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Protein

Charged multivesicular body protein 2b

Gene

Chmp2b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4 (By similarity).By similarity

GO - Molecular functioni

  1. protein domain specific binding Source: MGI

GO - Biological processi

  1. cell separation after cytokinesis Source: MGI
  2. cognition Source: MGI
  3. endosome organization Source: MGI
  4. mitotic metaphase plate congression Source: MGI
  5. neuron cellular homeostasis Source: MGI
  6. nucleus organization Source: MGI
  7. positive regulation of viral release from host cell Source: MGI
  8. protein transport Source: UniProtKB-KW
  9. regulation of centrosome duplication Source: MGI
  10. regulation of mitotic spindle assembly Source: MGI
  11. vacuolar transport Source: InterPro
  12. viral budding via host ESCRT complex Source: MGI
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiREACT_334712. Endosomal Sorting Complex Required For Transport (ESCRT).

Names & Taxonomyi

Protein namesi
Recommended name:
Charged multivesicular body protein 2b
Alternative name(s):
Chromatin-modifying protein 2b
Short name:
CHMP2b
Gene namesi
Name:Chmp2b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1916192. Chmp2b.

Subcellular locationi

  1. Cytoplasmcytosol By similarity
  2. Late endosome membrane By similarity; Peripheral membrane protein By similarity

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. cytosol Source: MGI
  3. ESCRT III complex Source: MGI
  4. extracellular vesicular exosome Source: MGI
  5. intracellular Source: MGI
  6. late endosome membrane Source: UniProtKB-SubCell
  7. mitochondrion Source: MGI
  8. nucleoplasm Source: MGI
  9. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 213212Charged multivesicular body protein 2bPRO_0000211470Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei199 – 1991Phosphoserine2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ8BJF9.
PaxDbiQ8BJF9.
PRIDEiQ8BJF9.

PTM databases

PhosphoSiteiQ8BJF9.

Expressioni

Tissue specificityi

In brain, it is expressed in all neuronal populations with a relatively enhanced expression in the hippocampus, frontal and temporal lobes and in both granule and Purkinje cells of the cerebellum. Not expressed in astrocytes or oligodendrocytes.1 Publication

Gene expression databases

BgeeiQ8BJF9.
CleanExiMM_CHMP2B.
GenevestigatoriQ8BJF9.

Interactioni

Subunit structurei

Probable core component of the endosomal sorting required for transport complex III (ESCRT-III). ESCRT-III components are thought to multimerize to form a flat lattice on the perimeter membrane of the endosome. Several assembly forms of ESCRT-III may exist that interact and act sequentally. Interacts with CHMP2A. Interacts with VPS4A. Interacts with VPS4B; the interaction is direct (By similarity).By similarity

Protein-protein interaction databases

IntActiQ8BJF9. 1 interaction.
STRINGi10090.ENSMUSP00000004965.

Structurei

3D structure databases

ProteinModelPortaliQ8BJF9.
SMRiQ8BJF9. Positions 7-141.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili25 – 5531Sequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi201 – 21111MIT-interacting motifAdd
BLAST

Sequence similaritiesi

Belongs to the SNF7 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG268821.
GeneTreeiENSGT00550000074737.
HOGENOMiHOG000177218.
HOVERGENiHBG102628.
InParanoidiQ8BJF9.
KOiK12192.
OMAiNNDACRI.
OrthoDBiEOG79GT8Q.
PhylomeDBiQ8BJF9.
TreeFamiTF314163.

Family and domain databases

InterProiIPR005024. Snf7_fam.
[Graphical view]
PfamiPF03357. Snf7. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8BJF9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASLFKKKTV DDVIKEQNRE LRGTQRAIIR DRAALEKQEK QLELEIKKMA
60 70 80 90 100
KIGNKEACRV LAKQLVHLRK QKTRTFAVSS KVTSMSTQTK VMNSQMKMAG
110 120 130 140 150
AMSTTAKTMQ AVNKKMDPQK TLQTMQNFQK ENMKMEMTEE MINDTLDDIF
160 170 180 190 200
DGSDDEEESQ DIVNQVLDEI GIEISGKMAK APSAARSLPS ASTSKATISD
210
EEIERQLKAL GVD
Length:213
Mass (Da):23,935
Last modified:March 1, 2003 - v1
Checksum:iAC5B40ED59E4489B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti131 – 1311E → D in BAB23339 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004506 mRNA. Translation: BAB23339.1.
AK084111 mRNA. Translation: BAC39118.1.
BC042626 mRNA. Translation: AAH42626.1.
BC055809 mRNA. Translation: AAH55809.1.
CCDSiCCDS28269.1.
RefSeqiNP_081155.1. NM_026879.2.
UniGeneiMm.432944.

Genome annotation databases

EnsembliENSMUST00000004965; ENSMUSP00000004965; ENSMUSG00000004843.
GeneIDi68942.
KEGGimmu:68942.
UCSCiuc007zqj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004506 mRNA. Translation: BAB23339.1.
AK084111 mRNA. Translation: BAC39118.1.
BC042626 mRNA. Translation: AAH42626.1.
BC055809 mRNA. Translation: AAH55809.1.
CCDSiCCDS28269.1.
RefSeqiNP_081155.1. NM_026879.2.
UniGeneiMm.432944.

3D structure databases

ProteinModelPortaliQ8BJF9.
SMRiQ8BJF9. Positions 7-141.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BJF9. 1 interaction.
STRINGi10090.ENSMUSP00000004965.

PTM databases

PhosphoSiteiQ8BJF9.

Proteomic databases

MaxQBiQ8BJF9.
PaxDbiQ8BJF9.
PRIDEiQ8BJF9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000004965; ENSMUSP00000004965; ENSMUSG00000004843.
GeneIDi68942.
KEGGimmu:68942.
UCSCiuc007zqj.1. mouse.

Organism-specific databases

CTDi25978.
MGIiMGI:1916192. Chmp2b.

Phylogenomic databases

eggNOGiNOG268821.
GeneTreeiENSGT00550000074737.
HOGENOMiHOG000177218.
HOVERGENiHBG102628.
InParanoidiQ8BJF9.
KOiK12192.
OMAiNNDACRI.
OrthoDBiEOG79GT8Q.
PhylomeDBiQ8BJF9.
TreeFamiTF314163.

Enzyme and pathway databases

ReactomeiREACT_334712. Endosomal Sorting Complex Required For Transport (ESCRT).

Miscellaneous databases

ChiTaRSiChmp2b. mouse.
NextBioi328237.
PROiQ8BJF9.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BJF9.
CleanExiMM_CHMP2B.
GenevestigatoriQ8BJF9.

Family and domain databases

InterProiIPR005024. Snf7_fam.
[Graphical view]
PfamiPF03357. Snf7. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Spinal ganglion.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6 and Czech II.
    Tissue: Brain and Mammary tumor.
  3. Cited for: TISSUE SPECIFICITY.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  5. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCHM2B_MOUSE
AccessioniPrimary (citable) accession number: Q8BJF9
Secondary accession number(s): Q80UZ4, Q9CT65
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: March 1, 2003
Last modified: April 29, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.