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Protein

Choline dehydrogenase, mitochondrial

Gene

Chdh

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Choline + acceptor = betaine aldehyde + reduced acceptor.

Cofactori

FADBy similarity

Pathwayi: betaine biosynthesis via choline pathway

This protein is involved in step 1 of the subpathway that synthesizes betaine aldehyde from choline (cytochrome c reductase route).
Proteins known to be involved in this subpathway in this organism are:
  1. Choline dehydrogenase, mitochondrial (Chdh)
This subpathway is part of the pathway betaine biosynthesis via choline pathway, which is itself part of Amine and polyamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes betaine aldehyde from choline (cytochrome c reductase route), the pathway betaine biosynthesis via choline pathway and in Amine and polyamine biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei513 – 5131Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi44 – 7330FADBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

ReactomeiR-MMU-6798163. Choline catabolism.
UniPathwayiUPA00529; UER00385.

Names & Taxonomyi

Protein namesi
Recommended name:
Choline dehydrogenase, mitochondrial (EC:1.1.99.1)
Short name:
CDH
Short name:
CHD
Gene namesi
Name:Chdh
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1860776. Chdh.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial inner membrane Source: MGI
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Disruption phenotypei

Decreased testicular betaine and increased choline and phosphatidylcholine concentrations. Only one of eleven males was able to reproduce, impaired fertility was due to diminished sperm motility.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3434MitochondrionBy similarityAdd
BLAST
Chaini35 – 596562Choline dehydrogenase, mitochondrialPRO_0000012330Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei438 – 4381N6-succinyllysineCombined sources
Modified residuei486 – 4861N6-acetyllysine; alternateCombined sources
Modified residuei486 – 4861N6-succinyllysine; alternateCombined sources
Modified residuei498 – 4981N6-acetyllysine; alternateCombined sources
Modified residuei498 – 4981N6-succinyllysine; alternateCombined sources
Modified residuei582 – 5821N6-acetyllysineCombined sources

Post-translational modificationi

Acetylation of Lys-498 is observed in liver mitochondria from fasted mice but not from fed mice.

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ8BJ64.
PaxDbiQ8BJ64.
PeptideAtlasiQ8BJ64.
PRIDEiQ8BJ64.

PTM databases

iPTMnetiQ8BJ64.
PhosphoSiteiQ8BJ64.
SwissPalmiQ8BJ64.

Expressioni

Gene expression databases

BgeeiENSMUSG00000015970.
CleanExiMM_CHDH.
GenevisibleiQ8BJ64. MM.

Interactioni

Protein-protein interaction databases

IntActiQ8BJ64. 2 interactions.
MINTiMINT-1843656.
STRINGi10090.ENSMUSP00000065542.

Structurei

3D structure databases

ProteinModelPortaliQ8BJ64.
SMRiQ8BJ64. Positions 46-580.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GMC oxidoreductase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1238. Eukaryota.
COG2303. LUCA.
GeneTreeiENSGT00530000063260.
HOGENOMiHOG000139600.
HOVERGENiHBG023639.
InParanoidiQ8BJ64.
KOiK00108.
OMAiANLCNDR.
OrthoDBiEOG091G02M7.
PhylomeDBiQ8BJ64.
TreeFamiTF313911.

Family and domain databases

Gene3Di3.50.50.60. 3 hits.
InterProiIPR011533. BetA.
IPR023753. FAD/NAD-binding_dom.
IPR012132. GMC_OxRdtase.
IPR000172. GMC_OxRdtase_N.
IPR007867. GMC_OxRtase_C.
[Graphical view]
PfamiPF05199. GMC_oxred_C. 1 hit.
PF00732. GMC_oxred_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000137. Alcohol_oxidase. 1 hit.
SUPFAMiSSF51905. SSF51905. 2 hits.
TIGRFAMsiTIGR01810. betA. 1 hit.
PROSITEiPS00623. GMC_OXRED_1. 1 hit.
PS00624. GMC_OXRED_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8BJ64-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWQVLRGWRK GWQSPRGALA WAVQGQPCPP CSRAVASVGK DEYTFVVVGA
60 70 80 90 100
GSAGCVLASR LTEDPNHRVL LLEAGPKDLL MGSKRLQWKI HMPAALVSNL
110 120 130 140 150
CDDKYNWYYH TEPQPGMDSR VLYWPRGRVW GGSSSLNAMV YIRGHAEDYN
160 170 180 190 200
RWHREGAEGW DYAHCLPYFR KAQRHELGAN MYRGGDGPLH VSRGKTNHPL
210 220 230 240 250
HQAFLQAARQ AGYPFTEDMN GFQQEGFGWM DMTVHQGKRW STACAYLHPV
260 270 280 290 300
LSRPNLRAEV QTLVSRVLFE GTRAVGVEYI KDGQRHKAYV SREVILSGGA
310 320 330 340 350
INSPQLLMLS GVGNADDLRK LDIPVVCHLP GVGQNLQDHL EVYVQQACTQ
360 370 380 390 400
PITLHSAQKP LRKVCIGLEW LWSYTGDGAT AHLETGGFIR SRPGVPHPDI
410 420 430 440 450
QFHFLPSQVI DHGRKPTQQE AYQVHVGTMR ATSVGWLKLR SANPRDHPVI
460 470 480 490 500
HPNYLSTETD VEDFRQCVRL SREIFAQEAL APFRGKELQP GSHVQSDKEI
510 520 530 540 550
DAFVRAKADS AYHPSCTCKM GRSSDPTAVV DAQTKVIGVE NLRVVDASIM
560 570 580 590
PSVVSGNLNA PTVMIAEKAA DIIKGHPALE DKNVPVYKPQ TLDTQR
Length:596
Mass (Da):66,415
Last modified:March 1, 2003 - v1
Checksum:iD31F19A0E0DB9587
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 41V → A in AAH39186 (PubMed:15489334).Curated
Sequence conflicti12 – 121W → C in AAH39186 (PubMed:15489334).Curated
Sequence conflicti24 – 241Q → R in AAH39186 (PubMed:15489334).Curated
Sequence conflicti212 – 2121G → A in BAE37601 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030900 mRNA. Translation: BAC27176.1.
AK164042 mRNA. Translation: BAE37601.1.
BC039186 mRNA. Translation: AAH39186.1.
CCDSiCCDS26892.1.
RefSeqiNP_001129712.1. NM_001136240.1.
NP_758468.2. NM_172264.2.
NP_780552.1. NM_175343.5.
UniGeneiMm.259916.

Genome annotation databases

EnsembliENSMUST00000067620; ENSMUSP00000065542; ENSMUSG00000015970.
ENSMUST00000118917; ENSMUSP00000112916; ENSMUSG00000015970.
GeneIDi218865.
KEGGimmu:218865.
UCSCiuc007sup.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030900 mRNA. Translation: BAC27176.1.
AK164042 mRNA. Translation: BAE37601.1.
BC039186 mRNA. Translation: AAH39186.1.
CCDSiCCDS26892.1.
RefSeqiNP_001129712.1. NM_001136240.1.
NP_758468.2. NM_172264.2.
NP_780552.1. NM_175343.5.
UniGeneiMm.259916.

3D structure databases

ProteinModelPortaliQ8BJ64.
SMRiQ8BJ64. Positions 46-580.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BJ64. 2 interactions.
MINTiMINT-1843656.
STRINGi10090.ENSMUSP00000065542.

PTM databases

iPTMnetiQ8BJ64.
PhosphoSiteiQ8BJ64.
SwissPalmiQ8BJ64.

Proteomic databases

MaxQBiQ8BJ64.
PaxDbiQ8BJ64.
PeptideAtlasiQ8BJ64.
PRIDEiQ8BJ64.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000067620; ENSMUSP00000065542; ENSMUSG00000015970.
ENSMUST00000118917; ENSMUSP00000112916; ENSMUSG00000015970.
GeneIDi218865.
KEGGimmu:218865.
UCSCiuc007sup.2. mouse.

Organism-specific databases

CTDi55349.
MGIiMGI:1860776. Chdh.

Phylogenomic databases

eggNOGiKOG1238. Eukaryota.
COG2303. LUCA.
GeneTreeiENSGT00530000063260.
HOGENOMiHOG000139600.
HOVERGENiHBG023639.
InParanoidiQ8BJ64.
KOiK00108.
OMAiANLCNDR.
OrthoDBiEOG091G02M7.
PhylomeDBiQ8BJ64.
TreeFamiTF313911.

Enzyme and pathway databases

UniPathwayiUPA00529; UER00385.
ReactomeiR-MMU-6798163. Choline catabolism.

Miscellaneous databases

PROiQ8BJ64.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000015970.
CleanExiMM_CHDH.
GenevisibleiQ8BJ64. MM.

Family and domain databases

Gene3Di3.50.50.60. 3 hits.
InterProiIPR011533. BetA.
IPR023753. FAD/NAD-binding_dom.
IPR012132. GMC_OxRdtase.
IPR000172. GMC_OxRdtase_N.
IPR007867. GMC_OxRtase_C.
[Graphical view]
PfamiPF05199. GMC_oxred_C. 1 hit.
PF00732. GMC_oxred_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000137. Alcohol_oxidase. 1 hit.
SUPFAMiSSF51905. SSF51905. 2 hits.
TIGRFAMsiTIGR01810. betA. 1 hit.
PROSITEiPS00623. GMC_OXRED_1. 1 hit.
PS00624. GMC_OXRED_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCHDH_MOUSE
AccessioniPrimary (citable) accession number: Q8BJ64
Secondary accession number(s): Q3TPY4, Q8CHT7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: March 1, 2003
Last modified: September 7, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.