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Q8BJL0 (SMAL1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1

EC=3.6.4.-
Alternative name(s):
HepA-related protein
Short name=mharp
Sucrose nonfermenting protein 2-like 1
Gene names
Name:Smarcal1
Synonyms:Harp
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length910 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

ATP-dependent annealing helicase that catalyzes the rewinding of the stably unwound DNA. Rewinds single-stranded DNA bubbles that are stably bound by replication protein A (RPA). Acts throughout the genome to reanneal stably unwound DNA, performing the opposite reaction of many enzymes, such as helicases and polymerases, that unwind DNA By similarity.

Subcellular location

Nucleus By similarity.

Tissue specificity

Ubiquitously expressed, with high levels in brain, heart, kidney, liver and testis. Ref.1

Sequence similarities

Belongs to the SNF2/RAD54 helicase family. SMARCAL1 subfamily.

Contains 2 HARP domains.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8BJL0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8BJL0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     769-788: LIQAEDRVHRIGQTNSVSIH → GNVARVPLGMPRAEKYKIRK
     789-910: Missing.
Note: May be due to an intron retention.
Isoform 3 (identifier: Q8BJL0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     508-574: DESHFLKNIK...HAFGLRYCDA → VSNGIALKYF...QPLSLCARLS
     575-910: Missing.
Note: No experimental confirmation available.
Isoform 4 (identifier: Q8BJL0-4)

The sequence of this isoform differs from the canonical sequence as follows:
     404-404: S → R
     405-910: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 910909SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1
PRO_0000074349

Regions

Domain199 – 26971HARP 1
Domain286 – 35772HARP 2
Domain404 – 559156Helicase ATP-binding
Domain674 – 827154Helicase C-terminal
Nucleotide binding417 – 4248ATP By similarity
Coiled coil3 – 3432 Potential
Motif508 – 5114DESH box

Amino acid modifications

Modified residue21N-acetylserine By similarity

Natural variations

Alternative sequence4041S → R in isoform 4.
VSP_036216
Alternative sequence405 – 910506Missing in isoform 4.
VSP_036217
Alternative sequence508 – 57467DESHF…RYCDA → VSNGIALKYFVCLDTRKGST DLGICVLLGPLWALRREGNR NRCLSFIENDFFIPFLKQPL SLCARLS in isoform 3.
VSP_036218
Alternative sequence575 – 910336Missing in isoform 3.
VSP_036219
Alternative sequence769 – 78820LIQAE…SVSIH → GNVARVPLGMPRAEKYKIRK in isoform 2.
VSP_012919
Alternative sequence789 – 910122Missing in isoform 2.
VSP_012920

Experimental info

Sequence conflict351A → T in AAH29078. Ref.3
Sequence conflict781F → L in AAH29078. Ref.3
Sequence conflict5291K → N in BAE32320. Ref.2
Sequence conflict6851K → R in BAC31469. Ref.2
Sequence conflict717 – 7259Missing in AAG47648. Ref.1
Sequence conflict7171T → R in AAF24985. Ref.1
Sequence conflict719 – 7213SAD → TRA in AAF24985. Ref.1
Sequence conflict724 – 7252AQ → LK in AAF24985. Ref.1
Sequence conflict7431T → P in AAF24985. Ref.1
Sequence conflict8341D → A in AAF24985. Ref.1
Sequence conflict8341Missing in AAG47648. Ref.1
Sequence conflict866 – 8683LGS → IKN in AAF24985. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: F6B28F847BD6FC18

FASTA910100,840
        10         20         30         40         50         60 
MSLPLTEEQR KKIEENRQKA LARRAEKLSE QPQSAASGSS AAGPSQSKQG SLLNLLAEPS 

        70         80         90        100        110        120 
KPVGHASIFK QQNLSNSFPT DQRPHSSRCS QPSPAEETTG LWKTQGEMST ACPKPNPSPP 

       130        140        150        160        170        180 
GASNQPLLGY KSSEGQPQAT WDTGASSSGP FPRDPELEAK AARPSTSRQS ISDSFYVLGG 

       190        200        210        220        230        240 
KTPRTEGRPP NILQTTPQNT GFLRGACIKT GDRFRVKIGY NQELIAVFKS LPSRHYDSFT 

       250        260        270        280        290        300 
KTWDFSMSDY RALMKAVERL STVSLKPLDE AGGSVGGQTS LPSAPSLTFV TGKCMLISRV 

       310        320        330        340        350        360 
RFEVDIGYSE AVIGLFKQME SRSYDIKTRK WSFLLEEHNK LIARSRELKQ VQLDPLPKTV 

       370        380        390        400        410        420 
TLAFASQLEK TSPKLKADVP EADLSGVDAK LVSSLMPFQR EGVSFAISKR GRLLLADDMG 

       430        440        450        460        470        480 
LGKTVQAICI AAFYRKEWPL LVVVPSSVRF TWEQAFLRWL PSLSPENINV VVTGKGRLTA 

       490        500        510        520        530        540 
GLVNIVSFDL LCKLERQLKT PFKVVIIDES HFLKNIKTAR CRAAVPILKV AKRVILLSGT 

       550        560        570        580        590        600 
PAMSRPAELY TQIIAVKPTF FPQFHAFGLR YCDAKRLPWG WDYSGSSNLG ELKLLLEEAI 

       610        620        630        640        650        660 
MLRRLKSDVL SQLPAKQRKM VVVNPGRISS RAKAALDAAA KEMTKDKTKQ QQKEALLVFF 

       670        680        690        700        710        720 
NRTAEAKIPC VVEYILDLLD SGREKFLVFA HHKVILDAVA KELERKNVQH IRIDGSTPSA 

       730        740        750        760        770        780 
DREAQCQRFQ LSKGHTVALL SITAANMGLT FSTADLVVFA ELFWNPGVLI QAEDRVHRIG 

       790        800        810        820        830        840 
QTNSVSIHYL VAKGTADDYL WPLIQEKIKV LGEAGLSETN FSEMTEATDY VHKDPKQKTI 

       850        860        870        880        890        900 
YDLFQQSFED DGNDMEFLEA AESFELGSTS GTSGNISQDL GDLLDEDEGS PPKKSRFEFF 

       910 
DNWDSFSSPF 

« Hide

Isoform 2 [UniParc].

Checksum: AE70BAB0A799A3F8
Show »

FASTA78887,126
Isoform 3 [UniParc].

Checksum: 0E8930A229E115E8
Show »

FASTA57463,218
Isoform 4 [UniParc].

Checksum: 92E5249A242D75A6
Show »

FASTA40444,190

References

« Hide 'large scale' references
[1]"Cloning and characterization of HARP/SMARCAL1: a prokaryotic HepA-related SNF2 helicase protein from human and mouse."
Coleman M.A., Eisen J.A., Mohrenweiser H.W.
Genomics 65:274-282(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2; 3 AND 4).
Strain: C57BL/6J and NOD.
Tissue: Cerebellum, Embryo, Testis and Thymus.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: Czech II.
Tissue: Mammary gland.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF088884 mRNA. Translation: AAF24985.1.
AF209773 Genomic DNA. Translation: AAG47648.1.
AK042332 mRNA. Translation: BAE20630.1.
AK043129 mRNA. Translation: BAC31469.1.
AK077878 mRNA. Translation: BAC37047.1.
AK083488 mRNA. Translation: BAC38933.1.
AK154020 mRNA. Translation: BAE32320.1.
AK169465 mRNA. Translation: BAE41189.1.
BC029078 mRNA. Translation: AAH29078.1.
RefSeqNP_061287.2. NM_018817.2.
XP_006496204.1. XM_006496141.1.
XP_006496205.1. XM_006496142.1.
XP_006496206.1. XM_006496143.1.
XP_006496207.1. XM_006496144.1.
UniGeneMm.274232.

3D structure databases

ProteinModelPortalQ8BJL0.
SMRQ8BJL0. Positions 393-807.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000047589.

PTM databases

PhosphoSiteQ8BJL0.

Proteomic databases

PaxDbQ8BJL0.
PRIDEQ8BJL0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000047615; ENSMUSP00000047589; ENSMUSG00000039354. [Q8BJL0-1]
ENSMUST00000152225; ENSMUSP00000137833; ENSMUSG00000039354. [Q8BJL0-1]
GeneID54380.
KEGGmmu:54380.
UCSCuc007bko.2. mouse. [Q8BJL0-4]
uc007bkq.2. mouse. [Q8BJL0-3]
uc007bkr.2. mouse. [Q8BJL0-2]
uc007bks.2. mouse. [Q8BJL0-1]

Organism-specific databases

CTD50485.
MGIMGI:1859183. Smarcal1.

Phylogenomic databases

eggNOGCOG0553.
GeneTreeENSGT00630000089754.
HOVERGENHBG054110.
InParanoidQ3TEQ9.
KOK14440.
OMAFKQQNLS.
OrthoDBEOG7XSTD5.
PhylomeDBQ8BJL0.
TreeFamTF106474.

Gene expression databases

BgeeQ8BJL0.
GenevestigatorQ8BJL0.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR010003. HARP_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view]
PfamPF07443. HARP. 2 hits.
PF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 3 hits.
PROSITEPS51467. HARP. 2 hits.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio311200.
PROQ8BJL0.
SOURCESearch...

Entry information

Entry nameSMAL1_MOUSE
AccessionPrimary (citable) accession number: Q8BJL0
Secondary accession number(s): Q3TEQ9 expand/collapse secondary AC list , Q3U4W0, Q3V3A8, Q8BVK7, Q8BXW4, Q8K309, Q9EQK8, Q9QYC4
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2003
Last modified: April 16, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot