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Q8BJQ2

- UBP1_MOUSE

UniProt

Q8BJQ2 - UBP1_MOUSE

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Protein
Ubiquitin carboxyl-terminal hydrolase 1
Gene
Usp1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei90 – 901Nucleophile By similarityBy similarity
Active sitei592 – 5921Proton acceptor By similarityBy similarity
Sitei669 – 6702Cleavage; by autolysis By similarityBy similarity

GO - Molecular functioni

  1. ubiquitin thiolesterase activity Source: UniProtKB
  2. ubiquitin-specific protease activity Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. DNA repair Source: UniProtKB-KW
  2. monoubiquitinated protein deubiquitination Source: UniProtKB
  3. protein deubiquitination Source: UniProtKB
  4. regulation of DNA repair Source: UniProtKB
  5. response to UV Source: UniProtKB
  6. skeletal system development Source: MGI
  7. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

DNA damage, DNA repair, Ubl conjugation pathway

Protein family/group databases

MEROPSiC19.019.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 1 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 1
Ubiquitin thioesterase 1
Ubiquitin-specific-processing protease 1
Gene namesi
Name:Usp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:2385198. Usp1.

Subcellular locationi

Nucleus By similarity By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 784784Ubiquitin carboxyl-terminal hydrolase 1
PRO_0000306287Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei42 – 421Phosphoserine By similarityBy similarity
Modified residuei67 – 671Phosphoserine By similarity
Modified residuei474 – 4741Phosphoserine By similarity

Post-translational modificationi

Autocatalytic cleavage of USP1 following UV irradiation inactivates it leading to an increase in ubiquitinated PCNA, recruitment of POLH and translesion synthesis By similarity.
Ubiquitinated; leading to its subsequent proteasomal degradation By similarity.

Keywords - PTMi

Autocatalytic cleavage, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ8BJQ2.
PRIDEiQ8BJQ2.

PTM databases

PhosphoSiteiQ8BJQ2.

Expressioni

Gene expression databases

ArrayExpressiQ8BJQ2.
BgeeiQ8BJQ2.
CleanExiMM_USP1.
GenevestigatoriQ8BJQ2.

Interactioni

Subunit structurei

Interacts with FANCD2 and PCNA. Interacts with WDR48 By similarity.

Protein-protein interaction databases

BioGridi230966. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ8BJQ2.
SMRiQ8BJQ2. Positions 378-650.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini81 – 784704USP
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.
Contains 1 USP domain.

Phylogenomic databases

eggNOGiCOG5077.
GeneTreeiENSGT00650000093027.
HOGENOMiHOG000154756.
HOVERGENiHBG017288.
InParanoidiQ8BJQ2.
KOiK11832.
OMAiFAVVMHS.
OrthoDBiEOG7JT6VZ.
PhylomeDBiQ8BJQ2.
TreeFamiTF331057.

Family and domain databases

InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamiPF00443. UCH. 2 hits.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BJQ2-1 [UniParc]FASTAAdd to Basket

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MPGVIPSESN GLSRGSPSKK NRLSLKFFQK KETKRALDFT DSQENEEKTS    50
EYRGSEIDQV VPAAQSSPVS CEKRENLLPF VGLNNLGNTC YLNSILQVLY 100
FCPGFKTGVK HLFNIISRKK EALKDDSNQK DKGSCKEESL ASYELICSLQ 150
SLIISVEQLQ ASFLLNPEKY TDELATQPRR LLNTLRELNP MYEGFLQHDA 200
QEVLQCILGN IQETCQLLKK EEIKNLAELS GKVEEQSLQK EETGGITSTE 250
IDSMRNTEDV KEQLPKGNWK RKSDSESSNV KKKVKLSRES QPLEENQRQT 300
RSKRKAIGDT LEAAPKIIPK CVSESESAKP SQKKSKVKIN WLKPSTKQPS 350
ILSKFCSLGK ITTNQRSKGQ PKEKEGDVEE DLEKYGSDHT ANGGPESPGS 400
SVTPVDSSEA KSGNKGAEQI GFELVEKLFQ GQLVLRTRCL ECESLTERRE 450
DFQDISVPVQ EDELSKVEES SEISPEPKTE MKTLRWAISQ FASVERIVGE 500
DKYFCENCHH YTEAERSLLF DKMPEVITIH LKCFAASGLE FDCYGGGLSK 550
INTPLLTPLK LSLEEWSTKP TNDSYGLFAV VMHSGITISS GHYTASVKVT 600
DLNSLELDEG NFVVDQMCEL GKPEPLTEEQ ARGTAGNYDD EVSIRVGGNA 650
QPSKVLNKKN VEGIGLLGGQ KSKADYELYN KASNPDKVVG TPFTDNRNSE 700
TNDTTNGTHE SDRNKESSDQ TGVNMNGLEN KISYVVQSLK EYEGKWLLFD 750
DSEVKVTEEK DFLNSLSPST SPTSTPYLLF YKKL 784
Length:784
Mass (Da):87,456
Last modified:March 1, 2003 - v1
Checksum:i56F849DC1ADE3D81
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti84 – 841N → K in AAH20007. 1 Publication
Sequence conflicti344 – 3441P → S in AAH18179. 1 Publication
Sequence conflicti344 – 3441P → S in AAH20007. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK080882 mRNA. Translation: BAC38059.1.
AL627349 Genomic DNA. Translation: CAM21066.1.
BC018179 mRNA. Translation: AAH18179.1.
BC020007 mRNA. Translation: AAH20007.1.
CCDSiCCDS18381.1.
RefSeqiNP_666256.2. NM_146144.3.
XP_006503001.1. XM_006502938.1.
UniGeneiMm.371692.

Genome annotation databases

EnsembliENSMUST00000030289; ENSMUSP00000030289; ENSMUSG00000028560.
ENSMUST00000091358; ENSMUSP00000088917; ENSMUSG00000028560.
GeneIDi230484.
KEGGimmu:230484.
UCSCiuc008tum.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK080882 mRNA. Translation: BAC38059.1 .
AL627349 Genomic DNA. Translation: CAM21066.1 .
BC018179 mRNA. Translation: AAH18179.1 .
BC020007 mRNA. Translation: AAH20007.1 .
CCDSi CCDS18381.1.
RefSeqi NP_666256.2. NM_146144.3.
XP_006503001.1. XM_006502938.1.
UniGenei Mm.371692.

3D structure databases

ProteinModelPortali Q8BJQ2.
SMRi Q8BJQ2. Positions 378-650.
ModBasei Search...

Protein-protein interaction databases

BioGridi 230966. 1 interaction.

Protein family/group databases

MEROPSi C19.019.

PTM databases

PhosphoSitei Q8BJQ2.

Proteomic databases

PaxDbi Q8BJQ2.
PRIDEi Q8BJQ2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000030289 ; ENSMUSP00000030289 ; ENSMUSG00000028560 .
ENSMUST00000091358 ; ENSMUSP00000088917 ; ENSMUSG00000028560 .
GeneIDi 230484.
KEGGi mmu:230484.
UCSCi uc008tum.1. mouse.

Organism-specific databases

CTDi 7398.
MGIi MGI:2385198. Usp1.

Phylogenomic databases

eggNOGi COG5077.
GeneTreei ENSGT00650000093027.
HOGENOMi HOG000154756.
HOVERGENi HBG017288.
InParanoidi Q8BJQ2.
KOi K11832.
OMAi FAVVMHS.
OrthoDBi EOG7JT6VZ.
PhylomeDBi Q8BJQ2.
TreeFami TF331057.

Miscellaneous databases

NextBioi 379913.
PROi Q8BJQ2.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q8BJQ2.
Bgeei Q8BJQ2.
CleanExi MM_USP1.
Genevestigatori Q8BJQ2.

Family and domain databases

InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view ]
Pfami PF00443. UCH. 2 hits.
[Graphical view ]
PROSITEi PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Adipose tissue.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.

Entry informationi

Entry nameiUBP1_MOUSE
AccessioniPrimary (citable) accession number: Q8BJQ2
Secondary accession number(s): Q8VE17, Q8VEM4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: March 1, 2003
Last modified: September 3, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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