: BAC33912.1.
AK080687 mRNA. Translation: BAC37982.1.
AK133567 mRNA. Translation: BAE21729.1.
AK144089 mRNA. Translation: BAE25692.1. Sequence problems.
BC026599 mRNA. Translation: AAH26599.1.
BC089162 mRNA. Translation: AAH89162.1.
CCDSiCCDS22341.1.
RefSeqiNP_001239552.1. NM_001252623.1.
NP_766341.4. NM_172753.5.
XP_006509687.1. XM_006509624.2.
XP_006509688.1. XM_006509625.2.
XP_006509689.1. XM_006509626.1.
XP_006509690.1. XM_006509627.2.
XP_006509691.1. XM_006509628.2.
XP_006509692.1. XM_006509629.1.
XP_006509693.1. XM_006509630.2.
XP_011240592.1. XM_011242290.1.
UniGeneiMm.334569.
Mm.489712.

3D structure databases

ProteinModelPortaliQ8BJQ9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000077459.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

PTM databases

iPTMnetiQ8BJQ9.
PhosphoSiteiQ8BJQ9.

Proteomic databases

MaxQBiQ8BJQ9.
PaxDbiQ8BJQ9.
PRIDEiQ8BJQ9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000078350; ENSMUSP00000077459; ENSMUSG00000036356.
ENSMUST00000130214; ENSMUSP00000119817; ENSMUSG00000036356.
GeneIDi234356.
KEGGimmu:234356.
UCSCiuc009lwf.3. mouse.

Organism-specific databases

CTDi55790.
MGIiMGI:2442354. Csgalnact1.

Phylogenomic databases

eggNOGiKOG3588. Eukaryota.
ENOG410XNYM. LUCA.
GeneTreeiENSGT00760000119143.
HOVERGENiHBG050930.
InParanoidiQ8BJQ9.
KOiK00746.
OMAiGKEEMNE.
OrthoDBiEOG091G070I.
PhylomeDBiQ8BJQ9.
TreeFamiTF318303.

Enzyme and pathway databases

ReactomeiR-MMU-2022870. Chondroitin sulfate biosynthesis.

Miscellaneous databases

PROiQ8BJQ9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000036356.
GenevisibleiQ8BJQ9. MM.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR008428. Chond_GalNAc.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF05679. CHGN. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiCGAT1_MOUSE
AccessioniPrimary (citable) accession number: Q8BJQ9
Secondary accession number(s): Q3UNQ5
, Q3UZX6, Q5FWX8, Q8BWV9, Q8BZU7, Q8C195, Q8R0M6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: March 1, 2003
Last modified: September 7, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.