Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

F-box only protein 30

Gene

Fbxo30

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Required for muscle atrophy following denervation.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri49 – 110TRAF-typePROSITE-ProRule annotationAdd BLAST62

GO - Molecular functioni

Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
F-box only protein 30
Alternative name(s):
Muscle ubiquitin ligase of SCF complex in atrophy-1
Short name:
MUSA1
Gene namesi
Name:Fbxo30
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1919115. Fbxo30.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001199191 – 746F-box only protein 30Add BLAST746

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei383PhosphoserineBy similarity1

Post-translational modificationi

Auto-ubiquitinated.1 Publication
May be neddylated. Neddylation may be required for E3 ligase activity, since it was observed only after purification with o-phenanthroline (PubMed:24076600).1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8BJL1.
MaxQBiQ8BJL1.
PaxDbiQ8BJL1.
PeptideAtlasiQ8BJL1.
PRIDEiQ8BJL1.

PTM databases

iPTMnetiQ8BJL1.
PhosphoSitePlusiQ8BJL1.

Expressioni

Inductioni

Up-regulated in denervated muscles (at protein level), with highest expression levels around 14 days following denervation. Negatively regulated by the bone morphogenetic protein (BMP) pathway.1 Publication

Gene expression databases

BgeeiENSMUSG00000047648.
CleanExiMM_FBXO30.
GenevisibleiQ8BJL1. MM.

Interactioni

Subunit structurei

Part of a SCF (SKP1-cullin-F-box) protein ligase complex. Interacts with SKP1, CUL1 and RBX1/ROC1.1 Publication

Protein-protein interaction databases

BioGridi214990. 1 interactor.
MINTiMINT-1340690.
STRINGi10090.ENSMUSP00000068230.

Structurei

3D structure databases

ProteinModelPortaliQ8BJL1.
SMRiQ8BJL1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini611 – 659F-boxPROSITE-ProRule annotationAdd BLAST49

Sequence similaritiesi

Contains 1 F-box domain.PROSITE-ProRule annotation
Contains 1 TRAF-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri49 – 110TRAF-typePROSITE-ProRule annotationAdd BLAST62

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410IH36. Eukaryota.
ENOG410XQPH. LUCA.
GeneTreeiENSGT00410000025582.
HOGENOMiHOG000059618.
HOVERGENiHBG051574.
InParanoidiQ8BJL1.
KOiK10307.
OMAiRNKVAEH.
OrthoDBiEOG091G06SE.
PhylomeDBiQ8BJL1.
TreeFamiTF343227.

Family and domain databases

Gene3Di3.90.890.10. 1 hit.
InterProiIPR001810. F-box_dom.
IPR031890. Fbxo30/Fbxo40.
IPR013323. SIAH-type.
IPR008974. TRAF-like.
IPR001293. Znf_TRAF.
[Graphical view]
PANTHERiPTHR15933. PTHR15933. 3 hits.
PfamiPF15966. F-box_4. 1 hit.
PF15965. zf-TRAF_2. 1 hit.
[Graphical view]
SUPFAMiSSF49599. SSF49599. 1 hit.
SSF81383. SSF81383. 1 hit.
PROSITEiPS50181. FBOX. 1 hit.
PS50145. ZF_TRAF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BJL1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEEVQQHSH CMNCVSRRCM TRPEPGVSCD LIGCPLVCGA VFHSCKADEH
60 70 80 90 100
RLLCPFERVA CLNRNFGCPF TLARNKVAEH LEMCPASVVC CTMEWNRWPV
110 120 130 140 150
SYSDRKSYES LSRDVDEVAQ LDMALALQDQ RMLLESLKVA TMMSKATDKI
160 170 180 190 200
SEPREQISVK SSVQEIPRTN GLVSVDEESY GALYQATVET TRSLAAALDI
210 220 230 240 250
LNSATRDIGM LNTSLHATAN EMDEENNKES FQDKNLKDQD HLDEGEIGAV
260 270 280 290 300
GGVDYSGTSQ NAQAEQNGSS DLLCDLNPSS NGTSALCNGF PLEKMCIQVK
310 320 330 340 350
GQDQNFHGDS TESNITNGDC VEADGTSEPS SSLVVPEQLR EISPFSALPD
360 370 380 390 400
STFQQILMPD EDDEKDLCWK KVDLGDLKDV NGSPFSHAPS FKFLSNSWYI
410 420 430 440 450
PKEDKAVDTS DLEVAEDPMG LQGIDLITAA LLFCLGDSPG GRGISDSRMT
460 470 480 490 500
DVYHVDFGTQ TFSLPSAILA TNTMVGEIAS ASACDHANPQ LSNPSPFQTL
510 520 530 540 550
GLDLVLECVA RYQPKQRSMF TFVCGQLFRR KEFSSHFKNV HGDIHAGLNG
560 570 580 590 600
WMEQRCPLAY YGCTYSQRRF CPSTQGAKII HDRHLRSFGV QPCVSTVLEE
610 620 630 640 650
PSRNCVLGLR SDHLSSLPFE VLQHIAGFLD GFSLCQLACV SRLMRDVCGS
660 670 680 690 700
LLQSRGMVIL QWGKKKYPEG NSSWQIKEKV WRFSTAFCSV NDWKFADILS
710 720 730 740
MADHLKNCSY NVIEKREEAI PLPCMCVTRE LTKEGRSLRS VLKPVL
Length:746
Mass (Da):82,687
Last modified:July 27, 2011 - v2
Checksum:iFD88404AFC9560D0
GO

Sequence cautioni

The sequence AAH37691 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti87S → G in BAB24551 (PubMed:16141072).Curated1
Sequence conflicti140A → P in BAC38931 (PubMed:16141072).Curated1
Sequence conflicti296C → G in BAB24551 (PubMed:16141072).Curated1
Sequence conflicti431L → S in BAB24551 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK006369 mRNA. Translation: BAB24551.1.
AK083479 mRNA. Translation: BAC38931.1.
AK135274 mRNA. Translation: BAE22469.1.
CH466562 Genomic DNA. Translation: EDL03517.1.
BC037691 mRNA. Translation: AAH37691.1. Different initiation.
CCDSiCCDS23697.1.
RefSeqiNP_001161769.1. NM_001168297.1.
NP_082244.2. NM_027968.3.
XP_006512922.1. XM_006512859.3.
UniGeneiMm.276229.

Genome annotation databases

EnsembliENSMUST00000070300; ENSMUSP00000068230; ENSMUSG00000047648.
ENSMUST00000129456; ENSMUSP00000117687; ENSMUSG00000047648.
GeneIDi71865.
KEGGimmu:71865.
UCSCiuc007ejt.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK006369 mRNA. Translation: BAB24551.1.
AK083479 mRNA. Translation: BAC38931.1.
AK135274 mRNA. Translation: BAE22469.1.
CH466562 Genomic DNA. Translation: EDL03517.1.
BC037691 mRNA. Translation: AAH37691.1. Different initiation.
CCDSiCCDS23697.1.
RefSeqiNP_001161769.1. NM_001168297.1.
NP_082244.2. NM_027968.3.
XP_006512922.1. XM_006512859.3.
UniGeneiMm.276229.

3D structure databases

ProteinModelPortaliQ8BJL1.
SMRiQ8BJL1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi214990. 1 interactor.
MINTiMINT-1340690.
STRINGi10090.ENSMUSP00000068230.

PTM databases

iPTMnetiQ8BJL1.
PhosphoSitePlusiQ8BJL1.

Proteomic databases

EPDiQ8BJL1.
MaxQBiQ8BJL1.
PaxDbiQ8BJL1.
PeptideAtlasiQ8BJL1.
PRIDEiQ8BJL1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000070300; ENSMUSP00000068230; ENSMUSG00000047648.
ENSMUST00000129456; ENSMUSP00000117687; ENSMUSG00000047648.
GeneIDi71865.
KEGGimmu:71865.
UCSCiuc007ejt.2. mouse.

Organism-specific databases

CTDi84085.
MGIiMGI:1919115. Fbxo30.

Phylogenomic databases

eggNOGiENOG410IH36. Eukaryota.
ENOG410XQPH. LUCA.
GeneTreeiENSGT00410000025582.
HOGENOMiHOG000059618.
HOVERGENiHBG051574.
InParanoidiQ8BJL1.
KOiK10307.
OMAiRNKVAEH.
OrthoDBiEOG091G06SE.
PhylomeDBiQ8BJL1.
TreeFamiTF343227.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

ChiTaRSiFbxo30. mouse.
PROiQ8BJL1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000047648.
CleanExiMM_FBXO30.
GenevisibleiQ8BJL1. MM.

Family and domain databases

Gene3Di3.90.890.10. 1 hit.
InterProiIPR001810. F-box_dom.
IPR031890. Fbxo30/Fbxo40.
IPR013323. SIAH-type.
IPR008974. TRAF-like.
IPR001293. Znf_TRAF.
[Graphical view]
PANTHERiPTHR15933. PTHR15933. 3 hits.
PfamiPF15966. F-box_4. 1 hit.
PF15965. zf-TRAF_2. 1 hit.
[Graphical view]
SUPFAMiSSF49599. SSF49599. 1 hit.
SSF81383. SSF81383. 1 hit.
PROSITEiPS50181. FBOX. 1 hit.
PS50145. ZF_TRAF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFBX30_MOUSE
AccessioniPrimary (citable) accession number: Q8BJL1
Secondary accession number(s): Q3UXU4, Q8CI21, Q9D9X5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.