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Q8BJL0

- SMAL1_MOUSE

UniProt

Q8BJL0 - SMAL1_MOUSE

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Protein

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1

Gene
Smarcal1, Harp
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

ATP-dependent annealing helicase that binds selectively to fork DNA relative to ssDNA or dsDNA and catalyzes the rewinding of the stably unwound DNA. Rewinds single-stranded DNA bubbles that are stably bound by replication protein A (RPA). Acts throughout the genome to reanneal stably unwound DNA, performing the opposite reaction of many enzymes, such as helicases and polymerases, that unwind DNA. May play an important role in DNA damage response by acting at stalled replication forks By similarity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi417 – 4248ATP By similarity

GO - Molecular functioni

  1. annealing helicase activity Source: UniProtKB
  2. ATP binding Source: UniProtKB-KW
  3. DNA binding Source: InterPro
  4. DNA-dependent ATPase activity Source: UniProtKB
  5. helicase activity Source: UniProtKB-KW

GO - Biological processi

  1. ATP catabolic process Source: GOC
  2. cellular response to DNA damage stimulus Source: UniProtKB
  3. chromatin modification Source: InterPro
  4. DNA metabolic process Source: UniProtKB
  5. regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  6. replication fork processing Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1 (EC:3.6.4.-)
Alternative name(s):
HepA-related protein
Short name:
mharp
Sucrose nonfermenting protein 2-like 1
Gene namesi
Name:Smarcal1
Synonyms:Harp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:1859183. Smarcal1.

Subcellular locationi

Nucleus By similarity
Note: Recruited to damaged DNA regions By similarity.

GO - Cellular componenti

  1. nucleus Source: UniProtKB
  2. site of double-strand break Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 910909SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1PRO_0000074349Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine By similarity

Post-translational modificationi

DNA damage-regulated phosphorylation by kinases that may include ATM, ATR and PRKDC By similarity.

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ8BJL0.
PRIDEiQ8BJL0.

PTM databases

PhosphoSiteiQ8BJL0.

Expressioni

Tissue specificityi

Ubiquitously expressed, with high levels in brain, heart, kidney, liver and testis.1 Publication

Gene expression databases

BgeeiQ8BJL0.
GenevestigatoriQ8BJL0.

Interactioni

Subunit structurei

Interacts with RPA2; the interaction is direct and mediates the recruitment by the RPA complex of SMARCAL1 to sites of DNA damage By similarity.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000047589.

Structurei

Secondary structure

1
910
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi203 – 2108
Beta strandi213 – 2186
Helixi222 – 2309
Beta strandi235 – 2373
Turni238 – 2414
Beta strandi242 – 2465
Helixi247 – 2493
Helixi250 – 2578
Beta strandi263 – 2664

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4O66X-ray1.90A/B/C/D197-268[»]
ProteinModelPortaliQ8BJL0.
SMRiQ8BJL0. Positions 393-807.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini199 – 26971HARP 1Add
BLAST
Domaini286 – 35772HARP 2Add
BLAST
Domaini404 – 559156Helicase ATP-bindingAdd
BLAST
Domaini674 – 827154Helicase C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 2928Mediates interaction with RPA2 By similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili3 – 3432 Reviewed predictionAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi508 – 5114DESH box

Sequence similaritiesi

Contains 2 HARP domains.

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiCOG0553.
GeneTreeiENSGT00630000089754.
HOVERGENiHBG054110.
InParanoidiQ3TEQ9.
KOiK14440.
OMAiSIHYLVA.
OrthoDBiEOG7XSTD5.
PhylomeDBiQ8BJL0.
TreeFamiTF106474.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR010003. HARP_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view]
PfamiPF07443. HARP. 2 hits.
PF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS51467. HARP. 2 hits.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8BJL0-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSLPLTEEQR KKIEENRQKA LARRAEKLSE QPQSAASGSS AAGPSQSKQG    50
SLLNLLAEPS KPVGHASIFK QQNLSNSFPT DQRPHSSRCS QPSPAEETTG 100
LWKTQGEMST ACPKPNPSPP GASNQPLLGY KSSEGQPQAT WDTGASSSGP 150
FPRDPELEAK AARPSTSRQS ISDSFYVLGG KTPRTEGRPP NILQTTPQNT 200
GFLRGACIKT GDRFRVKIGY NQELIAVFKS LPSRHYDSFT KTWDFSMSDY 250
RALMKAVERL STVSLKPLDE AGGSVGGQTS LPSAPSLTFV TGKCMLISRV 300
RFEVDIGYSE AVIGLFKQME SRSYDIKTRK WSFLLEEHNK LIARSRELKQ 350
VQLDPLPKTV TLAFASQLEK TSPKLKADVP EADLSGVDAK LVSSLMPFQR 400
EGVSFAISKR GRLLLADDMG LGKTVQAICI AAFYRKEWPL LVVVPSSVRF 450
TWEQAFLRWL PSLSPENINV VVTGKGRLTA GLVNIVSFDL LCKLERQLKT 500
PFKVVIIDES HFLKNIKTAR CRAAVPILKV AKRVILLSGT PAMSRPAELY 550
TQIIAVKPTF FPQFHAFGLR YCDAKRLPWG WDYSGSSNLG ELKLLLEEAI 600
MLRRLKSDVL SQLPAKQRKM VVVNPGRISS RAKAALDAAA KEMTKDKTKQ 650
QQKEALLVFF NRTAEAKIPC VVEYILDLLD SGREKFLVFA HHKVILDAVA 700
KELERKNVQH IRIDGSTPSA DREAQCQRFQ LSKGHTVALL SITAANMGLT 750
FSTADLVVFA ELFWNPGVLI QAEDRVHRIG QTNSVSIHYL VAKGTADDYL 800
WPLIQEKIKV LGEAGLSETN FSEMTEATDY VHKDPKQKTI YDLFQQSFED 850
DGNDMEFLEA AESFELGSTS GTSGNISQDL GDLLDEDEGS PPKKSRFEFF 900
DNWDSFSSPF 910
Length:910
Mass (Da):100,840
Last modified:March 1, 2003 - v1
Checksum:iF6B28F847BD6FC18
GO
Isoform 2 (identifier: Q8BJL0-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     769-788: LIQAEDRVHRIGQTNSVSIH → GNVARVPLGMPRAEKYKIRK
     789-910: Missing.

Note: May be due to an intron retention.

Show »
Length:788
Mass (Da):87,126
Checksum:iAE70BAB0A799A3F8
GO
Isoform 3 (identifier: Q8BJL0-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     508-574: DESHFLKNIK...HAFGLRYCDA → VSNGIALKYF...QPLSLCARLS
     575-910: Missing.

Note: No experimental confirmation available.

Show »
Length:574
Mass (Da):63,218
Checksum:i0E8930A229E115E8
GO
Isoform 4 (identifier: Q8BJL0-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     404-404: S → R
     405-910: Missing.

Note: No experimental confirmation available.

Show »
Length:404
Mass (Da):44,190
Checksum:i92E5249A242D75A6
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei404 – 4041S → R in isoform 4. VSP_036216
Alternative sequencei405 – 910506Missing in isoform 4. VSP_036217Add
BLAST
Alternative sequencei508 – 57467DESHF…RYCDA → VSNGIALKYFVCLDTRKGST DLGICVLLGPLWALRREGNR NRCLSFIENDFFIPFLKQPL SLCARLS in isoform 3. VSP_036218Add
BLAST
Alternative sequencei575 – 910336Missing in isoform 3. VSP_036219Add
BLAST
Alternative sequencei769 – 78820LIQAE…SVSIH → GNVARVPLGMPRAEKYKIRK in isoform 2. VSP_012919Add
BLAST
Alternative sequencei789 – 910122Missing in isoform 2. VSP_012920Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti35 – 351A → T in AAH29078. 1 Publication
Sequence conflicti78 – 781F → L in AAH29078. 1 Publication
Sequence conflicti529 – 5291K → N in BAE32320. 1 Publication
Sequence conflicti685 – 6851K → R in BAC31469. 1 Publication
Sequence conflicti717 – 7259Missing in AAG47648. 1 Publication
Sequence conflicti717 – 7171T → R in AAF24985. 1 Publication
Sequence conflicti719 – 7213SAD → TRA in AAF24985. 1 Publication
Sequence conflicti724 – 7252AQ → LK in AAF24985. 1 Publication
Sequence conflicti743 – 7431T → P in AAF24985. 1 Publication
Sequence conflicti834 – 8341D → A in AAF24985. 1 Publication
Sequence conflicti834 – 8341Missing in AAG47648. 1 Publication
Sequence conflicti866 – 8683LGS → IKN in AAF24985. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF088884 mRNA. Translation: AAF24985.1.
AF209773 Genomic DNA. Translation: AAG47648.1.
AK042332 mRNA. Translation: BAE20630.1.
AK043129 mRNA. Translation: BAC31469.1.
AK077878 mRNA. Translation: BAC37047.1.
AK083488 mRNA. Translation: BAC38933.1.
AK154020 mRNA. Translation: BAE32320.1.
AK169465 mRNA. Translation: BAE41189.1.
BC029078 mRNA. Translation: AAH29078.1.
CCDSiCCDS35609.1. [Q8BJL0-1]
RefSeqiNP_061287.2. NM_018817.2. [Q8BJL0-1]
XP_006496204.1. XM_006496141.1. [Q8BJL0-1]
XP_006496205.1. XM_006496142.1. [Q8BJL0-1]
XP_006496206.1. XM_006496143.1. [Q8BJL0-1]
XP_006496207.1. XM_006496144.1. [Q8BJL0-1]
UniGeneiMm.274232.

Genome annotation databases

EnsembliENSMUST00000047615; ENSMUSP00000047589; ENSMUSG00000039354. [Q8BJL0-1]
ENSMUST00000152225; ENSMUSP00000137833; ENSMUSG00000039354. [Q8BJL0-1]
GeneIDi54380.
KEGGimmu:54380.
UCSCiuc007bko.2. mouse. [Q8BJL0-4]
uc007bkq.2. mouse. [Q8BJL0-3]
uc007bkr.2. mouse. [Q8BJL0-2]
uc007bks.2. mouse. [Q8BJL0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF088884 mRNA. Translation: AAF24985.1 .
AF209773 Genomic DNA. Translation: AAG47648.1 .
AK042332 mRNA. Translation: BAE20630.1 .
AK043129 mRNA. Translation: BAC31469.1 .
AK077878 mRNA. Translation: BAC37047.1 .
AK083488 mRNA. Translation: BAC38933.1 .
AK154020 mRNA. Translation: BAE32320.1 .
AK169465 mRNA. Translation: BAE41189.1 .
BC029078 mRNA. Translation: AAH29078.1 .
CCDSi CCDS35609.1. [Q8BJL0-1 ]
RefSeqi NP_061287.2. NM_018817.2. [Q8BJL0-1 ]
XP_006496204.1. XM_006496141.1. [Q8BJL0-1 ]
XP_006496205.1. XM_006496142.1. [Q8BJL0-1 ]
XP_006496206.1. XM_006496143.1. [Q8BJL0-1 ]
XP_006496207.1. XM_006496144.1. [Q8BJL0-1 ]
UniGenei Mm.274232.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4O66 X-ray 1.90 A/B/C/D 197-268 [» ]
ProteinModelPortali Q8BJL0.
SMRi Q8BJL0. Positions 393-807.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000047589.

PTM databases

PhosphoSitei Q8BJL0.

Proteomic databases

PaxDbi Q8BJL0.
PRIDEi Q8BJL0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000047615 ; ENSMUSP00000047589 ; ENSMUSG00000039354 . [Q8BJL0-1 ]
ENSMUST00000152225 ; ENSMUSP00000137833 ; ENSMUSG00000039354 . [Q8BJL0-1 ]
GeneIDi 54380.
KEGGi mmu:54380.
UCSCi uc007bko.2. mouse. [Q8BJL0-4 ]
uc007bkq.2. mouse. [Q8BJL0-3 ]
uc007bkr.2. mouse. [Q8BJL0-2 ]
uc007bks.2. mouse. [Q8BJL0-1 ]

Organism-specific databases

CTDi 50485.
MGIi MGI:1859183. Smarcal1.

Phylogenomic databases

eggNOGi COG0553.
GeneTreei ENSGT00630000089754.
HOVERGENi HBG054110.
InParanoidi Q3TEQ9.
KOi K14440.
OMAi SIHYLVA.
OrthoDBi EOG7XSTD5.
PhylomeDBi Q8BJL0.
TreeFami TF106474.

Miscellaneous databases

NextBioi 311200.
PROi Q8BJL0.
SOURCEi Search...

Gene expression databases

Bgeei Q8BJL0.
Genevestigatori Q8BJL0.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR010003. HARP_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view ]
Pfami PF07443. HARP. 2 hits.
PF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view ]
SMARTi SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 3 hits.
PROSITEi PS51467. HARP. 2 hits.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of HARP/SMARCAL1: a prokaryotic HepA-related SNF2 helicase protein from human and mouse."
    Coleman M.A., Eisen J.A., Mohrenweiser H.W.
    Genomics 65:274-282(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2; 3 AND 4).
    Strain: C57BL/6J and NOD.
    Tissue: Cerebellum, Embryo, Testis and Thymus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: Czech II.
    Tissue: Mammary gland.

Entry informationi

Entry nameiSMAL1_MOUSE
AccessioniPrimary (citable) accession number: Q8BJL0
Secondary accession number(s): Q3TEQ9
, Q3U4W0, Q3V3A8, Q8BVK7, Q8BXW4, Q8K309, Q9EQK8, Q9QYC4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2003
Last modified: September 3, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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