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Q8BJF9

- CHM2B_MOUSE

UniProt

Q8BJF9 - CHM2B_MOUSE

Protein

Charged multivesicular body protein 2b

Gene

Chmp2b

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4 By similarity.By similarity

    GO - Biological processi

    1. protein transport Source: UniProtKB-KW

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_198518. Endosomal Sorting Complex Required For Transport (ESCRT).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Charged multivesicular body protein 2b
    Alternative name(s):
    Chromatin-modifying protein 2b
    Short name:
    CHMP2b
    Gene namesi
    Name:Chmp2b
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 16

    Organism-specific databases

    MGIiMGI:1916192. Chmp2b.

    Subcellular locationi

    Cytoplasmcytosol By similarity. Late endosome membrane By similarity; Peripheral membrane protein By similarity

    GO - Cellular componenti

    1. cytosol Source: UniProtKB-SubCell
    2. late endosome membrane Source: UniProtKB-SubCell
    3. mitochondrion Source: Ensembl
    4. nucleus Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, Endosome, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 213212Charged multivesicular body protein 2bPRO_0000211470Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei199 – 1991Phosphoserine2 Publications

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ8BJF9.
    PaxDbiQ8BJF9.
    PRIDEiQ8BJF9.

    PTM databases

    PhosphoSiteiQ8BJF9.

    Expressioni

    Tissue specificityi

    In brain, it is expressed in all neuronal populations with a relatively enhanced expression in the hippocampus, frontal and temporal lobes and in both granule and Purkinje cells of the cerebellum. Not expressed in astrocytes or oligodendrocytes.1 Publication

    Gene expression databases

    BgeeiQ8BJF9.
    CleanExiMM_CHMP2B.
    GenevestigatoriQ8BJF9.

    Interactioni

    Subunit structurei

    Probable core component of the endosomal sorting required for transport complex III (ESCRT-III). ESCRT-III components are thought to multimerize to form a flat lattice on the perimeter membrane of the endosome. Several assembly forms of ESCRT-III may exist that interact and act sequentally. Interacts with CHMP2A. Interacts with VPS4A. Interacts with VPS4B; the interaction is direct By similarity.By similarity

    Protein-protein interaction databases

    IntActiQ8BJF9. 1 interaction.
    STRINGi10090.ENSMUSP00000004965.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BJF9.
    SMRiQ8BJF9. Positions 7-141.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili25 – 5531Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi201 – 21111MIT-interacting motifAdd
    BLAST

    Sequence similaritiesi

    Belongs to the SNF7 family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG268821.
    GeneTreeiENSGT00550000074737.
    HOGENOMiHOG000177218.
    HOVERGENiHBG102628.
    InParanoidiQ8BJF9.
    KOiK12192.
    OMAiKTMQDFQ.
    OrthoDBiEOG79GT8Q.
    PhylomeDBiQ8BJF9.
    TreeFamiTF314163.

    Family and domain databases

    InterProiIPR005024. Snf7.
    [Graphical view]
    PfamiPF03357. Snf7. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8BJF9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASLFKKKTV DDVIKEQNRE LRGTQRAIIR DRAALEKQEK QLELEIKKMA    50
    KIGNKEACRV LAKQLVHLRK QKTRTFAVSS KVTSMSTQTK VMNSQMKMAG 100
    AMSTTAKTMQ AVNKKMDPQK TLQTMQNFQK ENMKMEMTEE MINDTLDDIF 150
    DGSDDEEESQ DIVNQVLDEI GIEISGKMAK APSAARSLPS ASTSKATISD 200
    EEIERQLKAL GVD 213
    Length:213
    Mass (Da):23,935
    Last modified:March 1, 2003 - v1
    Checksum:iAC5B40ED59E4489B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti131 – 1311E → D in BAB23339. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK004506 mRNA. Translation: BAB23339.1.
    AK084111 mRNA. Translation: BAC39118.1.
    BC042626 mRNA. Translation: AAH42626.1.
    BC055809 mRNA. Translation: AAH55809.1.
    CCDSiCCDS28269.1.
    RefSeqiNP_081155.1. NM_026879.2.
    UniGeneiMm.432944.

    Genome annotation databases

    EnsembliENSMUST00000004965; ENSMUSP00000004965; ENSMUSG00000004843.
    GeneIDi68942.
    KEGGimmu:68942.
    UCSCiuc007zqj.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK004506 mRNA. Translation: BAB23339.1 .
    AK084111 mRNA. Translation: BAC39118.1 .
    BC042626 mRNA. Translation: AAH42626.1 .
    BC055809 mRNA. Translation: AAH55809.1 .
    CCDSi CCDS28269.1.
    RefSeqi NP_081155.1. NM_026879.2.
    UniGenei Mm.432944.

    3D structure databases

    ProteinModelPortali Q8BJF9.
    SMRi Q8BJF9. Positions 7-141.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8BJF9. 1 interaction.
    STRINGi 10090.ENSMUSP00000004965.

    PTM databases

    PhosphoSitei Q8BJF9.

    Proteomic databases

    MaxQBi Q8BJF9.
    PaxDbi Q8BJF9.
    PRIDEi Q8BJF9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000004965 ; ENSMUSP00000004965 ; ENSMUSG00000004843 .
    GeneIDi 68942.
    KEGGi mmu:68942.
    UCSCi uc007zqj.1. mouse.

    Organism-specific databases

    CTDi 25978.
    MGIi MGI:1916192. Chmp2b.

    Phylogenomic databases

    eggNOGi NOG268821.
    GeneTreei ENSGT00550000074737.
    HOGENOMi HOG000177218.
    HOVERGENi HBG102628.
    InParanoidi Q8BJF9.
    KOi K12192.
    OMAi KTMQDFQ.
    OrthoDBi EOG79GT8Q.
    PhylomeDBi Q8BJF9.
    TreeFami TF314163.

    Enzyme and pathway databases

    Reactomei REACT_198518. Endosomal Sorting Complex Required For Transport (ESCRT).

    Miscellaneous databases

    ChiTaRSi CHMP2B. mouse.
    NextBioi 328237.
    PROi Q8BJF9.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8BJF9.
    CleanExi MM_CHMP2B.
    Genevestigatori Q8BJF9.

    Family and domain databases

    InterProi IPR005024. Snf7.
    [Graphical view ]
    Pfami PF03357. Snf7. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Spinal ganglion.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6 and Czech II.
      Tissue: Brain and Mammary tumor.
    3. Cited for: TISSUE SPECIFICITY.
    4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    5. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCHM2B_MOUSE
    AccessioniPrimary (citable) accession number: Q8BJF9
    Secondary accession number(s): Q80UZ4, Q9CT65
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 30, 2005
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3