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Protein

Ubiquitin carboxyl-terminal hydrolase 1

Gene

Usp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity.By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei90NucleophilePROSITE-ProRule annotation1
Active sitei592Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

DNA damage, DNA repair, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-MMU-6783310. Fanconi Anemia Pathway.

Protein family/group databases

MEROPSiC19.019.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 1 (EC:3.4.19.12By similarity)
Alternative name(s):
Deubiquitinating enzyme 1
Ubiquitin thioesterase 1
Ubiquitin-specific-processing protease 1
Gene namesi
Name:Usp1Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2385198. Usp1.

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003062871 – 784Ubiquitin carboxyl-terminal hydrolase 1Add BLAST784

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei16PhosphoserineBy similarity1
Modified residuei42PhosphoserineBy similarity1
Modified residuei67PhosphoserineBy similarity1
Modified residuei474PhosphoserineBy similarity1
Modified residuei767PhosphoserineCombined sources1

Post-translational modificationi

Autocatalytic cleavage of USP1 following UV irradiation inactivates it leading to an increase in ubiquitinated PCNA, recruitment of POLH and translesion synthesis.By similarity
Ubiquitinated; leading to its subsequent proteasomal degradation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei669 – 670Cleavage; by autolysisBy similarity2

Keywords - PTMi

Autocatalytic cleavage, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8BJQ2.
MaxQBiQ8BJQ2.
PaxDbiQ8BJQ2.
PeptideAtlasiQ8BJQ2.
PRIDEiQ8BJQ2.

PTM databases

iPTMnetiQ8BJQ2.
PhosphoSitePlusiQ8BJQ2.

Expressioni

Gene expression databases

BgeeiENSMUSG00000028560.
CleanExiMM_USP1.
ExpressionAtlasiQ8BJQ2. baseline and differential.
GenevisibleiQ8BJQ2. MM.

Interactioni

Subunit structurei

Interacts with FANCD2 and PCNA. Interacts with WDR48.By similarity

Protein-protein interaction databases

BioGridi230966. 1 interactor.
STRINGi10090.ENSMUSP00000030289.

Structurei

3D structure databases

ProteinModelPortaliQ8BJQ2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini81 – 784USPAdd BLAST704

Sequence similaritiesi

Belongs to the peptidase C19 family.Sequence analysis
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiKOG1864. Eukaryota.
ENOG410XQ81. LUCA.
GeneTreeiENSGT00850000132276.
HOGENOMiHOG000154756.
HOVERGENiHBG017288.
InParanoidiQ8BJQ2.
KOiK11832.
OMAiEYRASEI.
OrthoDBiEOG091G04D9.
PhylomeDBiQ8BJQ2.
TreeFamiTF331057.

Family and domain databases

CDDicd02671. Peptidase_C19O. 1 hit.
InterProiIPR001394. Peptidase_C19_UCH.
IPR033815. USP1.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BJQ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGVIPSESN GLSRGSPSKK NRLSLKFFQK KETKRALDFT DSQENEEKTS
60 70 80 90 100
EYRGSEIDQV VPAAQSSPVS CEKRENLLPF VGLNNLGNTC YLNSILQVLY
110 120 130 140 150
FCPGFKTGVK HLFNIISRKK EALKDDSNQK DKGSCKEESL ASYELICSLQ
160 170 180 190 200
SLIISVEQLQ ASFLLNPEKY TDELATQPRR LLNTLRELNP MYEGFLQHDA
210 220 230 240 250
QEVLQCILGN IQETCQLLKK EEIKNLAELS GKVEEQSLQK EETGGITSTE
260 270 280 290 300
IDSMRNTEDV KEQLPKGNWK RKSDSESSNV KKKVKLSRES QPLEENQRQT
310 320 330 340 350
RSKRKAIGDT LEAAPKIIPK CVSESESAKP SQKKSKVKIN WLKPSTKQPS
360 370 380 390 400
ILSKFCSLGK ITTNQRSKGQ PKEKEGDVEE DLEKYGSDHT ANGGPESPGS
410 420 430 440 450
SVTPVDSSEA KSGNKGAEQI GFELVEKLFQ GQLVLRTRCL ECESLTERRE
460 470 480 490 500
DFQDISVPVQ EDELSKVEES SEISPEPKTE MKTLRWAISQ FASVERIVGE
510 520 530 540 550
DKYFCENCHH YTEAERSLLF DKMPEVITIH LKCFAASGLE FDCYGGGLSK
560 570 580 590 600
INTPLLTPLK LSLEEWSTKP TNDSYGLFAV VMHSGITISS GHYTASVKVT
610 620 630 640 650
DLNSLELDEG NFVVDQMCEL GKPEPLTEEQ ARGTAGNYDD EVSIRVGGNA
660 670 680 690 700
QPSKVLNKKN VEGIGLLGGQ KSKADYELYN KASNPDKVVG TPFTDNRNSE
710 720 730 740 750
TNDTTNGTHE SDRNKESSDQ TGVNMNGLEN KISYVVQSLK EYEGKWLLFD
760 770 780
DSEVKVTEEK DFLNSLSPST SPTSTPYLLF YKKL
Length:784
Mass (Da):87,456
Last modified:March 1, 2003 - v1
Checksum:i56F849DC1ADE3D81
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti84N → K in AAH20007 (PubMed:15489334).Curated1
Sequence conflicti344P → S in AAH18179 (PubMed:15489334).Curated1
Sequence conflicti344P → S in AAH20007 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK080882 mRNA. Translation: BAC38059.1.
AL627349 Genomic DNA. Translation: CAM21066.1.
BC018179 mRNA. Translation: AAH18179.1.
BC020007 mRNA. Translation: AAH20007.1.
CCDSiCCDS18381.1.
RefSeqiNP_001288343.1. NM_001301414.1.
NP_666256.2. NM_146144.4.
UniGeneiMm.371692.

Genome annotation databases

EnsembliENSMUST00000030289; ENSMUSP00000030289; ENSMUSG00000028560.
ENSMUST00000091358; ENSMUSP00000088917; ENSMUSG00000028560.
GeneIDi230484.
KEGGimmu:230484.
UCSCiuc008tum.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK080882 mRNA. Translation: BAC38059.1.
AL627349 Genomic DNA. Translation: CAM21066.1.
BC018179 mRNA. Translation: AAH18179.1.
BC020007 mRNA. Translation: AAH20007.1.
CCDSiCCDS18381.1.
RefSeqiNP_001288343.1. NM_001301414.1.
NP_666256.2. NM_146144.4.
UniGeneiMm.371692.

3D structure databases

ProteinModelPortaliQ8BJQ2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230966. 1 interactor.
STRINGi10090.ENSMUSP00000030289.

Protein family/group databases

MEROPSiC19.019.

PTM databases

iPTMnetiQ8BJQ2.
PhosphoSitePlusiQ8BJQ2.

Proteomic databases

EPDiQ8BJQ2.
MaxQBiQ8BJQ2.
PaxDbiQ8BJQ2.
PeptideAtlasiQ8BJQ2.
PRIDEiQ8BJQ2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030289; ENSMUSP00000030289; ENSMUSG00000028560.
ENSMUST00000091358; ENSMUSP00000088917; ENSMUSG00000028560.
GeneIDi230484.
KEGGimmu:230484.
UCSCiuc008tum.2. mouse.

Organism-specific databases

CTDi7398.
MGIiMGI:2385198. Usp1.

Phylogenomic databases

eggNOGiKOG1864. Eukaryota.
ENOG410XQ81. LUCA.
GeneTreeiENSGT00850000132276.
HOGENOMiHOG000154756.
HOVERGENiHBG017288.
InParanoidiQ8BJQ2.
KOiK11832.
OMAiEYRASEI.
OrthoDBiEOG091G04D9.
PhylomeDBiQ8BJQ2.
TreeFamiTF331057.

Enzyme and pathway databases

ReactomeiR-MMU-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-MMU-6783310. Fanconi Anemia Pathway.

Miscellaneous databases

PROiQ8BJQ2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028560.
CleanExiMM_USP1.
ExpressionAtlasiQ8BJQ2. baseline and differential.
GenevisibleiQ8BJQ2. MM.

Family and domain databases

CDDicd02671. Peptidase_C19O. 1 hit.
InterProiIPR001394. Peptidase_C19_UCH.
IPR033815. USP1.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBP1_MOUSE
AccessioniPrimary (citable) accession number: Q8BJQ2
Secondary accession number(s): Q8VE17, Q8VEM4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: March 1, 2003
Last modified: November 30, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.